##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780334_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 100806 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.545394123365675 34.0 34.0 34.0 34.0 34.0 2 33.612165942503424 34.0 34.0 34.0 34.0 34.0 3 33.636589091919134 34.0 34.0 34.0 34.0 34.0 4 33.66645834573339 34.0 34.0 34.0 34.0 34.0 5 33.6275618514771 34.0 34.0 34.0 34.0 34.0 6 37.28441759419082 38.0 38.0 38.0 36.0 38.0 7 37.292661151121955 38.0 38.0 38.0 36.0 38.0 8 37.14368192369502 38.0 38.0 38.0 36.0 38.0 9 37.21976866456362 38.0 38.0 38.0 36.0 38.0 10-11 37.124065035811356 38.0 38.0 38.0 36.0 38.0 12-13 37.1065363172827 38.0 38.0 38.0 36.0 38.0 14-15 37.02601531654862 38.0 38.0 38.0 35.5 38.0 16-17 37.03658016387914 38.0 38.0 38.0 35.5 38.0 18-19 36.95347003154574 38.0 37.5 38.0 35.0 38.0 20-21 37.01996408943912 38.0 37.5 38.0 35.0 38.0 22-23 36.996389103823184 38.0 37.5 38.0 35.0 38.0 24-25 36.999910719600024 38.0 37.5 38.0 35.0 38.0 26-27 36.86017201357062 38.0 37.0 38.0 35.0 38.0 28-29 36.8092127452731 38.0 37.0 38.0 34.5 38.0 30-31 36.76740967799536 38.0 37.0 38.0 34.0 38.0 32-33 36.64458464773922 38.0 37.0 38.0 34.0 38.0 34-35 36.66415689542289 38.0 37.0 38.0 34.0 38.0 36-37 36.69612919865881 38.0 37.0 38.0 34.0 38.0 38-39 35.849607166240105 38.0 37.0 38.0 31.5 38.0 40-41 36.24644862408984 38.0 37.0 38.0 34.0 38.0 42-43 36.59809931948495 38.0 37.0 38.0 34.0 38.0 44-45 36.714982243120446 38.0 37.0 38.0 34.0 38.0 46-47 36.61437315239172 38.0 37.0 38.0 34.0 38.0 48-49 36.639634545562764 38.0 37.0 38.0 34.0 38.0 50-51 36.37723945003273 38.0 37.0 38.0 34.0 38.0 52-53 36.379937702120905 38.0 37.0 38.0 34.0 38.0 54-55 36.35058925063984 38.0 37.0 38.0 34.0 38.0 56-57 36.40432613138107 38.0 37.0 38.0 34.0 38.0 58-59 36.217462254230895 38.0 37.0 38.0 34.0 38.0 60-61 36.2119318294546 38.0 37.0 38.0 34.0 38.0 62-63 36.20692220701149 38.0 37.0 38.0 34.0 38.0 64-65 36.020182330416844 38.0 37.0 38.0 32.5 38.0 66-67 35.451922504612824 38.0 36.5 38.0 29.5 38.0 68-69 35.26305973850763 38.0 36.0 38.0 28.0 38.0 70-71 34.28722000674563 38.0 35.0 38.0 23.0 38.0 72-73 32.8224560046029 38.0 32.5 38.0 15.0 38.0 74-75 32.35257821955042 38.0 31.0 38.0 11.0 38.0 76 31.66755947066643 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13428710759301055 1101 2 0.09649437961649454 1101 3 0.12921370388509956 1101 4 0.027265905999620088 1101 5 0.19533174739197534 1101 6 -0.0983545597633082 1101 7 0.13261904345741726 1101 8 0.19499342273370246 1101 9 0.07719648687471903 1101 10-11 0.046619003301103135 1101 12-13 0.1175842918451977 1101 14-15 0.06888507235917984 1101 16-17 0.22810033920159611 1101 18-19 0.34948548314199 1101 20-21 0.3629258704109972 1101 22-23 0.278399642129024 1101 24-25 0.269770547524665 1101 26-27 -0.15413483292358165 1101 28-29 -0.0696847588426408 1101 30-31 0.11199749726396391 1101 32-33 0.2253356141526126 1101 34-35 0.1845672287349558 1101 36-37 0.27601378649499964 1101 38-39 0.27405033289685576 1101 40-41 0.2033985049293321 1101 42-43 0.1565856290797356 1101 44-45 0.14251735890575645 1101 46-47 0.29687511238462605 1101 48-49 0.40695218352141893 1101 50-51 0.3615952828105051 1101 52-53 0.5574993114573914 1101 54-55 0.2693785735969172 1101 56-57 0.23735980977325255 1101 58-59 0.34762142665879026 1101 60-61 0.24754413508803452 1101 62-63 0.37381734645205 1101 64-65 0.20683010711108096 1101 66-67 0.42823959376187304 1101 68-69 0.035473432377195024 1101 70-71 0.46722452270584824 1101 72-73 -0.2100974398303208 1101 74-75 0.046138536253124585 1101 76 -0.37966604237509927 1102 1 -0.21097141819478793 1102 2 0.021670390716757026 1102 3 -0.2145141341332959 1102 4 -0.04568748317762328 1102 5 -0.03514258325193964 1102 6 0.13824547107075347 1102 7 0.18845952857998327 1102 8 0.19645290163806095 1102 9 0.056424607538680505 1102 10-11 0.0013186281533563715 1102 12-13 0.341501245439936 1102 14-15 0.2604519565772776 1102 16-17 0.21973916956286388 1102 18-19 0.23494208420063245 1102 20-21 0.3560139016561763 1102 22-23 0.3192033833284711 1102 24-25 0.30102600772303134 1102 26-27 0.4255969107425912 1102 28-29 0.28031267165224705 1102 30-31 0.2754848296037551 1102 32-33 0.2713708677627693 1102 34-35 0.4587950924484048 1102 36-37 0.36439448437258193 1102 38-39 0.24934197172722605 1102 40-41 0.6736641917580428 1102 42-43 0.423254008749808 1102 44-45 0.25517474109394556 1102 46-47 0.36069648038914437 1102 48-49 0.5033548763627778 1102 50-51 0.2258586056945191 1102 52-53 0.371816593948779 1102 54-55 0.1284977472440758 1102 56-57 0.461091758999828 1102 58-59 0.36286373099098057 1102 60-61 0.5324971131444656 1102 62-63 0.4493402407426075 1102 64-65 0.30450727448863546 1102 66-67 0.21243199830475845 1102 68-69 0.12216339589021885 1102 70-71 -0.05886623221474707 1102 72-73 -0.8235719246445541 1102 74-75 -1.000477425512642 1102 76 -1.3515453784149791 1103 1 -0.05480247971891572 1103 2 0.14556995705325448 1103 3 -0.17776791241406187 1103 4 0.07104111157234172 1103 5 0.05928188618629804 1103 6 0.028026836559952528 1103 7 0.4011048759614724 1103 8 0.06394015713897971 1103 9 0.09613536499759334 1103 10-11 0.07340892630953277 1103 12-13 0.19436112104961722 1103 14-15 0.23270951063499012 1103 16-17 -0.01889594215806767 1103 18-19 0.4299704776425983 1103 20-21 0.2728666864168545 1103 22-23 0.19627530336612864 1103 24-25 0.28979883014132923 1103 26-27 0.3245222665369525 1103 28-29 0.2500764101721771 1103 30-31 0.3865798049159821 1103 32-33 0.5002059854928973 1103 34-35 0.4303065049094741 1103 36-37 0.53378364979568 1103 38-39 0.026205573037223928 1103 40-41 0.22213835723122344 1103 42-43 0.3502272778515305 1103 44-45 0.3060275458852786 1103 46-47 0.41209011264650286 1103 48-49 0.34990509820219273 1103 50-51 0.3564834617242383 1103 52-53 0.2683933563953218 1103 54-55 0.09992903711758316 1103 56-57 0.14972036076052575 1103 58-59 0.42085245373678504 1103 60-61 0.37150579642989356 1103 62-63 0.345230953533175 1103 64-65 0.5124604428362076 1103 66-67 0.4960759735869402 1103 68-69 0.7746867566321569 1103 70-71 0.4652656896256673 1103 72-73 0.10788820936048182 1103 74-75 -0.006797094606355358 1103 76 0.777742431934854 1104 1 0.0698885806222691 1104 2 -0.09898969090623666 1104 3 0.12013029322420721 1104 4 0.012769566170767632 1104 5 0.013890361682108221 1104 6 -0.005097522955388456 1104 7 0.026278183574952152 1104 8 -0.07004421001194316 1104 9 -0.0403104726056398 1104 10-11 0.14784077874186607 1104 12-13 0.00787710553733234 1104 14-15 0.24699106266745474 1104 16-17 0.08048718320613091 1104 18-19 0.04838227662585837 1104 20-21 0.034721931860623556 1104 22-23 -0.10924341385612735 1104 24-25 0.025075218109257946 1104 26-27 0.12285248535665261 1104 28-29 0.011725695843402661 1104 30-31 -0.048271602643247036 1104 32-33 -0.16304978640414447 1104 34-35 0.17450949082383005 1104 36-37 0.20874396486389912 1104 38-39 -0.23773438717737605 1104 40-41 -0.08463118885353538 1104 42-43 -0.07064398222040325 1104 44-45 -0.2278352547657576 1104 46-47 -0.056187058973250714 1104 48-49 0.14511075657018324 1104 50-51 -0.20947389957943585 1104 52-53 -0.1433152055031286 1104 54-55 -0.30884083046535693 1104 56-57 -0.4843795447993813 1104 58-59 0.1349551724057818 1104 60-61 0.0943994809999893 1104 62-63 0.16968289960185956 1104 64-65 -0.45252137242290047 1104 66-67 -0.4836997814314472 1104 68-69 -0.35805682847443876 1104 70-71 0.040742411147959956 1104 72-73 