##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780338_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85653 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.37453445880471 34.0 34.0 34.0 33.0 34.0 2 33.530734475149735 34.0 34.0 34.0 34.0 34.0 3 33.60838499527162 34.0 34.0 34.0 34.0 34.0 4 33.601555111905014 34.0 34.0 34.0 34.0 34.0 5 33.530197424491845 34.0 34.0 34.0 34.0 34.0 6 37.09644729314793 38.0 37.0 38.0 36.0 38.0 7 37.160963422180195 38.0 37.0 38.0 36.0 38.0 8 36.91234399262139 38.0 37.0 38.0 35.0 38.0 9 37.01444199269144 38.0 37.0 38.0 35.0 38.0 10-11 36.93896886273686 38.0 37.0 38.0 35.0 38.0 12-13 36.93575823380384 38.0 37.0 38.0 35.0 38.0 14-15 36.8556267731428 38.0 37.0 38.0 34.5 38.0 16-17 36.8698469405625 38.0 37.0 38.0 34.5 38.0 18-19 36.85612296125063 38.0 37.0 38.0 34.5 38.0 20-21 36.879122739425355 38.0 37.0 38.0 35.0 38.0 22-23 36.81868702789161 38.0 37.0 38.0 34.5 38.0 24-25 36.83562163613651 38.0 37.0 38.0 34.5 38.0 26-27 36.70187267229402 38.0 37.0 38.0 34.0 38.0 28-29 36.638185469277204 38.0 37.0 38.0 34.0 38.0 30-31 36.58154413739157 38.0 37.0 38.0 34.0 38.0 32-33 36.53809557166707 38.0 37.0 38.0 34.0 38.0 34-35 36.539134647939946 38.0 37.0 38.0 34.0 38.0 36-37 36.56551434275507 38.0 37.0 38.0 34.0 38.0 38-39 36.31049700535883 38.0 37.0 38.0 34.0 38.0 40-41 36.45384283095747 38.0 37.0 38.0 34.0 38.0 42-43 36.47582688288793 38.0 37.0 38.0 34.0 38.0 44-45 36.53564381866368 38.0 37.0 38.0 34.0 38.0 46-47 36.40495370856829 38.0 37.0 38.0 34.0 38.0 48-49 36.46658027156083 38.0 37.0 38.0 34.0 38.0 50-51 36.23664086488506 38.0 37.0 38.0 34.0 38.0 52-53 36.28620130059659 38.0 37.0 38.0 34.0 38.0 54-55 36.20487315096961 38.0 37.0 38.0 34.0 38.0 56-57 36.28926015434369 38.0 37.0 38.0 34.0 38.0 58-59 36.1634852252694 38.0 37.0 38.0 34.0 38.0 60-61 36.185895415221886 38.0 37.0 38.0 34.0 38.0 62-63 36.09602115512591 38.0 37.0 38.0 33.5 38.0 64-65 35.99022217552216 38.0 37.0 38.0 32.5 38.0 66-67 35.53877272249659 38.0 36.5 38.0 30.5 38.0 68-69 35.53843414708183 38.0 36.0 38.0 31.0 38.0 70-71 35.193968687611644 38.0 36.0 38.0 29.0 38.0 72-73 34.85517144758502 38.0 36.0 38.0 26.0 38.0 74-75 34.688784981261605 38.0 36.0 38.0 25.0 38.0 76 33.72720161582198 37.0 34.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10192092825241161 1101 2 -0.12717744925665642 1101 3 -0.5272209887468549 1101 4 -0.0567386290902121 1101 5 -0.31145038676475423 1101 6 -0.0030255964164069837 1101 7 0.0017013719773331104 1101 8 -0.3149673533038566 1101 9 -0.15201990111418695 1101 10-11 -0.25108847412884217 1101 12-13 -0.06397546816332067 1101 14-15 0.3098742250200175 1101 16-17 -0.009602073217536145 1101 18-19 0.1209216722266504 1101 20-21 -0.01633794648228104 1101 22-23 0.1490211511232431 1101 24-25 -0.23187664643101868 1101 26-27 -0.2297777142410382 1101 28-29 -0.6546985589675245 1101 30-31 -0.06845343081417354 1101 32-33 -0.14817826100080111 1101 34-35 0.015369324241476079 1101 36-37 -0.16230699202774446 1101 38-39 -0.6135682489128058 1101 40-41 -0.21432601397795992 1101 42-43 0.02447009759735863 1101 44-45 -0.17746986819336286 1101 46-47 0.027706735401231697 1101 48-49 -0.17970963909544935 1101 50-51 -0.1304554765428776 1101 52-53 0.23476264743483455 1101 54-55 0.10109082631013422 1101 56-57 -0.3613089341699194 1101 58-59 -0.1447154521437568 1101 60-61 -0.3271525167491163 1101 62-63 -0.20034914616326915 1101 64-65 0.061433196401750934 1101 66-67 -0.07801834330146562 1101 68-69 -0.6614991722264705 1101 70-71 -0.9040594119933445 1101 72-73 -0.7886592509813184 1101 74-75 -0.9777913195095493 1101 76 -1.596407424386058 