##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780340_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52459 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.296250405078254 34.0 34.0 34.0 33.0 34.0 2 33.476448273890085 34.0 34.0 34.0 34.0 34.0 3 33.55681579900494 34.0 34.0 34.0 34.0 34.0 4 33.54684610838941 34.0 34.0 34.0 34.0 34.0 5 33.4794601498313 34.0 34.0 34.0 34.0 34.0 6 36.99435749823672 38.0 37.0 38.0 35.0 38.0 7 37.071331897291216 38.0 37.0 38.0 36.0 38.0 8 36.76564555176424 38.0 37.0 38.0 34.0 38.0 9 36.90844278388837 38.0 37.0 38.0 35.0 38.0 10-11 36.8037705636783 38.0 37.0 38.0 34.0 38.0 12-13 36.794344154482545 38.0 37.0 38.0 34.0 38.0 14-15 36.709496940467794 38.0 37.0 38.0 34.0 38.0 16-17 36.74643054575955 38.0 37.0 38.0 34.0 38.0 18-19 36.74330429478259 38.0 37.0 38.0 34.0 38.0 20-21 36.76679883337464 38.0 37.0 38.0 34.0 38.0 22-23 36.68149411921691 38.0 37.0 38.0 34.0 38.0 24-25 36.69974646867077 38.0 37.0 38.0 34.0 38.0 26-27 36.53893516841724 38.0 37.0 38.0 34.0 38.0 28-29 36.5068339083856 38.0 37.0 38.0 34.0 38.0 30-31 36.442250138203164 38.0 37.0 38.0 34.0 38.0 32-33 36.2014620942069 38.0 37.0 38.0 33.5 38.0 34-35 36.38958996549687 38.0 37.0 38.0 34.0 38.0 36-37 36.430526697039596 38.0 37.0 38.0 34.0 38.0 38-39 35.93583560494863 38.0 37.0 38.0 32.0 38.0 40-41 36.17589927371852 38.0 37.0 38.0 34.0 38.0 42-43 36.273994929373416 38.0 37.0 38.0 34.0 38.0 44-45 36.31516041098763 38.0 37.0 38.0 34.0 38.0 46-47 36.183876932461544 38.0 37.0 38.0 33.5 38.0 48-49 36.246297108217846 38.0 37.0 38.0 34.0 38.0 50-51 35.998236718198974 38.0 37.0 38.0 32.5 38.0 52-53 36.08574315179473 38.0 37.0 38.0 34.0 38.0 54-55 35.97336014792505 38.0 37.0 38.0 33.0 38.0 56-57 36.063640176137554 38.0 37.0 38.0 33.5 38.0 58-59 35.917468880459026 38.0 37.0 38.0 32.0 38.0 60-61 35.944709201471625 38.0 37.0 38.0 32.0 38.0 62-63 35.850893078404084 38.0 36.5 38.0 31.5 38.0 64-65 35.70720943975295 38.0 36.5 38.0 31.5 38.0 66-67 35.15632208010065 38.0 36.0 38.0 28.5 38.0 68-69 35.10452925141539 38.0 36.0 38.0 27.0 38.0 70-71 34.711622409882004 38.0 35.5 38.0 25.5 38.0 72-73 34.35707886158714 38.0 35.0 38.0 24.0 38.0 74-75 34.18331458853581 38.0 34.5 38.0 23.5 38.0 76 33.18913818410568 37.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02127596828908196 1101 2 -0.4409839474377222 1101 3 0.16471464071567965 1101 4 0.22440180373827445 1101 5 -0.20543235039060903 1101 6 0.061560483919379294 1101 7 0.1750448191894023 1101 8 -0.2232153640364416 1101 9 0.08997782093382511 1101 10-11 -0.046972619597426046 1101 12-13 -0.2538333663597854 1101 14-15 0.2572019017858054 1101 16-17 0.33498981216116164 1101 18-19 -0.1558165270455376 1101 20-21 0.14594861530842707 1101 22-23 -0.10729119814980237 1101 24-25 -0.023520103153323646 1101 26-27 -0.014540916382287605 1101 28-29 -0.23372926721477683 1101 30-31 -0.2720378156948371 1101 32-33 -0.6770301028253272 1101 34-35 0.19543118294049577 1101 36-37 -0.09187566176687767 1101 38-39 -0.18626670834098036 1101 40-41 -0.07680261498329344 1101 42-43 0.27110122005964143 1101 44-45 0.06920092078265583 1101 46-47 0.18576330043271128 1101 48-49 0.06239976530901714 1101 50-51 -0.22833975929479067 1101 52-53 -0.47479912296274307 1101 54-55 0.28039532500017117 1101 56-57 -0.36349064778585216 1101 58-59 -0.03628909845352979 1101 60-61 -0.26228375627683675 1101 62-63 0.13765553792916307 1101 64-65 -0.5858032021779209 1101 66-67 -0.38787919844745034 1101 68-69 -1.0607934793085576 1101 70-71 -0.6735952020084 1101 72-73 -0.08482545869174629 1101 74-75 0.10394359244483553 1101 76 -1.6807316829884726 