FastQCFastQC Report
Wed 25 May 2016
SRR1780338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85653
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGA200.006930200452.519
TTAGATT200.006930200452.538
CACATGG200.006930200452.534
GATTTAC200.006930200452.541
AAAGTGT200.006930200452.518
ATTCCGG200.006930200452.51
CATAGTC200.006930200452.551
TGGATTC200.006930200452.524
AGTTAGA200.006930200452.536
AAGGGGG200.006930200452.566
GCAGAGG200.006930200452.58
TGGGTTC350.001249274540.038
GAGAATT350.001249274540.016
AGGTAAT400.002406996235.045
CATTCTT400.002406996235.014
TAACATA400.002406996235.05
GTGTATT400.002406996235.01
GCGAAGA450.00428644831.11111326
GGTCATT450.00428644831.11111351
AGTGGAA450.00428644831.11111320
Produced by FastQC (version 0.11.5)