##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780340_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52459 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.141767856802456 34.0 33.0 34.0 21.0 34.0 2 31.108980346556358 34.0 33.0 34.0 21.0 34.0 3 31.320536037667512 34.0 34.0 34.0 23.0 34.0 4 31.122438475762024 34.0 34.0 34.0 21.0 34.0 5 30.98696124592539 34.0 33.0 34.0 21.0 34.0 6 34.386377933243104 38.0 36.0 38.0 23.0 38.0 7 34.693284279151335 38.0 36.0 38.0 26.0 38.0 8 34.56238205074439 38.0 36.0 38.0 24.0 38.0 9 34.868068396271376 38.0 37.0 38.0 26.0 38.0 10-11 34.745734764292116 38.0 36.5 38.0 25.0 38.0 12-13 34.75405554814236 38.0 36.5 38.0 25.0 38.0 14-15 34.743714138660664 38.0 37.0 38.0 25.0 38.0 16-17 34.801273375397926 38.0 37.0 38.0 25.0 38.0 18-19 34.78290665090833 38.0 37.0 38.0 25.0 38.0 20-21 34.79768962427801 38.0 37.0 38.0 25.5 38.0 22-23 34.79293352904173 38.0 37.0 38.0 25.0 38.0 24-25 34.85145542232982 38.0 37.0 38.0 25.5 38.0 26-27 34.88568215177567 38.0 37.0 38.0 25.0 38.0 28-29 34.959225299757904 38.0 37.0 38.0 26.0 38.0 30-31 34.96646905202158 38.0 37.0 38.0 26.0 38.0 32-33 35.05001048437828 38.0 37.0 38.0 26.0 38.0 34-35 35.02073047522828 38.0 37.0 38.0 26.0 38.0 36-37 34.97376046055014 38.0 37.0 38.0 26.0 38.0 38-39 34.97965077489087 38.0 37.0 38.0 26.0 38.0 40-41 35.002154063173144 38.0 37.0 38.0 26.0 38.0 42-43 35.053031891572466 38.0 37.0 38.0 26.0 38.0 44-45 35.06187689433653 38.0 37.0 38.0 26.0 38.0 46-47 35.0012867191521 38.0 37.0 38.0 26.0 38.0 48-49 35.00011437503574 38.0 37.0 38.0 26.0 38.0 50-51 34.918240911950285 38.0 37.0 38.0 26.0 38.0 52-53 34.94707295221029 38.0 37.0 38.0 26.0 38.0 54-55 34.93831373072304 38.0 37.0 38.0 26.0 38.0 56-57 34.84180026306258 38.0 37.0 38.0 25.0 38.0 58-59 34.92587544558607 38.0 37.0 38.0 25.5 38.0 60-61 34.94387045120952 38.0 37.0 38.0 25.5 38.0 62-63 34.8261785394308 38.0 37.0 38.0 25.0 38.0 64-65 34.82604510188909 38.0 37.0 38.0 25.0 38.0 66-67 34.744934139041916 38.0 37.0 38.0 24.5 38.0 68-69 34.7026630320822 38.0 36.5 38.0 24.0 38.0 70-71 34.660982862807145 38.0 36.5 38.0 24.0 38.0 72-73 34.722812100878784 38.0 37.0 38.0 24.0 38.0 74-75 34.7287500714844 38.0 37.0 38.0 24.0 38.0 76 33.374235116948476 37.0 34.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4931032413167493 1101 2 0.05412098788962183 1101 3 -0.5731366140882557 1101 4 0.091911336996926 1101 5 0.2553446289440089 1101 6 0.4032903237150265 1101 7 0.24505089368690847 1101 8 0.603376532644603 1101 9 -1.0145269139640547 1101 10-11 0.08892137965719371 1101 12-13 -0.19146230515820406 1101 14-15 -0.023946418721969565 1101 16-17 0.22679595909161776 1101 18-19 -0.1970775518819181 1101 20-21 -0.6500515075548634 1101 22-23 -0.1884669939809811 1101 24-25 -0.30056652696620034 1101 26-27 -0.06950559364656783 1101 28-29 -0.20258673470492283 1101 30-31 -0.10874736018390507 1101 32-33 0.14237665653372034 1101 34-35 -0.547808293325545 1101 36-37 -0.6481008965871453 1101 38-39 -0.10273098419066429 1101 40-41 0.018115035337388008 1101 42-43 -0.6571891965299557 1101 44-45 -0.10347038152232102 1101 46-47 -0.49917285364514896 1101 48-49 -0.48589305535045924 1101 50-51 -0.17644526214312606 1101 52-53 -0.18056844649870385 1101 54-55 0.0012128252726455457 1101 56-57 -0.13097777003421385 1101 58-59 -0.09695539219545424 1101 60-61 -0.43921808247025496 1101 62-63 -0.47195364813563145 1101 64-65 -0.1532470560575092 1101 66-67 -0.6016802517238915 1101 68-69 -0.5514005244716529 1101 70-71 -0.28772081213458023 1101 72-73 0.3880468259386447 1101 74-75 -0.29116175419544277 1101 76 -1.2023388634218257 1102 1 1.0761596022530355 1102 2 0.3826691810867082 1102 3 0.23228733047012184 1102 4 -0.217154499931155 1102 5 -0.42540637876407317 1102 6 -0.33873640706780606 1102 7 -0.6645028861023548 1102 8 -0.38467240307423367 1102 9 -0.6041952542511666 1102 10-11 -0.44392636802685104 1102 12-13 -0.4585049471212912 1102 14-15 -0.04569169840517162 1102 16-17 -0.2572044652065273 1102 18-19 -0.263020554498425 1102 20-21 0.04399417521155158 1102 22-23 -0.05419431170957267 1102 24-25 -0.18324809121201469 1102 26-27 -0.3906639275692001 1102 28-29 -0.208703699558896 1102 30-31 -0.4036168885058018 1102 32-33 -0.30695507389553 1102 34-35 -0.3120929973775972 1102 36-37 -0.12177673280806545 1102 38-39 -0.4923781095707582 1102 40-41 -0.2606842009259509 1102 42-43 -0.16654143470265126 1102 44-45 -0.2711565423313189 1102 46-47 -0.3014499203306755 1102 48-49 -0.04253685706180477 1102 50-51 0.1524741919266006 1102 52-53 0.08428198840688594 1102 54-55 -0.21505547258465185 1102 56-57 -0.3026594049963762 1102 58-59 0.055279244676057715 1102 60-61 -0.08952569862186976 1102 62-63 -0.5037229713800997 1102 64-65 0.02349079840823265 1102 66-67 -0.05755324514726823 1102 68-69 0.004306285513500541 1102 70-71 -0.0123866773492054 1102 72-73 -0.261323798486778 1102 74-75 -0.38471949410067907 1102 76 0.3592904414363858 1103 1 0.21714061953929686 1103 2 -0.3281442384341915 1103 3 0.16723716813720912 1103 4 0.5357777297333577 1103 5 0.39117205289523227 1103 6 -0.4277123934961935 1103 7 -0.41456869674320274 1103 8 -0.647534727213575 1103 9 0.40727042184348505 1103 10-11 0.29035951339700716 1103 12-13 0.18714131337184625 1103 14-15 -0.1954620923915371 1103 16-17 -0.02264071663242362 1103 18-19 -0.009225474209443973 1103 20-21 0.18284000281692414 1103 22-23 -0.24465030632772766 1103 24-25 -0.15396966832745562 1103 26-27 0.329626224234012 1103 28-29 0.009422013389709605 1103 30-31 -0.2191423499115146 1103 32-33 -0.2605658843901395 1103 34-35 0.030444075950086358 1103 36-37 -0.1949727900194418 1103 38-39 -0.5729742077309865 1103 40-41 -0.15986229914467742 1103 42-43 -0.3343878180423161 1103 44-45 -0.16455792874215547 1103 46-47 0.1959030294484947 1103 48-49 -0.24807080072788779 1103 50-51 -0.4168024760075255 1103 52-53 0.3007258738671226 1103 54-55 0.09782293860023117 1103 56-57 0.02007597772906422 1103 58-59 -0.3652799995252991 1103 60-61 -0.22714306093138248 1103 62-63 -0.05351968962280296 1103 64-65 0.3239383107660103 1103 66-67 0.04557934135716124 1103 68-69 -0.02470889480765237 1103 70-71 0.11813776033542922 1103 72-73 0.1340372825626801 1103 74-75 -0.031038485589171216 1103 76 0.17899853468275495 1104 1 -0.3161104879919172 1104 2 -0.3670085155183642 1104 3 -0.3431585225595306 1104 4 -0.8355472803351098 1104 5 -0.39935352100730626 1104 6 -1.0145529090016296 1104 7 -0.20881960290510904 1104 8 -0.8361926007536482 1104 9 -0.5386870892318285 1104 10-11 -0.5057414733126251 1104 12-13 -0.29975709581059107 1104 14-15 -0.2123897001241133 1104 16-17 -1.0109109542482102 1104 18-19 -0.45742321887315995 1104 20-21 -0.8598251200183569 1104 22-23 -0.6985996619459343 1104 24-25 -1.0409511423508135 1104 26-27 -0.5502443959834693 1104 28-29 -1.0141130248704542 1104 30-31 -0.619640738957024 1104 32-33 -0.642311756319252 1104 34-35 -1.2174163751171747 1104 36-37 -0.8981921818841201 1104 38-39 -0.8112570309287364 