FastQCFastQC Report
Wed 25 May 2016
SRR1780335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372666
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT215895.793123064620867No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC60631.6269259873452366No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG58251.5630618301642758No Hit
CACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCTG37020.9933828146383088TruSeq Adapter, Index 2 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC12530.33622600398211805No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT6220.16690548641410807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT1700.038.872491
CTTTATA1800.036.6685682
TCTTCTG4150.034.98507344
ATGCCGT31050.034.29025344
GTCTTCT4250.034.16189643
TTTATAC2350.033.7038763
TCTGACC401.8160676E-533.0017133
CGTCTTC4450.032.62652642
CCGTCTT4900.029.63021341
GGTGCTA300.005704726629.3703271
TCCAATA300.005734761729.33879313
TGAGCTA300.005734761729.33879310
GTGCTAA300.005738524729.3348562
ACGGAAC300.005742289629.3309229
TAATGGT300.005742289629.330926
ATACAAT300.005742289629.330926
TATGCCG37000.028.71655543
CAGCAAA554.9521423E-627.99769420
GCCCTAA450.001386183524.4752711
CTTATAT551.5771906E-424.0302661
Produced by FastQC (version 0.11.5)