FastQCFastQC Report
Wed 25 May 2016
SRR1780337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550828
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT176093.1968236908799117No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC57601.0456984757492358No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG46840.8503561910433021No Hit
CACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCTG32870.5967380016992601RNA PCR Primer, Index 34 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC9670.17555389341137342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT207.856177E-443.9962839
GCTTATA250.002346380135.21623
ATGCCGT24200.033.54261844
TATGGTC607.95626E-933.015193
CTTTATA1550.032.660192
TCTTCTG4100.032.19239844
TATCGTG353.217201E-431.42591542
GTCTTCT4150.031.27446243
CGTCTTC4300.030.18349342
ATATCGT300.005744708229.33085337
CGAGAAT300.005744708229.33085314
TCTTTAT1850.028.5926321
TATGCCG29150.027.6957743
CCGTCTT4750.027.32400341
AGCTATG609.7468E-625.66449512
TTAATAC450.00139350724.4556983
AGCTAGT450.001397198424.4445956
GCCGTCT5550.023.78177340
GAGCTAT651.8122533E-523.69030411
CGTGCGA651.8122533E-523.69030410
Produced by FastQC (version 0.11.5)