##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780336_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117872 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.605258246233205 34.0 34.0 34.0 34.0 34.0 2 33.655533120673276 34.0 34.0 34.0 34.0 34.0 3 33.689239174697974 34.0 34.0 34.0 34.0 34.0 4 33.7061303787159 34.0 34.0 34.0 34.0 34.0 5 33.67542758246233 34.0 34.0 34.0 34.0 34.0 6 37.3795557893308 38.0 38.0 38.0 37.0 38.0 7 37.39280745215149 38.0 38.0 38.0 37.0 38.0 8 37.27031865073979 38.0 38.0 38.0 36.0 38.0 9 37.30714673544183 38.0 38.0 38.0 36.0 38.0 10-11 37.25075081444278 38.0 38.0 38.0 36.0 38.0 12-13 37.2324767544455 38.0 38.0 38.0 36.0 38.0 14-15 37.14199300936609 38.0 38.0 38.0 36.0 38.0 16-17 37.15414178091489 38.0 38.0 38.0 36.0 38.0 18-19 37.08791316003801 38.0 38.0 38.0 35.5 38.0 20-21 37.12810082122981 38.0 38.0 38.0 36.0 38.0 22-23 37.130293878105064 38.0 38.0 38.0 36.0 38.0 24-25 37.112821535224654 38.0 38.0 38.0 35.5 38.0 26-27 36.96894088502783 38.0 38.0 38.0 35.0 38.0 28-29 36.932842405321026 38.0 38.0 38.0 35.0 38.0 30-31 36.89905999728519 38.0 37.0 38.0 35.0 38.0 32-33 36.81617347631329 38.0 37.0 38.0 34.5 38.0 34-35 36.91008891000408 38.0 37.0 38.0 35.0 38.0 36-37 36.89802921813492 38.0 37.5 38.0 35.0 38.0 38-39 35.957394461789065 38.0 37.0 38.0 33.0 38.0 40-41 36.381943973123384 38.0 37.0 38.0 34.0 38.0 42-43 36.755166621419846 38.0 37.0 38.0 34.0 38.0 44-45 36.8551988597801 38.0 37.0 38.0 34.5 38.0 46-47 36.77205782543776 38.0 37.0 38.0 34.5 38.0 48-49 36.80790179177413 38.0 37.0 38.0 34.5 38.0 50-51 36.56381074385774 38.0 37.0 38.0 34.0 38.0 52-53 36.571170422152846 38.0 37.0 38.0 34.0 38.0 54-55 36.53257771141577 38.0 37.0 38.0 34.0 38.0 56-57 36.59674901588163 38.0 37.0 38.0 34.0 38.0 58-59 36.43006396769377 38.0 37.0 38.0 34.0 38.0 60-61 36.423226041808064 38.0 37.0 38.0 34.0 38.0 62-63 36.446038923578115 38.0 37.0 38.0 34.0 38.0 64-65 36.34433113886249 38.0 37.0 38.0 34.0 38.0 66-67 35.90382788109135 38.0 37.0 38.0 32.0 38.0 68-69 35.7617457920456 38.0 37.0 38.0 31.0 38.0 70-71 34.81801869824895 38.0 36.0 38.0 26.0 38.0 72-73 33.311409834396635 38.0 34.0 38.0 16.0 38.0 74-75 32.90056586806027 38.0 34.0 38.0 11.0 38.0 76 32.173069091896295 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16151664829727252 1101 2 0.16170448033826545 1101 3 0.04955343848912719 1101 4 0.06537904370144076 1101 5 0.0953242555821916 1101 6 -0.15668418794878392 1101 7 -0.14987256224876688 1101 8 -0.04591764272484511 1101 9 0.09753413810848599 1101 10-11 0.1585289047375582 1101 12-13 -0.00810889153746075 1101 14-15 0.23054573641523035 1101 16-17 0.09962492384101296 1101 18-19 0.10430725087044124 1101 20-21 0.10304260250662622 1101 22-23 -0.10129195671297708 1101 24-25 -0.05364032544784436 1101 26-27 0.05763447913969344 1101 28-29 -0.027703453498915565 1101 30-31 -0.041908261479051134 1101 32-33 0.0691109887140513 1101 34-35 0.05158646989638527 1101 36-37 0.2557692295534153 1101 38-39 0.07053906234222751 1101 40-41 0.47037794427436097 1101 42-43 0.23663066716196113 1101 44-45 0.38690856620852543 1101 46-47 0.12968649465680215 1101 48-49 -0.09905225840862641 1101 50-51 0.1224319844694648 1101 52-53 0.21703522462548364 1101 54-55 0.2251965608419937 1101 56-57 0.060864376403742426 1101 58-59 0.30551237133371956 1101 60-61 0.2892880760751808 1101 62-63 0.3834564969082308 1101 64-65 0.06445972527070154 1101 66-67 -0.002017308489378422 1101 68-69 -0.26063094667622266 1101 70-71 -0.021559119071064003 1101 72-73 -0.3338892651943368 1101 74-75 -0.3786087990255709 1101 76 -1.2482265762824127 1102 1 0.039352734112981125 1102 2 0.10085832781129511 1102 3 0.04156180740751125 1102 4 -0.10935642644998467 1102 5 -0.0384069443629258 1102 6 0.12336588836614482 1102 7 0.28214712528874486 1102 8 0.0937987058200278 1102 9 0.06717965953669136 1102 10-11 0.06675376797090138 1102 12-13 0.3363629919836626 1102 14-15 0.18855966174664474 1102 16-17 -0.007751900428850433 1102 18-19 0.21940989016663082 1102 20-21 0.10101468324611318 1102 22-23 -0.026803438251626233 1102 24-25 0.18020063080397364 1102 26-27 0.27117480600769284 1102 28-29 0.2124570650996418 1102 30-31 0.18089275376679126 1102 32-33 -0.09133901214341478 1102 34-35 0.19655054185536613 1102 36-37 0.2788223413162001 1102 38-39 0.3718286989459614 1102 40-41 0.3393692152180918 1102 42-43 0.30843101358030367 1102 44-45 0.20057151145041985 1102 46-47 0.2982395978448764 1102 48-49 0.3652512177794094 1102 50-51 0.3895085223439665 1102 52-53 0.37428257075229254 1102 54-55 0.41449617840986264 1102 56-57 0.3441728430738351 1102 58-59 0.2699401808379278 1102 60-61 0.15280439375109012 1102 62-63 0.45121386687272746 1102 64-65 0.1655984469527212 1102 66-67 -0.226953426888997 1102 68-69 0.04930628643588619 1102 70-71 -0.06763533110652986 1102 72-73 -0.14494121079636813 1102 74-75 -0.4105710359962913 1102 76 -0.3966634063830803 1103 1 0.07919811026523149 1103 2 0.16587963814928486 1103 3 0.033929732870888074 1103 4 0.04313638750580395 1103 5 0.24908438282509593 1103 6 0.12609162927208217 1103 7 0.2166797846206947 1103 8 0.24272327713531894 1103 9 -0.06618340915198218 1103 10-11 0.2488332604875083 1103 12-13 0.15297018793965833 1103 14-15 0.1806302336566432 1103 16-17 0.20584706874550562 1103 18-19 0.414325144403918 1103 20-21 0.2470648368787849 1103 22-23 0.19157796723950327 1103 24-25 0.29849778612164357 1103 26-27 0.47401948833498153 1103 28-29 0.21312611059472886 1103 30-31 0.3919146588118849 1103 32-33 0.3209169101481777 1103 34-35 0.38212393862412597 1103 36-37 0.35886000609963276 1103 38-39 0.31215975256871786 1103 40-41 0.6865790477089249 1103 42-43 0.28387456596409066 1103 44-45 0.2133081553199787 1103 46-47 0.5191980674652541 1103 48-49 0.30573094817885504 1103 50-51 0.23096951946954647 1103 52-53 0.5591869220259511 1103 54-55 0.1902420402395819 1103 56-57 -0.020803864231147884 1103 58-59 0.6525350861803858 1103 60-61 0.6189308681291195 1103 62-63 0.36297419990871305 1103 64-65 0.23017372696065053 1103 66-67 -0.041751722061960095 1103 68-69 0.3932700093023769 1103 70-71 0.35596958513888666 1103 72-73 0.18465042365700413 1103 74-75 -0.09500457747711977 1103 76 -0.11531738596480423 1104 1 0.17691254272542523 1104 2 0.12220667975175559 1104 3 0.20562015403164935 1104 4 0.19221188827621916 1104 5 0.09789023798688845 1104 6 0.046578275394324464 1104 7 0.07245148467205098 1104 8 0.02116446871210087 1104 9 0.3327797392815057 1104 10-11 0.20182985781673324 1104 12-13 0.2259911817763225 1104 14-15 0.08437638157652572 1104 16-17 0.1665237508204953 1104 18-19 0.26202352653027106 1104 20-21 0.16091466995531079 1104 22-23 0.19622453888818825 1104 24-25 0.1912735308571314 1104 26-27 0.36683535890509233 1104 28-29 0.40122799811399545 1104 30-31 0.2526298616881064 1104 32-33 0.1994720590952781 1104 34-35 0.3295831707761536 1104 36-37 0.33027436151822087 1104 38-39 0.32892432627185286 1104 40-41 0.44096078884914647 1104 42-43 0.42161050587163373 1104 44-45 0.4153176571176189 1104 46-47 0.3203023304339254 1104 48-49 0.4100661433509103 1104 50-51 0.13590339209410018 1104 52-53 0.1291503272647887 1104 54-55 0.09634758723495906 1104 56-57 0.14270088666766867 1104 58-59 0.494936858137244 1104 60-61 0.42863008487282883 1104 62-63 0.12892020658564718 1104 64-65 0.15674344961087883 1104 66-67 0.20193247156927185 1104 68-69 0.495005050391228 1104 70-71 0.6038990920726306 