1104 40-41 -0.9852625206509202 1104 42-43 -0.7771989336282985 1104 44-45 -0.7411286093704277 1104 46-47 -0.5222680337814651 1104 48-49 -0.8011316142599085 1104 50-51 -0.6545063624352352 1104 52-53 -0.609274629556154 1104 54-55 -0.6357151201996061 1104 56-57 -0.6994417427703326 1104 58-59 -0.5921476998877608 1104 60-61 -0.3728840999970302 1104 62-63 -0.7915130444355327 1104 64-65 -0.5958286042561411 1104 66-67 -0.650000602259432 1104 68-69 -0.7083199499828439 1104 70-71 -0.9324688647149131 1104 72-73 -0.9340381302444101 1104 74-75 -0.48766857016428844 1104 76 -0.25875317695638955 1105 1 0.4230486371966222 1105 2 0.03947715567318966 1105 3 0.08416048196535542 1105 4 0.4140756457584054 1105 5 0.23356503102889192 1105 6 0.3332977697314021 1105 7 0.35283198080529843 1105 8 0.17163168280593055 1105 9 -0.1913077528819045 1105 10-11 -0.12909177497174795 1105 12-13 -0.9417725558407639 1105 14-15 -0.14767439092351253 1105 16-17 -0.49186872197068254 1105 18-19 0.1834138851992364 1105 20-21 -0.21418032363826 1105 22-23 -0.10767771624457367 1105 24-25 -0.3667429975544323 1105 26-27 0.2749347141221108 1105 28-29 0.01067958448097528 1105 30-31 -0.42327950215461385 1105 32-33 -0.14212321936601313 1105 34-35 -0.3306328155395022 1105 36-37 -0.16671593754023917 1105 38-39 0.33019281824170577 1105 40-41 0.23377028477894157 1105 42-43 0.1766095128271985 1105 44-45 0.03828315533545634 1105 46-47 -0.13524880301223874 1105 48-49 -0.4242462447274775 1105 50-51 -0.283512772691644 1105 52-53 -0.16182930527259742 1105 54-55 -0.374779976911519 1105 56-57 -0.0579447593615825 1105 58-59 -0.07734754905820296 1105 60-61 0.05829991206933727 1105 62-63 -0.08063446223341941 1105 64-65 -0.17477845342658327 1105 66-67 -0.4356340864223256 1105 68-69 0.15522643010765336 1105 70-71 0.07002924991555659 1105 72-73 0.0780753690014393 1105 74-75 -0.13274775568464747 1105 76 -0.6672184861569974 1106 1 0.3867741273926981 1106 2 0.018011727396615385 1106 3 0.3267301414080812 1106 4 -0.10315861636749801 1106 5 0.3349582198833829 1106 6 0.3029572124558655 1106 7 -0.2293661616095619 1106 8 -0.45365211492368474 1106 9 -0.5602964009727174 1106 10-11 -0.22803398343408077 1106 12-13 -0.44512652900897365 1106 14-15 -0.3036599430391007 1106 16-17 -0.5531174319810077 1106 18-19 -0.2611681581444145 1106 20-21 -0.2258676301398168 1106 22-23 -0.40009257692568667 1106 24-25 -0.6268823164398825 1106 26-27 -0.5251684850522906 1106 28-29 -0.3364308386356356 1106 30-31 -0.3419585527222111 1106 32-33 -0.04666398303989894 1106 34-35 -0.4196937030524026 1106 36-37 -0.6126911697383761 1106 38-39 -0.4817782859894635 1106 40-41 -0.45330401862663194 1106 42-43 -0.504324439701179 1106 44-45 0.060187180103255855 1106 46-47 -0.1799856451175046 1106 48-49 -0.5578117762032235 1106 50-51 -0.6672847025162056 1106 52-53 -0.16714406275453797 1106 54-55 -0.5578811121024358 1106 56-57 -0.33157737030069967 1106 58-59 -0.6916922343007172 1106 60-61 -0.4001108206448052 1106 62-63 -0.3871102104274371 1106 64-65 -0.6022810334059443 1106 66-67 -0.830061331004373 1106 68-69 -0.9636590188087553 1106 70-71 -0.3572411319213913 1106 72-73 -0.45182659178287565 1106 74-75 -0.6675774770468763 1106 76 -0.203338197199308 1107 1 0.019382823044875153 1107 2 0.23917877545153488 1107 3 -0.038601666372237275 1107 4 -0.42501216327825375 1107 5 -0.19450402267954559 1107 6 0.0874537799615851 1107 7 0.6523844104728198 1107 8 -0.4961006275094988 1107 9 0.20148849605245545 1107 10-11 