FastQCFastQC Report
Wed 25 May 2016
SRR1780335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372666
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACC357.343084E-637.6646042
CTAGCCT401.833171E-532.9565284
AAATTCA300.00568670829.38933219
TACGCAA300.00574305529.3301110
ACGCAAA407.0274435E-427.49697711
GATTGCG407.077264E-427.4637722
AGCTAGT407.077264E-427.4637726
TATACAA551.6050205E-423.9683842
TCATAGT500.002548725622.04199619
TGGATTC500.002586819221.98576728
GCGATTT500.002592876621.97691344
CTATACA500.002596921321.9710181
AGTGCGC550.00451218219.9736548
TGTAGGG550.00451218219.9736543
ATTGCGC550.00451218219.9736543
AAACTCG809.011981E-519.23754727
CGAAACT809.011981E-519.23754725
TGTGATA708.1811135E-418.8323024
AGCTTTG600.00735017618.35597635
TGCAATG600.00735017618.35597636
Produced by FastQC (version 0.11.5)