1104 72-73 0.5519703389112394 1104 74-75 0.40195388425888723 1104 76 0.26194571110761444 1105 1 0.08408190397641846 1105 2 0.046197867135795434 1105 3 0.001546803912376049 1105 4 0.23004650264145 1105 5 0.24263579854877548 1105 6 0.1977013869766182 1105 7 0.07607923447326925 1105 8 -0.06467571151583229 1105 9 0.06907214585452692 1105 10-11 0.11982863200173455 1105 12-13 0.29690209484284935 1105 14-15 0.24594376827145226 1105 16-17 0.10561399730869425 1105 18-19 0.2041037677176547 1105 20-21 0.27246797634280284 1105 22-23 0.16969972441695091 1105 24-25 0.2960600071359636 1105 26-27 0.5111667927773453 1105 28-29 0.4308090675547547 1105 30-31 0.3965637339383079 1105 32-33 0.32349057884083976 1105 34-35 0.19198129561049626 1105 36-37 0.19386931248562433 1105 38-39 0.07952480997182221 1105 40-41 0.03565499770151348 1105 42-43 0.4337485591448953 1105 44-45 0.12465041619527284 1105 46-47 0.11449793075197334 1105 48-49 0.28311560664271695 1105 50-51 -0.038849788398870544 1105 52-53 0.39941768745561745 1105 54-55 -0.00663743794394378 1105 56-57 0.2351588113960048 1105 58-59 0.4342838631730217 1105 60-61 0.5316937941227664 1105 62-63 0.31886769532140846 1105 64-65 0.32737192827507755 1105 66-67 -0.05844968453992294 1105 68-69 0.3311972383053572 1105 70-71 -0.25192684219717876 1105 72-73 0.0895523359957835 1105 74-75 0.07660353307945655 1105 76 -0.10983238324701006 1106 1 0.15593870943705213 1106 2 -0.009697488038916902 1106 3 0.04045121688570674 1106 4 0.1040678364823222 1106 5 0.1307672274662508 1106 6 0.22578315804875615 1106 7 0.14685464086304023 1106 8 0.33648008781110406 1106 9 0.1109612202634338 1106 10-11 0.21356862906032603 1106 12-13 0.22560761689906883 1106 14-15 0.2704179920432068 1106 16-17 0.24173618675251163 1106 18-19 0.36258496435060295 1106 20-21 0.4851618711962047 1106 22-23 0.37229767754478615 1106 24-25 0.41551567409965173 1106 26-27 0.33916810775860995 1106 28-29 0.4187666075016381 1106 30-31 0.3963950356832555 1106 32-33 0.5690979358150017 1106 34-35 0.4957679225970324 1106 36-37 0.34023018833235597 1106 38-39 0.1813277185027644 1106 40-41 0.5007041797312581 1106 42-43 0.5712808929771143 1106 44-45 0.3703183315173959 1106 46-47 0.4306341351277396 1106 48-49 0.459798912436419 1106 50-51 0.6005901333943413 1106 52-53 0.5950341281819718 1106 54-55 0.5576538996169376 1106 56-57 0.5220929152622205 1106 58-59 0.5419003731089092 1106 60-61 0.5896135283581287 1106 62-63 0.6647856301087174 1106 64-65 0.5500449737543889 1106 66-67 0.580215955518554 1106 68-69 0.8165377925007462 1106 70-71 0.3509401630194873 1106 72-73 0.13649370686376727 1106 74-75 0.21256134986450093 1106 76 0.5709472622271221 1107 1 0.1074055191367691 1107 2 0.11616871261684736 1107 3 0.08609490886864535 1107 4 0.11342842683969678 1107 5 0.1697495011726815 1107 6 0.2443486172973195 1107 7 0.3063999341817265 1107 8 0.2093767666182771 1107 9 0.3868564024142884 1107 10-11 0.11108227631660128 1107 12-13 0.190488100673754 1107 14-15 0.3931740889711648 1107 16-17 0.32573061538449366 1107 18-19 0.16911834497712874 1107 20-21 0.2957915915031535 1107 22-23 0.2411282958455203 1107 24-25 0.3412495115091332 1107 26-27 0.2531493000659708 1107 28-29 0.3069023620153999 1107 30-31 0.36886694093539063 1107 32-33 0.27975880041518764 1107 34-35 0.326092082782516 1107 36-37 0.346930536975421 1107 38-39 0.47894171959281806 1107 40-41 0.5079582046985962 1107 42-43 0.4191712996696779 1107 44-45 0.3680906494314229 1107 46-47 0.3185795634050308 1107 48-49 0.4478386387126889 1107 50-51 0.4203338850705123 1107 52-53 0.4096217690882682 1107 54-55 0.40152582736217113 1107 56-57 0.12291391343681823 1107 58-59 0.24769494189022367 1107 60-61 