FastQCFastQC Report
Wed 25 May 2016
SRR1780337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550828
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGATG502.375633E-630.7712144
AAGATGT300.005768630729.305926
ACAGTCG407.054507E-427.48178341
TATCAGG602.8882333E-421.979442
GTATCAG500.00259270921.9794391
GAAAATT654.937429E-420.30161326
TTGGTAA654.9495406E-420.29423933
ATACCAT654.958641E-420.2887126
TGGATTC550.00446478620.01219224
TATACCG550.004504875719.9813086
TGTATAA808.97909E-519.24948311
CTCTAAT809.04194E-519.2320081
AATACTG809.04194E-519.2320085
TTTCAGG708.1319996E-418.85149830
CAAAGTG600.007381106318.3445124
CCAAAGT600.007396605318.3378423
CGTATTC600.007419903318.32784529
TATCGGA600.007419903318.32784526
ATGCTGA600.007447160318.31628
TACGCAT600.007447160318.31625
Produced by FastQC (version 0.11.5)