0.11041498413091233 1104 74-75 5.82293341480522E-4 1104 76 0.026582024435910512 1105 1 -0.16199149509367317 1105 2 -0.04855310300857951 1105 3 0.044933370710928955 1105 4 0.02192875283499518 1105 5 0.058465070114664286 1105 6 0.02552469112533373 1105 7 0.20549293597160556 1105 8 -0.11571802084420568 1105 9 0.14827718081073726 1105 10-11 0.05290580896718211 1105 12-13 0.27054731375617536 1105 14-15 0.11221363090340475 1105 16-17 0.14912002194338214 1105 18-19 -0.008618395047832905 1105 20-21 0.09320333871169595 1105 22-23 0.18826223430898636 1105 24-25 0.09544830231271817 1105 26-27 0.20641479835658316 1105 28-29 0.2880583575030542 1105 30-31 -0.05152311390904174 1105 32-33 0.2342909964010076 1105 34-35 -0.041296212296394685 1105 36-37 0.3062435069045648 1105 38-39 0.12020662766050094 1105 40-41 0.0899123783443585 1105 42-43 0.0475400563703019 1105 44-45 0.030346951987617388 1105 46-47 -0.4441244101364674 1105 48-49 -0.10090499723087731 1105 50-51 -0.04228821233513003 1105 52-53 0.18049257369941074 1105 54-55 0.0549162679043107 1105 56-57 0.12096636715030229 1105 58-59 0.37338406658664525 1105 60-61 0.449485882660845 1105 62-63 0.49040708596077565 1105 64-65 0.15773996970325044 1105 66-67 -0.35293712648854125 1105 68-69 0.10727565000147621 1105 70-71 0.3950350166312617 1105 72-73 0.02457813977602541 1105 74-75 0.12369702741976596 1105 76 0.13209585480777264 1106 1 0.05650133893314546 1106 2 -0.11318555793229734 1106 3 0.028875426497641854 1106 4 0.12370098276256414 1106 5 0.11841267375159248 1106 6 0.13084138090804487 1106 7 0.03831771636920678 1106 8 0.307613771419625 1106 9 0.16421183946384588 1106 10-11 0.1818779156091921 1106 12-13 0.16572376621417817 1106 14-15 0.19815907704302305 1106 16-17 0.3098073389413827 1106 18-19 0.29588152789869326 1106 20-21 0.4427333125135178 1106 22-23 0.2097293324751135 1106 24-25 0.2565517080763158 1106 26-27 0.19038767553335134 1106 28-29 0.12112970902100528 1106 30-31 0.06177631589524424 1106 32-33 0.20126587696812237 1106 34-35 0.4314576789040956 1106 36-37 0.37554540242005885 1106 38-39 0.48565584942041085 1106 40-41 0.3115952262408044 1106 42-43 0.35900561346692683 1106 44-45 0.40473437686048186 1106 46-47 0.40267787064640004 1106 48-49 0.3170484624701544 1106 50-51 0.3139762351914541 1106 52-53 0.5662685021937861 1106 54-55 0.4783559350332993 1106 56-57 0.462236208420137 1106 58-59 0.6219123452120101 1106 60-61 0.5840520715959698 1106 62-63 0.4486286917551965 1106 64-65 0.6935852463252274 1106 66-67 0.9251950972747522 1106 68-69 0.9956868073446685 1106 70-71 0.6084423213007 1106 72-73 0.8574799736487293 1106 74-75 1.5212621376062287 1106 76 1.7214008324622654 1107 1 0.071640163192491 1107 2 -0.00907511896843971 1107 3 -0.019064183656951172 1107 4 0.2607362963451081 1107 5 0.08899330557000695 1107 6 0.06659444434840367 1107 7 0.39404906428296016 1107 8 0.3497005301187599 1107 9 0.17322895336695154 1107 10-11 -0.06178063358681385 1107 12-13 0.0805575390284119 1107 14-15 0.11756345117052547 1107 16-17 0.22174053784525682 1107 18-19 -0.31526060539327716 1107 20-21 -0.08388888902725 1107 22-23 -0.08618091001493866 1107 24-25 0.21298622542288115 1107 26-27 0.006141773046401511 1107 28-29 -0.01749807651231805 1107 30-31 -0.08104463441756593 1107 32-33 0.24938644984439406 1107 34-35 -0.006461766832941862 1107 36-37 0.1560479617499766 1107 38-39 -0.11933355133446355 1107 40-41 0.11615548682711818 1107 42-43 -8.982177724092821E-4 1107 44-45 0.09013844076617517 1107 46-47 -0.1045399567668639 1107 48-49 -0.0701290361079927 1107 50-51 0.16159528281050228 1107 52-53 -0.04265554516673831 1107 54-55 0.1386475435333736 1107 56-57 -0.04352874685621089 1107 58-59 0.140716957176231 