1102 1 -0.3037717652516818 1102 2 0.13482398693749786 1102 3 0.024201239966082255 1102 4 -0.11238484501357959 1102 5 0.09076310961928868 1102 6 0.022100756884512407 1102 7 0.10772284761737438 1102 8 -0.2494511323696571 1102 9 0.002077389564050236 1102 10-11 0.15628386862830723 1102 12-13 0.061632300849041144 1102 14-15 0.19237268178721934 1102 16-17 0.13166260757874682 1102 18-19 -0.059341737426699126 1102 20-21 0.21348273595053513 1102 22-23 0.3262531056476661 1102 24-25 0.2163851369287002 1102 26-27 0.0012995475170143322 1102 28-29 0.0458832071505455 1102 30-31 0.39822949213346703 1102 32-33 0.40821846516845284 1102 34-35 0.28479897871165605 1102 36-37 0.30524971833354186 1102 38-39 -0.20032158423377666 1102 40-41 0.3908623309186936 1102 42-43 0.15901579470075689 1102 44-45 0.518868553906195 1102 46-47 0.4233172216546919 1102 48-49 0.05971254375467794 1102 50-51 0.06585796363852126 1102 52-53 0.41239060902227465 1102 54-55 0.5570200332952027 1102 56-57 0.37942003745563824 1102 58-59 -0.08398728104316433 1102 60-61 0.6092776608789521 1102 62-63 0.21006908718310768 1102 64-65 0.46338002390227473 1102 66-67 0.6297291743691744 1102 68-69 1.104364571748647 1102 70-71 0.9312241177002534 1102 72-73 0.6130212282469429 1102 74-75 0.8548452259926762 1102 76 -0.2861697708181836 1103 1 0.046691834151630474 1103 2 0.1691625168740316 1103 3 0.034818690023122656 1103 4 0.19812438310072622 1103 5 -0.0033314822084875573 1103 6 -0.4969258306143871 1103 7 0.1701405843163144 1103 8 -0.07860670872462805 1103 9 -0.248149291532215 1103 10-11 0.04705282940516042 1103 12-13 0.18943656537857123 1103 14-15 0.4624010534857206 1103 16-17 0.08267024161308711 1103 18-19 0.46120580710517345 1103 20-21 0.20165144518449551 1103 22-23 0.4283473464853671 1103 24-25 0.1214861928238733 1103 26-27 0.1920334092854148 1103 28-29 0.0984000985483604 1103 30-31 0.24872234071048638 1103 32-33 0.36511980770552555 1103 34-35 -0.12359260922598025 1103 36-37 -0.09079871829812447 1103 38-39 -0.2997102765032196 1103 40-41 -0.09487869689726836 1103 42-43 0.3225625495049371 1103 44-45 0.059773523340808765 1103 46-47 0.10479371994360775 1103 48-49 0.439893303648212 1103 50-51 0.27485793438935247 1103 52-53 -0.10540751318077213 1103 54-55 -0.17684173510983925 1103 56-57 0.17786398141421955 1103 58-59 0.37888089293092264 1103 60-61 0.5089237458770484 1103 62-63 -0.44455666963926177 1103 64-65 0.4881248248616501 1103 66-67 0.27343036481055094 1103 68-69 1.1783211074765063 1103 70-71 0.6221334745109175 1103 72-73 0.3120033570018066 1103 74-75 -0.1507121170123895 1103 76 0.30273309267592197 1104 1 0.19270975602034213 1104 2 0.23586610124777252 1104 3 0.05858026875851863 1104 4 0.1696306255493738 1104 5 0.23091136918095856 1104 6 0.20804295714236076 1104 7 0.3912692593062417 1104 8 0.34001491833940634 1104 9 0.48244374108961807 1104 10-11 0.4833949558650872 1104 12-13 0.2521001779440155 1104 14-15 0.31884990568064353 1104 16-17 0.3478610390355499 1104 18-19 0.18677719252602998 1104 20-21 -0.08941530874220405 1104 22-23 -0.19297766358310753 1104 24-25 -0.05370510802188022 1104 26-27 0.24708274476668635 1104 28-29 0.2543732157453036 1104 30-31 0.07706758839474048 1104 32-33 0.11325714157584343 1104 34-35 0.28616876814010084 1104 36-37 0.4101326147667166 1104 38-39 -0.4970018592660068 1104 40-41 -0.21631697720334842 1104 42-43 0.1267183086672361 1104 44-45 0.25727906099687203 1104 46-47 0.0842540501892799 1104 48-49 -0.21279497665786806 1104 50-51 -0.5025912198595464 1104 52-53 -0.2450948873249601 1104 54-55 -0.47377337022864197 1104 56-57 -0.5065651365881934 1104 58-59 