##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780338_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85653 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.770060593324228 34.0 34.0 34.0 23.0 34.0 2 31.905082133725614 34.0 34.0 34.0 24.0 34.0 3 31.74651208947731 34.0 34.0 34.0 23.0 34.0 4 31.61843718258555 34.0 34.0 34.0 23.0 34.0 5 31.6712082472301 34.0 34.0 34.0 23.0 34.0 6 34.961460777789455 38.0 37.0 38.0 27.0 38.0 7 34.915811471869056 38.0 36.0 38.0 26.0 38.0 8 34.99764164711102 38.0 37.0 38.0 27.0 38.0 9 35.26149697033379 38.0 37.0 38.0 27.0 38.0 10-11 35.16293066209006 38.0 37.0 38.0 27.0 38.0 12-13 35.18291244906775 38.0 37.0 38.0 27.0 38.0 14-15 35.18767585490292 38.0 37.0 38.0 27.0 38.0 16-17 35.23741141582899 38.0 37.0 38.0 27.0 38.0 18-19 35.240265956825795 38.0 37.0 38.0 27.0 38.0 20-21 35.27424608595146 38.0 37.0 38.0 27.0 38.0 22-23 35.241480158313195 38.0 37.0 38.0 27.0 38.0 24-25 35.310117567394016 38.0 37.0 38.0 27.0 38.0 26-27 35.33626376192311 38.0 37.0 38.0 27.0 38.0 28-29 35.39097871644893 38.0 37.0 38.0 28.5 38.0 30-31 35.39308605653042 38.0 37.0 38.0 27.0 38.0 32-33 35.44279242992073 38.0 37.0 38.0 29.5 38.0 34-35 35.42039391498255 38.0 37.0 38.0 28.5 38.0 36-37 35.39023735304076 38.0 37.0 38.0 27.0 38.0 38-39 35.3803311034056 38.0 37.0 38.0 27.0 38.0 40-41 35.4223144548352 38.0 37.0 38.0 29.0 38.0 42-43 35.43940667577318 38.0 37.0 38.0 28.5 38.0 44-45 35.47582104538078 38.0 37.0 38.0 30.5 38.0 46-47 35.41148004156305 38.0 37.0 38.0 29.5 38.0 48-49 35.39028989060512 38.0 37.0 38.0 27.5 38.0 50-51 35.36231655633778 38.0 37.0 38.0 27.0 38.0 52-53 35.387394487058245 38.0 37.0 38.0 27.0 38.0 54-55 35.379134414439655 38.0 37.0 38.0 27.0 38.0 56-57 35.303632096949315 38.0 37.0 38.0 27.0 38.0 58-59 35.352737207103075 38.0 37.0 38.0 27.0 38.0 60-61 35.377488237423094 38.0 37.0 38.0 28.0 38.0 62-63 35.26826264112174 38.0 37.0 38.0 27.0 38.0 64-65 35.264398211387814 38.0 37.0 38.0 27.0 38.0 66-67 35.200384107970535 38.0 37.0 38.0 27.0 38.0 68-69 35.156579454309835 38.0 37.0 38.0 27.0 38.0 70-71 35.13413423931444 38.0 37.0 38.0 27.0 38.0 72-73 35.19837016800345 38.0 37.0 38.0 27.0 38.0 74-75 35.190758058678625 38.0 37.0 38.0 27.0 38.0 76 33.87960725251889 37.0 34.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5533418529504353 1101 2 -0.6195263208178332 1101 3 -0.35644436849045036 1101 4 -0.4594138133508707 1101 5 -0.7153194044340765 1101 6 -0.5659982496202218 1101 7 -1.1699413533920335 1101 8 -0.7738035334544406 1101 9 -1.126538934692178 1101 10-11 -1.093443400920215 1101 12-13 -0.8914445597211866 1101 14-15 -0.8935691073095953 1101 16-17 -1.1476837146296717 1101 18-19 -0.8519774539676845 1101 20-21 -1.0913137810291431 1101 22-23 -0.7106418450922547 1101 24-25 -0.8276507901973247 1101 26-27 -1.4670514088841458 1101 28-29 -0.995122744388226 1101 30-31 -1.028984037294947 1101 32-33 -1.1029342858733528 1101 34-35 -0.945477455417084 1101 36-37 -0.8075392669662307 1101 38-39 -1.17225373274848 1101 40-41 -0.7193589259677253 1101 42-43 -1.2739198063504844 1101 44-45 -0.6900474819021483 1101 46-47 -0.8094337471433306 1101 48-49 -0.6965346969770891 1101 50-51 -0.7392182289875464 1101 52-53 -0.8914283100119036 1101 54-55 -0.9409931375356919 1101 56-57 -1.0284921324106193 1101 58-59 -1.177379088952371 1101 60-61 -1.1923257104646936 1101 62-63 -1.0046405204126074 1101 64-65 -1.046223160165269 1101 66-67 -0.9912680744802316 1101 68-69 -1.0695128181564328 1101 70-71 -1.2576802689825044 1101 72-73 -0.7463769884254958 1101 74-75 -1.3172896224460473 1101 76 -1.8667193075247326 1102 1 -0.2516558677028051 1102 2 -0.30956847044902247 1102 3 -0.4820502694388189 1102 4 -0.15651602620650706 1102 5 -0.3073636615468267 1102 6 -0.17226800725984504 1102 7 -0.7499202785764396 1102 8 -0.13107434483484326 1102 9 -0.28807739623064066 1102 10-11 -0.28364361166836716 1102 12-13 -0.2237680581405712 1102 14-15 -0.52104539814205 1102 16-17 -0.5598017335970056 1102 18-19 -0.695734039847558 1102 20-21 -0.8257184174885737 1102 22-23 -1.038302540561176 1102 24-25 -0.6039679661720356 1102 26-27 -0.7779049389157677 1102 28-29 -0.6066348624071907 1102 30-31 -0.5369661237016956 1102 32-33 -0.8412114196984319 1102 34-35 -0.6967946313917963 1102 36-37 -0.8351736189156469 1102 38-39 -0.6501251763733435 1102 40-41 -0.7701228880330504 1102 42-43 -0.6793202278467945 1102 44-45 -0.6367018549263932 1102 46-47 -1.0834590369220436 1102 48-49 -0.6647117254280985 1102 50-51 -0.6233857737715383 1102 52-53 -0.2628455913606942 1102 54-55 -0.9408614199382228 1102 56-57 -0.734445767816311 1102 58-59 -0.7633379930619597 1102 60-61 -0.7663773437629047 1102 62-63 -0.7086710788952217 1102 64-65 -0.7029671011557852 1102 66-67 -0.521326030223122 1102 68-69 -0.6330007001374653 1102 70-71 -0.5114473922701777 1102 72-73 -0.48863199369420585 1102 74-75 -0.7684223937842987 1102 76 -0.19585524008532218 1103 1 -0.24475995865425304 1103 2 -0.23865243280108928 1103 3 -0.24579549355586394 1103 4 0.22821046713055182 1103 5 0.019819266418881654 1103 6 -0.6575210125715572 1103 7 0.3336693216472071 1103 8 -0.3181258777767866 1103 9 -0.11120664793131851 1103 10-11 -0.22995569814679584 1103 12-13 -0.0993854283763369 1103 14-15 -0.3861907713598285 1103 16-17 -0.5608966921283667 1103 18-19 -0.32177075590384874 1103 20-21 -0.2525775172928775 1103 22-23 -0.2743126980488313 1103 24-25 -0.3827397488577162 1103 26-27 -0.701549054096084 1103 28-29 -0.3941546648487133 1103 30-31 -0.6208469363007083 1103 32-33 -0.2126935742040672 1103 34-35 -0.557199067138697 1103 36-37 -0.6146559597972043 1103 38-39 -0.8453306558253999 1103 40-41 -0.5332390934193114 1103 42-43 -0.609359366790045 1103 44-45 -0.22229499629252558 1103 46-47 -0.10434478848294049 1103 48-49 -0.36363307478975315 1103 50-51 -0.97210676901895 1103 52-53 -0.3026135533400023 1103 54-55 -0.5510794797648941 1103 56-57 -0.4811608922222348 1103 58-59 -0.44251514337413056 1103 60-61 -0.4215120003652686 1103 62-63 -0.6475630740494509 1103 64-65 -0.2594623019758373 1103 66-67 -0.38810219274292024 1103 68-69 -0.20942647592723063 1103 70-71 -0.5058095206831794 1103 72-73 -0.39445391150242415 1103 74-75 -0.38381238539737694 1103 76 -0.32650476016733165 1104 1 -0.23300674251631293 1104 2 -0.26114855996482333 1104 3 -0.17834078890172478 1104 4 0.6425029167836804 1104 5 -0.30883298656151936 1104 6 -0.136472989401895 1104 7 0.17636481568641216 1104 8 -0.04184110644251149 1104 9 -0.32108069656067073 1104 10-11 -0.18112428439317085 1104 12-13 -0.2786303936891912 1104 14-15 0.28443292366273454 1104 16-17 0.32544398275545205 1104 18-19 0.2554165546639453 1104 20-21 -0.15733942205478257 1104 22-23 0.2665541437373946 1104 24-25 -0.16510318928338563 1104 26-27 0.06604709327375247 1104 28-29 0.23356473974480707 1104 30-31 -0.0027591071096253472 1104 32-33 0.0030570824942515173 1104 34-35 0.32127806628892586 1104 36-37 0.19643382265042675 1104 38-39 0.4302469019303743 1104 40-41 -0.05711597090298426 1104 42-43 