1107 60-61 -0.26971970856016725 1107 62-63 0.15073302969015145 1107 64-65 0.4728417480572986 1107 66-67 0.5025333941570267 1107 68-69 0.48854653538354853 1107 70-71 0.16135065075626187 1107 72-73 0.4405780554438792 1107 74-75 0.1771899593320967 1107 76 -0.09443082050293583 1108 1 -0.15867922993869854 1108 2 0.0862072193128327 1108 3 -0.07775075865203007 1108 4 0.05960169651562808 1108 5 -0.10077343469626499 1108 6 -0.17129987828019466 1108 7 -0.07008752492194503 1108 8 -0.0373313654718217 1108 9 0.029589489405253744 1108 10-11 -0.11453733953089085 1108 12-13 -0.29085448029695016 1108 14-15 0.1098081662088859 1108 16-17 -0.023296954230026756 1108 18-19 0.1985447855955087 1108 20-21 -0.015086012084331912 1108 22-23 -0.17129229317738748 1108 24-25 0.1322361653043984 1108 26-27 -0.04328655478517618 1108 28-29 -0.06070687348671555 1108 30-31 0.19815108951833338 1108 32-33 -0.2925926507830212 1108 34-35 0.28611305335812887 1108 36-37 -0.016257090389146356 1108 38-39 -0.22446985634288552 1108 40-41 -0.5088745724169144 1108 42-43 0.05496278828317003 1108 44-45 0.035239330519793555 1108 46-47 -0.14248565337618402 1108 48-49 0.20518631268853937 1108 50-51 0.34783689354875946 1108 52-53 0.09144694045947688 1108 54-55 0.1417627742790657 1108 56-57 -0.023328934863116046 1108 58-59 0.18006724027965504 1108 60-61 0.3391548733430696 1108 62-63 0.17903244162203436 1108 64-65 0.662100213833483 1108 66-67 0.3138815785786875 1108 68-69 0.18454373714119043 1108 70-71 0.5273301448865055 1108 72-73 0.08469846731017583 1108 74-75 -0.2955217123990934 1108 76 0.10192709218315699 1109 1 0.0362632436950463 1109 2 0.11300491825817716 1109 3 -0.017156319534102238 1109 4 0.14354225260383657 1109 5 0.2866666420055566 1109 6 0.032031857098516525 1109 7 0.20141295446444474 1109 8 0.3675344063402548 1109 9 0.2646086648606669 1109 10-11 0.1269175981488786 1109 12-13 0.11076344875386468 1109 14-15 0.23208764847159102 1109 16-17 0.3607994024334431 1109 18-19 0.2966751786923467 1109 20-21 0.30721743949764146 1109 22-23 0.1474277451735233 1109 24-25 0.3283771049017119 1109 26-27 0.2286813263269991 1109 28-29 0.07132812171941794 1109 30-31 0.19610171272063326 1109 32-33 0.4296388928411403 1109 34-35 0.2616164090628317 1109 36-37 0.07554540242004748 1109 38-39 -0.05680446803990691 1109 40-41 0.48838094052651826 1109 42-43 0.46317228013359824 1109 44-45 0.4666391387652382 1109 46-47 0.35823342620195575 1109 48-49 0.1981992561209509 1109 50-51 0.02230956852478272 1109 52-53 0.3730145339398234 1109 54-55 0.018236887414246894 1109 56-57 0.33346636715030087 1109 58-59 0.0859996467993085 1109 60-61 0.5279012779451762 1109 62-63 0.22680329492980178 1109 64-65 0.16997413521411886 1109 66-67 0.46626652584618 1109 68-69 0.26076617242403444 1109 70-71 0.2441566070149861 1109 72-73 -0.6403374866687273 1109 74-75 -0.16961087826678067 1109 76 -0.22105948499805095 1110 1 0.12756360999541272 1110 2 -0.029669074415814123 1110 3 0.04621364383586268 1110 4 0.03163749069906885 1110 5 0.057362612701531646 1110 6 -0.06989121982455515 1110 7 -0.062048583997096785 1110 8 0.04876151256736705 1110 9 0.3639676392196449 1110 10-11 -0.015802182070899562 1110 12-13 0.170241470731888 1110 14-15 0.08130926019320128 1110 16-17 0.22897705811109859 1110 18-19 0.311876643893811 1110 20-21 0.3327211025013028 1110 22-23 0.008508331254112989 1110 24-25 0.13396318548779362 1110 26-27 0.18550367064934647 1110 28-29 0.3704581583494573 1110 30-31 0.10365665777558775 1110 32-33 0.3585308342330791 1110 34-35 -0.0321564847100646 1110 36-37 -0.059985733111084016 1110 38-39 -0.3750279112633521 1110 40-41 0.053353467999052384 1110 42-43 0.22823638269769475 1110 44-45 0.13623620836231254 1110 