0.2139095375629978 1107 62-63 0.24598139794530738 1107 64-65 0.7282465638435909 1107 66-67 0.9255153300373351 1107 68-69 0.6591855764977694 1107 70-71 0.29326266217599795 1107 72-73 -0.14805699345027534 1107 74-75 0.16698254182580996 1107 76 -0.3780539575910318 1108 1 -0.07938537622310093 1108 2 0.08726007515153356 1108 3 0.018222837492764654 1108 4 0.19741451697282741 1108 5 -0.2190593786615267 1108 6 -0.09943760631433207 1108 7 -0.03468159309355201 1108 8 0.16177235480191854 1108 9 0.15304945450603924 1108 10-11 0.10125140765244112 1108 12-13 -0.13697773936277713 1108 14-15 0.164982898553518 1108 16-17 0.22765463800559615 1108 18-19 0.22543398655217572 1108 20-21 0.13690385396407834 1108 22-23 0.03944400767241518 1108 24-25 -0.06798509455359181 1108 26-27 0.1426431354658746 1108 28-29 0.2728565578073159 1108 30-31 0.0701955791032276 1108 32-33 -0.21894593104710935 1108 34-35 0.15490148950561178 1108 36-37 0.415535862065866 1108 38-39 0.22002851575706472 1108 40-41 0.19400706486228358 1108 42-43 0.24415637115094313 1108 44-45 0.18674427267073668 1108 46-47 0.2417152548588959 1108 48-49 0.35086912801684633 1108 50-51 0.3601982989944261 1108 52-53 0.35707338312458603 1108 54-55 0.20060552808928378 1108 56-57 0.5216370860105002 1108 58-59 0.3371123784001142 1108 60-61 0.4838566730436966 1108 62-63 0.4043685910325294 1108 64-65 0.5846254320862343 1108 66-67 0.4749506807697088 1108 68-69 0.47736060898925814 1108 70-71 0.9447739551613523 1108 72-73 0.8285140136100324 1108 74-75 0.6255471208415813 1108 76 1.6881847582050966 1109 1 0.09353230067645057 1109 2 -0.04086853837377191 1109 3 0.009914060589487406 1109 4 -0.03161304839701273 1109 5 0.13015170716786173 1109 6 -0.014233010842659155 1109 7 0.07454478610745952 1109 8 0.07143888469859405 1109 9 0.05995571732822924 1109 10-11 -0.1635523895798272 1109 12-13 0.1650523624686997 1109 14-15 -0.1441716764026566 1109 16-17 0.05631758701525058 1109 18-19 0.1768402307239043 1109 20-21 0.08596480364514747 1109 22-23 0.19731076883471133 1109 24-25 0.045353709579345036 1109 26-27 0.018766825271541165 1109 28-29 -0.1358401485226466 1109 30-31 0.1560389525971999 1109 32-33 -0.15927846862776818 1109 34-35 -0.14469472818247908 1109 36-37 0.15231735335611063 1109 38-39 0.020263511467454975 1109 40-41 -0.0033380431238398955 1109 42-43 -0.0745239227802088 1109 44-45 0.11010023522062795 1109 46-47 0.2794917478276773 1109 48-49 -0.1493686652615267 1109 50-51 0.10192682299756228 1109 52-53 0.29284122228308007 1109 54-55 -0.18139305447627407 1109 56-57 0.09632282587397611 1109 58-59 0.0438479895754611 1109 60-61 0.3069151608649534 1109 62-63 -0.09832173684244339 1109 64-65 0.12758675104628736 1109 66-67 0.1178178149410698 1109 68-69 0.2796984432986349 1109 70-71 0.5949893678958773 1109 72-73 -0.05885589947884995 1109 74-75 -0.1781148956454146 1109 76 0.4043341856361806 1110 1 -0.2961539430276261 1110 2 -0.01782893761595261 1110 3 -0.014927675004983598 1110 4 -0.17773383763965 1110 5 0.06970163554455411 1110 6 -0.012393909137564663 1110 7 -0.1394379318815453 1110 8 -0.012180171722086186 1110 9 0.053615899390052846 1110 10-11 0.029641085767885045 1110 12-13 -0.05693368253846387 1110 14-15 -0.29585597934613617 1110 16-17 -0.11280121525820874 1110 18-19 -0.018649811325907706 1110 20-21 0.19307630496209782 1110 22-23 -0.07248729964641143 1110 24-25 -0.12182433210080035 1110 26-27 -0.11754388125773119 1110 28-29 0.02510435496609631 1110 30-31 -0.3245580623281725 1110 32-33 -0.2928490060336131 1110 34-35 -0.38562629326727205 1110 36-37 -0.17946953264731036 1110 38-39 -0.3197485027647815 1110 40-41 -0.2853536616257557 1110 42-43 -0.48078518612293664 1110 44-45 -0.1731999819597263 1110 