##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780332_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 215999 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.71294774512845 34.0 34.0 34.0 34.0 34.0 2 33.75055903036588 34.0 34.0 34.0 34.0 34.0 3 33.76548502539364 34.0 34.0 34.0 34.0 34.0 4 33.780096204149096 34.0 34.0 34.0 34.0 34.0 5 33.76402668530873 34.0 34.0 34.0 34.0 34.0 6 37.52248853003949 38.0 38.0 38.0 37.0 38.0 7 37.525909842175196 38.0 38.0 38.0 37.0 38.0 8 37.41211764869282 38.0 38.0 38.0 37.0 38.0 9 37.45159468330872 38.0 38.0 38.0 37.0 38.0 10-11 37.41058986384196 38.0 38.0 38.0 37.0 38.0 12-13 37.386712438483514 38.0 38.0 38.0 37.0 38.0 14-15 37.31372367464664 38.0 38.0 38.0 36.0 38.0 16-17 37.345765489655044 38.0 38.0 38.0 36.5 38.0 18-19 37.300774540622875 38.0 38.0 38.0 36.0 38.0 20-21 37.32504317149616 38.0 38.0 38.0 36.5 38.0 22-23 37.30790188843467 38.0 38.0 38.0 36.0 38.0 24-25 37.316996837948324 38.0 38.0 38.0 36.5 38.0 26-27 37.22089454117843 38.0 38.0 38.0 36.0 38.0 28-29 37.202507881980935 38.0 38.0 38.0 36.0 38.0 30-31 37.19417219524165 38.0 38.0 38.0 36.0 38.0 32-33 37.10203519460738 38.0 38.0 38.0 36.0 38.0 34-35 37.131315422756586 38.0 38.0 38.0 36.0 38.0 36-37 37.147567812813946 38.0 38.0 38.0 36.0 38.0 38-39 36.66404011129681 38.0 38.0 38.0 34.0 38.0 40-41 36.87184199926851 38.0 38.0 38.0 35.5 38.0 42-43 37.077030449215044 38.0 38.0 38.0 36.0 38.0 44-45 37.14532706169936 38.0 38.0 38.0 36.0 38.0 46-47 37.0930189491618 38.0 38.0 38.0 36.0 38.0 48-49 37.11540794170344 38.0 38.0 38.0 36.0 38.0 50-51 36.98649762267418 38.0 38.0 38.0 35.5 38.0 52-53 36.98846059472498 38.0 38.0 38.0 36.0 38.0 54-55 36.967536886744846 38.0 38.0 38.0 35.5 38.0 56-57 37.0121528340409 38.0 38.0 38.0 36.0 38.0 58-59 36.90783753628489 38.0 38.0 38.0 35.5 38.0 60-61 36.92625660303983 38.0 38.0 38.0 35.0 38.0 62-63 36.906115306089376 38.0 38.0 38.0 35.0 38.0 64-65 36.81658479900369 38.0 38.0 38.0 35.5 38.0 66-67 36.53613905619933 38.0 37.5 38.0 34.0 38.0 68-69 36.46351834962199 38.0 37.0 38.0 34.0 38.0 70-71 35.95722896865263 38.0 37.0 38.0 32.5 38.0 72-73 35.16600539817314 38.0 37.0 38.0 26.0 38.0 74-75 34.90779586942532 38.0 37.0 38.0 24.0 38.0 76 34.190963847054846 38.0 35.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.011132711451473654 1101 2 -0.18676994887997012 1101 3 0.003080531100984274 1101 4 0.0922724240845696 1101 5 -0.024446388107179473 1101 6 0.005234266690791856 1101 7 0.10069795969737783 1101 8 0.03554706190882939 1101 9 -0.006099541839674316 1101 10-11 0.09139759355985433 1101 12-13 0.16017878969348942 1101 14-15 0.21364693603329954 1101 16-17 0.11941337651832384 1101 18-19 0.11903203416352426 1101 20-21 -0.02208092864668032 1101 22-23 -0.10614872065896463 1101 24-25 -0.021308888834823847 1101 26-27 0.025991219367035967 1101 28-29 -0.07532438193678104 1101 30-31 