FastQCFastQC Report
Wed 25 May 2016
SRR1780332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215999
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACCG200.006944432452.539
GAGAACA200.006944432452.545
GAATTGA200.006944432452.527
AGAGATC200.006944432452.57
GACAAAT305.8687443E-446.66666447
AGTCTTG305.8687443E-446.66666411
GGACAAA305.8687443E-446.66666446
TGTAAAC305.8687443E-446.66666416
TAAACCG350.00125350640.018
CGAATAT350.00125350640.069
CCCATAG459.1770984E-538.8888851
TTAATAC400.002415099635.03
AAAAGCA400.002415099635.015
AAAGTTG400.002415099635.015
TAGGGAT400.002415099635.069
GAGATCA400.002415099635.08
GTAAACC552.9854683E-431.8181817
TCATTAG450.004300792731.11110935
ATTGTTA450.004300792731.11110939
GTATAAA604.971058E-429.1666661