Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780333_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 404145 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 58 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT | 25854 | 6.397208922540178 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC | 7620 | 1.8854619010503657 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG | 6243 | 1.5447426047581931 | No Hit |
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG | 4408 | 1.090697645646983 | Illumina PCR Primer Index 2 (95% over 24bp) |
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC | 1383 | 0.3422039119622908 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT | 710 | 0.17567952096401043 | Illumina PCR Primer Index 2 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCTTCT | 535 | 0.0 | 36.603996 | 43 |
TCAAGAC | 25 | 0.002350524 | 35.201286 | 3 |
GTATCGG | 25 | 0.0023519516 | 35.196934 | 14 |
TCTTCTG | 565 | 0.0 | 34.660423 | 44 |
CGTCTTC | 570 | 0.0 | 34.352135 | 42 |
ATGCCGT | 3375 | 0.0 | 33.901733 | 44 |
TCTTTAT | 155 | 0.0 | 32.707066 | 1 |
CTTTATA | 155 | 0.0 | 32.646355 | 2 |
TGCAAGG | 35 | 3.2157454E-4 | 31.42583 | 31 |
CCGTCTT | 665 | 0.0 | 29.444685 | 41 |
TCCTATA | 45 | 4.0619074E-5 | 29.334406 | 2 |
CTACTGC | 30 | 0.005742993 | 29.330774 | 8 |
TATGCCG | 3995 | 0.0 | 28.860695 | 43 |
GCCGTCT | 710 | 0.0 | 27.888344 | 40 |
TCTGCAA | 40 | 7.0275593E-4 | 27.4976 | 29 |
GGCTATA | 40 | 7.0275593E-4 | 27.4976 | 30 |
TTTATAC | 225 | 0.0 | 26.400967 | 3 |
TATGGGT | 45 | 0.0013963078 | 24.44534 | 4 |
CAAGGTT | 45 | 0.0013973151 | 24.442314 | 33 |
TTAATAC | 75 | 2.0579573E-6 | 23.467525 | 3 |