0.14110217217232446 1107 12-13 0.6006400613800409 1107 14-15 0.00886866107989448 1107 16-17 0.24076128655446638 1107 18-19 0.5501018647388776 1107 20-21 0.5927250472057253 1107 22-23 0.24862023771962072 1107 24-25 0.5554117339033624 1107 26-27 0.15909924950148024 1107 28-29 0.5828769416847237 1107 30-31 0.01811009716337253 1107 32-33 0.3208989460218845 1107 34-35 0.6164046951169411 1107 36-37 0.7421715304904595 1107 38-39 0.6894745744224409 1107 40-41 0.7163470111216554 1107 42-43 0.45900507974962323 1107 44-45 0.5931105210643537 1107 46-47 0.5359468491616752 1107 48-49 0.6081493221949401 1107 50-51 0.5878640389025591 1107 52-53 0.7367747015475246 1107 54-55 0.7440925721080873 1107 56-57 0.660299059905249 1107 58-59 0.6448924338914992 1107 60-61 0.56607911070531 1107 62-63 0.3341140458654692 1107 64-65 0.49583109474050957 1107 66-67 0.5395896987439173 1107 68-69 0.6638581436626438 1107 70-71 -0.04356836304267375 1107 72-73 0.4834994951736533 1107 74-75 0.528217717461132 1107 76 0.3447167227091583 1108 1 -0.37849533368514443 1108 2 0.8962342297603101 1108 3 0.5581830251143529 1108 4 0.574402051769912 1108 5 0.6885667922124092 1108 6 0.7869786432784309 1108 7 0.6364705859479542 1108 8 0.784230015552204 1108 9 0.7240086730896849 1108 10-11 0.8929083424404851 1108 12-13 0.9976720842907483 1108 14-15 0.7782971953157656 1108 16-17 0.8218274151668084 1108 18-19 1.1772497428956115 1108 20-21 0.8552543364533136 1108 22-23 0.8941715264465131 1108 24-25 0.9629065269260124 1108 26-27 1.0549922461169459 1108 28-29 0.7514040179669479 1108 30-31 1.0740200164552292 1108 32-33 0.7747195656521839 1108 34-35 0.8111131043853561 1108 36-37 0.9050522907091505 1108 38-39 0.6806389847322478 1108 40-41 0.668914701827191 1108 42-43 0.7769235905541052 1108 44-45 0.7236917499110049 1108 46-47 0.974032634113513 1108 48-49 0.33025944976589017 1108 50-51 0.734700800162642 1108 52-53 0.7888282032397953 1108 54-55 0.7833889792937185 1108 56-57 0.4330144985930957 1108 58-59 0.7039428113973614 1108 60-61 0.6636773924027892 1108 62-63 0.8646074069693483 1108 64-65 0.7068113082265839 1108 66-67 0.8813789463356869 1108 68-69 0.9057547851622516 1108 70-71 0.9820434567959069 1108 72-73 1.0260177195943783 1108 74-75 0.8993938434699871 1108 76 0.48764825095700814 1109 1 -0.4903500556138347 1109 2 -0.28078428223530594 1109 3 -0.4174735049627465 1109 4 -0.24278709933963327 1109 5 -0.6275460800822188 1109 6 -0.47062379889604955 1109 7 0.011328712831442544 1109 8 0.3448342971850167 1109 9 0.1273196980984892 1109 10-11 0.4713984211066844 1109 12-13 0.18731241147950328 1109 14-15 0.15951515156015006 1109 16-17 0.20584369632997124 1109 18-19 0.01347924467636119 1109 20-21 -0.009114964161142325 1109 22-23 -0.2465666051334665 1109 24-25 -0.5358606446132583 1109 26-27 -0.011012998296187959 1109 28-29 -0.3014119187869966 1109 30-31 -0.34811610346179833 1109 32-33 0.2124519440326864 1109 34-35 -0.466253730572177 1109 36-37 -0.5474348917885976 1109 38-39 -0.037454240581830334 1109 40-41 -0.06606631652825001 1109 42-43 -0.020285912009399 1109 44-45 -0.18312207342271591 1109 46-47 0.2650779943733852 1109 48-49 0.0014073500437632447 1109 50-51 -0.09642126942367923 1109 52-53 -0.7059469523314235 1109 54-55 -0.19422442135596896 1109 56-57 0.0825781164554158 1109 58-59 0.012521731987547469 1109 60-61 -0.13124257159079633 1109 62-63 0.12933285802802175 1109 64-65 -0.328373224668411 1109 66-67 0.12972539070185718 1109 68-69 -0.06862977904267353 1109 70-71 -0.24614722067268247 1109 72-73 -0.40476776825345695 1109 74-75 -0.27817259228595503 1109 76 -0.6541465907321609 1110 1 0.34010746072603126 1110 2 0.0422222537124064 1110 3 0.4769055800045727 1110 4 0.5670168222289931 1110 5 -0.33486634152012584 1110 6 0.33839580894709087 1110 7 -0.2497170388025438 1110 8 0.3547689377078953 1110 9 0.03163342358868704 1110 10-11 0.17845724463609258 1110 12-13 -0.25530196760546175 1110 14-15 -0.16453713602155773 1110 16-17 0.40460186626461336 1110 18-19 0.5065511401012017 1110 20-21 0.28395693126369537 1110 22-23 0.38271444061817306 1110 24-25 0.2427668063671362 1110 26-27 0.18395432196524553 1110 28-29 -0.005202768460200957 1110 30-31 0.6092695174532281 1110 32-33 0.3114061923986924 1110 34-35 0.20995541975462118 1110 36-37 0.5881860232440843 1110 38-39 0.6698006613789573 1110 40-41 0.6307310690926613 1110 42-43 0.3169036304742576 1110 44-45 0.5105380572962375 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0.7837708224313857 2116 26-27 0.5418258079090208 2116 28-29 0.6250783560377897 2116 30-31 1.4352132925536338 2116 32-33 0.6824102084629473 2116 34-35 0.9542124478670644 2116 36-37 0.08115791079428192 2116 38-39 0.4793187336681157 2116 40-41 0.16032946266696513 2116 42-43 0.4716425862975555 2116 44-45 1.019413559304276 2116 46-47 -0.023803019879707676 2116 48-49 0.8918711660922725 2116 50-51 1.0001217654609675 2116 52-53 0.7442066030666439 2116 54-55 0.7960504034333908 2116 56-57 0.4848145130220658 2116 58-59 1.092803643949125 2116 60-61 0.570466224377391 2116 62-63 0.8467515524134015 2116 64-65 0.11886671548435856 2116 66-67 0.8389938370362131 2116 68-69 0.8938869545583898 2116 70-71 0.8618140361191919 2116 72-73 0.3156432877351989 2116 74-75 0.5198036263139372 2116 76 1.3290016886599219 2117 1 -0.26998262936405837 2117 2 -0.5707507192605661 2117 3 -1.0012926181936237 2117 4 -0.16091110569893274 2117 5 -0.3606321072858911 2117 6 -0.8900726595213797 2117 7 -1.076023345108851 2117 8 -1.809555386616431 2117 9 -0.2550332430779818 2117 10-11 -0.27568689950805236 2117 12-13 -0.3779145802180821 2117 14-15 -0.6513839828684027 2117 16-17 -1.603686422023678 2117 18-19 -1.2887641752141121 2117 20-21 -2.115412438105672 2117 22-23 -1.1768351089313782 2117 24-25 -1.3005665269662003 2117 26-27 -1.1512709032599702 2117 28-29 -0.616373939631373 2117 30-31 -1.0462259780422656 2117 32-33 -1.2870622760697685 2117 34-35 -1.0987833189841183 2117 36-37 -1.4163184812604257 2117 38-39 -0.49479393321563947 2117 40-41 -0.04782748946588811 2117 42-43 -0.6950783515077177 2117 44-45 -1.1904529336947505 2117 46-47 -1.2703839381473756 2117 48-49 -1.0121503252786184 2117 50-51 -1.156260520439396 2117 52-53 -0.5472294112609362 2117 54-55 -0.926515536000025 2117 56-57 -1.5238960047245982 2117 58-59 -0.3874959327360017 2117 60-61 -0.5997552018681276 2117 62-63 -1.2051045205270086 2117 64-65 -1.2754673776449152 2117 66-67 -1.3597199369787702 2117 68-69 -1.164725875138771 2117 70-71 -0.9990619000579528 2117 72-73 -2.0923671323234103 2117 74-75 -1.7372787572744741 2117 76 -1.0013467320499458 2118 1 -0.5293549048653645 2118 2 0.25706096338982576 2118 3 -0.6364277533287606 2118 4 -0.372768124007564 2118 5 -0.12174806195023535 2118 6 -0.7549375243862428 2118 7 -1.0707923076197474 2118 8 -0.6626504171308198 2118 9 -1.061484855981206 2118 10-11 -1.4406825403101422 2118 12-13 -0.19809766653019523 2118 14-15 -0.3394833725806947 2118 16-17 0.34051584475923846 2118 18-19 -1.3094703652751463 