0.17756192394180204 1104 60-61 0.05444579581664044 1104 62-63 0.1183334834014147 1104 64-65 -0.09949599672514609 1104 66-67 -0.7625140572595299 1104 68-69 -0.7745836448392396 1104 70-71 -0.4091416966529948 1104 72-73 -0.9690452736868806 1104 74-75 -0.8794424835045334 1104 76 -0.8342735525113554 1105 1 -0.338304572788104 1105 2 0.10784950999588716 1105 3 0.15888192788371214 1105 4 -0.08187767007656532 1105 5 0.029365366164370243 1105 6 0.1040648274289353 1105 7 0.2825777057617529 1105 8 -0.13802052660780362 1105 9 0.39185173505643434 1105 10-11 0.21916041589526003 1105 12-13 0.2600658642185323 1105 14-15 0.2991477940667693 1105 16-17 0.09941646889980404 1105 18-19 0.056922365980028644 1105 20-21 0.23105037753230562 1105 22-23 0.3066452625104148 1105 24-25 -0.24016815477904174 1105 26-27 -0.0633433487476367 1105 28-29 0.2910907873893436 1105 30-31 0.09945635159233035 1105 32-33 0.6094864025109885 1105 34-35 0.6546642432532224 1105 36-37 0.08236112155404385 1105 38-39 -0.17931709305183574 1105 40-41 -0.6150254990736386 1105 42-43 -0.05159051486217692 1105 44-45 -0.06045283945562119 1105 46-47 0.08387697628280222 1105 48-49 0.03494257990321614 1105 50-51 -1.080598384263773 1105 52-53 -0.030209894866437992 1105 54-55 -0.3809660549948575 1105 56-57 -0.33603346238004406 1105 58-59 0.05784095863260319 1105 60-61 0.4261935333732012 1105 62-63 0.386197359721173 1105 64-65 0.02880339995660819 1105 66-67 0.27726723987471047 1105 68-69 0.47758453012305324 1105 70-71 0.16839412536812404 1105 72-73 -0.06543479103228123 1105 74-75 0.12668496747586033 1105 76 0.288181198619867 1106 1 0.15338789367216066 1106 2 -0.1273930080639687 1106 3 -0.06206750452083298 1106 4 0.20708001826192657 1106 5 0.09923120723763645 1106 6 0.010927572526973961 1106 7 -0.10756677308241791 1106 8 0.5145594527523656 1106 9 0.18158341720297955 1106 10-11 0.393567020642422 1106 12-13 0.1261133358387525 1106 14-15 -0.13686871780114274 1106 16-17 0.1369551685902124 1106 18-19 0.3672680832142845 1106 20-21 0.27472942809990286 1106 22-23 -0.057957420668117265 1106 24-25 -0.0033002497222796023 1106 26-27 -0.06987069652885225 1106 28-29 0.19888232505704195 1106 30-31 -0.10818038731375879 1106 32-33 0.48319641283090675 1106 34-35 0.3928995373708801 1106 36-37 0.33174192650760403 1106 38-39 -0.8568196730311897 1106 40-41 0.1875544802865292 1106 42-43 0.21257357182513203 1106 44-45 0.30634282617905484 1106 46-47 0.016642714156894556 1106 48-49 0.4565370071477943 1106 50-51 0.019195217387419916 1106 52-53 0.32866523413252935 1106 54-55 0.4483167107430148 1106 56-57 0.6555300051122117 1106 58-59 -0.12719516107843987 1106 60-61 0.0084690751688683 1106 62-63 0.0756968437252965 1106 64-65 0.49144529882141086 1106 66-67 0.8467976836311593 1106 68-69 0.7003657478733132 1106 70-71 0.08916295921745387 1106 72-73 -0.07686409959780605 1106 74-75 -0.10378458876769514 1106 76 0.11000782400686404 1107 1 0.17117965568589 1107 2 0.1994948637895746 1107 3 -0.04049999880000854 1107 4 0.121553702472454 1107 5 -0.2728511726250602 1107 6 0.1902753986139274 1107 7 0.2606254465972171 1107 8 0.18721391499492768 1107 9 0.34720126617322933 1107 10-11 0.0734869291092366 1107 12-13 0.11487191707445277 1107 14-15 0.481266293640509 1107 16-17 0.5592949855238487 1107 18-19 0.005025520285229845 1107 20-21 0.21900860430127267 1107 22-23 0.07353571434332196 1107 24-25 0.32275810268046 1107 26-27 0.4375091661713739 1107 28-29 0.5312335836382758 1107 30-31 0.060240665317820685 1107 32-33 0.48491265997049027 1107 34-35 0.2238777982404372 1107 36-37 0.5558265946998233 1107 38-39 0.37172723772395955 1107 40-41 