##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780336_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117872 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.76490599972852 34.0 31.0 34.0 12.0 34.0 2 29.804355572146058 34.0 31.0 34.0 12.0 34.0 3 29.702965929143478 34.0 31.0 34.0 11.0 34.0 4 29.623676530473734 34.0 31.0 34.0 11.0 34.0 5 29.637683249626715 34.0 31.0 34.0 11.0 34.0 6 32.319762114836436 37.0 31.0 38.0 11.0 38.0 7 32.73753732862767 37.0 33.0 38.0 12.0 38.0 8 32.6001170761504 37.0 32.0 38.0 11.0 38.0 9 32.86963825166282 38.0 34.0 38.0 12.0 38.0 10-11 32.631303447807795 38.0 32.5 38.0 11.0 38.0 12-13 32.67642442649654 38.0 32.5 38.0 11.0 38.0 14-15 32.592015067191525 38.0 32.5 38.0 11.0 38.0 16-17 32.63061201981811 38.0 32.5 38.0 11.0 38.0 18-19 32.6338782747387 38.0 32.5 38.0 11.0 38.0 20-21 32.65012895344102 38.0 32.5 38.0 11.0 38.0 22-23 32.62949216098819 38.0 32.5 38.0 11.0 38.0 24-25 32.649598717252616 38.0 32.5 38.0 11.0 38.0 26-27 32.610327304194385 38.0 32.5 38.0 11.0 38.0 28-29 32.67367144020632 38.0 34.0 38.0 11.0 38.0 30-31 32.71125033935116 38.0 34.0 38.0 11.0 38.0 32-33 32.75074233066377 38.0 34.0 38.0 11.0 38.0 34-35 32.72812033392154 38.0 34.0 38.0 11.0 38.0 36-37 32.66180687525451 38.0 33.5 38.0 11.0 38.0 38-39 32.62296389303651 38.0 33.5 38.0 11.0 38.0 40-41 32.68139167910954 38.0 34.0 38.0 11.0 38.0 42-43 32.7619154676259 38.0 34.0 38.0 11.0 38.0 44-45 32.832742296728654 38.0 34.0 38.0 11.0 38.0 46-47 32.70935845663092 38.0 34.0 38.0 11.0 38.0 48-49 32.66480589113615 38.0 34.0 38.0 11.0 38.0 50-51 32.607990023075885 38.0 33.5 38.0 11.0 38.0 52-53 32.65723836025519 38.0 33.0 38.0 11.0 38.0 54-55 32.63474362019818 38.0 33.0 38.0 11.0 38.0 56-57 32.549112596715084 38.0 32.0 38.0 11.0 38.0 58-59 32.604477738563865 38.0 32.0 38.0 11.0 38.0 60-61 32.64500899280576 38.0 32.5 38.0 11.0 38.0 62-63 32.49404862902131 38.0 32.0 38.0 11.0 38.0 64-65 32.46035105877562 38.0 32.0 38.0 11.0 38.0 66-67 32.40929567666622 38.0 31.5 38.0 11.0 38.0 68-69 32.37128834668115 38.0 31.0 38.0 11.0 38.0 70-71 32.25449640287769 38.0 31.0 38.0 11.0 38.0 72-73 32.28886419166554 38.0 31.0 38.0 11.0 38.0 74-75 32.26725176462604 38.0 31.0 38.0 11.0 38.0 76 30.85510553821094 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3968305996467585 1101 2 0.08284309141642865 1101 3 0.02222981323275164 1101 4 -0.17484897067442873 1101 5 -0.7448152339940854 1101 6 -0.2966073006318908 1101 7 -0.05101865630759761 1101 8 -0.47540636360344735 1101 9 -0.48969414523253363 1101 10-11 -0.7603190097929513 1101 12-13 -0.4917520704559877 1101 14-15 -0.345808203028966 1101 16-17 -0.8843911023027147 1101 18-19 -0.75473582443054 1101 20-21 -0.3263603224956242 1101 22-23 -0.9020048588260536 1101 24-25 -0.9595861618485486 1101 26-27 -0.6331469920356909 1101 28-29 -0.8947404002438049 1101 30-31 -0.5015492391531993 1101 32-33 -0.6010350949222243 1101 34-35 -0.8871318202307457 1101 36-37 -0.8473643816419312 1101 38-39 -0.5667440625739175 1101 40-41 -0.20844505869580843 1101 42-43 -1.0042110399910271 1101 44-45 -0.3626471278785459 1101 46-47 -0.8149625577080926 1101 48-49 -0.8551887669503699 1101 50-51 -0.9140343129906938 1101 52-53 -0.5296741618919043 1101 54-55 -0.7642203581139313 1101 56-57 -0.6848408130538317 1101 58-59 -0.9713110224216059 1101 60-61 -0.6427392004126204 1101 62-63 -0.32219587688272355 1101 64-65 -0.5452673226942331 1101 66-67 -0.4297524719469834 1101 68-69 -0.7536767786888099 1101 70-71 -0.9303381425776891 1101 72-73 -0.592154446264864 1101 74-75 -1.2327774183106008 1101 76 -1.8167749099701815 1102 1 -0.5411687176690876 1102 2 -0.2442564266847853 1102 3 -0.5558172104849 1102 4 -0.39820305480949614 1102 5 -1.2151467323391643 1102 6 -0.1827351324303521 1102 7 -1.1787990064988172 1102 8 -0.443825701292738 1102 9 -0.414699718794985 1102 10-11 -0.3433222167350394 1102 12-13 -0.6675851036744227 1102 14-15 -0.5904833072117128 1102 16-17 -0.4567145061201785 1102 18-19 -0.5071538930625366 1102 20-21 -0.29193714333560905 1102 22-23 -0.7725267157655686 1102 24-25 -0.8694402202594844 1102 26-27 -1.1254040275864448 1102 28-29 -0.6146946112846052 1102 30-31 -0.38513038164775537 1102 32-33 -0.3146011459246054 1102 34-35 -0.6291016029475571 1102 36-37 -0.4167595214954787 1102 38-39 -0.5682832232811634 1102 40-41 -0.6833425908938473 1102 42-43 -0.48295004292357646 1102 44-45 -0.7644452448719079 1102 46-47 -1.2383646602273437 1102 48-49 -1.026854490398776 1102 50-51 -0.7420427505067728 1102 52-53 -0.5650276972454407 1102 54-55 -0.9742664900615914 1102 56-57 -0.6842963188760613 1102 58-59 -0.8939971365338657 1102 60-61 -0.6058465043518453 1102 62-63 -0.6629763471199013 1102 64-65 -0.8095099184308836 1102 66-67 -0.8901587417760055 1102 68-69 -0.6705835372763822 1102 70-71 -0.7162018732631523 1102 72-73 -0.5369804653693251 1102 74-75 -0.9798663037696045 1102 76 -0.6555789092156061 1103 1 -0.16724662430006987 1103 2 0.017067787806244183 1103 3 0.09285956620258062 1103 4 -0.33313218554594215 1103 5 -0.39762269626026736 1103 6 -0.8890489395470098 1103 7 -0.6247797776356947 1103 8 -0.5335769154864138 1103 9 -0.23143081206487892 1103 10-11 -0.6248169139300046 1103 12-13 0.0329550886142016 1103 14-15 -0.6828859239229814 1103 16-17 -0.3407466203039391 1103 18-19 -0.24696953385944198 1103 20-21 -0.2556104508982955 1103 22-23 -0.14872851971045264 1103 24-25 -0.2511430649418145 1103 26-27 -0.8028497605629532 1103 28-29 -0.43546475698784803 1103 30-31 -0.3543790683362289 1103 32-33 -0.24231945299170832 1103 34-35 -0.6563342325441468 1103 36-37 -0.6073383697460457 1103 38-39 -0.48437996230386204 1103 40-41 -1.0217061551717777 1103 42-43 -0.46741331975973566 1103 44-45 -0.18720765938275719 1103 46-47 -0.049272579743650624 1103 48-49 -0.3233407625763931 1103 50-51 -0.5081741807773668 1103 52-53 -0.39600698161456194 1103 54-55 -0.06468674975035782 1103 56-57 0.07905981156303454 1103 58-59 -0.6118829527693705 1103 60-61 -0.17091737361540282 1103 62-63 -0.37194155675415885 1103 64-65 -0.4119977764941254 1103 66-67 -0.6203351715510088 1103 68-69 -0.618274091345171 1103 70-71 -0.29884138163860996 1103 72-73 -0.2791501551304485 1103 74-75 -0.2993231379454393 1103 76 -0.7357652507757209 1104 1 0.7196466730805149 1104 2 0.6807169917096836 1104 3 0.5971931563412554 1104 4 0.8528269824047499 1104 5 0.46715324107923095 1104 6 0.5169924061129763 1104 7 0.05207885102377929 1104 8 -0.18902439879406785 1104 9 0.6186269691369688 1104 10-11 0.47332285237713734 1104 12-13 0.3108505688401948 1104 14-15 0.27644253010885933 1104 16-17 0.6196411557617978 1104 18-19 0.6611824307014231 1104 20-21 0.20346922295891545 1104 22-23 0.092588249678343 1104 24-25 0.30924631615731357 1104 26-27 0.24506048597017838 1104 28-29 0.057055787439473704 1104 30-31 0.30091593679987483 1104 32-33 0.28409137135343343 1104 34-35 0.07937294516514726 1104 36-37 0.11836142480968448 1104 38-39 0.14549933918560498 1104 40-41 0.4547239295739871 1104 42-43 0.4090954116218768 1104 44-45 -0.239250866881477 1104 46-47 0.09742747161742216 1104 48-49 0.3314406318766103 1104 50-51 0.10026187469258474 