46-47 -0.2803760956176333 1110 48-49 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-0.09449348233199117 2116 4 -0.057788111723780844 2116 5 0.028344783441433208 2116 6 -0.11773990642385712 2116 7 0.14995148467205155 2116 8 0.12616446871210485 2116 9 -0.058242987991221185 2116 10-11 -0.110386051274169 2116 12-13 0.019457090867234683 2116 14-15 0.036762745212890025 2116 16-17 -0.5523398855431481 2116 18-19 -0.16149920074246182 2116 20-21 -0.3668694209537833 2116 22-23 -0.3298550065663548 2116 24-25 -0.21821510550650203 2116 26-27 -0.3661191865494544 2116 28-29 -0.15644245643146348 2116 30-31 -0.040324683766442604 2116 32-33 -0.4411529409047219 2116 34-35 -0.12706455649657755 2116 36-37 -0.4309756384817831 2116 38-39 -0.7065870373645069 2116 40-41 -0.5000619384235847 2116 42-43 -0.5931054032192762 2116 44-45 -0.48036416106420177 2116 46-47 -0.45242494229334795 2116 48-49 -0.31817249301273165 2116 50-51 -0.24102842608771624 2116 52-53 -0.08619058182612349 2116 54-55 -0.23251604912868373 2116 56-57 -0.41309456787778487 2116 58-59 0.08527776722814906 2116 60-61 -0.1608585514908043 2116 62-63 -0.10715933886889673 2116 64-65 -0.35882473220730304 2116 66-67 -0.38107889206709444 2116 68-69 -0.5766426768814981 2116 70-71 -0.3723509079273697 2116 72-73 -0.5700182974523997 2116 74-75 -0.7715120248320204 2116 76 -0.3627133798014732 2117 1 -0.20447950272912152 2117 2 0.04763281611538872 2117 3 -0.1249906414228974 2117 4 0.05584825191258602 2117 5 -0.12902737564947842 2117 6 0.026691911757957598 2117 7 -0.3210144244188555 2117 8 -0.8840201903788056 2117 9 -0.33838503344576765 2117 10-11 -0.1758334944559934 2117 12-13 -0.452630977314584 2117 14-15 -0.24587930024166127 2117 16-17 -0.4162177264522384 2117 18-19 -0.4094963598333692 2117 20-21 -0.40495890959014247 2117 22-23 -0.19653114292999163 2117 24-25 -0.7636270373246816 2117 26-27 -0.28657373200400116 2117 28-29 -1.576399842795098 2117 30-31 -0.3420079224028001 2117 32-33 -0.6485393045410817 2117 34-35 -0.45468529513293987 2117 36-37 -0.20576967257268564 2117 38-39 -0.24973334418269388 2117 40-41 -0.6881230179690405 2117 42-43 -0.15882273276472603 2117 44-45 -0.4888016610642012 2117 46-47 -0.3384334650206213 2117 48-49 -0.6451540271036365 2117 50-51 -0.6077045624513531 2117 52-53 -0.568825525007945 2117 54-55 -0.551912355946861 2117 56-57 -0.6433431474232378 2117 58-59 -0.5320517782263963 2117 60-61 -0.45063127876353093 2117 62-63 -0.2462573502325327 2117 64-65 -0.5062537094800277 2117 66-67 -0.4848573011580015 2117 68-69 -0.5140503473360454 2117 70-71 -0.03743613520009603 2117 72-73 -0.04959216108876063 2117 74-75 0.5301499069861606 2117 76 -0.29877872071056544 2118 1 0.0231246639375442 2118 2 -0.4351796838846127 2118 3 -0.396084391422896 2118 4 -0.17331841475407828 2118 5 -0.13241279231614556 2118 6 -0.05455808824203956 2118 7 -0.07830609108552267 2118 8 -0.20042644037880564 2118 9 -0.3378642001124348 2118 10-11 -0.21606786945599055 2118 12-13 -0.3174747273145897 2118 14-15 -0.11410846690832699 2118 16-17 -0.11074897645223558 2118 18-19 -0.3493401098333635 2118 20-21 -0.38008911792347533 2118 22-23 -0.4574686429299888 2118 24-25 -0.402949953991353 2118 26-27 -0.47824039867066404 2118 28-29 -0.2698894261284295 2118 30-31 -0.7043777140694658 2118 32-33 -0.5568726378744131 2118 34-35 -0.7748675867996084 2118 36-37 -0.5079832142393528 2118 38-39 -0.15090521918269673 2118 40-41 0.10080927369762804 2118 42-43 -0.30765085776472034 2118 44-45 -0.37369749439753264 2118 46-47 -0.07098554835395277 2118 48-49 -0.05726340210363645 2118 50-51 -0.5019753957846902 2118 52-53 -0.7647890666746093 2118 54-55 -0.3551675642801939 2118 56-57 -0.6788900224232393 2118 58-59 -0.8080934448930677 2118 60-61 -0.44229794543019807 2118 62-63 -0.15393964189919984 2118 64-65 -0.4731807928133591 2118 66-67 -0.7010031344913301 2118 68-69 -0.8225138890027139 2118 70-71 -1.154623635200096 2118 72-73 -0.6344879944220949 2118 74-75 0.27572282365282774 2118 76 -0.5636224707105661 2119 1 -0.010031364431249301 2119 2 -0.1029988328207807 2119 3 -0.10060566801864468 2119 4 -0.20479004595975425 2119 5 -0.6067035724579881 2119 6 -0.1838134073909785 2119 7 -0.3267989988869431 2119 8 -0.2246285680383835 2119 9 -0.2539102994032163 2119 10-11 -0.16473808222195174 2119 12-13 -0.5263843017826701 2119 14-15 -1.192521587475703 2119 16-17 -0.5083553594309649 2119 18-19 -0.37101564174826507 2119 20-21 -0.49901642288801185 2119 22-23 -0.31770800463211657 2119 24-25 -0.29705456392042606 2119 26-27 -0.37114820008910954 2119 28-29 -0.42472098641211886 2119 30-31 -0.5992801963389667 2119 32-33 -0.6303212194346983 2119 34-35 -0.5664456009840038 2119 36-37 -0.8643218667216246 2119 38-39 -0.9798945808848245 2119 40-41 -1.0175861163732947 2119 42-43 -0.7055231981902566 2119 44-45 -0.6701513951067568 2119 46-47 -0.7698330660844519 2119 48-49 -0.9157740404015087 2119 50-51 -0.6544133390470961 2119 52-53 -0.8958617617100728 2119 54-55 -1.0513555075426027 2119 56-57 -0.8391293841253713 2119 58-59 -0.5452831612051199 2119 60-61 -1.0370674489762948 2119 62-63 -1.0094804220410438 2119 64-65 -1.1609910765013112 2119 66-67 -1.1578293756260862 2119 68-69 -1.4025227542509384 2119 70-71 -0.9370704437107378 2119 72-73 -0.907271682365355 2119 74-75 -0.8033108642904381 2119 76 -1.7838352366680112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 1.0 21 3.0 22 8.0 23 12.0 24 40.0 25 82.0 26 180.0 27 308.0 28 554.0 29 911.0 30 1390.0 31 2107.0 32 3262.0 33 5408.0 34 8895.0 35 13649.0 36 18270.0 37 62790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.44329442106692 15.392968643952761 12.851228451201301 37.31250848377902 2 18.67025247726347 18.11032306230487 37.388014117008275 25.83141034342337 3 17.8922899416316 17.23055517849871 34.447536310574186 30.429618569295506 4 14.306196552192208 25.546355368535362 31.326354011130718 28.82109406814171 5 15.01459209990498 28.688747115515135 40.43623591692684 15.860424867653048 6 27.45859915840912 38.537566173476314 16.21165331885435 17.792181349260215 7 26.175851771413054 23.955646803312067 21.777012352382243 28.091489072892628 8 22.303006651282747 37.99121080494095 15.271650604045064 24.434131939731234 9 20.958327677480657 16.601058775621013 15.73571331613954 46.70490023075879 10-11 25.44115650875526 27.18330052938781 23.264218813628343 24.111324148228586 12-13 24.277606216913263 17.90713655490702 26.393460703135606 31.421796525044115 14-15 21.50213791231166 17.04688136283426 19.871555585720102 41.579425139133974 16-17 28.360424867653045 25.03138998235374 17.924104112936064 28.684081037057148 18-19 31.15370910818515 23.19295506990634 18.401316682503055 27.25201913940546 20-21 24.31281389982354 20.294471969594134 18.545116736799237 36.84759739378309 22-23 23.88056535903353 25.155677344916523 18.098445771684542 32.86531152436542 24-25 29.569787566173478 25.675732998506856 19.29974888014117 25.4547305551785 26-27 29.529489615854487 28.350244332835615 20.13624949097326 21.984016560336634 28-29 29.774246640423513 23.292639473326997 19.159342337450795 27.7737715487987 30-31 29.906169404099366 19.767205103841455 25.516662141984526 24.809963350074657 32-33 24.573690104520157 25.457699877833583 26.916061490430298 23.05254852721596 34-35 26.703967015067192 26.78838061626171 23.830511062847833 22.677141305823266 36-37 26.06386588842134 24.97115515135062 20.447604180806298 28.517374779421743 