FastQCFastQC Report
Wed 25 May 2016
SRR1780341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533383
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT199833.746463610576265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC61541.1537675553964037No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG50540.9475367606391654No Hit
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG34070.6387530161253733RNA PCR Primer, Index 14 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC10870.20379352172828907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCA207.842384E-444.0117263
CTTTATA1300.038.9334452
TCTTCTG4750.035.66262444
GGCTTAA250.00235287235.19617513
AAATTCG250.00235287235.19617530
ATGCCGT27300.033.60389344
GTCTTCT5250.032.26618643
CCGTCTT5350.031.66307841
GCATTAC353.2157515E-431.428143
ATATCTC353.2175204E-431.4251548
CGCTAAT353.2175204E-431.42515413
CTTAATA502.3055109E-630.8893181
TTAATAC502.3532903E-630.8082072
TCTTTAT1700.029.851021
ATCTAAT300.005661470329.4183961
TTTATAC1800.029.3411483
TAGCTTG300.00574259129.3328975
TATTGAA300.00574522329.33014719
ACCTAGA300.00574522329.33014718
CCATATG300.00574522329.3301478
Produced by FastQC (version 0.11.5)