1102 1 0.2135690612101797 1102 2 0.0711892525347011 1102 3 -0.12895966103346979 1102 4 0.09868813592559889 1102 5 0.009887864025550641 1102 6 0.154982833509834 1102 7 0.27888788409535437 1102 8 0.2810811187720148 1102 9 0.17437630541900973 1102 10-11 0.22504429720941488 1102 12-13 0.47106141276396585 1102 14-15 0.4877456686448838 1102 16-17 0.3051766412187149 1102 18-19 0.4618858450401362 1102 20-21 0.3271552042026471 1102 22-23 0.31935834817277 1102 24-25 0.08145317443305089 1102 26-27 0.36732449861459315 1102 28-29 0.4331570785456478 1102 30-31 0.49000283894346097 1102 32-33 0.5339081457431334 1102 34-35 0.23625973520037746 1102 36-37 0.3762072334727904 1102 38-39 0.2992610450808755 1102 40-41 0.3327498553581876 1102 42-43 0.6142224685141073 1102 44-45 0.49342054276554137 1102 46-47 0.4013632159070184 1102 48-49 0.5989784536337339 1102 50-51 0.5918838279882053 1102 52-53 0.55372998147066 1102 54-55 0.27717091060939936 1102 56-57 0.5013622987067947 1102 58-59 0.6111064656644629 1102 60-61 0.7923943747155775 1102 62-63 0.6089764597376828 1102 64-65 0.8216808254850037 1102 66-67 0.9125770202390981 1102 68-69 1.0141067287218988 1102 70-71 0.461904760820147 1102 72-73 0.44969691340224216 1102 74-75 0.1916238543576796 1102 76 0.3043301941597818 1103 1 -0.2324285159028534 1103 2 0.07428775220854789 1103 3 -0.24789079513094237 1103 4 -0.058962595599886924 1103 5 0.030412512240999945 1103 6 -0.08777180259118467 1103 7 -0.12522170494574425 1103 8 0.05190862785784134 1103 9 -0.007200434866149408 1103 10-11 0.19672680582929303 1103 12-13 0.26955409750910064 1103 14-15 0.13363142020578067 1103 16-17 0.2123497844486124 1103 18-19 0.02250461309530749 1103 20-21 -6.916879788789743E-4 1103 22-23 0.252030046989276 1103 24-25 -0.03366890179469806 1103 26-27 0.23259800527753782 1103 28-29 0.159207772795952 1103 30-31 -0.06320748628252204 1103 32-33 0.06778196924513935 1103 34-35 -0.07986877099661882 1103 36-37 0.03160980546048364 1103 38-39 -0.3546854650300233 1103 40-41 -0.011055474993135306 1103 42-43 -0.04057438173283856 1103 44-45 0.12627162840528428 1103 46-47 -0.1961812813439323 1103 48-49 -0.12573266368990232 1103 50-51 -0.354160343104887 1103 52-53 -0.17141213016851253 1103 54-55 -0.06811377860874757 1103 56-57 -0.23528852600664862 1103 58-59 -0.13230048615735512 1103 60-61 -0.2768908736303288 1103 62-63 -0.045482584153845096 1103 64-65 0.18566134920133948 1103 66-67 -0.2889419435107783 1103 68-69 -0.15582151654881216 1103 70-71 -0.4298574235056378 1103 72-73 -0.6221626523418635 1103 74-75 -0.5029875518486406 1103 76 -0.4537072674001834 1104 1 0.07766779985117012 1104 2 0.09762580029907042 1104 3 0.0944342537004701 1104 4 0.3019183904578995 1104 5 0.24648309889150255 1104 6 0.40569489101694955 1104 7 0.22131294851960348 1104 8 0.01351703359637213 1104 9 0.1179090148543338 1104 10-11 -0.06798997197093826 1104 12-13 0.2133474564368214 1104 14-15 0.13179603258539174 1104 16-17 0.09527226036967562 1104 18-19 0.022184683143130712 1104 20-21 -0.0643577484624771 1104 22-23 0.21995285967871325 1104 24-25 0.3037224904057467 1104 26-27 0.3356322882976741 1104 28-29 0.17333520594742424 1104 30-31 0.11280450267148012 1104 32-33 -0.14010514599699775 1104 34-35 0.34045183249229893 1104 36-37 0.3037186489978936 1104 38-39 -0.055298383464716494 1104 40-41 -0.09689773268829072 1104 42-43 0.018326406305156695 1104 44-45 0.3624641252059817 1104 46-47 -0.14724883707909697 1104 48-49 0.20175773841152989 1104 50-51 -0.013738035568010787 1104 52-53 0.20288907546225232 1104 54-55 -0.06796223284193559 1104 56-57 -0.5998402873052342 1104 58-59 