2118 20-21 -0.19496779991909818 2118 22-23 -0.9525543765861286 2118 24-25 -0.02637297857909715 2118 26-27 -0.6867983662067942 2118 28-29 -0.036600617922566414 2118 30-31 -0.11793478362204013 2118 32-33 -0.11160456028947863 2118 34-35 0.05662208642128519 2118 36-37 -0.5058999982612917 2118 38-39 -0.31148966120168353 2118 40-41 -0.7527097911223848 2118 42-43 -0.5867522835042394 2118 44-45 -0.38225764162849174 2118 46-47 -0.2562165449477263 2118 48-49 0.167230668618501 2118 50-51 -0.23646976193896307 2118 52-53 -1.3846313293080144 2118 54-55 -0.6409880730532009 2118 56-57 -0.32707821919712643 2118 58-59 -0.3308263599373902 2118 60-61 -0.274559037962284 2118 62-63 -0.639062672227098 2118 64-65 -0.38793468366060324 2118 66-67 -0.2762412970485144 2118 68-69 0.7548469234662818 2118 70-71 0.20625632138930428 2118 72-73 -0.15666530146029345 2118 74-75 -0.5563720441096933 2118 76 -0.7092804722417512 2119 1 0.1455042861228577 2119 2 1.2196825711727222 2119 3 0.00642938952838179 2119 4 0.6479692031813755 2119 5 0.8813241346703506 2119 6 -0.03271530216402141 2119 7 0.26996550087999793 2119 8 1.070324493263449 2119 9 0.8798873918426509 2119 10-11 0.45972708590593925 2119 12-13 0.3339043816008811 2119 14-15 0.4732406417562274 2119 16-17 1.2044431361058798 2119 18-19 0.8281384416885018 2119 20-21 0.638877566184334 2119 22-23 0.7338255517292822 2119 24-25 0.7708620444623691 2119 26-27 0.5898273378382655 2119 28-29 0.5071781839207503 2119 30-31 0.4016504698341876 2119 32-33 0.8758506368431398 2119 34-35 0.9693204991196964 2119 36-37 0.5762812613393109 2119 38-39 0.09186415344245091 2119 40-41 0.760731069092671 2119 42-43 0.22341156698219322 2119 44-45 0.02276027951845805 2119 46-47 0.8211004033369633 2119 48-49 0.15033358720529577 2119 50-51 0.3652967511178744 2119 52-53 0.7198233557918314 2119 54-55 0.5546084451239608 2119 56-57 0.11992638909779885 2119 58-59 0.7720922268521662 2119 60-61 0.4065725544596219 2119 62-63 0.7255046600821728 2119 64-65 0.11513751295997565 2119 66-67 -0.3128516401104662 2119 68-69 0.30438609397319993 2119 70-71 0.527428876432829 2119 72-73 0.5910305510742688 2119 74-75 0.3478778278821153 2119 76 0.14043790973469328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 8.0 13 89.0 14 404.0 15 741.0 16 849.0 17 819.0 18 738.0 19 536.0 20 403.0 21 286.0 22 194.0 23 184.0 24 163.0 25 162.0 26 189.0 27 227.0 28 302.0 29 351.0 30 462.0 31 649.0 32 802.0 33 1302.0 34 1944.0 35 3366.0 36 7791.0 37 29498.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.152137860043084 19.645818639318325 10.62734707104596 28.574696429592635 2 22.85975714367411 20.044225013820316 28.835852761203988 28.260165081301587 3 20.492193903810595 21.11744409920128 28.119102537219543 30.27125945976858 4 15.168035990011248 17.466974208429438 32.89235402886063 34.47263577269868 5 14.314035723136165 31.12907222783507 32.79704149907547 21.759850549953295 6 27.8636649573953 35.96522998913437 18.16847442764826 18.002630625822068 7 28.317352599172686 30.480947025295947 20.160506300158218 21.04119407537315 8 25.112945347795424 31.97354124173164 20.48456890142778 22.42894450904516 9 24.262757582111742 19.01484969214053 20.50553765798052 36.216855067767206 10-11 24.58872643397701 24.278960712175223 25.984101870031832 25.148210983815932 12-13 24.195085685964276 22.392725747726796 25.52374235116949 27.88844621513944 14-15 23.337272917897785 22.856897767780552 23.920585600183 29.88524371413866 16-17 23.755694923654662 