0.04823675117012982 1101 32-33 0.08479550226063282 1101 34-35 0.16235483633948888 1101 36-37 0.24522502762752652 1101 38-39 0.23611962784859486 1101 40-41 0.034937010919676936 1101 42-43 0.202622671002473 1101 44-45 0.21125244097444806 1101 46-47 0.12000486417252887 1101 48-49 0.07231707390203468 1101 50-51 0.12698199471572025 1101 52-53 0.1480445167666531 1101 54-55 0.1423090393526394 1101 56-57 -0.05769594855747329 1101 58-59 -0.09288170258961514 1101 60-61 0.009679007500913883 1101 62-63 0.1509751455506887 1101 64-65 0.06269892824173695 1101 66-67 0.05026172829494868 1101 68-69 -0.41131748593081596 1101 70-71 -0.3123433179526671 1101 72-73 -0.011978831042767979 1101 74-75 -0.1493804428156622 1101 76 -1.0063519659534848 1102 1 -0.029552105142741425 1102 2 0.03064788075474212 1102 3 0.004110043798306151 1102 4 -0.009668398000385992 1102 5 -0.0022928000647723934 1102 6 -0.03390628085744396 1102 7 0.08958303557627545 1102 8 -0.034631386958700716 1102 9 -0.060320543898697565 1102 10-11 0.05473550250517434 1102 12-13 0.14464077398389463 1102 14-15 0.13060911097419137 1102 16-17 0.04444960011323218 1102 18-19 0.021390380842390755 1102 20-21 0.0662188501987444 1102 22-23 0.11717927446038345 1102 24-25 0.025810382117661845 1102 26-27 0.1800749530664234 1102 28-29 0.23862932812194515 1102 30-31 0.11983554625896886 1102 32-33 0.06432917113884429 1102 34-35 0.23098901616103973 1102 36-37 0.1222745204972 1102 38-39 0.19376661938371598 1102 40-41 0.15543765913137975 1102 42-43 0.10254069128768606 1102 44-45 0.1147546906503436 1102 46-47 0.022725446800457405 1102 48-49 0.06451946847231227 1102 50-51 -0.024909258239368626 1102 52-53 0.006677171384211533 1102 54-55 0.09940314443919362 1102 56-57 0.01418882228063012 1102 58-59 0.2981806019936215 1102 60-61 0.15618438384500166 1102 62-63 0.13897369660688952 1102 64-65 0.15089384931242478 1102 66-67 0.2518174363709065 1102 68-69 0.33734796057265015 1102 70-71 0.2503044361844502 1102 72-73 0.2684871187780189 1102 74-75 0.3482936210903773 1102 76 0.5115310509000253 1103 1 -0.09255135893798894 1103 2 -0.021531404111975405 1103 3 -0.023835935870671676 1103 4 0.0773195036963159 1103 5 0.08478073168562617 1103 6 0.06396275409184682 1103 7 0.018913775542266364 1103 8 -0.10338620364922235 1103 9 0.017682318591667467 1103 10-11 -0.058186072598495286 1103 12-13 0.0301289264543243 1103 14-15 0.05647183376524367 1103 16-17 0.007142420644875358 1103 18-19 0.10414265309702841 1103 20-21 0.012057841232056887 1103 22-23 0.010582090465860006 1103 24-25 -0.03508481892946946 1103 26-27 -0.006298858816585096 1103 28-29 0.09444360876833002 1103 30-31 0.005020935325234177 1103 32-33 0.137766116837156 1103 34-35 -0.10316086089866872 1103 36-37 0.01352435229530613 1103 38-39 -0.0525818704691261 1103 40-41 0.14293872950705122 1103 42-43 -0.003973920869341896 1103 44-45 0.013472686417770774 1103 46-47 0.041564058614959265 