0.3276986247233893 1104 44-45 0.4139446480800757 1104 46-47 0.20359620969851022 1104 48-49 0.2423647698926672 1104 50-51 -0.028936431577044175 1104 52-53 0.09835970725136178 1104 54-55 0.1367867558382585 1104 56-57 0.1791240138193828 1104 58-59 0.05026117507403427 1104 60-61 -0.0670464517695919 1104 62-63 0.2544036532355207 1104 64-65 0.17293144760320445 1104 66-67 0.06340316187417727 1104 68-69 0.5969317109118606 1104 70-71 0.14075207425182157 1104 72-73 0.4611566495536863 1104 74-75 0.37650956516502987 1104 76 0.2174771890479974 1105 1 -0.48181688668862677 1105 2 -0.11298111164104441 1105 3 -0.16312453043377317 1105 4 0.22634866978003387 1105 5 0.0464214862541823 1105 6 -0.007968560011583747 1105 7 0.20617200693185112 1105 8 0.0758590846292293 1105 9 -0.38281423833590367 1105 10-11 0.08479546547655303 1105 12-13 -0.428117974026172 1105 14-15 -0.3605731558925882 1105 16-17 -0.1523395850750191 1105 18-19 -0.2897979667881927 1105 20-21 -0.339795562405655 1105 22-23 -0.09319920001803439 1105 24-25 -0.2474551086858554 1105 26-27 0.019217147121246114 1105 28-29 -0.2334493300832321 1105 30-31 -0.3376210143529832 1105 32-33 -0.05259690395702421 1105 34-35 0.1246305067961373 1105 36-37 -0.05591983376784526 1105 38-39 0.34765180031495646 1105 40-41 -0.22715244823493208 1105 42-43 0.099663189600939 1105 44-45 0.10829934670783103 1105 46-47 -0.1212083760232261 1105 48-49 -0.08509745001352798 1105 50-51 0.27427704766562044 1105 52-53 -0.035928984775736694 1105 54-55 -0.161337267090353 1105 56-57 -0.055077827417370884 1105 58-59 0.059901612802015336 1105 60-61 0.25777550411020655 1105 62-63 0.195258265012491 1105 64-65 -0.08934056907214938 1105 66-67 0.13713948287468725 1105 68-69 0.05609446929296524 1105 70-71 -0.2826802689824959 1105 72-73 -0.004103034309437703 1105 74-75 -0.21145291529354182 1105 76 -0.5925627623155592 1106 1 -0.44884159130731405 1106 2 -0.74885651443374 1106 3 -0.43627168403205374 1106 4 -0.3806330702844143 1106 5 -0.5039902573906438 1106 6 -0.37520808740148937 1106 7 -1.3125891817541486 1106 8 -0.6276496873005968 1106 9 -0.30848555949594925 1106 10-11 -0.4374386913440773 1106 12-13 -0.03135821749198442 1106 14-15 -0.21612274414894017 1106 16-17 -0.3142980526725836 1106 18-19 -0.20102245658411988 1106 20-21 -0.1437339798779078 1106 22-23 0.013102267937568968 1106 24-25 -0.04260369443593959 1106 26-27 -0.01957626498043652 1106 28-29 -0.3159233723317101 1106 30-31 -0.24669727643676254 1106 32-33 -0.2637139823673351 1106 34-35 -0.2544779787033207 1106 36-37 -0.06703691217816043 1106 38-39 -0.4694803156893457 1106 40-41 -0.3359601818546878 1106 42-43 -0.14677433277643814 1106 44-45 -0.2376011187415017 1106 46-47 -0.2710114551496119 1106 48-49 -0.11873511560608563 1106 50-51 -0.2544196941890178 1106 52-53 -0.14785164857809718 1106 54-55 -0.05448084030911815 1106 56-57 -0.2872833412018352 1106 58-59 -0.14149473521086975 1106 60-61 0.11250160507690765 1106 62-63 -0.15776715568210165 1106 64-65 -0.053679989050671395 1106 66-67 -0.4731362063483644 1106 68-69 -0.19752171402246432 1106 70-71 -0.45648979279202706 1106 72-73 -0.1342498298697734 1106 74-75 -0.08279197723410903 1106 76 -0.04079047445303985 1107 1 -0.7197090623833375 1107 2 -0.23527589970609952 1107 3 -0.2890945650092611 1107 4 -1.5177798998191463 1107 5 -0.7589185060623755 1107 6 -0.5336568683737326 1107 7 -0.06808846175812278 1107 8 -0.19334311795753223 1107 9 0.062287567273010325 1107 10-11 -0.4407903969898328 1107 12-13 0.15719384566904893 