25.860195581311118 24.603023313444787 25.78108618158943 18-19 22.840694637717075 25.53803923063726 25.766789302121655 25.85447682952401 20-21 22.610038315636974 25.25686726777102 26.168055052517204 25.965039364074798 22-23 23.55839798699937 25.25496101717532 25.656226767570868 25.530414228254443 24-25 22.76253836329324 26.032711260222268 25.43033607198002 25.77441430450447 26-27 22.000991250309767 26.622695819592444 26.115633161135364 25.26067976896243 28-29 22.570960178425057 25.54757048361578 27.284164776301495 24.597304561657676 30-31 22.66341333231667 25.25019539068606 26.762805238376636 25.32358603862064 32-33 22.23450694828342 25.859242456013266 26.616977067805337 25.289273527897976 34-35 22.82735088354715 25.414132941916545 26.605539564231112 25.15297661030519 36-37 22.5976476867649 25.112945347795424 26.698945843420574 25.590461122019097 38-39 22.000038125011915 25.693398654187078 27.42808669627709 24.87847652452391 40-41 22.17541317981662 24.623038944699672 28.01330562915801 25.188242246325704 42-43 21.564459863893706 25.294992279685086 27.010617815818065 26.12993004060314 44-45 21.263272269772585 25.091976591242688 27.69496177967556 25.94978935930917 46-47 21.651194265998207 24.93375779179931 27.796946186545686 25.618101755656795 48-49 21.202272250710077 25.162507863283707 28.101946281858215 25.533273604148 50-51 21.370022303131968 25.806820564631426 27.31657103642845 25.506586095808153 52-53 20.84580338931356 25.257820393068876 27.531024228445073 26.3653519891725 54-55 20.463600144875045 25.431289197277874 27.450008578127683 26.655102079719402 56-57 20.796240873825273 25.08816409005128 27.591071122209726 26.524523913913722 58-59 20.844850264015708 25.06052345641358 27.04302407594502 27.05160220362569 60-61 20.57702205531939 25.079585962370615 27.57963361863551 26.76375836367449 62-63 20.41594387998246 25.131054728454604 27.37566480489525 27.077336586667684 64-65 21.37574105491908 24.885148401608877 26.475914523723286 27.263196019748758 66-67 20.969709678034274 25.3321641663013 26.05844564326426 27.63968051240016 68-69 20.379725118664098 24.936617167692866 26.758992737185228 27.9246649764578 70-71 21.517756724298977 24.874664023332507 26.230008196877563 27.377571055490957 72-73 22.011475628586133 24.71549209859128 25.669570521740788 27.6034617510818 74-75 21.459616081130026 24.935664042395015 26.244305076345338 27.36041480012962 76 21.609256752892737 25.071007834689947 25.421757944299355 27.897977468117958 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.5 15 3.0 16 3.0 17 2.0 18 4.0 19 3.5 20 9.5 21 18.0 22 18.0 23 25.5 24 44.5 25 56.0 26 88.0 27 144.0 28 196.0 29 224.0 30 288.5 31 430.0 32 614.5 33 722.0 34 837.5 35 1097.0 36 1358.0 37 1475.0 38 1599.0 39 2020.5 40 2528.5 41 2790.5 42 2842.0 43 2914.0 44 3214.5 45 3298.0 46 3153.0 47 3233.5 48 3143.0 49 2863.5 50 2755.0 51 2565.5 52 2155.0 53 1760.5 54 1587.0 55 1521.5 56 1282.0 57 1041.5 58 975.0 59 901.0 60 817.5 61 790.0 62 772.0 63 783.5 64 806.0 65 829.5 66 865.0 67 888.0 68 829.5 69 740.0 70 633.5 71 558.0 72 500.0 73 383.5 74 235.5 75 146.0 76 119.0 77 52.5 78 18.0 79 23.0 80 15.5 81 5.0 82 2.5 83 3.0 84 2.0 85 2.5 86 3.0 87 2.0 88 4.0 89 4.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 7.