38-39 20.982082258721324 23.589571738835346 26.79940952898059 28.62893647346274 40-41 22.213502782679516 18.41404235102484 28.337942853264558 31.034512013031083 42-43 28.108880819872407 18.05475430975974 27.11373354146871 26.722631328899144 44-45 21.757923849599567 24.534664721053346 30.091539975566718 23.615871453780372 46-47 26.43927310981404 20.005175105198862 26.569923306637712 26.985628478349394 48-49 26.099921949233067 18.29866295642731 22.47607574317904 33.125339351160584 50-51 23.28797339486901 18.75890796796525 27.633789195059048 30.319329442106692 52-53 19.040569431247455 18.844594136011946 36.736884077643545 25.377952355097054 54-55 20.00644767205104 20.087467761639743 30.623472919777385 29.28261164653183 56-57 24.75821229808606 21.427056468033122 24.35735373964979 29.45737749423103 58-59 20.531169404099362 21.6111544726483 31.830714673544186 26.026961449708157 60-61 24.9206766662142 26.73450861951948 24.95079408171576 23.394020632550564 62-63 19.814290077372064 36.72076489751595 20.00856861680467 23.456376408307317 64-65 18.834837790145244 38.705969186914615 19.078746436812814 23.380446586127324 66-67 18.933673815664452 37.93945975295235 18.894224243246914 24.232642188136282 68-69 18.30417741278675 38.10786276639066 19.51481267815936 24.073147142663228 70-71 19.048204832360526 36.24185557214606 19.68745758110493 25.02248201438849 72-73 19.785021039771955 32.75162888557079 20.712298086059455 26.7510519885978 74-75 19.957665942717522 31.685642052395817 21.394393918827205 26.962298086059455 76 20.171032984932808 31.633466811456497 21.10933894393919 27.08616125967151 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.5 17 1.0 18 7.0 19 10.0 20 11.5 21 16.0 22 22.5 23 35.0 24 49.5 25 58.0 26 112.0 27 201.0 28 263.5 29 291.0 30 362.5 31 563.5 32 859.5 33 1026.0 34 1129.5 35 1438.0 36 1812.5 37 1982.0 38 2248.0 39 3041.5 40 6182.0 41 10057.5 42 11320.0 43 10601.5 44 8754.5 45 6869.0 46 6112.0 47 5662.5 48 4911.5 49 4324.5 50 4039.0 51 3694.0 52 3032.0 53 2510.5 54 2306.0 55 2262.0 56 1954.5 57 1679.0 58 1667.0 59 1642.5 60 1722.5 61 1905.0 62 1983.0 63 2059.5 64 2223.5 65 2455.0 66 2674.0 67 2749.0 68 2819.5 69 2808.5 70 2706.0 71 2685.0 72 2509.5 73 2221.0 74 1886.5 75 1665.0 76 1433.5 77 978.0 78 592.0 79 430.0 80 330.5 81 165.5 82 57.0 83 14.0 84 11.5 85 5.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 117872.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.42714130582327 #Duplication Level Percentage of deduplicated Percentage of total 1 60.985849153201855 25.264693905253154 2 20.54432635006451 17.021854214741413 3 7.923245479306179 9.847122302158272 4 3.630890213184248 6.016696077100583 5 1.9352460527124162 4.008585584362699 6 1.2573979644078557 3.125424188950726 7 0.845774200815056 2.4526605130989547 8 0.7003747619340173 2.3211619383738293 9 0.43210255780139667 1.6110696348581512 >10 1.6956441604718313 10.057520021718474 >50 0.020478794208597 0.6490090946111036 >100 0.0184309147877373 1.5941020768291028 >500 0.0020478794208597 0.6337382923849599 >1k 0.0061436382625791 6.624983032441971 >5k 0.0 0.0 >10k+ 0.0020478794208597 8.771379123116601 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 10339 8.771379123116601 No Hit CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG 2727 2.3135265372607576 RNA PCR Primer, Index 34 (96% over 25bp) TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 2627 2.2286887471155152 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 2455 2.0827677480656983 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 747 0.6337382923849599 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 484 0.4106149043029727 RNA PCR Primer, Index 