46-47 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-0.07206351672459732 2116 6 -0.051830576763912006 2116 7 -0.3258357227842339 2116 8 -0.26579892699307806 2116 9 -0.4040421287901239 2116 10-11 -0.1644845182532393 2116 12-13 -0.0035222655318492 2116 14-15 -0.28074303935909484 2116 16-17 -0.6809995393654944 2116 18-19 -0.5084835514663837 2116 20-21 -0.26712118484098113 2116 22-23 -0.20323363048785126 2116 24-25 -0.4605117839871724 2116 26-27 -0.28547211282644014 2116 28-29 -0.27662161373032035 2116 30-31 -0.4921919380730202 2116 32-33 -0.20091666271441966 2116 34-35 -0.22403173908532636 2116 36-37 -0.7243884600138131 2116 38-39 -0.017783304018742285 2116 40-41 -0.014793662648081352 2116 42-43 0.005963285424606113 2116 44-45 -0.420794723668628 2116 46-47 -0.3527454097768796 2116 48-49 -0.5125150295933381 2116 50-51 -0.6184973097820929 2116 52-53 -0.6171971062718171 2116 54-55 -0.7214456522682937 2116 56-57 -0.7702373365534001 2116 58-59 -0.25480723150757 2116 60-61 -0.3584743834304831 2116 62-63 -0.2608289802024828 2116 64-65 -0.7080681928282075 2116 66-67 -0.9269213048416489 2116 68-69 -1.1492867376288771 2116 70-71 -0.5978407474823655 2116 72-73 -0.8082607670920154 2116 74-75 -0.6453383914942954 2116 76 -0.9155039294424938 2117 1 -0.0218869394551362 2117 2 -0.0853467300934696 2117 3 0.0711220687442875 2117 4 -0.6247727657111852 2117 5 -0.22761907228015588 2117 6 -0.10945165938499457 2117 7 -0.18659070853922088 2117 8 -1.1138758500700021 2117 9 -0.345922470670466 2117 10-11 -0.04400731027602944 2117 12-13 -0.36602226553184636 2117 14-15 -0.03077865189471396 2117 16-17 -0.336590707456665 2117 18-19 -0.25442372240655686 2117 20-21 -0.5218576520774505 2117 22-23 -0.4142022914565118 2117 24-25 -0.6722639207393115 2117 26-27 -0.4939835088378359 2117 28-29 -1.4603110724197776 2117 30-31 -0.567405613286688 2117 32-33 -0.7161944404922025 2117 34-35 -0.599922052475641 2117 36-37 -0.32848390160924623 2117 38-39 -0.3757605119959564 2117 40-41 -0.539686825041251 2117 42-43 -0.33513358067226307 2117 44-45 -0.4271337550076524 2117 46-47 -0.5985431305746047 2117 48-49 -0.38527143984975254 2117 50-51 -0.23584061462539552 2117 52-53 -0.06755323162794014 2117 54-55 -3.9864372128306513E-4 2117 56-57 -0.4354438892599575 2117 58-59 -0.6801633568636873 2117 60-61 -0.33450714696324724 2117 62-63 -0.19398425085774562 2117 64-65 -0.3219214691814827 2117 66-67 -0.2766364045567542 2117 68-69 0.106553718211579 2117 70-71 0.21173902459740646 2117 72-73 0.435257751426505 2117 74-75 0.5061217224658208 2117 76 -0.7909312798698451 2118 1 -0.40015725670850344 2118 2 -0.054751336786210913 2118 3 -0.36025559314185074 2118 4 -0.1774020573235262 2118 5 -0.36208234911631365 2118 6 -0.12614005573441034 2118 7 -0.41866372027325127 2118 8 -0.7693300004394032 2118 9 0.03851907648294173 2118 10-11 -0.31023938328776524 2118 12-13 -0.4812765028199877 2118 14-15 -0.7781928196478631 2118 16-17 -0.3455867961137713 2118 18-19 -0.9023159431801346 2118 20-21 -0.7019532626815348 2118 22-23 -0.4883874283535121 2118 24-25 -0.3110296747592969 2118 26-27 -0.479641918225056 2118 28-29 -0.2638312810247285 2118 30-31 -0.47335954635926925 2118 32-33 -0.4811661919046202 2118 34-35 -0.5423383931970633 2118 36-37 -0.3703787299447612 2118 38-39 -0.25585612260003643 2118 40-41 -0.09235957601908495 2118 42-43 -0.42678938249755305 2118 44-45 -0.577025106593922 2118 46-47 -0.21440579897964795 2118 48-49 -0.6625856510622654 2118 50-51 -0.7056363556075738 2118 52-53 -0.30497174531764415 2118 54-55 -0.4927497953944666 2118 56-57 -0.33666075149376695 2118 58-59 -0.9588248084065043 2118 60-61 -1.2174276163243931 2118 62-63 -0.913410587233237 2118 64-65 -1.2880666234622353 