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.15677004899064 #Duplication Level Percentage of deduplicated Percentage of total 1 75.09805086760161 48.18048380640119 2 13.388400285238887 17.17913036847824 3 5.0629902543380085 9.744753045235328 4 2.391846921797005 6.138126918164661 5 1.3816258616591395 4.432032635010199 6 0.8616591395293558 3.3168760365237615 7 0.6120751129070597 2.7488133590041746 8 0.3565486094604231 1.8300005718751788 9 0.2347278345614452 1.3553441735450542 >10 0.6120751129070597 5.074439085762214 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.001906250595703311 0.0 5 0.0 0.0 0.0 0.001906250595703311 0.0 6 0.0 0.0 0.0 0.001906250595703311 0.0 7 0.0 0.0 0.0 0.001906250595703311 0.0 8 0.0 0.0 0.0 0.001906250595703311 0.0 9 0.0 0.0 0.0 0.001906250595703311 0.0 10 0.0 0.0 0.0 0.001906250595703311 0.0 11 0.0 0.0 0.0 0.001906250595703311 0.0 12 0.0 0.0 0.0 0.001906250595703311 0.0 13 0.0 0.0 0.0 0.001906250595703311 0.0 14 0.0 0.0 0.0 0.001906250595703311 0.0 15 0.0 0.0 0.0 0.001906250595703311 0.0 16 0.0 0.0 0.0 0.001906250595703311 0.0 17 0.0 0.0 0.0 0.001906250595703311 0.0 18 0.0 0.0 0.0 0.001906250595703311 0.0 19 0.0 0.0 0.0 0.005718751787109934 0.0 20 0.0 0.0 0.0 0.005718751787109934 0.0 21 0.0 0.0 0.0 0.009531252978516556 0.0 22 0.0 0.0 0.0 0.013343754169923177 0.0 23 0.0 0.0 0.0 0.013343754169923177 0.0 24 0.0 0.0 0.0 0.013343754169923177 0.0 25 0.0 0.0 0.0 0.013343754169923177 0.0 26 0.0 0.0 0.0 0.013343754169923177 0.0 27 0.0 0.0 0.0 0.026687508339846355 0.0 28 0.0 0.0 0.0 0.038125011914066226 0.0 29 0.0 0.0 0.0 0.05718751787109933 0.0 30 0.0 0.0 0.0 0.07434377323242913 0.0 31 0.0 0.0 0.0 0.09150002859375894 0.0 32 0.0 0.0 0.0 0.12200003812501191 0.0 33 0.0 0.0 0.0 0.14106254408204502 0.0 34 0.0 0.0 0.0 0.171562553613298 0.0 35 0.0 0.0 0.0 0.22684382088869404 0.0 36 0.0 0.0 0.0 0.343125107226596 0.0 37 0.0 0.0 0.0 0.5032501572656741 0.0 38 0.0 0.0 0.0 0.716750223984445 0.0 39 0.0 0.0 0.0 0.9416877942774358 0.0 40 0.0 0.0 0.0 1.2276253836329323 0.0 41 0.0 0.0 0.0 1.6679692712403973 0.0 42 0.0 0.0 0.0 2.2226881945900607 0.0 43 0.0 0.0 0.0 2.754532110791285 0.0 44 0.0 0.0 0.0 3.253969766865552 0.0 45 0.0 0.0 0.0 3.8067824396195125 0.0 46 0.0 0.0 0.0 4.302407594502373 0.0 47 0.0 0.0 0.0 4.798032749385234 0.0 48 0.0 0.0 0.0 5.310814159629425 0.0 49 0.0 0.0 0.0 5.73400179187556 0.0 50 0.0 0.0 0.0 6.170533178291619 0.0 51 0.0 0.0 0.0 6.738595855811205 0.0 52 0.0 0.0 0.0 7.419127318477287 0.0 53 0.0 0.0 0.0 8.215940067481272 0.0 54 0.0 0.0 0.0 8.970815303379782 0.0 55 0.0 0.0 0.0 9.582721744600546 0.0 56 0.0 0.0 0.0 10.116471911397472 0.0 57 0.0 0.0 0.0 10.61590956747174 0.0 58 0.0 0.0 0.0 11.195409748565547 0.0 59 0.0 0.0 0.0 11.63575363617301 0.0 60 0.0 0.0 0.0 12.127566289864465 0.0 61 0.0 0.0 0.0 12.697535217979755 0.0 62 0.0 0.0 0.0 13.307535408604815 0.0 63 0.0 0.0 0.0 13.95566061114394 0.0 64 0.0 0.0 0.0 14.630473322022914 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTG 15 0.0022130527 70.0 64 CCGTGGA 15 0.0022130527 70.0 2 CCTACCG 15 0.0022130527 70.0 3 CTGCGTT 15 0.0022130527 70.0 23 CTACCGA 15 0.0022130527 70.0 4 TCCAAGA 15 0.0022130527 70.0 30 GTGAGTC 15 0.0022130527 70.0 68 CAGCACT 25 2.3699876E-4 55.999996 1 CAGGTCG 20 0.0069152876 52.500004 54 TGTCAAC 20 0.0069152876 52.500004 35 GAAATAC 20 0.0069152876 52.500004 44 GCCTTAA 20 0.0069152876 52.500004 60 AAGAGAG 20 0.0069152876 52.500004 25 ATAGCGG 20 0.0069152876 52.500004 51 AGCGGTT 20 0.0069152876 52.500004 53 TGGCAGT 20 0.0069152876 52.500004 30 CTAACTA 20 0.0069152876 52.500004 42 GCCTGCG 20 0.0069152876 52.500004 21 GTATGTG 20 0.0069152876 52.500004 15 CATAGCG 20 0.0069152876 52.500004 50 >>END_MODULE