0.45205676861604616 1107 42-43 0.592750917362892 1107 44-45 0.12736685363901046 1107 46-47 0.12250655855049075 1107 48-49 0.34008963872674514 1107 50-51 0.3181654691036684 1107 52-53 0.3229444103339034 1107 54-55 0.4397926832830592 1107 56-57 0.15773252227468504 1107 58-59 0.2697441981892936 1107 60-61 -0.31501490652448894 1107 62-63 0.4192323128328539 1107 64-65 0.5094086855491042 1107 66-67 0.44505283237257487 1107 68-69 0.5496220407794965 1107 70-71 -0.05368601103425874 1107 72-73 0.1642114152763341 1107 74-75 0.4004488208432946 1107 76 0.27379500936155665 1108 1 -0.10579899570697648 1108 2 0.22728710539333008 1108 3 0.2815190066673168 1108 4 0.10733214558622706 1108 5 -0.01718858475732077 1108 6 -0.11482093046704023 1108 7 0.04831094516529788 1108 8 0.3635351854973976 1108 9 0.21042047991964807 1108 10-11 0.11055409911705283 1108 12-13 -0.17435940289431073 1108 14-15 -0.11047413853861343 1108 16-17 -0.1296694768557387 1108 18-19 -0.3672733602146394 1108 20-21 0.37372091880270375 1108 22-23 0.17355849486922637 1108 24-25 0.39977255204261297 1108 26-27 0.05560992118309116 1108 28-29 -0.05310317762180716 1108 30-31 -0.14035813707738498 1108 32-33 0.21499597160809003 1108 34-35 0.2518815732990163 1108 36-37 -0.0022637463571868466 1108 38-39 -0.1824578703208246 1108 40-41 -0.39191290074089125 1108 42-43 -0.15677720031480646 1108 44-45 -0.09677724945914434 1108 46-47 0.4373171547493939 1108 48-49 0.2177093992057877 1108 50-51 0.1142472186480532 1108 52-53 -0.3007863889371194 1108 54-55 0.05502960075246932 1108 56-57 0.0829961255030085 1108 58-59 0.07824133435174474 1108 60-61 0.3318381265398145 1108 62-63 -0.04422121489430708 1108 64-65 0.1476539341822729 1108 66-67 0.3036996691338345 1108 68-69 0.7263663781916279 1108 70-71 1.548208026976667 1108 72-73 0.7866404702099388 1108 74-75 0.9540156003278071 1108 76 1.064246714646636 1109 1 -0.10137646821294055 1109 2 0.155235130910917 1109 3 0.21443748343926217 1109 4 0.33969095737441535 1109 5 0.27446340538005387 1109 6 0.33772495814788783 1109 7 0.13715286916044533 1109 8 0.37014940803272367 1109 9 0.4228974866904238 1109 10-11 0.26818002373839533 1109 12-13 0.5280397204276781 1109 14-15 0.3890170751125197 1109 16-17 0.5267040505991503 1109 18-19 0.3616609280715295 1109 20-21 0.08480854746694888 1109 22-23 -0.13779918193403518 1109 24-25 0.32764230273729567 1109 26-27 0.35577429831118934 1109 28-29 0.14076399000372675 1109 30-31 0.5635086391740316 1109 32-33 0.2737020887854982 1109 34-35 0.2592394066519148 1109 36-37 0.6134068731880333 1109 38-39 0.2732972860331344 1109 40-41 0.13170652707015051 1109 42-43 0.06360556356919744 1109 44-45 0.5187955265574473 1109 46-47 0.6247266494854173 1109 48-49 0.42138048839994724 1109 50-51 0.20371534122642743 1109 52-53 0.37828683715971323 1109 54-55 -0.10073729682492427 1109 56-57 0.16641751801211058 1109 58-59 0.5039193900051444 1109 60-61 0.19988634383071968 1109 62-63 -0.014946431128500137 1109 64-65 0.491221700610204 1109 66-67 0.5133783509858176 1109 68-69 0.2218655758746877 1109 70-71 1.1773810534727005 1109 72-73 -0.2901079936466573 1109 74-75 0.305606536103312 1109 76 0.18197204829306912 1110 1 0.10110719191777662 1110 2 -0.01862107823940562 1110 3 -0.036804346626091444 1110 4 0.10988703580579084 1110 5 0.32975752302711214 1110 6 -0.01573909413968977 1110 7 0.2612051567421432 1110 8 0.5931559439804346 1110 9 -0.050697284551411315 1110 10-11 -0.010643505673371578 1110 12-13 0.35085017794401097 1110 14-15 -0.38967573534500133 1110 16-17 0.39186744929196493 1110 18-19 0.12604001303884615 1110 20-21 0.433501357924456 1110 22-23 