1104 52-53 0.21322356538082232 1104 54-55 -0.0048270296681849345 1104 56-57 0.4488193775620104 1104 58-59 0.489924314821792 1104 60-61 0.37655148873694344 1104 62-63 0.250201483821094 1104 64-65 0.2641626626430096 1104 66-67 0.27891688289172833 1104 68-69 0.22387087527013705 1104 70-71 0.3142091448123381 1104 72-73 0.35405156546534045 1104 74-75 0.19464193652810735 1104 76 0.48555280263978773 1105 1 -0.1861229399595068 1105 2 -0.7123058018233124 1105 3 -0.31416716700886127 1105 4 -0.5152985145472755 1105 5 -0.5194704668454975 1105 6 0.11123107560675294 1105 7 -0.27376431091632014 1105 8 -0.6514962381791705 1105 9 -0.42760613449409135 1105 10-11 -0.32671607553169224 1105 12-13 -0.5872908305767197 1105 14-15 -0.23606409591817368 1105 16-17 -0.20645876472587332 1105 18-19 -0.1910447426354409 1105 20-21 -0.1527584141998446 1105 22-23 -0.07529042776665307 1105 24-25 -0.22442632497705972 1105 26-27 -0.33053419995614775 1105 28-29 -0.14531964325227875 1105 30-31 -0.09446323759715369 1105 32-33 -0.11147001878703833 1105 34-35 -0.009602174103335415 1105 36-37 -0.27000658473179584 1105 38-39 -0.5100466454420101 1105 40-41 -0.3770453852948208 1105 42-43 -0.3743494598331978 1105 44-45 -0.5079238393172076 1105 46-47 0.18326765184535532 1105 48-49 -0.21063878105739775 1105 50-51 -0.07396950617144427 1105 52-53 -0.3202917076114886 1105 54-55 -0.6189479215314222 1105 56-57 -0.6968745331191499 1105 58-59 -0.3652390167445958 1105 60-61 -0.23097650622728594 1105 62-63 -0.1300496426035025 1105 64-65 -0.5765849188571224 1105 66-67 0.04424785294208533 1105 68-69 -0.280394629633129 1105 70-71 -0.2525853489591938 1105 72-73 -0.2940815514177757 1105 74-75 0.08352346316473813 1105 76 -0.4807455673575589 1106 1 -0.25008120660656274 1106 2 -0.2035897253121668 1106 3 0.054183310104406246 1106 4 0.3460661245682246 1106 5 0.04276188688446325 1106 6 0.23817867533337278 1106 7 -0.5679221605758968 1106 8 0.20945314369311774 1106 9 0.06344420679545237 1106 10-11 -0.3091368522357598 1106 12-13 -0.030210262020332834 1106 14-15 -0.39858107388358377 1106 16-17 0.0783341689800352 1106 18-19 -0.3324571363339146 1106 20-21 0.11253905705656564 1106 22-23 -0.37864016952855906 1106 24-25 -0.09554950930802164 1106 26-27 -0.0696228833311423 1106 28-29 -0.19029719340756657 1106 30-31 0.08818073987514197 1106 32-33 -0.048636407173674456 1106 34-35 -0.3292248090835699 1106 36-37 0.2577767201967873 1106 38-39 -0.057528715845414524 1106 40-41 0.4136909177045496 1106 42-43 -0.20476013194434728 1106 44-45 -0.18116397046928512 1106 46-47 -0.20898607033024774 1106 48-49 -0.40907983615684174 1106 50-51 -0.18045703547735314 1106 52-53 -0.18734883143601166 1106 54-55 -0.10593220231452705 1106 56-57 -0.15292647487932243 1106 58-59 -0.5121578945119012 1106 60-61 0.06358362388665739 1106 62-63 -0.25487337455495407 1106 64-65 0.01403199768482466 1106 66-67 -0.011842154065384136 1106 68-69 -0.23786536697426897 1106 70-71 -0.10819694501770982 1106 72-73 -0.0030684102562688054 1106 74-75 -0.03836318891025314 1106 76 0.2677469623376574 1107 1 -0.06658941226271509 1107 2 -1.309973440307278 1107 3 -0.42786979091556887 1107 4 -1.1217125087163744 1107 5 -0.7002284843382114 1107 6 -1.2940167378043306 1107 7 -0.21253982112040148 1107 8 -0.32788107782931775 1107 9 -0.3459734814328712 1107 10-11 -0.03808633792236549 1107 12-13 -0.07854447489158645 1107 14-15 0.14002919854247153 1107 16-17 -0.17293106793287194 1107 18-19 -0.09191937820395424 1107 20-21 -0.23876424510363137 1107 22-23 0.01217312908465118 1107 24-25 -0.08594235996248756 