FastQCFastQC Report
Wed 25 May 2016
SRR1780339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579141
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT502.3808188E-630.7630735
CTACATT407.0379267E-427.49314125
CATCGTT508.387551E-526.37518132
CAAAGGT609.6951135E-625.68245713
GGTAGCA701.1169068E-625.17792717
GTATCCA450.001386030624.4785418
AGTCATC852.3756002E-723.266198
TAGCATA703.1772375E-522.03068719
CAGTGTA703.192133E-522.01734534
CAGAGTT803.5855446E-622.01734534
TTCAATG500.002596854721.9736238
GACCATA500.002596854721.9736236
TAGATTG500.002596854721.9736235
TCAAGAC500.002596854721.9736233
CACTTGT755.476825E-520.52821228
ATGTATC550.004444827320.02789916
AAAAAGC1101.2443525E-720.02789915
ATGGTTC550.004487308719.9950126
GCAGAGT1001.131526E-619.78138533
TAGATCT1352.564775E-919.57097439
Produced by FastQC (version 0.11.5)