2118 66-67 -0.7114473563168531 2118 68-69 -1.0491611883507872 2118 70-71 -0.6779176464151959 2118 72-73 -0.6025953559181261 2118 74-75 0.46479186588172183 2118 76 0.2062004020075996 2119 1 0.11579286041978776 2119 2 -0.4376919458533166 2119 3 -0.11924691186859349 2119 4 -0.25088283450418203 2119 5 -0.5056678527679566 2119 6 -0.44331657495722965 2119 7 -0.8659215403090741 2119 8 -0.2038070570743784 2119 9 -0.29767356510448906 2119 10-11 -0.25431062484763345 2119 12-13 -0.7519978752879481 2119 14-15 -1.2059349996120332 2119 16-17 -0.5499584372878061 2119 18-19 -0.2622098387292553 2119 20-21 -0.17131043506681465 2119 22-23 -0.14508096562786932 2119 24-25 -0.23930582192755878 2119 26-27 -0.13094410921306832 2119 28-29 -0.45537048455235407 2119 30-31 -0.3278965451190885 2119 32-33 -0.5462757413052088 2119 34-35 -0.5377738348396122 2119 36-37 -0.44361836064614835 2119 38-39 -0.8390344331967015 2119 40-41 -0.32559989571041825 2119 42-43 -0.7458433993089173 2119 44-45 -0.4616709657643767 2119 46-47 -0.31424044139387064 2119 48-49 -0.739649175934801 2119 50-51 -0.11889252206754719 2119 52-53 -0.6059053266873633 2119 54-55 -0.49905405335230313 2119 56-57 -0.43696046211799455 2119 58-59 -0.3359951267198511 2119 60-61 -0.6707485478387909 2119 62-63 -0.6836496667426744 2119 64-65 -0.9847732515454624 2119 66-67 -0.9176620455823894 2119 68-69 -0.6129272073669085 2119 70-71 -0.8502684800930211 2119 72-73 -0.21860403719138333 2119 74-75 -0.17588265527026437 2119 76 -0.39483997280292726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 5.0 22 1.0 23 22.0 24 24.0 25 95.0 26 146.0 27 301.0 28 560.0 29 932.0 30 1370.0 31 2173.0 32 3369.0 33 5437.0 34 8863.0 35 12526.0 36 16800.0 37 48181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.79864293792036 16.144872329028033 13.155962938713966 37.900521794337635 2 17.60411086641668 19.52066345257227 38.35386782532786 24.521357855683192 3 17.211277106521436 17.552526635319328 35.34115032835347 29.895045929805764 4 13.751165605221912 27.3455945082634 32.09134376922009 26.811896117294605 5 14.180703529551813 29.52602027657084 41.636410531119175 14.65686566275817 6 26.286133761879256 38.90641430073607 16.625002480011112 18.18244945737357 7 24.87847945558796 24.44298950459298 22.995655020534493 27.68287601928457 8 21.321151518758803 38.46794833640855 15.51494950697379 24.695950637858857 9 20.483899767870962 15.859175049104222 16.20439259567883 47.45253258734599 10-11 24.8665754022578 28.038509612523065 23.92317917584271 23.171735809376425 12-13 24.238636589091918 17.879392099676604 26.400710275182032 31.48126103604944 14-15 20.764141023351783 17.231117195405034 20.005753625776244 41.99898815546694 16-17 27.800924548141975 25.8739559153225 18.003888657421186 28.32123087911434 18-19 30.814138047338453 23.616649802591116 18.587683272821064 26.98152887724937 20-21 24.306588893518246 20.904013649981152 18.344146181774896 36.445251274725706 22-23 21.842449854175346 25.364065631014025 19.931849294684838 32.861635220125784 24-25 27.707676130389064 27.59756363708509 18.228577664027934 26.46618256849791 26-27 28.660496399023867 27.84258873479753 22.34589210959665 21.15102275658195 28-29 24.33783703350991 23.726266293672996 24.724718766740075 27.211177906077015 30-31 21.11927861436819 28.43233537686249 22.539332976211735 27.909053032557583 32-33 20.526059956748608 21.328095549868063 32.40878519135766 25.73705930202567 34-35 27.30789833938456 24.050651746919826 26.20826141301113 22.433188500684484 36-37 24.494573735690338 33.33779735333214 21.015118147729304 21.15251076324822 38-39 20.26863480348392 25.98208439973811 27.52762732377041 26.22165347300756 40-41 22.063666845227466 