1107 14-15 0.1182781695011883 1107 16-17 -0.26455625814455175 1107 18-19 -0.15196341690985093 1107 20-21 0.23621288938073803 1107 22-23 0.06382474671009675 1107 24-25 -0.028675495419108188 1107 26-27 -0.10103512589706298 1107 28-29 0.17904906919964958 1107 30-31 0.0029923804478926286 1107 32-33 0.01988997016258054 1107 34-35 -0.07588172268266646 1107 36-37 -0.3077638598915513 1107 38-39 -0.31430875801518 1107 40-41 -0.2036795323686178 1107 42-43 -0.11952620724886032 1107 44-45 -0.005389986113272016 1107 46-47 0.028817235450716794 1107 48-49 -0.01563913268736883 1107 50-51 0.17667172047904245 1107 52-53 -0.22187468845098834 1107 54-55 0.05963853006677766 1107 56-57 0.07507328027887894 1107 58-59 -0.09872945391587251 1107 60-61 -0.13139084241303323 1107 62-63 0.08077497649923515 1107 64-65 0.008661735960508565 1107 66-67 -0.08888177464867653 1107 68-69 0.013797120080518255 1107 70-71 0.008925570433554242 1107 72-73 0.12196944616180616 1107 74-75 0.17253351062526434 1107 76 0.05851932245842306 1108 1 0.012042762434180787 1108 2 0.03047367918216537 1108 3 0.08201716997108832 1108 4 0.029047725110668665 1108 5 0.21929598018130747 1108 6 0.4378479042259329 1108 7 0.21082787737719144 1108 8 -0.09688045653136612 1108 9 -0.19192355007679396 1108 10-11 0.18924890677210016 1108 12-13 -0.35298302125964653 1108 14-15 -0.08779987654036603 1108 16-17 -0.07460679155274619 1108 18-19 0.030714853724624902 1108 20-21 -0.21823685795221337 1108 22-23 -0.02987261432302546 1108 24-25 -0.05457386712040346 1108 26-27 -0.23051294734568728 1108 28-29 -0.212430436695918 1108 30-31 -0.1789840372949527 1108 32-33 -0.1298573627964288 1108 34-35 0.19298408304445047 1108 36-37 -0.23830849773545992 1108 38-39 0.07197703648229492 1108 40-41 0.10756415095535488 1108 42-43 -0.03930442173510329 1108 44-45 -0.30735517420985303 1108 46-47 -0.06135682406640797 1108 48-49 0.034234533792137256 1108 50-51 -0.15267976744909362 1108 52-53 -0.5241206177042059 1108 54-55 -0.22945467599723202 1108 56-57 3.540214355339799E-4 1108 58-59 -0.11968678126006438 1108 60-61 -0.21347955661853746 1108 62-63 -0.3527174434895315 1108 64-65 -0.005838544780651489 1108 66-67 -0.054729612941770256 1108 68-69 0.288179489535878 1108 70-71 0.09424280794057438 1108 72-73 -0.1175308345793411 1108 74-75 -0.01152039167681096 1108 76 -0.18210392290934863 1109 1 -0.6166183254319293 1109 2 -1.140141061725462 1109 3 -0.6312593025104078 1109 4 -1.27536167302258 1109 5 -1.192644798705075 1109 6 -0.8119911688349006 1109 7 -0.707018920176715 1109 8 -1.3577752103131502 1109 9 -0.685566935979594 1109 10-11 -0.33398894324400175 1109 12-13 -0.992868763132833 1109 14-15 -0.8919759306118138 1109 16-17 -0.896492855279817 1109 18-19 -0.6352651913349163 1109 20-21 -0.9495291012737468 1109 22-23 -0.9514609632126323 1109 24-25 -1.18070035569459 1109 26-27 -1.1634225064058796 1109 28-29 -0.9975688338636033 1109 30-31 -0.8088681698987514 1109 32-33 -0.8528538224037661 1109 34-35 -1.1249914560607976 1109 36-37 -0.9985756364544756 1109 38-39 -1.14449383574172 1109 40-41 -0.9670335316967211 1109 42-43 -0.9473749077344564 1109 44-45 -0.8893957277985223 1109 46-47 -1.060568282064473 1109 48-49 -1.0191207928895452 1109 50-51 -0.9397653805807309 1109 52-53 -0.8064347470894759 1109 54-55 -1.0540040805675588 1109 56-57 -1.05491633582227 1109 58-59 -0.7728811810025817 1109 60-61 -1.0596655881602217 1109 62-63 -1.1274680067338139 1109 64-65 -0.9627825422058223 1109 66-67 -0.5556227574541595 1109 