0.12958643898099353 1110 24-25 0.48091027659351226 1110 26-27 0.397146847330788 1110 28-29 0.38344372856581543 1110 30-31 -0.06309266801551416 1110 32-33 0.1572315005502034 1110 34-35 0.2728995373708756 1110 36-37 0.26647876861287045 1110 38-39 -0.045591602855751034 1110 40-41 0.7294843048479294 1110 42-43 -0.2028650246660959 1110 44-45 0.38827265074046124 1110 46-47 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0.19959873103933035 2116 8 -0.27415329296735536 2116 9 0.3195035186614419 2116 10-11 0.04670589191698582 2116 12-13 -0.08637873771863269 2116 14-15 -0.08598095622853208 2116 16-17 -0.9362851611497973 2116 18-19 -0.1665302680856513 2116 20-21 -0.6108761521156936 2116 22-23 -0.2641083401354649 2116 24-25 -0.4461981571414313 2116 26-27 -0.4979937149182092 2116 28-29 0.18147585707987446 2116 30-31 0.30120452073951043 2116 32-33 0.014902183281130021 2116 34-35 -0.5583052819062289 2116 36-37 -0.4518344843991784 2116 38-39 -0.4127803578758318 2116 40-41 -1.0041703136259628 2116 42-43 -0.7012184383207156 2116 44-45 -1.246225341227408 2116 46-47 -0.40555132515826386 2116 48-49 -0.03566337332144798 2116 50-51 -0.540792100304401 2116 52-53 -0.10934998463728363 2116 54-55 -0.6075488567902738 2116 56-57 -0.8377166657818904 2116 58-59 -0.08112667661427508 2116 60-61 -0.6095080966764073 2116 62-63 0.538601092298542 2116 64-65 -0.3596326973738897 2116 66-67 0.30351930696660645 2116 68-69 -0.06437743538603513 2116 70-71 0.4347241513544233 2116 72-73 -0.6646256722040249 2116 74-75 -0.398267784709347 2116 76 0.6916184771457381 2117 1 0.3420080928186735 2117 2 0.23092847131014338 2117 3 0.11887132904958264 2117 4 0.13168883760759087 2117 5 0.05029806356765221 2117 6 0.03795459955399849 2117 7 0.05616011169709623 2117 8 -0.9963936055691178 2117 9 -0.15412070797483324 2117 10-11 -0.3514543164841797 2117 12-13 0.07220152929536994 2117 14-15 0.23581976015049833 2117 16-17 0.31583141325592123 2117 18-19 -0.420356383357543 2117 20-21 -0.04127341685031638 2117 22-23 -0.36293608354152695 2117 24-25 -0.2501708044875741 2117 26-27 -0.1610513508674032 2117 28-29 -0.7702499651278814 2117 30-31 -0.020840415763260012 2117 32-33 -0.7248405715218667 2117 34-35 -0.17601938154804486 2117 36-37 -0.5491969070628073 2117 38-39 0.26729128002713054 2117 40-41 -0.2436688483052194 2117 42-43 0.4637115519104782 2117 44-45 -0.11947509700728887 2117 46-47 -0.5248935589583255 2117 48-49 -0.4386265774894724 2117 50-51 0.627801191782865 2117 52-53 -0.15355382703389608 2117 54-55 0.29981030960503574 2117 56-57 -0.05165349417654852 2117 58-59 0.05360142498130216 2117 60-61 -0.06009422497337624 2117 62-63 -0.09002150620357696 2117 64-65 -0.37925171398085666 2117 66-67 -0.03798183272731137 2117 68-69 -0.6723227300783208 2117 70-71 -1.4850902413515357 2117 72-73 -1.1883150242066307 2117 74-75 -1.1209965375585895 2117 76 -1.0929793737171742 2118 1 0.14540826718658906 2118 2 -0.17883613200284998 2118 3 -0.05680434662609457 2118 4 -0.6808656523662577 2118 5 -0.21604892858579205 2118 6 0.059314669301166134 2118 7 0.24099010297869938 2118 8 0.25208067516322785 2118 9 -0.7683316931535558 2118 10-11 -0.2664499572862695 2118 12-13 -0.09366595108824072 2118 14-15 -0.3295682084632787 2118 16-17 0.07498572886186139 2118 18-19 -0.6422395568536245 2118 20-21 -0.933165308742204 2118 22-23 -0.10116624919103856 2118 24-25 -0.790057465341981 2118 26-27 -0.021992937615443964 2118 28-29 0.0954867393185097 2118 30-31 0.3424987298339488 2118 32-33 -0.4887900048261429 2118 34-35 -0.20064884972589425 2118 36-37 -1.0267470378387458 2118 38-39 -0.682795903931023 2118 40-41 -0.015246877947767246 