0.27582910231168967 1104 60-61 -0.12933581200172029 1104 62-63 0.25168182616474155 1104 64-65 -0.24331420141494675 1104 66-67 -0.2907436542941042 1104 68-69 -0.2673255240924277 1104 70-71 0.18309468803204965 1104 72-73 0.5109282077645574 1104 74-75 0.44595836315102133 1104 76 0.7259586583760651 1105 1 -0.05684670423082139 1105 2 0.08807221336142845 1105 3 0.05132152137460366 1105 4 0.23104814553867214 1105 5 0.242800625380994 1105 6 0.022682905607886994 1105 7 0.2537256634753078 1105 8 0.07167232280950486 1105 9 0.2843498694661122 1105 10-11 0.3378116608239239 1105 12-13 0.22404747380699064 1105 14-15 0.16540728844781682 1105 16-17 0.09296202934657316 1105 18-19 0.4596868544128867 1105 20-21 0.1424339833422792 1105 22-23 0.1194664952527944 1105 24-25 0.1707886707079851 1105 26-27 0.44586331139998947 1105 28-29 0.2283986070243742 1105 30-31 -0.02928339032834515 1105 32-33 0.18353563913414206 1105 34-35 0.11186055231165426 1105 36-37 0.48968356127860346 1105 38-39 0.20245739211283365 1105 40-41 0.2696820831880373 1105 42-43 0.07399776291449456 1105 44-45 0.09445995636804838 1105 46-47 0.0781115937008181 1105 48-49 0.16644420705839735 1105 50-51 -0.15643388410023107 1105 52-53 0.17976939507315848 1105 54-55 -0.1752924395468085 1105 56-57 0.31018229390026164 1105 58-59 0.361863495224668 1105 60-61 0.25921725383117433 1105 62-63 0.16840928838463753 1105 64-65 0.48558974161093005 1105 66-67 -0.0659738088885149 1105 68-69 0.6767599370852722 1105 70-71 0.39742506843850833 1105 72-73 0.5310775911239389 1105 74-75 0.07409801922189274 1105 76 -0.31138718147107625 1106 1 0.18934019158013626 1106 2 0.09809727601806628 1106 3 0.09904798037926099 1106 4 0.07709182616881805 1106 5 0.040616593873650686 1106 6 -0.06396227878165917 1106 7 -0.07079993623825942 1106 8 0.29000386595307504 1106 9 0.0914390209161624 1106 10-11 0.21203292827826914 1106 12-13 0.39540443764514777 1106 14-15 0.13363142020578067 1106 16-17 0.24636338989078865 1106 18-19 0.06502161989802602 1106 20-21 0.27651919637485634 1106 22-23 -0.0013713135549409117 1106 24-25 0.257147424735912 1106 26-27 0.11014902568570051 1106 28-29 0.15580641225173508 1106 30-31 0.2174047586154444 1106 32-33 0.6307071393131665 1106 34-35 0.6854373514523573 1106 36-37 0.3241268122632093 1106 38-39 -0.13019566911165725 1106 40-41 0.17772003521094604 1106 42-43 0.255343985614104 1106 44-45 0.2555233290855554 1106 46-47 0.3038187186560677 1106 48-49 0.6072605335890131 1106 50-51 0.3890369358066792 1106 52-53 0.25035657731448424 1106 54-55 0.6410698948606353 1106 56-57 0.5266162358981106 1106 58-59 0.34048862948889536 1106 60-61 0.36256490868259306 1106 62-63 0.4222044906760871 1106 64-65 0.8472259750516713 1106 66-67 0.9236430905028215 1106 68-69 0.7659471909341846 1106 70-71 0.8150405356780368 1106 72-73 0.5360006129642585 1106 74-75 0.3881689107363968 1106 76 0.386428787021579 1107 1 -0.14734484739895493 1107 2 -0.04632399614042981 1107 3 -0.0534422127782932 1107 4 0.12476052868632337 1107 5 0.0963542173902141 1107 6 0.08319001279190275 1107 7 0.053610411842576866 1107 8 0.22046218066582668 1107 9 0.36215752729682293 1107 10-11 0.1499643052086057 1107 12-13 0.09948607029821233 1107 14-15 0.30338910430132415 1107 16-17 0.07751190769207028 1107 18-19 0.04802723090155325 1107 20-21 0.39643577614545933 1107 22-23 -0.15636907908451292 1107 24-25 0.05266855289520578 1107 26-27 0.16107012405204557 1107 28-29 0.1334120530481968 1107 30-31 -0.20965219555309034 1107 32-33 0.05350618593911349 1107 34-35 -0.16528761006509285 1107 36-37 -0.005892056598206352 1107 38-39 -0.16116791202341574 1107 40-41 0.05249038130559569 