68-69 -1.1259579420701726 1109 70-71 -0.9797513986896149 1109 72-73 -0.7176756688245973 1109 74-75 -0.7872365165561135 1109 76 -1.4536359473702447 1110 1 0.5120427624341808 1110 2 0.7775890637975529 1110 3 0.7974017853556994 1110 4 0.21911182767476944 1110 5 0.5135267494120797 1110 6 0.8955402119182381 1110 7 0.2710842876336059 1110 8 0.8306836460327389 1110 9 1.241089270436028 1110 10-11 0.6751463426695281 1110 12-13 0.687561850535225 1110 14-15 0.9380014055109172 1110 16-17 0.7271560289600743 1110 18-19 0.6539520332118016 1110 20-21 0.6593272446118874 1110 22-23 0.9098709754205601 1110 24-25 1.0103299790334432 1110 26-27 0.6464101295773901 1110 28-29 0.727954178688698 1110 30-31 0.5054710909101772 1110 32-33 1.1009118679727976 1110 34-35 0.7216699804803426 1110 36-37 0.7892556048286394 1110 38-39 0.971336010841263 1110 40-41 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2116 18-19 0.04585604165062307 2116 20-21 -0.06767697479739354 2116 22-23 -0.15268176719547455 2116 24-25 0.16657997903344324 2116 26-27 -0.015615186878306986 2116 28-29 -0.3245774668809247 2116 30-31 -0.1617493732248505 2116 32-33 -0.18010079025504666 2116 34-35 -0.0021960532749289996 2116 36-37 -0.09312836141607761 2116 38-39 0.08821364797206854 2116 40-41 0.1277442872747372 2116 42-43 4.959677488400871E-4 2116 44-45 -0.23788097752046156 2116 46-47 -0.43214552903233283 2116 48-49 -0.5485551448836148 2116 50-51 -0.0973973915970916 2116 52-53 -0.7416473947246587 2116 54-55 0.21467872225009188 2116 56-57 -0.27055639725969627 2116 58-59 -0.30491073452198947 2116 60-61 0.0965496546764939 2116 62-63 0.19509170938291476 2116 64-65 -0.33054643183031374 2116 66-67 -0.5228649586087641 2116 68-69 -0.33910872857511976 2116 70-71 -0.22698406645086067 2116 72-73 -0.19825137985685615 2116 74-75 -0.5408777431860656 2116 76 0.34550075089785537 2117 1 0.40388673406538445 2117 2 0.12218126237911164 2117 3 0.5169053314549963 2117 4 0.6323210475329226 2117 5 0.28143455083051805 2117 6 1.1586607792941237 2117 7 0.49874386210169064 2117 8 0.9290879013518847 2117 9 1.0949013271736163 2117 10-11 0.7414583994071222 2117 12-13 0.867216105854375 2117 14-15 0.6465563700499288 2117 16-17 0.39980673817992596 2117 18-19 0.7981452956231507 2117 20-21 0.711454904186354 2117 22-23 0.35331069173261653 2117 24-25 0.5318282059837998 2117 26-27 0.5261973636199428 2117 28-29 0.7768903489014605 2117 30-31 0.8985118710520226 2117 32-33 0.5792806622990412 2117 34-35 0.5914838102675901 2117 36-37 0.6873052502187136 2117 38-39 0.33906650729516485 2117 40-41 0.7255537854343501 2117 42-43 0.5765575531694296 2117 44-45 0.4672765770176497 2117 46-47 0.5466900935549788 2117 48-49 0.852715166634475 2117 50-51 0.7139187050004452 2117 52-53 0.7086398842052262 2117 54-55 0.4652398139100242 2117 56-57 0.2590692423847969 2117 58-59 0.424216655728479 2117 60-61 0.575991256256529 2117 62-63 0.24583683910730514 2117 64-65 0.2564227754593915 2117 66-67 0.29839368220279994 2117 68-69 0.30794217366025833 2117 70-71 0.4201566104501211 2117 72-73 -0.10899291859462323 2117 74-75 0.12031266887965586 2117 76 -0.2984978127074953 2118 1 1.0540427624341824 2118 2 1.1070890637975488 2118 3 1.2714017853557031 2118 4 1.1902784943414382 2118 5 0.6645267494120759 2118 6 1.1475402119182405 2118 7 0.9817509543002743 2118 8 0.6083503126994003 2118 9 1.175922603769358 2118 10-11 0.3944796760028666 2118 12-13 0.8584785172018954 2118 14-15 0.9065847388442521 2118 16-17 1.2592393622934068 2118 18-19 