2118 42-43 -0.9730800784295397 2118 44-45 0.06644469375121531 2118 46-47 -1.1545796967089785 2118 48-49 -1.0933781032087424 2118 50-51 0.0604515841106803 2118 52-53 -0.5147133736773171 2118 54-55 0.6559567786547262 2118 56-57 -0.13513213410681146 2118 58-59 -0.6521527162567153 2118 60-61 -0.38049527118087667 2118 62-63 -0.5104661443901506 2118 64-65 -0.5701845823330842 2118 66-67 0.03525269212012461 2118 68-69 -0.13919282598067184 2118 70-71 0.24943460607653378 2118 72-73 -0.5693089213295579 2118 74-75 -0.6556521609238928 2118 76 -1.5149497311714022 2119 1 -0.209686458875872 2119 2 0.13375987414154622 2119 3 -0.41394720376894867 2119 4 -0.3447161387973878 2119 5 -0.7600837468141606 2119 6 -0.13192957033017194 2119 7 -0.4464138908769044 2119 8 -0.4785900877655962 2119 9 -0.02434807820220186 2119 10-11 -0.7112784263082972 2119 12-13 -0.1839117268178896 2119 14-15 -1.3350725607418212 2119 16-17 -1.5727357253112118 2119 18-19 -0.5795155425167025 2119 20-21 0.023183897606998016 2119 22-23 -0.3166040372094727 2119 24-25 -0.8913119456287149 2119 26-27 -0.3001547399707931 2119 28-29 -0.18369912857703952 2119 30-31 -0.7572196521424956 2119 32-33 -1.4741970708783612 2119 34-35 -1.582497288025948 2119 36-37 -0.7862196440855413 2119 38-39 -0.4886074758716248 2119 40-41 -1.1619442665806403 2119 42-43 -0.9303253421264088 2119 44-45 -0.43125115878334697 2119 46-47 -1.0115945456593138 2119 48-49 -1.3097119485748365 2119 50-51 0.03331384729552411 2119 52-53 -0.38081680429687736 2119 54-55 0.2732122830070125 2119 56-57 -0.5389979815210353 2119 58-59 -0.24444662306675013 2119 60-61 -0.420561835440985 2119 62-63 -0.34827976446183584 2119 64-65 -0.3411779259070684 2119 66-67 -0.18223322697869548 2119 68-69 -0.4942875520431471 2119 70-71 0.09288058661928744 2119 72-73 1.2285661426745378 2119 74-75 0.7743273577653085 2119 76 -0.7461325268703263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 4.0 23 9.0 24 17.0 25 47.0 26 66.0 27 176.0 28 260.0 29 459.0 30 729.0 31 1190.0 32 1941.0 33 3208.0 34 4858.0 35 6589.0 36 11160.0 37 21742.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.509731409291064 17.463161707238033 11.3993785623058 30.627728321165097 2 23.46594483310776 22.80828837759012 29.930040603137687 23.795726186164433 3 20.631350197296936 21.123162850988393 29.531634228635696 28.713852723078976 4 15.219504756095237 21.84753807735565 33.931260603518936 29.001696563030176 5 14.426504508282658 32.04026001258126 37.25385539182981 16.27938008730628 6 28.405040126575038 35.833698698030844 17.838693074591585 17.922568100802533 7 28.16866505270783 27.77025867820584 23.549819859318706 20.51125640976763 8 24.804132751291487 34.35444823576507 17.798661812081818 23.042757200861626 9 23.900569968928114 17.48413046379077 18.767037114699097 39.84826245258202 10-11 26.353914485598278 26.654148954421547 24.522960788425248 22.468975771554927 12-13 26.364398863874644 21.247069139709108 25.36552355172611 27.023008444690138 14-15 22.70153834423073 21.66835052135954 22.018147505671095 33.611963628738636 16-17 25.065289082902837 27.287977277492896 22.558569549552985 25.08816409005128 18-19 26.02889875903086 26.915305286032904 23.52885110276597 23.526944852170267 20-21 24.216054442517013 26.02699250843516 23.324882289025712 26.432070760022114 22-23 22.915038410949503 27.48813359004175 23.84052307516346 25.756304923845292 24-25 24.29039821574944 26.31483634838636 26.794258373205743 22.600507062658455 26-27 25.69244552888923 27.06303970719991 23.826226195695686 23.41828856821518 28-29 23.96156998799062 26.450180140681294 24.93375779179931 