FastQCFastQC Report
Wed 25 May 2016
SRR1780333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404145
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAACG451.0436124E-634.1857451
AAAACGG551.3827594E-731.965892
CATCGTT353.2272274E-431.40673332
ATACTTT300.005753110629.3202127
TACACAG300.00577403329.298445
ATACTGG450.001406322524.4153676
CAGTGTA551.5812035E-424.02198234
GCCTAAG500.002589776621.9819933
TCTACGT500.002593502421.9765512
GCATGTA703.235046E-521.9765512
GTATTTC550.004458056320.01583739
GATCCTA550.0045062619.9786821
GTATATG550.0045062619.9786821
AGCATAT550.004509488619.976215
AAACGGG901.0085001E-519.5347123
GCAGTGT708.155319E-418.84170733
TCTTTAT708.1689056E-418.8370441
TCGTTGT600.00736476718.35012434
TTTCAAC600.007444143318.31605742
CTACGTC600.007449459818.3137913