1107 26-27 -0.20808522036430332 1107 28-29 0.09228968619378719 1107 30-31 -0.5055419547983249 1107 32-33 -0.3272134590202782 1107 34-35 -0.2180569845989595 1107 36-37 -0.33414652642276366 1107 38-39 -0.18497375914463277 1107 40-41 -0.038852965469743594 1107 42-43 -0.009917972952734999 1107 44-45 -0.39567894135802106 1107 46-47 -0.22052243561820006 1107 48-49 -0.1741956323693472 1107 50-51 -0.21828437497610764 1107 52-53 -0.47335293210129237 1107 54-55 0.05306374027615135 1107 56-57 -0.020489693469002646 1107 58-59 -0.5241311450244766 1107 60-61 -0.19744880943427034 1107 62-63 -0.26999133356560634 1107 64-65 0.1260389878484176 1107 66-67 -0.31872590799086 1107 68-69 -0.5121730552891037 1107 70-71 -0.18552995537319106 1107 72-73 -0.1905830091437224 1107 74-75 -0.1424240587011525 1107 76 -1.0113578122555182 1108 1 0.29977400057229886 1108 2 -0.05627643654880288 1108 3 0.4747260150488124 1108 4 0.5344397973006991 1108 5 0.3897641392172382 1108 6 0.26514410381286524 1108 7 0.41978832528445764 1108 8 0.1324090623231271 1108 9 -0.1608774120240426 1108 10-11 0.5302950593869866 1108 12-13 0.4079891230570638 1108 14-15 0.6003061664724996 1108 16-17 0.5150409367037554 1108 18-19 0.4002124142720689 1108 20-21 0.3560055044485111 1108 22-23 0.37650801966739067 1108 24-25 0.3025547772745085 1108 26-27 0.5857299821366624 1108 28-29 0.2875459298272105 1108 30-31 0.5275554493957131 1108 32-33 -0.09544381484590048 1108 34-35 0.36221453881246646 1108 36-37 0.047509836638816694 1108 38-39 0.18698345747695555 1108 40-41 0.5708760380077251 1108 42-43 0.5381342944258165 1108 44-45 0.42269806521053255 1108 46-47 0.5607352481782044 1108 48-49 0.36435068116466596 1108 50-51 0.20376680349872345 1108 52-53 -0.2064266262949701 1108 54-55 0.2177736001237136 1108 56-57 0.44113523188840276 1108 58-59 0.4519656620288046 1108 60-61 0.2471532138190895 1108 62-63 0.4335941453763965 1108 64-65 0.049126381153413945 1108 66-67 0.17154421038350876 1108 68-69 0.11903106146947806 1108 70-71 0.2711334328736754 1108 72-73 0.11168436940837978 1108 74-75 0.11222134507137582 1108 76 -0.7871402422671103 1109 1 -0.09483228836052149 1109 2 0.15598437138882915 1109 3 0.039126029969175136 1109 4 0.23371988980694525 1109 5 -0.27620624386307213 1109 6 0.17089403853628227 1109 7 -0.12651142479946742 1109 8 0.41186745316202433 1109 9 0.08409629083693915 1109 10-11 -0.09478420503913298 1109 12-13 0.0064775038247120165 1109 14-15 -0.010761944982611737 1109 16-17 -0.07719954223988168 1109 18-19 0.18144259816553898 1109 20-21 -0.04589311168786736 1109 22-23 0.04205876755056437 1109 24-25 -0.23184132105068045 1109 26-27 -0.1383039243872659 1109 28-29 -0.25994744926190094 1109 30-31 0.0025545168730545242 1109 32-33 0.17206896071454025 1109 34-35 -0.04327428956606383 1109 36-37 -0.22756103508336878 1109 38-39 0.1793087959826778 1109 40-41 -0.11566113234552233 1109 42-43 -0.05647945468828652 1109 44-45 0.19917704614976373 1109 46-47 0.25303540857210294 1109 48-49 -0.26146061354978656 1109 50-51 0.2971729357677404 1109 52-53 0.4274043632980735 1109 54-55 0.15542047244270663 1109 56-57 0.02459600998530931 1109 58-59 0.019573554900603085 1109 60-61 0.28880433844141606 1109 62-63 0.14636317816390232 1109 64-65 -0.026142185372748372 1109 66-67 0.15774287501105988 1109 68-69 1.2509705454277764E-4 1109 70-71 0.08599970912418797 1109 72-73 0.20304361443803742 1109 74-75 0.060037377000952574 1109 76 -0.6753587509768728 1110 1 0.46042855910493685 1110 2 -0.09862493502030034 1110 3 0.5689536719450565 1110 4 -0.060453207554544974 