FastQCFastQC Report
Wed 25 May 2016
SRR1780332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215999
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT100654.659743795110162TruSeq Adapter, Index 5 (95% over 23bp)
CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG31821.4731549683100384TruSeq Adapter, Index 5 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC29331.3578766568363743RNA PCR Primer, Index 5 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG24271.1236163130384864TruSeq Adapter, Index 5 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6000.277779063791962RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT3800.17592674040157594TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT2970.1375006365770212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA150.002222451870.03
AATGCGT252.386677E-455.9999966
AAATGCG252.386677E-455.9999965
TCTTTAT900.054.4444431
TTGCTCC200.006944432452.567
ATCGTAT200.006944432452.539
CTTTATA1000.048.9999962
ATAAATG305.8687443E-446.6666643
AAAGCAG305.8687443E-446.6666647
ATAAGAC350.00125350640.03
TTAAATG350.00125350640.024
TAAGACA350.00125350640.04
GCCTAAA459.1770984E-538.8888852
TTAATAC400.002415099635.03
CAGTTAT400.002415099635.034
TAAATGT400.002415099635.025
CTCTATA501.7058967E-435.01
TGTGTAC400.002415099635.066
AATGTCC501.7058967E-435.027
TCAGTTA400.002415099635.033
Produced by FastQC (version 0.11.5)