FastQCFastQC Report
Wed 25 May 2016
SRR1780340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52459
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG15062.8708133971291865RNA PCR Primer, Index 18 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT10852.0682818963380925No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG2590.4937189042871576No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1560.29737509292971653No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT1340.2554375798242437RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT1080.2058750643359576No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT910.17346880420900132No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC660.12581253931641853No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC600.11437503574219868No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATATA207.858393E-570.000012
CTCCGCC150.002213052770.09
TCACAAG150.002213052770.055
GCGTGAC150.002213052770.011
TCTTTAT352.9211515E-760.01
CTTTATA352.9211515E-760.02
TTTAGAC252.3699876E-455.99999642
CTTAATA200.006915287652.5000041
CGGAACT200.006915287652.50000434
TTAATAC200.006915287652.5000042
CTTTTGG200.006915287652.50000431
TTAGACG200.006915287652.50000443
GCCGGAA200.006915287652.50000432
TCGGAAT200.006915287652.50000468
CGAGGGT200.006915287652.50000428
CAATGAC200.006915287652.50000455
AACTCGT200.006915287652.50000437
TTCCTTC200.006915287652.50000425
TTTATAC506.050686E-848.9999963
ATATACA451.6549966E-646.6666684
Produced by FastQC (version 0.11.5)