24 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT 312 0.26469390525315595 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT 242 0.20530745215148635 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 172 0.14592099904981676 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 168 0.14252748744400706 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 165 0.1399823537396498 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT 123 0.10435048187864802 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004241889507262115 0.0 2 0.0 0.0 0.0 0.007635401113071807 0.0 3 0.0 0.0 0.0 0.007635401113071807 0.0 4 0.0 0.0 0.0 0.013574046423238768 0.0 5 0.0 0.0 0.0 0.016119180127596035 0.0 6 0.0 0.0 0.0 0.016119180127596035 0.0 7 0.0 0.0 0.0 0.017815935930500883 0.0 8 0.0 0.0 0.0 0.017815935930500883 0.0 9 0.0 0.0 0.0 0.022057825437762996 0.0 10 0.0 0.0 0.0 0.029693226550834805 0.0 11 0.0 0.0 0.0 0.03138998235373965 0.0 12 0.0 0.0 0.0 0.03138998235373965 0.0 13 0.0 0.0 0.0 0.03138998235373965 0.0 14 0.0 0.0 0.0 0.03223836025519207 0.0 15 0.0 0.0 0.0 0.03223836025519207 0.0 16 0.0 0.0 0.0 0.03223836025519207 0.0 17 0.0 0.0 0.0 0.03393511605809692 0.0 18 0.0 0.0 0.0 0.041570517171168726 0.0 19 0.0 0.0 0.0 0.06108320890457446 0.0 20 0.0 0.0 0.0 0.0627799647074793 0.0 21 0.0 0.0 0.0 0.06702185421474141 0.0 22 0.0 0.0 0.0 0.06956698791909868 0.0 23 0.0 0.0 0.0 0.07805076693362291 0.0 24 0.0 0.0 0.0 0.07889914483507533 0.0 25 0.0 0.0 0.0 0.08398941224378988 0.0 26 0.0 0.0 0.0 0.09416994706121895 0.0 27 0.0 0.0 0.0 0.16204017917741279 0.0 28 0.0 0.0 0.0 0.2171847427718203 0.0 29 0.0 0.0 0.0 0.2833582190851093 0.0 30 0.0 0.0 0.0 0.36140898601873217 0.0 31 0.0 0.0 0.0 0.4445500203610696 0.0 32 0.0 0.0 0.0 0.5438102348310031 0.0 33 0.0 0.0 0.0 0.6863377222750102 0.0 34 0.0 0.0 0.0 0.8958870639337586 0.0 35 0.0 0.0 0.0 1.1597325912854621 0.0 36 0.0 0.0 0.0 1.514354554092575 0.0 37 0.0 0.0 0.0 2.0072621148364327 0.0 38 0.0 0.0 0.0 2.495079408171576 0.0 39 0.0 0.0 0.0 3.0117415501561013 0.0 40 0.0 0.0 0.0 3.5597936744943666 0.0 41 0.0 0.0 0.0 4.21558979231709 0.0 42 0.0 0.0 0.0 4.881566444957242 0.0 43 0.0 0.0 0.0 5.615413329713587 0.0 44 0.0 0.0 0.0 6.318718610017647 0.0 45 0.0 0.0 0.0 6.895615583005294 0.0 46 0.0 0.0 0.0 7.529353875390254 0.0 47 0.0 0.0 0.0 8.196178905931859 0.0 48 0.0 0.0 0.0 8.761198588299171 0.0 49 0.0 0.0 0.0 9.283799375593864 0.0 50 0.0 0.0 0.0 9.797916383874032 0.0 51 0.0 0.0 0.0 10.429957920456088 0.0 52 0.0 0.0 0.0 11.12393104384417 0.0 53 0.0 0.0 0.0 11.863716573910683 0.0 54 0.0 0.0 0.0 12.532238360255192 0.0 55 0.0 0.0 0.0 13.132041536582054 0.0 56 0.0 0.0 0.0 13.649552056468034 0.0 57 0.0 0.0 0.0 14.17045608795982 0.0 58 0.0 0.0 0.0 14.738020904031492 0.0 59 0.0 0.0 0.0 15.214809284647753 0.0 60 0.0 0.0 0.0 15.690749287362562 0.0 61 0.0 0.0 0.0 16.185353603909327 0.0 62 0.0 0.0 0.0 16.685896565766257 0.0 63 0.0 0.0 0.0 17.28400298629021 0.0 64 0.0 0.0 0.0 17.900773720646125 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTTAT 65 3.6379788E-12 53.846153 1 CTTTATA 70 7.2759576E-12 50.0 2 CTTATAT 30 5.857832E-4 46.666664 1 AACTCGT 60 2.573397E-7 40.833332 37 CAACTCG 60 2.573397E-7 40.833332 36 ATATACA 35 0.0012511894 40.0 4 GTTATGA 35 0.0012511894 40.0 2 AATAACA 35 0.0012511894 40.0 69 TTTATAC 90 1.03682396E-10 38.88889 3 TTAATAC 40 0.002410663 35.000004 2 TGGCTTG 40 0.002410663 35.000004 57 ATACCTT 40 0.002410663 35.000004 22 CTTAATA 50 1.7012036E-4 35.0 1 CCGTCTT 2405 0.0 34.636173 47 TATGCCG 2480 0.0 34.435482 43 TCGTATG 2460 0.0 34.430893 40 GCCGTCT 2440 0.0 34.42623 46 CTTGAAA 2390 0.0 34.414227 57 CGTATGC 2485 0.0 34.3662 41 TGCCGTC 2485 0.0 34.3662 45 >>END_MODULE