24.654492079528776 30-31 23.63846051201891 26.06130501915782 27.72832116510036 22.57191330372291 32-33 23.85005432814198 24.42764825864008 26.535008292190092 25.18728912102785 34-35 24.5830076821899 23.856726205226938 25.612383003869688 25.94788310871347 36-37 26.986789683371775 25.253054766579613 25.309289159152865 22.450866390895747 38-39 22.447053889704343 27.03921157475362 27.40330543853295 23.110429097009096 40-41 23.756648048952513 23.42496044530014 27.031586572370802 25.78680493337654 42-43 25.437007949064984 23.62035113135973 26.40728950227797 24.535351417297317 44-45 21.907584971120304 25.4741798356812 28.713852723078976 23.904382470119522 46-47 23.552679235212263 23.708991784059933 28.643321451037952 24.095007529689852 48-49 23.046569702053034 23.28199165062239 26.93722716788349 26.73421147944109 50-51 23.439257324767915 23.62511675784899 27.257477267961644 25.678148649421452 52-53 20.924912789035247 23.467851083703465 32.48632265197583 23.120913475285462 54-55 22.00385062620332 24.833679635524884 28.44602451438266 24.71644522388913 56-57 22.45849139327856 26.473055147829733 26.158523799538685 24.90992965935302 58-59 20.625631445509825 26.726586477058273 28.43172763491489 24.216054442517013 60-61 21.834194323185727 28.50702453344517 26.377742618044568 23.28103852532454 62-63 19.954631235822262 31.350197296936656 25.155835986198742 23.539335481042336 64-65 20.19577193617873 31.994509998284375 24.267523208601002 23.542194856935893 66-67 20.20244381326369 30.911759659924893 24.0921481537963 24.793648373015117 68-69 19.404677938961857 30.946072170647554 24.875617148630358 24.77363274176023 70-71 20.135725042414077 29.761337425417945 24.57633580510494 25.526601727063042 72-73 20.175756304923844 28.51274328523228 24.62399206999752 26.687508339846357 74-75 20.25200632875198 28.60043081263463 24.62971082178463 26.51785203682876 76 20.671381459806707 28.725290226653193 24.054976267180084 26.548352046360012 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.5 16 2.5 17 4.0 18 2.5 19 5.5 20 13.0 21 16.0 22 14.5 23 23.0 24 36.0 25 39.0 26 81.0 27 141.5 28 189.5 29 219.0 30 280.5 31 408.0 32 626.0 33 778.0 34 824.0 35 996.5 36 1266.5 37 1410.0 38 1540.0 39 1941.0 40 2454.5 41 3083.5 42 3470.0 43 3731.0 44 4048.0 45 3871.0 46 3638.0 47 3524.5 48 3235.0 49 2778.0 50 2497.0 51 2313.0 52 1849.0 53 1465.0 54 1361.0 55 1280.0 56 1063.0 57 878.0 58 829.0 59 750.0 60 696.5 61 720.5 62 719.0 63 695.5 64 694.5 65 750.5 66 805.5 67 827.0 68 794.0 69 735.5 70 638.5 71 567.0 72 484.0 73 353.0 74 214.5 75 124.0 76 96.0 77 46.0 78 14.0 79 4.0 80 3.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.621704569282684 #Duplication Level Percentage of deduplicated Percentage of total 1 69.59586794164863 38.01444937951543 2 16.05709499546311 17.54131798166187 3 6.187617784602499 10.139346918545913 4 2.9315278844140433 6.405002001563126 5 1.682138619389963 4.5940639356449795 6 1.0783834717665945 3.534188604433939 7 0.7468416276959586 2.8555633923635604 8 0.4955678090318978 2.1655006767189615 9 0.3524813289593076 1.73278179149431 >10 0.8375793955468694 6.313501972969367 >50 0.013959656592447826 0.5127814102441907 >100 0.013959656592447826 1.2524066413770754 >500 0.0 0.0 >1k 0.006979828296223913 4.939095293467279 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG 1506 2.8708133971291865 RNA PCR Primer, Index 18 (95% over 24bp) CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 1085 2.0682818963380925 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 259 0.4937189042871576 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 156 