FastQCFastQC Report
Wed 25 May 2016
SRR1780340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52459
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG150.002213052770.064
CCGTGGA150.002213052770.02
CCTACCG150.002213052770.03
CTGCGTT150.002213052770.023
CTACCGA150.002213052770.04
TCCAAGA150.002213052770.030
GTGAGTC150.002213052770.068
CAGCACT252.3699876E-455.9999961
CAGGTCG200.006915287652.50000454
TGTCAAC200.006915287652.50000435
GAAATAC200.006915287652.50000444
GCCTTAA200.006915287652.50000460
AAGAGAG200.006915287652.50000425
ATAGCGG200.006915287652.50000451
AGCGGTT200.006915287652.50000453
TGGCAGT200.006915287652.50000430
CTAACTA200.006915287652.50000442
GCCTGCG200.006915287652.50000421
GTATGTG200.006915287652.50000415
CATAGCG200.006915287652.50000450
Produced by FastQC (version 0.11.5)