1110 5 -0.18027722025048476 1110 6 0.4715548213097236 1110 7 0.3286985932218798 1110 8 0.10428462427241669 1110 9 0.6688474576037251 1110 10-11 -0.038460560105903596 1110 12-13 0.3629028076461651 1110 14-15 0.32008228587547904 1110 16-17 0.6959301652597674 1110 18-19 0.6242548526824265 1110 20-21 0.29860575620178764 1110 22-23 0.2735499186064274 1110 24-25 0.7285348168357402 1110 26-27 0.49852893915877416 1110 28-29 0.5212179312658023 1110 30-31 0.30026719188807505 1110 32-33 0.29967396972521243 1110 34-35 0.18606816226148482 1110 36-37 0.2597962959087283 1110 38-39 0.34268387107163534 1110 40-41 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2116 8 0.052452873933205524 2116 9 -0.43432757631757823 2116 10-11 -0.06741578398650461 2116 12-13 -0.7957403402507133 2116 14-15 -0.6210574698911344 2116 16-17 -0.3953588442382028 2116 18-19 -0.46171529657130606 2116 20-21 -0.6518148679501792 2116 22-23 -0.5222981139580156 2116 24-25 -0.2133105020244983 2116 26-27 0.07676503142472058 2116 28-29 -0.388171485287792 2116 30-31 -0.5972090632001184 2116 32-33 -0.19698817410111502 2116 34-35 -0.040797509380304575 2116 36-37 -0.06413857519032007 2116 38-39 -0.14950066081439672 2116 40-41 -0.40340107042601403 2116 42-43 -0.7384045883781205 2116 44-45 -0.42851223051783904 2116 46-47 -0.1312088920189467 2116 48-49 -0.09577527721430101 2116 50-51 -0.1820108525801416 2116 52-53 -0.28240143461917455 2116 54-55 -0.3109065751227291 2116 56-57 -0.47316925880163296 2116 58-59 -0.18087113972366353 2116 60-61 -0.23089169308124013 2116 62-63 -0.4909348798152777 2116 64-65 -0.2962350646297125 2116 66-67 -0.3505149352900929 2116 68-69 -0.10487912472986238 2116 70-71 -0.05573403700584123 2116 72-73 -0.2753802527164808 2116 74-75 -0.4426307907446194 2116 76 -0.10228810645111963 2117 1 0.4582830367168782 2117 2 0.46090165080059364 2117 3 0.8770227017958021 2117 4 0.29352300513202323 2117 5 0.6869685819883209 2117 6 1.0343929742947964 2117 7 1.201226578296506 2117 8 0.9345551466604789 2117 9 0.5763684464096954 2117 10-11 0.9463342160134971 2117 12-13 1.0256943188401948 2117 14-15 1.1901499164724996 2117 16-17 0.822411042125438 2117 18-19 1.0628159534286965 2117 20-21 1.098710700231642 2117 22-23 0.5963169428601631 2117 24-25 0.9640758616118603 2117 26-27 0.6731499746065381 2117 28-29 1.007872548803114 2117 30-31 1.0812781527089683 2117 32-33 1.017578587262527 2117 34-35 1.126780615619694 2117 36-37 0.8491852884460442 2117 38-39 0.7681911005492452 2117 40-41 0.6890989295739871 2117 42-43 1.0014249570764235 2117 44-45 0.8395843603912496 2117 46-47 0.8697996307083287 2117 48-49 1.1773213136947902 2117 50-51 0.9676269315107717 2117 52-53 0.5844948721990022 2117 54-55 1.0718775157863618 2117 56-57 0.6654386957438234 2117 58-59 0.8098817011854322 2117 60-61 0.5214378523733032 2117 62-63 1.054711427002907 2117 64-65 0.46071806037028296 2117 66-67 0.8456782465280881 2117 68-69 0.9298367843610436 2117 70-71 1.0405940879941582 2117 72-73 0.2402376450107937 2117 74-75 1.1710268797099275 2117 76 1.1481948480943345 2118 1 0.718699703383546 2118 2 0.9189745674672594 2118 3 0.6093143684624671 2118 4 -0.21611241153464178 2118 5 0.6184789986549859 2118 6 0.4601742242947964 2118 7 0.6481015782965045 2118 8 0.3418468133271446 2118 9 0.30006636307636114 2118 10-11 -0.15158245065316578 2118 12-13 0.6087672355068605 2118 14-15 0.5503061664725024 2118 16-17 0.4726714587921066 2118 18-19 0.32661803676202794 2118 20-21 0.6734502835649749 2118 22-23 0.7191034011935002 2118 24-25 0.5703258616118632 2118 26-27 