1.1408686998784745 2118 20-21 1.1039939112785504 2118 22-23 1.1242043087538889 2118 24-25 1.2665799790334447 2118 26-27 1.0764101295773827 2118 28-29 1.3479541786886955 2118 30-31 1.1165544242435033 2118 32-33 1.0029118679727986 2118 34-35 1.2257533138136836 2118 36-37 1.1929222714953056 2118 38-39 0.9106693441746003 2118 40-41 1.1277949201861261 2118 42-43 0.9569263474956742 2118 44-45 0.7631063642516906 2118 46-47 0.7843354836259024 2118 48-49 1.0698499184075203 2118 50-51 0.9026279248585993 2118 52-53 0.7372639976804081 2118 54-55 0.8990837855412295 2118 56-57 1.1844309445124566 2118 58-59 1.3156209110476311 2118 60-61 1.0748281356891525 2118 62-63 1.1902056334335427 2118 64-65 1.2542383782962716 2118 66-67 1.188578079365925 2118 68-69 1.258410258766645 2118 70-71 0.653319731017497 2118 72-73 0.9115460884975732 2118 74-75 1.2772488390924153 2118 76 0.946665307859881 2119 1 0.08171489358172224 2119 2 0.5003349654368954 2119 3 -0.04768018185741241 2119 4 0.4474588222102902 2119 5 0.47705133957601475 2119 6 0.36439267093463457 2119 7 0.07053783954617643 2119 8 0.9789076897485813 2119 9 0.6627422759005057 2119 10-11 0.760119020265158 2119 12-13 0.03178999261172777 2119 14-15 0.3394699847458966 2119 16-17 0.3530098540966762 2119 18-19 0.2932621425014261 2119 20-21 0.19238735390150907 2119 22-23 0.43213873498340405 2119 24-25 0.4247767003449212 2119 26-27 0.4259183262986994 2119 28-29 0.2713968016395114 2119 30-31 0.3005872111287573 2119 32-33 0.47304301551378103 2119 34-35 0.7827697072563069 2119 36-37 0.40469276329858417 2119 38-39 0.16286606548607807 2119 40-41 0.5722867234648135 2119 42-43 0.5762378229055045 2119 44-45 0.7688112822844815 2119 46-47 0.735679745920983 2119 48-49 0.6430302462763748 2119 50-51 0.5982344822356467 2119 52-53 0.46913285013942385 2119 54-55 0.6767559166887693 2119 56-57 0.8181030756599981 2119 58-59 0.4959815667853391 2119 60-61 0.26489370945964197 2119 62-63 0.6143695678597751 2119 64-65 0.5946973946897103 2119 66-67 0.41503709575935943 2119 68-69 0.3127381276191059 2119 70-71 0.5099426818371668 2119 72-73 0.716201826202493 2119 74-75 0.5110521177809417 2119 76 0.6232554717943088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 93.0 14 481.0 15 994.0 16 1172.0 17 1165.0 18 983.0 19 760.0 20 568.0 21 410.0 22 303.0 23 218.0 24 213.0 25 237.0 26 261.0 27 292.0 28 389.0 29 531.0 30 703.0 31 848.0 32 1244.0 33 1855.0 34 2872.0 35 5134.0 36 12103.0 37 51820.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.429547126195224 24.185959627800543 13.652761724633114 25.731731521371117 2 18.942710704820616 23.850886717336227 32.568619896559376 24.637782681283785 3 18.4768776341751 23.849719215906042 29.283270872006817 28.390132277912038 4 14.505037768671267 19.011593289201777 34.21829941741679 32.265069524710164 5 14.255192462610767 33.89490152125436 34.03149918858651 17.818406827548365 6 28.024704330262807 35.74072128238357 18.753575473129956 17.48099891422367 7 26.754462774216897 28.155464490444 22.112477087784434 22.97759564755467 8 23.188913406418923 32.3386221148121 21.720196607240844 22.752267871528144 9 23.530991325464374 17.964344506322018 22.049432010554213 36.455232157659395 10-11 25.407749874493597 24.550803824734686 25.872415443708917 24.1690308570628 12-13 24.65237644916115 22.414859958203447 25.338867290112432 27.593896302522968 14-15 22.992189415432033 23.624391439879513 23.602792663421013 29.78062648126744 16-17 23.710202794998423 25.76150280784094 24.929074288116002 