FastQCFastQC Report
Wed 25 May 2016
SRR1780333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404145
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT258546.397208922540178No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC76201.8854619010503657No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG62431.5447426047581931No Hit
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG44081.090697645646983Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC13830.3422039119622908No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT7100.17567952096401043Illumina PCR Primer Index 2 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCT5350.036.60399643
TCAAGAC250.00235052435.2012863
GTATCGG250.002351951635.19693414
TCTTCTG5650.034.66042344
CGTCTTC5700.034.35213542
ATGCCGT33750.033.90173344
TCTTTAT1550.032.7070661
CTTTATA1550.032.6463552
TGCAAGG353.2157454E-431.4258331
CCGTCTT6650.029.44468541
TCCTATA454.0619074E-529.3344062
CTACTGC300.00574299329.3307748
TATGCCG39950.028.86069543
GCCGTCT7100.027.88834440
TCTGCAA407.0275593E-427.497629
GGCTATA407.0275593E-427.497630
TTTATAC2250.026.4009673
TATGGGT450.001396307824.445344
CAAGGTT450.001397315124.44231433
TTAATAC752.0579573E-623.4675253