FastQCFastQC Report
Wed 25 May 2016
SRR1780336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117872
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTA150.002219939670.061
CTGACTT200.006936642352.50000419
ATCCATG200.006936642352.50000455
GAAAAGA200.006936642352.50000434
TGAATCC200.006936642352.50000445
TGCCGCT602.573397E-740.83333213
CTTTTCG350.001251189440.036
GGACTTT350.001251189440.033
TGGGAAA350.001251189440.028
TCTGCCG904.436515E-935.00000411
TTTGTTA400.00241066335.00000416
TTCCGAC806.170194E-835.00000419
TTCCAAA400.00241066335.00000411
CGAAGCG400.00241066335.00000446
GCCGTAT601.207324E-535.054
CTTCCGA957.521521E-933.15789418
CGACGAT552.9772957E-431.81818422
ACGATGG552.9772957E-431.81818424
AGGGTTC450.0042929431.11111315
GACTTTT450.0042929431.11111334