1107 42-43 0.2769686938915754 1107 44-45 0.11924546027379535 1107 46-47 0.025825769651646624 1107 48-49 0.1211887326058445 1107 50-51 0.21864615175582713 1107 52-53 0.09835051941686856 1107 54-55 0.474701157584704 1107 56-57 -0.08010455460787824 1107 58-59 0.21714586172485895 1107 60-61 -0.21005538030891557 1107 62-63 -0.1402986127550676 1107 64-65 0.23010248331921446 1107 66-67 0.24914336360476597 1107 68-69 0.5565524841463514 1107 70-71 0.34366658463776645 1107 72-73 0.0067787052230627864 1107 74-75 0.1914113756055542 1107 76 0.08815181199799582 1108 1 0.06346368713220585 1108 2 0.09205331997411292 1108 3 0.24585593433007347 1108 4 0.18172290937133084 1108 5 -0.14606577138013677 1108 6 0.08543594204513028 1108 7 -0.05599093571497349 1108 8 0.5081095697730689 1108 9 0.07105702196273711 1108 10-11 0.0681422951019286 1108 12-13 -0.023590852778710314 1108 14-15 0.013720378866175054 1108 16-17 -0.10960207321753757 1108 18-19 0.03630628761126786 1108 20-21 -0.0028764080207466236 1108 22-23 -0.15674807964599324 1108 24-25 0.11427719972283512 1108 26-27 0.12599151652818819 1108 28-29 0.2683783641093953 1108 30-31 -0.029991892352640548 1108 32-33 -0.0770244148469601 1108 34-35 -0.11347682960467864 1108 36-37 0.12423146951071828 1108 38-39 -0.26549132583588175 1108 40-41 -0.133556783208725 1108 42-43 -0.03322221009494797 1108 44-45 0.07253013180663714 1108 46-47 0.07386058155507413 1108 48-49 -8.634852492974687E-4 1108 50-51 0.3272368311494276 1108 52-53 -0.05562196794978291 1108 54-55 -0.3123707121513988 1108 56-57 -0.00553970340068588 1108 58-59 -0.3908692982976021 1108 60-61 0.14207825248165307 1108 62-63 0.07849700768288415 1108 64-65 0.3652793502479099 1108 66-67 0.23544319516007306 1108 68-69 0.4269623662350739 1108 70-71 0.49786366492973855 1108 72-73 0.5921099797879066 1108 74-75 0.10297791125968558 1108 76 0.015131037152400495 1109 1 -0.004978540096630013 1109 2 0.18775657069828355 1109 3 0.03669919149131573 1109 4 -0.06550914727495183 1109 5 0.22575592157126323 1109 6 -0.31446750179136274 1109 7 0.04632641220906919 1109 8 0.1261977577357314 1109 9 -0.24334593909294 1109 10-11 -0.09871480177609016 1109 12-13 -0.021852841835681147 1109 14-15 0.15157039495886693 1109 16-17 0.044565934506955784 1109 18-19 -0.12820127595487207 1109 20-21 0.1524905054005643 1109 22-23 -0.16890610990024868 1109 24-25 -0.3247556293727598 1109 26-27 0.14098186091183607 1109 28-29 0.019303694009622063 1109 30-31 0.15239465415524478 1109 32-33 0.07402965660460126 1109 34-35 0.149260537630596 1109 36-37 -0.03170673454464179 1109 38-39 -0.3162315897560575 1109 40-41 -0.06751236737351718 1109 42-43 -0.0520023838960455 1109 44-45 -0.21387154183352663 1109 46-47 0.1627405300584499 1109 48-49 0.11456136187011623 1109 50-51 -0.1868603687218311 1109 52-53 0.048843754612178714 1109 54-55 -0.5978792412791734 1109 56-57 -0.13568131910715664 1109 58-59 0.012292916057084824 1109 60-61 -0.008645918744612402 1109 62-63 -0.21156575382339327 1109 64-65 -0.13029515892492327 1109 66-67 0.11041905611919844 1109 68-69 0.5451149249734044 1109 70-71 0.38750801639417176 1109 72-73 -0.5540358200189743 1109 74-75 -0.46541603788740815 1109 76 0.10922501848487798 1110 1 -0.06890810773958833 1110 2 -0.1649979620771731 1110 3 0.13399695997109262 1110 4 0.1323639350123571 1110 5 -0.21048884830321413 1110 6 -0.9007820066728129 1110 7 -0.30855503827907427 1110 8 -0.18644171227821715 1110 9 0.02426215016786415 1110 10-11 -0.23137693566730633 1110 12-13 0.14740273696487805 1110 14-15 -0.5462155185697313 1110 16-17 -0.09453797065342684 1110 18-19 0.1040947491497306 1110 20-21 