18.497410868400692 28.489375632402837 30.949546653969012 42-43 28.44919945241355 17.899728190782294 27.383786679364324 26.267285677439833 44-45 21.165406821022557 24.88244747336468 30.646489296272044 23.305656409340713 46-47 26.188421324127532 19.982937523560103 27.66105192151261 26.167589230799752 48-49 26.20826141301113 18.06340892407198 22.762037973930124 32.966291688986765 50-51 23.45594508263397 18.63678749280797 27.834156697022 30.07311072753606 52-53 18.549491101720136 18.60504335059421 38.228379263139104 24.617086284546556 54-55 20.104954070194232 20.37527528123326 30.956986687300358 28.56278396127215 56-57 24.746542864512033 22.433188500684484 24.09082792690911 28.729440707894373 58-59 20.04791381465389 23.01847112275063 31.95196714481281 24.98164791778267 60-61 24.719262742297087 28.133742039164332 24.749022875622483 22.3979723429161 62-63 19.39120687260679 38.15745094538023 19.793464674721744 22.657877507291232 64-65 18.15864135071325 39.994643176001425 19.121381663789855 22.725333809495467 66-67 18.107553121837984 39.364720353947185 19.178421919330198 23.34930460488463 68-69 17.84963196635121 39.405392536158566 19.52016745035018 23.22480804714005 70-71 17.892288157450945 37.544392198877055 19.92589726801976 24.637422375652243 72-73 18.29057794178918 33.46526992440926 21.110846576592664 27.1333055572089 74-75 18.281153899569468 31.992143324802097 22.45402059401226 27.272682181616176 76 18.479058786183362 32.64289824018412 21.738785389758547 27.139257583873977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.5 15 2.0 16 1.5 17 0.0 18 3.5 19 7.0 20 8.0 21 9.0 22 12.0 23 40.0 24 60.5 25 56.0 26 103.5 27 193.0 28 264.5 29 294.0 30 371.0 31 533.0 32 768.5 33 919.0 34 1047.0 35 1336.5 36 1772.5 37 2047.0 38 4240.5 39 7825.0 40 8834.5 41 7586.0 42 6719.0 43 6078.0 44 5212.5 45 4611.0 46 4234.0 47 4331.0 48 4150.5 49 3730.0 50 3587.0 51 3215.0 52 2494.0 53 2000.0 54 1855.0 55 1854.5 56 1713.5 57 1500.0 58 1427.0 59 1411.0 60 1406.5 61 1449.0 62 1480.0 63 1569.5 64 1703.0 65 1801.0 66 1934.5 67 2014.0 68 2125.0 69 2188.0 70 2099.5 71 2059.0 72 1932.5 73 1682.0 74 1360.0 75 1162.0 76 972.0 77 639.0 78 377.0 79 258.0 80 173.0 81 59.0 82 17.5 83 5.0 84 2.5 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 100806.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.556058171140606 #Duplication Level Percentage of deduplicated Percentage of total 1 62.05628894034518 25.78814753090094 2 19.603256069322768 16.29268099121084 3 7.657969492253707 9.547050770787454 4 3.8122747129455012 6.336924389421264 5 2.103077033252966 4.369779576612503 6 1.348738392494808 3.362895065769895 7 0.8283402162755723 2.409578794913001 8 0.630206965696689 2.0951133861079696 9 0.4702680766751809 1.7588238795309803 >10 1.4322885584015659 8.415173699978176 >50 0.02387147597335943 0.7092831775886356 >100 0.02148432837602349 1.7667599150844198 >500 0.0023871475973359433 0.6785310398190584 >1k 0.0071614427920078305 7.583873975755412 >5k 0.0023871475973359433 8.885383806519453 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 8957 8.885383806519453 No Hit CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG 3477 3.4491994524135468 TruSeq Adapter, Index 12 (96% over 26bp) TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 2157 2.1397535860960657 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 2011 1.9949209372457988 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 684 0.6785310398190584 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 481 0.47715413765053666 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT 249 0.24700910660079758 No Hit CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 184 