25.599220109044634 18-19 22.923890581765964 25.573535077580473 25.85665417440136 25.645920166252207 20-21 22.70907031861114 25.64942267054277 25.749244042823953 25.892262968022134 22-23 24.106569530547674 25.335364785821863 25.07968197261042 25.478383711020047 24-25 23.47495125681529 25.743406535673007 25.212777135651994 25.568865071859715 26-27 22.292856058748672 26.16429080125623 26.1024132254562 25.440439914538892 28-29 22.912799318179164 25.518078759646478 26.877634175101868 24.69148774707249 30-31 23.13812709420569 25.069174459738715 26.363349795103495 25.4293486509521 32-33 22.516432582629914 25.907440486614597 26.149697033378867 25.42642989737662 34-35 23.119447071322664 25.561276312563486 26.21741211632984 25.10186449978401 36-37 23.497133783988886 25.152650811997248 25.836806650088146 25.513408753925727 38-39 22.51701633334501 25.28866472861429 26.992049315260413 25.20226962278029 40-41 22.817064200903648 24.801816632225375 27.39016730295495 24.990951863916035 42-43 22.34189111881662 24.935495545982043 26.617865106884757 26.10474822831658 44-45 21.884230558182434 25.08668698119155 26.85837040150374 26.17071205912227 46-47 22.12298460065614 24.911561766663166 27.348137251468135 25.617316381212568 48-49 22.13115711066746 25.128133281963272 27.178849544090696 25.56186006327858 50-51 22.05176701341459 25.516327507501195 26.482434940982802 25.94947053810141 52-53 21.767480415163508 25.237294665685965 26.806416587860323 26.188808331290204 54-55 21.38745869963691 25.279908467887875 26.983876805249086 26.348756027226134 56-57 21.678166555754032 25.248385929272764 26.872380418666015 26.201067096307195 58-59 21.632050249261557 25.313182258648265 26.558322533945105 26.49644495814507 60-61 21.42189999182749 24.965850583166965 27.02123685101514 26.5910125739904 62-63 21.141115897866975 25.222700897808597 26.938344249471708 26.69783895485272 64-65 21.751135395140857 25.668686444140892 25.974571818850478 26.60560634186777 66-67 21.574842679182282 25.415338633789826 25.63132639837484 27.378492288653057 68-69 21.184313450783975 25.382648593744527 26.13101701049584 27.30202094497566 70-71 22.178440918590127 24.82750166368954 26.066220681120335 26.927836736600003 72-73 22.217552216501467 25.032398164687752 25.31901976579921 27.43102985301157 74-75 21.820017979522024 25.29508598648033 25.80878661576361 27.07610941823404 76 21.80425671021447 24.647122692725297 25.582291338306888 27.96632925875334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 1.5 14 1.5 15 2.5 16 4.5 17 5.0 18 6.0 19 12.0 20 19.5 21 22.0 22 28.5 23 52.5 24 81.5 25 93.0 26 181.0 27 298.5 28 400.5 29 473.0 30 565.0 31 743.5 32 1088.5 33 1347.0 34 1525.0 35 1889.0 36 2241.5 37 2408.0 38 2739.5 39 3526.0 40 4408.5 41 4859.5 42 4883.0 43 4951.0 44 5249.0 45 5415.0 46 5351.0 47 5293.5 48 5046.0 49 4513.5 50 4171.0 51 3841.0 52 3150.0 53 2516.0 54 2243.0 55 2146.5 56 1825.5 57 1467.0 58 1333.0 59 1209.0 60 1038.5 61 989.0 62 986.0 63 961.0 64 1040.5 65 1163.5 66 1259.5 67 1337.0 68 1325.5 69 1282.0 70 1250.0 71 1250.0 72 1163.5 73 990.5 74 754.0 75 604.0 76 505.0 77 310.0 78 162.5 79 111.0 80 78.5 81 29.5 82 11.0 83 9.0 84 8.0 85 5.5 86 2.0 87 0.0 88 1.0 89 1.5 90 1.5 91 1.0 92 0.0 93 0.5 94 0.5 95 1.5 96 3.0 97 1.5 98 0.0 99 7.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 85653.