0.7607801829398753 2118 28-29 0.4934194238031111 2118 30-31 0.3345333610422969 2118 32-33 0.7697921289291898 2118 34-35 0.8217024906196926 2118 36-37 1.1090811217793757 2118 38-39 0.8964463088825809 2118 40-41 0.3307655962406528 2118 42-43 0.7774666237430949 2118 44-45 1.255730193724581 2118 46-47 0.9981850473749958 2118 48-49 0.7413838136947888 2118 50-51 0.546663389844106 2118 52-53 0.5544167471989994 2118 54-55 0.8354191824530304 2118 56-57 0.8784595290771549 2118 58-59 0.5158712845187665 2118 60-61 0.9349795190399703 2118 62-63 0.8912999686695784 2118 64-65 0.708764935370283 2118 66-67 0.7863032465280853 2118 68-69 0.7808784510277036 2118 70-71 0.5563492963274896 2118 72-73 0.7169303533441251 2118 74-75 1.0857404213765989 2118 76 0.7664240147610002 2119 1 -0.6115307930703544 2119 2 -0.4320006098377043 2119 3 -0.7464480428850493 2119 4 -0.2087542555062747 2119 5 0.3294719064564049 2119 6 0.43065294769905194 2119 7 0.18852711021139612 2119 8 -0.17694751291399058 2119 9 -0.47989817593072814 2119 10-11 -0.40699025207160844 2119 12-13 0.04781865394657814 2119 14-15 0.02849765583420094 2119 16-17 -0.4062292504277565 2119 18-19 -0.6008642327415146 2119 20-21 -0.2238458157258023 2119 22-23 -0.2800544002249481 2119 24-25 -0.16797201072856183 2119 26-27 -0.17015953337218548 2119 28-29 -0.45099546981391114 2119 30-31 -0.6054489084612484 2119 32-33 -0.5087096441204508 2119 34-35 -0.18973367959307552 2119 36-37 -0.4017788072986406 2119 38-39 -0.15696681168479643 2119 40-41 0.1829819082973927 2119 42-43 -0.35422663866825843 2119 44-45 -0.03504994811938644 2119 46-47 -0.18443906610018246 2119 48-49 0.19470828177989574 2119 50-51 -0.17164334774454204 2119 52-53 -0.29292367833291166 2119 54-55 0.12766209025444653 2119 56-57 -0.43479401702213494 2119 58-59 -0.3001748147720136 2119 60-61 -0.1530967220947801 2119 62-63 0.20109606796035706 2119 64-65 -0.15878825611908098 2119 66-67 -0.24827122155701886 2119 68-69 -0.1272775773410828 2119 70-71 -0.31913829232499324 2119 72-73 -0.2907380863721869 2119 74-75 -0.7754741176304982 2119 76 0.07821479490284489 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 16.0 13 274.0 14 1280.0 15 2375.0 16 3239.0 17 3623.0 18 3644.0 19 3118.0 20 2524.0 21 1808.0 22 1177.0 23 887.0 24 758.0 25 726.0 26 754.0 27 866.0 28 946.0 29 1263.0 30 1536.0 31 1900.0 32 2515.0 33 3475.0 34 4778.0 35 7867.0 36 15569.0 37 50954.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.29540518528573 19.499966064883942 11.557452151486359 28.647176598343965 2 19.444821501289532 19.189459752952356 30.831749694583955 30.533969051174154 3 18.380107234966744 21.02280439799104 29.3937491516221 31.20333921542012 4 14.967082937423646 21.39609067463011 29.414958599158407 34.22186778878783 5 15.453203474955885 31.195703814307045 32.474209311795846 20.876883398941224 6 27.981199945703818 29.994400705850417 17.480826659427176 24.543572689018596 7 26.80365141848785 27.906542690376 19.406644495724176 25.883161395411975 8 23.159019953848244 29.662684946382516 18.429313153250984 28.74898194651826 9 23.402504411565086 17.754004343694856 19.331987240396362 39.51150400434369 10-11 25.689307044930093 22.639388489208635 24.006549477399215 27.664754988462057 12-13 24.294997963893035 20.29913804805212 24.089690511741548 31.316173476313292 14-15 24.03921202660513 21.425783901180942 22.186778878783766 32.34822519343016 16-17 25.154404778064343 23.157747386996064 22.7764015202932 28.911446314646394 18-19 24.273788516356724 22.184657934030135 23.51873218406407 30.022821365549067 20-21 