0.0378287201843861 1110 22-23 -0.1154019257998371 1110 24-25 0.11475796895359736 1110 26-27 0.34169664473331807 1110 28-29 0.06677580000683747 1110 30-31 0.1035016973909535 1110 32-33 0.11336020053765594 1110 34-35 -0.11796400909185678 1110 36-37 -0.07753135100210073 1110 38-39 -0.2954592745538349 1110 40-41 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-0.12122427420290904 2116 1 -0.31642920478600445 2116 2 -0.04295154857506134 2116 3 -0.08651991766603828 2116 4 -0.08229851224503193 2116 5 -0.25146986096144275 2116 6 -0.1755182935926456 2116 7 -0.11889259102169092 2116 8 0.10538318223655097 2116 9 -0.02373363042285348 2116 10-11 -0.12750035338881816 2116 12-13 -0.2897544360309041 2116 14-15 -0.15882100380179764 2116 16-17 -0.786573835632332 2116 18-19 -0.2284210736350829 2116 20-21 -0.21443921230506646 2116 22-23 0.11197149192167899 2116 24-25 0.22496366515613175 2116 26-27 -0.20645736370549628 2116 28-29 0.1277990067578969 2116 30-31 0.12378123325592583 2116 32-33 -0.5752473943990495 2116 34-35 -0.41184586563194614 2116 36-37 -0.35556405142404657 2116 38-39 0.26406076764025954 2116 40-41 -0.2952413012223545 2116 42-43 -0.02066135323030238 2116 44-45 -0.33822936186424357 2116 46-47 -0.03577914580617403 2116 48-49 -0.11710009673907251 2116 50-51 -0.2076219809245785 2116 52-53 -0.2731730877160885 2116 54-55 -0.47334929053504027 2116 56-57 -0.9192203265749797 2116 58-59 -0.5621205154348914 2116 60-61 -0.5570940941590479 2116 62-63 -0.22941438472217612 2116 64-65 -0.34406826416299907 2116 66-67 -0.22336887884188172 2116 68-69 -1.3039529210093264 2116 70-71 -0.6208072211462081 2116 72-73 -0.5123934281965106 2116 74-75 -0.3308098199964036 2116 76 -0.3360374146489562 2117 1 -0.45445775312965964 2117 2 0.03730700246183716 2117 3 -0.26431864286578133 2117 4 -0.3206502493848049 2117 5 0.22204661268968806 2117 6 -0.05122526908416347 2117 7 -0.004654329059221141 2117 8 -0.729970081072544 2117 9 -0.4996740200448997 2117 10-11 -0.24613757396517855 2117 12-13 -0.5104430077159776 2117 14-15 -0.6645665823995088 2117 16-17 -0.34391740327754405 2117 18-19 -0.03318361964459626 2117 20-21 -0.004635818822706028 2117 22-23 0.037346301150520844 2117 24-25 -0.4301445133159092 2117 26-27 -0.2725764049120727 2117 28-29 -1.0944830652413913 2117 30-31 0.056382903064715606 2117 32-33 -0.37316462215738255 2117 34-35 -0.0768292573188063 2117 36-37 -0.054437379370895655 2117 38-39 -0.13584048022758566 2117 40-41 -0.16585356444168298 2117 42-43 0.15873087228366245 2117 44-45 -0.1086755419522305 2117 46-47 -0.14335163884863533 2117 48-49 -0.07894586386359492 2117 50-51 -0.2230632234059442 2117 52-53 -0.3311675216868224 2117 54-55 0.20649180830686475 2117 56-57 0.23142156228399102 2117 58-59 0.09624199466475858 2117 60-61 -0.1921825222046536 2117 62-63 -0.16033277955115466 2117 64-65 -0.9302743867951904 2117 66-67 -0.0280865375185968 2117 68-69 -0.3660545459307798 2117 70-71 -0.37536328542486785 2117 72-73 -0.43812733608770316 2117 74-75 0.46849891507855546 2117 76 -0.21582913742479093 2118 1 -0.17207477440625496 2118 2 -0.5133312954105023 2118 3 -0.3238363733622407 2118 4 -0.5519693983209777 2118 5 -0.3129888483032204 2118 6 -0.6219486733394817 2118 7 -0.2932217049457435 2118 8 -0.22727504561154888 2118 9 0.09459548350120173 2118 10-11 -0.31362693566730826 2118 12-13 0.11948607029820835 2118 14-15 -0.04104885190306362 2118 16-17 -0.10437130398675976 2118 18-19 -0.08915525085027554 2118 20-21 -0.37033794648228735 2118 22-23 -0.1879019257998351 2118 24-25 -0.24949203104640105 2118 26-27 -0.5154700219333535 2118 28-29 -0.2193908666598361 2118 30-31 -0.3819149692757122 2118 32-33 -0.6026397994623451 2118 34-35 -0.7846306757585211 2118 36-37 -0.3696146843354384 2118 38-39 -0.45087594122049524 2118 40-41 -0.38863370628565264 2118 42-43 -0.19891451778725866 2118 44-45 -0.3174698681933634 2118 46-47 -0.16167787998339378 2118 48-49 -0.46555579294160054 2118 50-51 0.15339067730327116 2118 52-53 0.1598395705117568 2118 54-55 -0.3376784044590906 2118 56-57 -0.07030893416991546 2118 58-59 -0.47371545214375743 2118 60-61 0.021462867866269164 2118 62-63 -0.06450299231711654 2118 64-65 -0.491182188213628 2118 66-67 0.103289349006225 2118 68-69 -0.6093453260726207 2118 70-71 -0.04313633507025827 2118 72-73 0.2883407490186798 2118 74-75 0.8457471420289124 2118 76 -0.4831766551552903 2119 1 0.004384241987182236 2119 2 0.01847198327801891 2119 3 0.18324559385087724 2119 4 0.2027191262691872 2119 5 -0.22708720895895596 2119 6 0.06021526108674635 2119 7 -0.018664327896566135 2119 8 -0.04661930790663149 2119 9 0.1629233523536584 2119 10-11 -0.2081187389459913 2119 12-13 -0.3103499952755584 2119 14-15 -0.6654095076407671 2119 16-17 -0.05348605808512019 2119 18-19 -0.34846672626010644 2119 20-21 -0.23109204484293855 2119 22-23 0.40980299223295447 2119 24-25 -0.5013280966201705 2119 26-27 0.07892342068959834 2119 28-29 -0.13247283387294573 2119 30-31 -0.7089641496035881 2119 32-33 -0.7120496355279258 2119 34-35 -0.07069624952901421 2119 36-37 -0.5951884548272375 2119 38-39 -0.11366282646639547 2119 40-41 0.14582531010778865 2119 42-43 -0.30075058336102956 2119 44-45 -0.3709124911441748 2119 46-47 -0.1712844373604412 2119 48-49 -0.1753590716301261 2119 50-51 -0.05447817515574371 2119 52-53 -0.3454391180128269 2119 54-55 0.06950192340976002 2119 56-57 -0.14696467187483364 2119 58-59 0.2565140560529642 2119 60-61 0.07467598262037001 2119 62-63 -0.30551938575973736 2119 64-65 -0.46416579477100584 2119 66-67 -0.37874343787902376 2119 68-69 -0.5596076211545906 2119 70-71 -0.1394314170374713 2119 72-73 0.5830620604940862 2119 74-75 0.44850124038956807 2119 76 -1.9550455076143045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 2.0 21 2.0 22 4.0 23 10.0 24 28.0 25 46.0 26 109.0 27 223.0 28 344.0 29 642.0 30 960.0 31 1634.0 32 2613.0 33 4344.0 34 7022.0 35 9579.0 36 17324.0 37 40764.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.32268572029 20.44645254690437 12.968605886542212 29.262255846263415 2 20.4756400826591 22.1486696321203 33.277293264684246 24.09839702053635 3 19.24509357523963 22.46272751684121 30.025801781607182 28.26637712631198 4 15.45421643141513 22.13115711066746 34.42494717056028 27.989679287357127 5 15.113306013799868 33.113843064457754 36.1832043244253 15.589646597317081 6 28.833782821383956 36.10264672574224 17.928151961986153 17.135418490887652 7 27.98500928163637 27.508668698119155 23.842714207324903 20.663607812919572 8 23.9734743675061 33.797998902548656 18.988243260597994 23.24028346934725 9 23.585863892683268 16.98714580925362 20.816550500274364 38.61043979778876 10-11 26.88930918940376 26.104164477601483 24.945419308138653 22.061107024856106 12-13 26.00667810818068 21.66298903716157 25.167828330589703 27.162504524068044 14-15 23.002113177588644 22.081538299884418 22.246156001541102 32.670192520985836 16-17 24.76971034289517 27.083114426815175 23.057569495522632 25.089605734767023 18-19 25.93779552379952 26.14736203051849 23.930276814589096 23.984565631092895 20-21 24.143345825598637 25.614397627637093 23.542666339766267 26.699590206998003 22-23 23.56718386980024 26.961110527360393 23.800100405122997 25.671605197716367 24-25 26.17071205912227 25.766756564276793 