FastQCFastQC Report
Wed 25 May 2016
SRR1780339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579141
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT189543.2727781317502993No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG49930.8621389264445102No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC49270.8507427379515524No Hit
CACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTG32940.5687734075121602TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC10780.18613774538497532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCT3950.035.0897143
ATCGTAT451.0357217E-634.22329739
ATATCGT451.0357217E-634.22329737
ATGCCGT26100.033.8861244
TCTTCTG4150.033.3986444
CCGTCTT4400.031.00097541
CGTCTTC4700.029.49028842
TGTTTAC300.00574169129.33425543
GCGAAAA300.00574169129.33425516
GCGCTAT300.005744115429.33172419
ACGGTTT300.005744115429.33172412
TTGTACG300.005746539729.329199
TATGCCG30550.029.02218643
TCTTTAT1550.028.4592591
TATCGTA554.9539813E-628.0008838
GCCGTCT5250.026.4008340
CTTTATA1700.025.880932
TTAATAC609.745683E-625.6652583
GTATGCC36550.024.25794242
TCGTATG36900.024.08747740
Produced by FastQC (version 0.11.5)