23.66762250576897 22.55242975430976 24.225855164924663 29.554092574996606 22-23 25.89673544183521 22.229197773856388 22.939290077372064 28.93477670693634 24-25 24.529150264693904 23.70240599972852 22.37808809556129 29.390355640016292 26-27 23.34863241482286 24.162226822315734 23.942921134790282 28.54621962807113 28-29 24.622047644902946 23.102602823401654 25.067021854214744 27.20832767748066 30-31 24.40189357947604 22.165993620198183 25.10265372607574 28.329459074250035 32-33 23.12126713723361 23.836025519207276 24.99448554364056 28.048221799918554 34-35 23.70707207818651 23.64514049138048 24.437525451337045 28.210261979095968 36-37 24.158087408745963 22.13639429378603 24.96786755068019 28.737650746787814 38-39 23.071212841047917 22.22113818379259 27.008534681688612 27.699114293470885 40-41 23.218830595900638 21.662481335686167 27.142578390118093 27.976109678295103 42-43 21.91444957241754 21.900875525994298 26.46557282475906 29.719102076829103 44-45 21.042741278675173 21.363852314374913 28.54155354961314 29.051852857336772 46-47 21.439782136554907 22.628783765440478 28.0617958463418 27.86963825166282 48-49 21.554313153250984 22.822638115922356 27.30207343559115 28.320975295235513 50-51 21.378274738699606 23.762216641780913 25.90988529930772 28.949623320211753 52-53 20.409002986290215 23.81354350481879 26.454119723089452 29.323333785801548 54-55 20.828950047509164 23.04533731505362 26.005752002171846 30.119960635265375 56-57 20.78441020768291 23.144173340572824 26.188577439934846 29.88283901180942 58-59 21.089402063255054 23.13484118365685 25.640101126645853 30.135655626442244 60-61 20.82513234695263 22.259739378308673 26.1554907017782 30.759637572960504 62-63 20.973174290756074 22.746708293742365 25.676157187457584 30.60396022804398 64-65 21.464809284647753 22.733982625220577 24.594899552056468 31.2063085380752 66-67 21.398635808334465 23.865294556807385 23.201014659970138 31.535054974888016 68-69 21.39142459617212 24.207615040043436 23.442802361884077 30.958158001900365 70-71 22.53249287362563 23.799969458395548 23.01140219899552 30.656135468983305 72-73 22.491346545405186 24.258093525179856 22.742466404235103 30.50809352517986 74-75 21.976381159223564 23.760095697027282 24.016305823265917 30.247217320483237 76 21.8313085380752 22.83918148500068 22.567700556535904 32.76180942038822 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.0 14 1.5 15 3.0 16 6.5 17 10.0 18 10.0 19 14.0 20 21.5 21 25.0 22 35.5 23 66.0 24 92.0 25 98.0 26 149.5 27 235.5 28 363.5 29 457.0 30 516.0 31 711.0 32 1068.5 33 1290.0 34 1469.5 35 1875.5 36 2274.5 37 2447.0 38 2864.5 39 3767.0 40 4516.0 41 4966.0 42 5152.0 43 5323.5 44 5736.5 45 6042.5 46 6107.0 47 6077.5 48 6035.0 49 5540.0 50 5058.0 51 4896.0 52 4340.5 53 3693.0 54 3439.0 55 3181.0 56 2643.5 57 2396.0 58 2428.0 59 2333.0 60 2298.5 61 2383.0 62 2407.0 63 2435.5 64 2560.0 65 2734.5 66 2849.5 67 2886.0 68 2901.5 69 2861.0 70 2718.0 71 2631.0 72 2595.0 73 2402.0 74 1974.0 75 1703.0 76 1456.0 77 1018.0 78 664.5 79 502.0 80 370.0 81 177.0 82 74.0 83 32.0 84 27.5 85 15.5 86 9.0 87 10.0 88 9.0 89 7.5 90 5.5 91 3.5 92 3.0 93 5.5 94 6.0 95 3.5 96 3.0 97 3.5 98 5.5 99 17.5 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.2418895072621145E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 117872.