0.29737509292971653 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 134 0.2554375798242437 RNA PCR Primer, Index 30 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT 108 0.2058750643359576 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT 91 0.17346880420900132 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 66 0.12581253931641853 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 60 0.11437503574219868 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.001906250595703311 0.0 5 0.0 0.0 0.0 0.001906250595703311 0.0 6 0.0 0.0 0.0 0.001906250595703311 0.0 7 0.0 0.0 0.0 0.001906250595703311 0.0 8 0.0 0.0 0.0 0.001906250595703311 0.0 9 0.0 0.0 0.0 0.001906250595703311 0.0 10 0.0 0.0 0.0 0.001906250595703311 0.0 11 0.0 0.0 0.0 0.001906250595703311 0.0 12 0.0 0.0 0.0 0.001906250595703311 0.0 13 0.0 0.0 0.0 0.001906250595703311 0.0 14 0.0 0.0 0.0 0.001906250595703311 0.0 15 0.0 0.0 0.0 0.001906250595703311 0.0 16 0.0 0.0 0.0 0.001906250595703311 0.0 17 0.0 0.0 0.0 0.001906250595703311 0.0 18 0.0 0.0 0.0 0.001906250595703311 0.0 19 0.0 0.0 0.0 0.007625002382813244 0.0 20 0.0 0.0 0.0 0.007625002382813244 0.0 21 0.0 0.0 0.0 0.007625002382813244 0.0 22 0.0 0.0 0.0 0.007625002382813244 0.0 23 0.0 0.0 0.0 0.007625002382813244 0.0 24 0.0 0.0 0.0 0.007625002382813244 0.0 25 0.0 0.0 0.0 0.007625002382813244 0.0 26 0.0 0.0 0.0 0.007625002382813244 0.0 27 0.0 0.0 0.0 0.022875007148439735 0.0 28 0.0 0.0 0.0 0.0343125107226596 0.0 29 0.0 0.0 0.0 0.05337501667969271 0.0 30 0.0 0.0 0.0 0.06671877084961589 0.0 31 0.0 0.0 0.0 0.07625002382813245 0.0 32 0.0 0.0 0.0 0.1029375321679788 0.0 33 0.0 0.0 0.0 0.12200003812501191 0.0 34 0.0 0.0 0.0 0.15059379706056158 0.0 35 0.0 0.0 0.0 0.20968756552736423 0.0 36 0.0 0.0 0.0 0.30690634590823307 0.0 37 0.0 0.0 0.0 0.45178139118168475 0.0 38 0.0 0.0 0.0 0.655750204921939 0.0 39 0.0 0.0 0.0 0.8540002668750833 0.0 40 0.0 0.0 0.0 1.1170628490821404 0.0 41 0.0 0.0 0.0 1.5364379801368688 0.0 42 0.0 0.0 0.0 2.047313139785356 0.0 43 0.0 0.0 0.0 2.55628204883814 0.0 44 0.0 0.0 0.0 3.038563449551078 0.0 45 0.0 0.0 0.0 3.557063611582379 0.0 46 0.0 0.0 0.0 4.033626260508206 0.0 47 0.0 0.0 0.0 4.508282658838331 0.0 48 0.0 0.0 0.0 5.013439066699708 0.0 49 0.0 0.0 0.0 5.432814197754436 0.0 50 0.0 0.0 0.0 5.861720581787682 0.0 51 0.0 0.0 0.0 6.4145332545416425 0.0 52 0.0 0.0 0.0 7.076002211250691 0.0 53 0.0 0.0 0.0 7.846127451914828 0.0 54 0.0 0.0 0.0 8.57240892887779 0.0 55 0.0 0.0 0.0 9.151909109971596 0.0 56 0.0 0.0 0.0 9.676128023790007 0.0 57 0.0 0.0 0.0 10.166034426885759 0.0 58 0.0 0.0 0.0 10.724565851426828 0.0 59 0.0 0.0 0.0 11.130597228311634 0.0 60 0.0 0.0 0.0 11.58237861949332 0.0 61 0.0 0.0 0.0 12.133285041651575 0.0 62 0.0 0.0 0.0 12.726128976915305 0.0 63 0.0 0.0 0.0 13.378066680645837 0.0 64 0.0 0.0 0.0 14.010941878419336 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATATA 20 7.858393E-5 70.00001 2 CTCCGCC 15 0.0022130527 70.0 9 TCACAAG 15 0.0022130527 70.0 55 GCGTGAC 15 0.0022130527 70.0 11 TCTTTAT 35 2.9211515E-7 60.0 1 CTTTATA 35 2.9211515E-7 60.0 2 TTTAGAC 25 2.3699876E-4 55.999996 42 CTTAATA 20 0.0069152876 52.500004 1 CGGAACT 20 0.0069152876 52.500004 34 TTAATAC 20 0.0069152876 52.500004 2 CTTTTGG 20 0.0069152876 52.500004 31 TTAGACG 20 0.0069152876 52.500004 43 GCCGGAA 20 0.0069152876 52.500004 32 TCGGAAT 20 0.0069152876 52.500004 68 CGAGGGT 20 0.0069152876 52.500004 28 CAATGAC 20 0.0069152876 52.500004 55 AACTCGT 20 0.0069152876 52.500004 37 TTCCTTC 20 0.0069152876 52.500004 25 TTTATAC 50 6.050686E-8 48.999996 3 ATATACA 45 1.6549966E-6 46.666668 4 >>END_MODULE