FastQCFastQC Report
Wed 25 May 2016
SRR1780338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85653
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG19852.317490338925665Illumina PCR Primer Index 10 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT18152.1190150957934923No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG4590.5358831564568667No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2300.26852532894352793No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2240.2615203203623925No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT1790.20898275600387609Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC150.002217861870.03
CAATTCC150.002217861870.070
ACGATCT150.002217861870.061
CTTAATA252.378522E-455.9999962
CTATGGG200.006930200452.56
CAGAACA200.006930200452.551
TCGATTA200.006930200452.544
ACAGGCT200.006930200452.555
GTTGTCA200.006930200452.522
ATCGATT200.006930200452.543
TCTGCAA305.848813E-446.66666432
TGCAAAA305.848813E-446.66666459
GTTGATC350.001249274540.029
TTGATCG350.001249274540.030
GCAAGAG350.001249274540.014
TATGGGT350.001249274540.07
GCTGAGC350.001249274540.09
TGTTGTC350.001249274540.021
CAAGGTT350.001249274540.036
GTGAGGT350.001249274540.024