FastQCFastQC Report
Wed 25 May 2016
SRR1780336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117872
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT103398.771379123116601No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG27272.3135265372607576RNA PCR Primer, Index 34 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG26272.2286887471155152No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC24552.0827677480656983No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC7470.6337382923849599No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT4840.4106149043029727RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3120.26469390525315595No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT2420.20530745215148635No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1720.14592099904981676No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1680.14252748744400706No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1650.1399823537396498No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT1230.10435048187864802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT653.6379788E-1253.8461531
CTTTATA707.2759576E-1250.02
CTTATAT305.857832E-446.6666641
AACTCGT602.573397E-740.83333237
CAACTCG602.573397E-740.83333236
ATATACA350.001251189440.04
GTTATGA350.001251189440.02
AATAACA350.001251189440.069
TTTATAC901.03682396E-1038.888893
TTAATAC400.00241066335.0000042
TGGCTTG400.00241066335.00000457
ATACCTT400.00241066335.00000422
CTTAATA501.7012036E-435.01
CCGTCTT24050.034.63617347
TATGCCG24800.034.43548243
TCGTATG24600.034.43089340
GCCGTCT24400.034.4262346
CTTGAAA23900.034.41422757
CGTATGC24850.034.366241
TGCCGTC24850.034.366245