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.77745087737732 #Duplication Level Percentage of deduplicated Percentage of total 1 70.65042768442534 37.99399904264883 2 13.42755416612392 14.441992691441047 3 5.625027137336634 9.074988616861056 4 2.95471321262646 6.355877785950288 5 2.0646085710564024 5.551469300549893 6 1.3633797924536495 4.399145388953102 7 0.9074725370153272 3.416109184733751 8 0.7164256871173635 3.0822037756996252 9 0.4863010724675438 2.353682883261532 >10 1.804090139377361 13.33053132990088 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0023350028603785038 0.0 5 0.0 0.0 0.0 0.0046700057207570075 0.0 6 0.0 0.0 0.0 0.0046700057207570075 0.0 7 0.0 0.0 0.0 0.0046700057207570075 0.0 8 0.0 0.0 0.0 0.0046700057207570075 0.0 9 0.0 0.0 0.0 0.0046700057207570075 0.0 10 0.0 0.0 0.0 0.0046700057207570075 0.0 11 0.0 0.0 0.0 0.0046700057207570075 0.0 12 0.0 0.0 0.0 0.0046700057207570075 0.0 13 0.0 0.0 0.0 0.0046700057207570075 0.0 14 0.0 0.0 0.0 0.0046700057207570075 0.0 15 0.0 0.0 0.0 0.0046700057207570075 0.0 16 0.0 0.0 0.0 0.00583750715094626 0.0 17 0.0 0.0 0.0 0.00583750715094626 0.0 18 0.0 0.0 0.0 0.00583750715094626 0.0 19 0.0 0.0 0.0 0.010507512871703269 0.0 20 0.0 0.0 0.0 0.010507512871703269 0.0 21 0.0 0.0 0.0 0.012842515732081772 0.0 22 0.0 0.0 0.0 0.015177518592460276 0.0 23 0.0 0.0 0.0 0.01751252145283878 0.0 24 0.0 0.0 0.0 0.022182527173595788 0.0 25 0.0 0.0 0.0 0.022182527173595788 0.0 26 0.0 0.0 0.0 0.022182527173595788 0.0 27 0.0 0.0 0.0 0.033857541475488306 0.0 28 0.0 0.0 0.0 0.04319755291700232 0.0 29 0.0 0.0 0.0 0.05954257293965185 0.0 30 0.0 0.0 0.0 0.08873010869438315 0.0 31 0.0 0.0 0.0 0.10274012585665418 0.0 32 0.0 0.0 0.0 0.12609015446043922 0.0 33 0.0 0.0 0.0 0.15527769021517052 0.0 34 0.0 0.0 0.0 0.2066477531434976 0.0 35 0.0 0.0 0.0 0.2603528189322032 0.0 36 0.0 0.0 0.0 0.3747679590907499 0.0 37 0.0 0.0 0.0 0.54639066932857 0.0 38 0.0 0.0 0.0 0.7355259010192288 0.0 39 0.0 0.0 0.0 0.9725286913476469 0.0 40 0.0 0.0 0.0 1.23288151027985 0.0 41 0.0 0.0 0.0 1.5481068964309481 0.0 42 0.0 0.0 0.0 1.918204849800941 0.0 43 0.0 0.0 0.0 2.2953078117520693 0.0 44 0.0 0.0 0.0 2.6525632493899804 0.0 45 0.0 0.0 0.0 3.084538778560004 0.0 46 0.0 0.0 0.0 3.460474239080943 0.0 47 0.0 0.0 0.0 3.87026724107737 0.0 48 0.0 0.0 0.0 4.243867698737931 0.0 49 0.0 0.0 0.0 4.615133153538113 0.0 50 0.0 0.0 0.0 5.0225911526741625 0.0 51 0.0 0.0 0.0 5.467409197576267 0.0 52 0.0 0.0 0.0 5.956592296825564 0.0 53 0.0 0.0 0.0 6.474962931829592 0.0 54 0.0 0.0 0.0 6.938460999614724 0.0 55 0.0 0.0 0.0 7.35058900447153 0.0 56 0.0 0.0 0.0 7.754544499317012 0.0 57 0.0 0.0 0.0 8.184185025626656 0.0 58 0.0 0.0 0.0 8.583470514751381 0.0 59 0.0 0.0 0.0 8.976918496725158 0.0 60 0.0 0.0 0.0 9.390214003012154 0.0 61 0.0 0.0 0.0 9.80117450643877 0.0 62 0.0 0.0 0.0 10.292692608548446 0.0 63 0.0 0.0 0.0 10.7538556734732 0.0 64 0.0 0.0 0.0 11.283901322779121 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTGGA 20 0.0069302004 52.5 19 TTAGATT 20 0.0069302004 52.5 38 CACATGG 20 0.0069302004 52.5 34 GATTTAC 20 0.0069302004 52.5 41 AAAGTGT 20 0.0069302004 52.5 18 ATTCCGG 20 0.0069302004 52.5 1 CATAGTC 20 0.0069302004 52.5 51 TGGATTC 20 0.0069302004 52.5 24 AGTTAGA 20 0.0069302004 52.5 36 AAGGGGG 20 0.0069302004 52.5 66 GCAGAGG 20 0.0069302004 52.5 8 TGGGTTC 35 0.0012492745 40.0 38 GAGAATT 35 0.0012492745 40.0 16 AGGTAAT 40 0.0024069962 35.0 45 CATTCTT 40 0.0024069962 35.0 14 TAACATA 40 0.0024069962 35.0 5 GTGTATT 40 0.0024069962 35.0 1 GCGAAGA 45 0.004286448 31.111113 26 GGTCATT 45 0.004286448 31.111113 51 AGTGGAA 45 0.004286448 31.111113 20 >>END_MODULE