24.77963410505178 23.282897271549157 26-27 25.781934082869252 27.66161138547395 23.9623831039193 22.5940714277375 28-29 23.267719752956697 27.261158394919033 26.060966924684482 23.410154927439784 30-31 24.786639113632912 25.88759296230138 26.523297491039425 22.802470433026283 32-33 23.855556723056985 26.46608992096015 26.020104374627863 23.658248981355 34-35 23.818196677290928 25.90802423732969 26.9932168166906 23.28056226868878 36-37 24.7125027728159 25.349958553699224 27.381411042228525 22.55612763125635 38-39 22.56897014698843 26.733447748473495 27.17476328908503 23.522818815453046 40-41 23.898170525258895 23.746395339334292 26.91499422086792 25.440439914538892 42-43 25.715386501348465 23.570102623375714 26.2874622021412 24.427048673134625 44-45 22.619172708486566 25.501733739623834 27.882852906494808 23.99624064539479 46-47 24.18537587708545 24.27001973077417 27.80463031067213 23.739974081468247 48-49 23.96063185177402 23.373378632388825 26.20923960631852 26.45674990951864 50-51 24.130503309866555 23.393226156702042 26.475429932401667 26.000840601029733 52-53 21.566086418455864 23.663502737790854 31.22249074755117 23.54792009620212 54-55 22.978179398269763 24.599838884802633 27.83732035071743 24.584661366210174 56-57 22.759856630824373 26.067388182550523 26.044621904661835 25.128133281963272 58-59 21.949026887557938 26.196397090586437 27.93072046513257 23.923855556723055 60-61 23.003864429733927 27.325954724294537 26.140357021937348 23.529823824034185 62-63 21.114847115687716 30.13904942033554 24.995038118921695 23.751065345055046 64-65 21.232181009421737 30.956884172183113 24.065123229775956 23.745811588619194 66-67 21.217587241544372 30.06608058094871 24.241415945734534 24.474916231772383 68-69 20.707972867266765 30.26689082694126 24.46148996532521 24.563646340466764 70-71 21.449336275436938 29.260504594118125 24.458571211749735 24.8315879186952 72-73 21.1276896314198 28.408228550079972 24.511692526823346 25.95238929167688 74-75 21.103172101385827 28.260539619161033 24.847349188002752 25.788939091450384 76 21.33492113527839 28.23835709198744 24.901638004506555 25.525083768227617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.0 15 4.0 16 5.5 17 5.0 18 10.5 19 16.5 20 16.5 21 16.0 22 31.0 23 61.0 24 78.5 25 81.0 26 150.0 27 272.0 28 378.5 29 432.0 30 510.5 31 714.0 32 1051.0 33 1263.0 34 1381.0 35 1684.5 36 2101.5 37 2333.0 38 3249.0 39 4768.0 40 5576.0 41 5550.5 42 5320.0 43 5240.0 44 5172.0 45 5150.0 46 5116.0 47 5028.5 48 4807.0 49 4293.5 50 3914.0 51 3500.0 52 2817.5 53 2279.0 54 2009.0 55 1921.0 56 1640.0 57 1327.0 58 1207.0 59 1110.5 60 920.5 61 867.0 62 907.0 63 914.5 64 967.5 65 1091.5 66 1227.5 67 1285.0 68 1341.5 69 1322.0 70 1245.5 71 1245.0 72 1160.5 73 952.5 74 710.0 75 591.0 76 479.5 77 284.5 78 158.0 79 115.0 80 77.0 81 24.5 82 7.5 83 5.0 84 2.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 85653.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.43448565724493 #Duplication Level Percentage of deduplicated Percentage of total 1 64.54414636653306 29.32530092349363 2 15.916332613834927 14.463007717184453 3 6.7864117586596775 9.250113831389443 4 3.6488847774694215 6.631408123474951 5 2.515674786720115 5.714919500776388 6 1.4955288313290163 4.076914994220868 7 1.174324185425018 3.734837075175417 8 0.9584746633775312 3.483824267684728 9 0.6192825573029088 2.5323106020804875 >10 2.310103813341556 14.63930043314303 >50 0.01541782300339192 0.4366455348907803 >100 0.010278548668927948 1.274911561766663 >500 0.0 0.0 >1k 0.005139274334463974 4.436505434719158 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG 1985 2.317490338925665 Illumina PCR Primer Index 10 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 1815 2.1190150957934923 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 459 0.5358831564568667 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 230 0.26852532894352793 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 224 0.2615203203623925 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 179 0.20898275600387609 Illumina PCR Primer Index 10 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0023350028603785038 0.0 5 0.0 0.0 0.0 0.003502504290567756 0.0 6 0.0 0.0 0.0 0.003502504290567756 0.0 7 0.0 0.0 0.0 0.003502504290567756 0.0 8 0.0 0.0 0.0 0.003502504290567756 0.0 9 0.0 0.0 0.0 0.003502504290567756 0.0 10 0.0 0.0 0.0 0.003502504290567756 0.0 11 0.0 0.0 0.0 0.003502504290567756 0.0 12 0.0 0.0 0.0 0.003502504290567756 0.0 13 0.0 0.0 0.0 0.003502504290567756 0.0 14 0.0 0.0 0.0 0.003502504290567756 0.0 15 0.0 0.0 0.0 0.003502504290567756 0.0 16 0.0 0.0 0.0 0.0046700057207570075 0.0 17 0.0 0.0 0.0 0.0046700057207570075 0.0 18 0.0 0.0 0.0 0.0046700057207570075 0.0 19 0.0 0.0 0.0 0.009340011441514015 0.0 20 0.0 0.0 0.0 0.009340011441514015 0.0 21 0.0 0.0 0.0 0.009340011441514015 0.0 22 0.0 0.0 0.0 0.010507512871703269 0.0 23 0.0 0.0 0.0 0.014010017162271024 0.0 24 0.0 0.0 0.0 0.014010017162271024 0.0 25 0.0 0.0 0.0 0.014010017162271024 0.0 26 0.0 0.0 0.0 0.014010017162271024 0.0 27 0.0 0.0 0.0 0.02451753003397429 0.0 28 0.0 0.0 0.0 0.031522538615109806 0.0 29 0.0 0.0 0.0 0.04670005720757008 0.0 30 0.0 0.0 0.0 0.06888258438116587 0.0 31 0.0 0.0 0.0 0.08289260154343689 0.0 32 0.0 0.0 0.0 0.10624263014722193 0.0 33 0.0 0.0 0.0 0.13192766161138547 0.0 34 0.0 0.0 0.0 0.18096272167933405 0.0 35 0.0 0.0 0.0 0.2335002860378504 0.0 36 0.0 0.0 0.0 0.33857541475488306 0.0 37 0.0 0.0 0.0 0.5090306235625138 0.0 38 0.0 0.0 0.0 0.6806533338003339 0.0 39 0.0 0.0 0.0 0.9048136083966702 0.0 40 0.0 0.0 0.0 1.1534914130269809 0.0 41 0.0 0.0 0.0 1.4453667705742939 0.0 42 0.0 0.0 0.0 1.7956171996310695 0.0 43 0.0 0.0 0.0 2.1575426429897377 0.0 44 0.0 0.0 0.0 2.4949505563144316 0.0 45 0.0 0.0 0.0 2.8989060511599125 0.0 46 0.0 0.0 0.0 3.252658984507256 0.0 47 0.0 0.0 0.0 3.6566144793527373 0.0 48 0.0 0.0 0.0 4.005697406979324 0.0 49 0.0 0.0 0.0 4.3536128331757205 0.0 50 0.0 0.0 0.0 4.749395818009877 0.0 51 0.0 0.0 0.0 5.179036344319522 0.0 52 0.0 0.0 0.0 5.651874423546169 0.0 53 0.0 0.0 0.0 6.167910055689818 0.0 54 0.0 0.0 0.0 6.60222058772022 0.0 55 0.0 0.0 0.0 6.999171073984566 0.0 56 0.0 0.0 0.0 7.392619055958344 0.0 57 0.0 0.0 0.0 7.810584567966096 0.0 58 0.0 0.0 0.0 8.200530045649305 0.0 59 0.0 0.0 0.0 8.577633007600435 0.0 60 0.0 0.0 0.0 8.969913488144023 0.0 61 0.0 0.0 0.0 9.370366478698937 0.0 62 0.0 0.0 0.0 9.84904206507653 0.0 63 0.0 0.0 0.0 10.289190104257878 0.0 64 0.0 0.0 0.0 10.805225736401527 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAATAC 15 0.0022178618 70.0 3 CAATTCC 15 0.0022178618 70.0 70 ACGATCT 15 0.0022178618 70.0 61 CTTAATA 25 2.378522E-4 55.999996 2 CTATGGG 20 0.0069302004 52.5 6 CAGAACA 20 0.0069302004 52.5 51 TCGATTA 20 0.0069302004 52.5 44 ACAGGCT 20 0.0069302004 52.5 55 GTTGTCA 20 0.0069302004 52.5 22 ATCGATT 20 0.0069302004 52.5 43 TCTGCAA 30 5.848813E-4 46.666664 32 TGCAAAA 30 5.848813E-4 46.666664 59 GTTGATC 35 0.0012492745 40.0 29 TTGATCG 35 0.0012492745 40.0 30 GCAAGAG 35 0.0012492745 40.0 14 TATGGGT 35 0.0012492745 40.0 7 GCTGAGC 35 0.0012492745 40.0 9 TGTTGTC 35 0.0012492745 40.0 21 CAAGGTT 35 0.0012492745 40.0 36 GTGAGGT 35 0.0012492745 40.0 24 >>END_MODULE