FastQCFastQC Report
Wed 25 May 2016
SRR1780334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100806
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAA150.002219004870.011
GTACAAG200.006933744552.51
GGTCATT305.8537733E-446.66666451
CCAAACG305.8537733E-446.66666466
GTGAATG350.001250327440.012
CTTATAA400.002409013235.01
ATTTCAT400.002409013235.017
GTAGAGT552.9742572E-431.8181846
GAATAAA450.004290017831.1111137
CTTAGGG450.004290017831.1111133
CGACGAA755.538389E-528.022
GAGTGCT500.00717969227.99999833
AAGGGTG500.00717969227.99999832
CTGATCC500.00717969227.99999860
TTTATAC808.5939166E-526.253
ACTATCC700.00121880625.08
GTCCGGT750.001821508523.33333468
TACTATC850.003767176520.5882367
TCCGGTG850.003767176520.58823669
AGTCCGG1205.6516947E-520.41666670
Produced by FastQC (version 0.11.5)