0.18252881772910343 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 179 0.17756879550820387 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT 176 0.17459278217566415 No Hit CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 140 0.1388806221851874 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT 138 0.13689661329682756 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT 118 0.11705652441322938 RNA PCR Primer, Index 12 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC 116 0.11507251552486954 RNA PCR Primer, Index 12 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.9200444417991E-4 0.0 3 0.0 0.0 0.0 0.0029760133325397297 0.0 4 0.0 0.0 0.0 0.01289605777433883 0.0 5 0.0 0.0 0.0 0.01587207110687856 0.0 6 0.0 0.0 0.0 0.01686407555105847 0.0 7 0.0 0.0 0.0 0.01686407555105847 0.0 8 0.0 0.0 0.0 0.01686407555105847 0.0 9 0.0 0.0 0.0 0.02083209332777811 0.0 10 0.0 0.0 0.0 0.02281610221613793 0.0 11 0.0 0.0 0.0 0.02281610221613793 0.0 12 0.0 0.0 0.0 0.02281610221613793 0.0 13 0.0 0.0 0.0 0.024800111104497747 0.0 14 0.0 0.0 0.0 0.02678411999285757 0.0 15 0.0 0.0 0.0 0.02678411999285757 0.0 16 0.0 0.0 0.0 0.02678411999285757 0.0 17 0.0 0.0 0.0 0.02678411999285757 0.0 18 0.0 0.0 0.0 0.028768128881217388 0.0 19 0.0 0.0 0.0 0.044640199988095944 0.0 20 0.0 0.0 0.0 0.04860821776481559 0.0 21 0.0 0.0 0.0 0.052576235541535225 0.0 22 0.0 0.0 0.0 0.05852826220661469 0.0 23 0.0 0.0 0.0 0.06051227109497451 0.0 24 0.0 0.0 0.0 0.062496279983334325 0.0 25 0.0 0.0 0.0 0.062496279983334325 0.0 26 0.0 0.0 0.0 0.07737634664603298 0.0 27 0.0 0.0 0.0 0.1398726266293673 0.0 28 0.0 0.0 0.0 0.2103049421661409 0.0 29 0.0 0.0 0.0 0.2628811777076761 0.0 30 0.0 0.0 0.0 0.31744142213757115 0.0 31 0.0 0.0 0.0 0.40275380433704344 0.0 32 0.0 0.0 0.0 0.5029462531992144 0.0 33 0.0 0.0 0.0 0.6338908398309624 0.0 34 0.0 0.0 0.0 0.8223716842251453 0.0 35 0.0 0.0 0.0 1.0584687419399639 0.0 36 0.0 0.0 0.0 1.3927742396285936 0.0 37 0.0 0.0 0.0 1.8639763506140508 0.0 38 0.0 0.0 0.0 2.3421224927087674 0.0 39 0.0 0.0 0.0 2.841100728131262 0.0 40 0.0 0.0 0.0 3.346030990218836 0.0 41 0.0 0.0 0.0 3.899569470071226 0.0 42 0.0 0.0 0.0 4.4957641410233515 0.0 43 0.0 0.0 0.0 5.127670971965954 0.0 44 0.0 0.0 0.0 5.754617780687657 0.0 45 0.0 0.0 0.0 6.363708509414122 0.0 46 0.0 0.0 0.0 6.992639327024185 0.0 47 0.0 0.0 0.0 7.583873975755411 0.0 48 0.0 0.0 0.0 8.108644326726584 0.0 49 0.0 0.0 0.0 8.605638553260718 0.0 50 0.0 0.0 0.0 9.196873201991945 0.0 51 0.0 0.0 0.0 9.766283752951214 0.0 52 0.0 0.0 0.0 10.297998135031644 0.0 53 0.0 0.0 0.0 10.90014483264885 0.0 54 0.0 0.0 0.0 11.410035116957324 0.0 55 0.0 0.0 0.0 11.970517627918973 0.0 56 0.0 0.0 0.0 12.509176041108665 0.0 57 0.0 0.0 0.0 13.041882427633276 0.0 58 0.0 0.0 0.0 13.515068547507093 0.0 59 0.0 0.0 0.0 13.995198698490169 0.0 60 0.0 0.0 0.0 14.506080987242823 0.0 61 0.0 0.0 0.0 15.008035235997857 0.0 62 0.0 0.0 0.0 15.452453226990457 0.0 63 0.0 0.0 0.0 15.96730353351983 0.0 64 0.0 0.0 0.0 16.476201813384122 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCAGTA 15 0.0022190048 70.0 28 TCTTTAT 100 0.0 59.499996 1 CTTTATA 100 0.0 59.499996 2 AAATAAC 25 2.3805512E-4 55.999996 69 GTACCCT 20 0.0069337445 52.5 47 ATGATTG 20 0.0069337445 52.5 5 GACACAT 20 0.0069337445 52.5 26 CACATGT 20 0.0069337445 52.5 28 GCTATGG 20 0.0069337445 52.5 1 ATACTGG 20 0.0069337445 52.5 6 TAACACA 20 0.0069337445 52.5 3 TAGGGCA 20 0.0069337445 52.5 5 CTTCTCT 20 0.0069337445 52.5 32 GCTGTAA 20 0.0069337445 52.5 29 GATTCAG 20 0.0069337445 52.5 11 TAAGAAT 20 0.0069337445 52.5 12 ACCTTAG 20 0.0069337445 52.5 17 AATAACA 20 0.0069337445 52.5 70 TTTATAC 140 0.0 50.0 3 AAACTCG 45 1.6704198E-6 46.666668 36 >>END_MODULE