FastQCFastQC Report
Wed 25 May 2016
SRR1780334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100806
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT89578.885383806519453No Hit
CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG34773.4491994524135468TruSeq Adapter, Index 12 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG21572.1397535860960657No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC20111.9949209372457988No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6840.6785310398190584No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT4810.47715413765053666TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT2490.24700910660079758No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1840.18252881772910343No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1790.17756879550820387No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT1760.17459278217566415No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1400.1388806221851874No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT1380.13689661329682756No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1180.11705652441322938RNA PCR Primer, Index 12 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1160.11507251552486954RNA PCR Primer, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA150.002219004870.028
TCTTTAT1000.059.4999961
CTTTATA1000.059.4999962
AAATAAC252.3805512E-455.99999669
GTACCCT200.006933744552.547
ATGATTG200.006933744552.55
GACACAT200.006933744552.526
CACATGT200.006933744552.528
GCTATGG200.006933744552.51
ATACTGG200.006933744552.56
TAACACA200.006933744552.53
TAGGGCA200.006933744552.55
CTTCTCT200.006933744552.532
GCTGTAA200.006933744552.529
GATTCAG200.006933744552.511
TAAGAAT200.006933744552.512
ACCTTAG200.006933744552.517
AATAACA200.006933744552.570
TTTATAC1400.050.03
AAACTCG451.6704198E-646.66666836
Produced by FastQC (version 0.11.5)