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.61924799782815 #Duplication Level Percentage of deduplicated Percentage of total 1 76.90499947484508 49.695432333378584 2 12.3201344396597 15.922356454459074 3 4.70276231488289 9.116668929007737 4 2.2214053145677974 5.741821637029998 5 1.130395966810209 3.652266865752681 6 0.7535973112068061 2.9218134926021446 7 0.5146518222875749 2.3279489615854487 8 0.36366978258586286 1.8800054296185693 9 0.27570633336834366 1.6034342337450793 >10 0.8126772397857368 7.138251662820687 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005090267408714538 0.0 2 0.0 0.0 0.0 0.00848377901452423 0.0 3 0.0 0.0 0.0 0.00848377901452423 0.0 4 0.0 0.0 0.0 0.01442242432469119 0.0 5 0.0 0.0 0.0 0.01696755802904846 0.0 6 0.0 0.0 0.0 0.01696755802904846 0.0 7 0.0 0.0 0.0 0.017815935930500883 0.0 8 0.0 0.0 0.0 0.017815935930500883 0.0 9 0.0 0.0 0.0 0.022057825437762996 0.0 10 0.0 0.0 0.0 0.02884484864938238 0.0 11 0.0 0.0 0.0 0.03054160445228723 0.0 12 0.0 0.0 0.0 0.03054160445228723 0.0 13 0.0 0.0 0.0 0.03054160445228723 0.0 14 0.0 0.0 0.0 0.03138998235373965 0.0 15 0.0 0.0 0.0 0.03138998235373965 0.0 16 0.0 0.0 0.0 0.03138998235373965 0.0 17 0.0 0.0 0.0 0.03308673815664449 0.0 18 0.0 0.0 0.0 0.0407221392697163 0.0 19 0.0 0.0 0.0 0.05768969729876476 0.0 20 0.0 0.0 0.0 0.05938645310166961 0.0 21 0.0 0.0 0.0 0.06362834260893173 0.0 22 0.0 0.0 0.0 0.06617347631328899 0.0 23 0.0 0.0 0.0 0.0738088774263608 0.0 24 0.0 0.0 0.0 0.07720238903217048 0.0 25 0.0 0.0 0.0 0.08229265644088503 0.0 26 0.0 0.0 0.0 0.09162481335686168 0.0 27 0.0 0.0 0.0 0.16034342337450794 0.0 28 0.0 0.0 0.0 0.2137912311660106 0.0 29 0.0 0.0 0.0 0.280813085380752 0.0 30 0.0 0.0 0.0 0.3580154744129225 0.0 31 0.0 0.0 0.0 0.4428532645581648 0.0 32 0.0 0.0 0.0 0.5412651011266458 0.0 33 0.0 0.0 0.0 0.6854893443735578 0.0 34 0.0 0.0 0.0 0.9035224650468304 0.0 35 0.0 0.0 0.0 1.169064748201439 0.0 36 0.0 0.0 0.0 1.5313221121216234 0.0 37 0.0 0.0 0.0 2.0327134518800056 0.0 38 0.0 0.0 0.0 2.5349531695398397 0.0 39 0.0 0.0 0.0 3.0541604452287228 0.0 40 0.0 0.0 0.0 3.602212569566988 0.0 41 0.0 0.0 0.0 4.261402198995521 0.0 42 0.0 0.0 0.0 4.940104520157459 0.0 43 0.0 0.0 0.0 5.67564816071671 0.0 44 0.0 0.0 0.0 6.3874372200352925 0.0 45 0.0 0.0 0.0 6.959243925614226 0.0 46 0.0 0.0 0.0 7.595527351703542 0.0 47 0.0 0.0 0.0 8.2632007601466 0.0 48 0.0 0.0 0.0 8.832462332021176 0.0 49 0.0 0.0 0.0 9.346579340301345 0.0 50 0.0 0.0 0.0 9.874270395004752 0.0 51 0.0 0.0 0.0 10.509705443192615 0.0 52 0.0 0.0 0.0 11.202830188679245 0.0 53 0.0 0.0 0.0 11.94346409664721 0.0 54 0.0 0.0 0.0 12.614531016696077 0.0 55 0.0 0.0 0.0 13.22112121623456 0.0 56 0.0 0.0 0.0 13.738631736120537 0.0 57 0.0 0.0 0.0 14.262080901316683 0.0 58 0.0 0.0 0.0 14.830494095289806 0.0 59 0.0 0.0 0.0 15.312372743314782 0.0 60 0.0 0.0 0.0 15.792554635536854 0.0 61 0.0 0.0 0.0 16.29903624270395 0.0 62 0.0 0.0 0.0 16.797882448757974 0.0 63 0.0 0.0 0.0 17.407017782000814 0.0 64 0.0 0.0 0.0 18.022940138455272 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAGTA 15 0.0022199396 70.0 61 CTGACTT 20 0.0069366423 52.500004 19 ATCCATG 20 0.0069366423 52.500004 55 GAAAAGA 20 0.0069366423 52.500004 34 TGAATCC 20 0.0069366423 52.500004 45 TGCCGCT 60 2.573397E-7 40.833332 13 CTTTTCG 35 0.0012511894 40.0 36 GGACTTT 35 0.0012511894 40.0 33 TGGGAAA 35 0.0012511894 40.0 28 TCTGCCG 90 4.436515E-9 35.000004 11 TTTGTTA 40 0.002410663 35.000004 16 TTCCGAC 80 6.170194E-8 35.000004 19 TTCCAAA 40 0.002410663 35.000004 11 CGAAGCG 40 0.002410663 35.000004 46 GCCGTAT 60 1.207324E-5 35.0 54 CTTCCGA 95 7.521521E-9 33.157894 18 CGACGAT 55 2.9772957E-4 31.818184 22 ACGATGG 55 2.9772957E-4 31.818184 24 AGGGTTC 45 0.00429294 31.111113 15 GACTTTT 45 0.00429294 31.111113 34 >>END_MODULE