Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780338_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 85653 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG | 1985 | 2.317490338925665 | Illumina PCR Primer Index 10 (96% over 26bp) |
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT | 1815 | 2.1190150957934923 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG | 459 | 0.5358831564568667 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC | 230 | 0.26852532894352793 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC | 224 | 0.2615203203623925 | No Hit |
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT | 179 | 0.20898275600387609 | Illumina PCR Primer Index 10 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTAATAC | 15 | 0.0022178618 | 70.0 | 3 |
CAATTCC | 15 | 0.0022178618 | 70.0 | 70 |
ACGATCT | 15 | 0.0022178618 | 70.0 | 61 |
CTTAATA | 25 | 2.378522E-4 | 55.999996 | 2 |
CTATGGG | 20 | 0.0069302004 | 52.5 | 6 |
CAGAACA | 20 | 0.0069302004 | 52.5 | 51 |
TCGATTA | 20 | 0.0069302004 | 52.5 | 44 |
ACAGGCT | 20 | 0.0069302004 | 52.5 | 55 |
GTTGTCA | 20 | 0.0069302004 | 52.5 | 22 |
ATCGATT | 20 | 0.0069302004 | 52.5 | 43 |
TCTGCAA | 30 | 5.848813E-4 | 46.666664 | 32 |
TGCAAAA | 30 | 5.848813E-4 | 46.666664 | 59 |
GTTGATC | 35 | 0.0012492745 | 40.0 | 29 |
TTGATCG | 35 | 0.0012492745 | 40.0 | 30 |
GCAAGAG | 35 | 0.0012492745 | 40.0 | 14 |
TATGGGT | 35 | 0.0012492745 | 40.0 | 7 |
GCTGAGC | 35 | 0.0012492745 | 40.0 | 9 |
TGTTGTC | 35 | 0.0012492745 | 40.0 | 21 |
CAAGGTT | 35 | 0.0012492745 | 40.0 | 36 |
GTGAGGT | 35 | 0.0012492745 | 40.0 | 24 |