1103 48-49 0.10949665417943066 1103 50-51 -0.10378677056397834 1103 52-53 0.11517350764120238 1103 54-55 -0.05060541856617107 1103 56-57 -0.2199420938386183 1103 58-59 0.23513853317152922 1103 60-61 0.08462212467953378 1103 62-63 6.138791212393357E-4 1103 64-65 0.17513327094258102 1103 66-67 -0.2265579168530607 1103 68-69 0.009439902784599496 1103 70-71 -0.022259082966101573 1103 72-73 0.33159341014039967 1103 74-75 0.3826312726249981 1103 76 0.3987868523361584 1104 1 0.05224639728814395 1104 2 0.038916449547109266 1104 3 0.11030614727962273 1104 4 -0.055943993301845296 1104 5 0.03903230018718773 1104 6 -0.03456279812207441 1104 7 0.06507897226671844 1104 8 0.025839176226646998 1104 9 -0.02460245596087418 1104 10-11 0.12638460289908693 1104 12-13 0.01528025728705984 1104 14-15 -0.010204970433946414 1104 16-17 -0.07892241450758064 1104 18-19 0.011586981147580389 1104 20-21 0.07957099186452155 1104 22-23 -0.07862256378795962 1104 24-25 0.019866239316286283 1104 26-27 0.06006537146309654 1104 28-29 -0.00734284974100774 1104 30-31 0.0034101940463315827 1104 32-33 0.00796627678489159 1104 34-35 0.11600977630156706 1104 36-37 0.04925915444447071 1104 38-39 0.10069198934006351 1104 40-41 0.018970786637680703 1104 42-43 -0.06153539374017214 1104 44-45 -0.14283961716753169 1104 46-47 -0.20759677897051176 1104 48-49 0.07059668909759864 1104 50-51 -0.375844351748718 1104 52-53 -0.17013889731943266 1104 54-55 -0.22552854227786412 1104 56-57 -0.2974350820452898 1104 58-59 0.03089822859499236 1104 60-61 -0.050964556855518595 1104 62-63 -0.005167330399039827 1104 64-65 -0.23183797225682667 1104 66-67 -0.2043201128678902 1104 68-69 0.004050114659030157 1104 70-71 0.15762016775760657 1104 72-73 0.4723446013004704 1104 74-75 0.2542253432948627 1104 76 0.13910832475644241 1105 1 -0.013526875098719415 1105 2 0.1441952908895061 1105 3 0.0021683317944933833 1105 4 -0.06550483013510444 1105 5 -1.1905958252356186E-4 1105 6 0.13451586442901942 1105 7 0.04863916815001801 1105 8 0.010183692449992066 1105 9 0.13100220160880127 1105 10-11 0.030642703325817422 1105 12-13 0.020035375047555704 1105 14-15 0.049878465507603664 1105 16-17 0.06534895470770152 1105 18-19 0.14689489013268542 1105 20-21 -0.02172299613734907 1105 22-23 0.03306437151545083 1105 24-25 -0.01597916805239663 1105 26-27 0.22161568322340486 1105 28-29 0.29583350707696354 1105 30-31 0.07963966537506195 1105 32-33 0.19103198540004485 1105 34-35 0.2110910476314274 1105 36-37 0.05412531397353604 1105 38-39 0.06222936965390602 1105 40-41 0.05353288850373872 1105 42-43 -0.03986678735653726 1105 44-45 -0.057500504039865064 1105 46-47 0.11545746001387158 1105 48-49 0.057147984772313976 1105 50-51 -0.20070899323842184 1105 52-53 -0.012713998100380763 1105 54-55 -0.02317811132335379 1105 56-57 0.02150077235786796 1105 58-59 0.11739836072064236 1105 60-61 0.024088433963150635 1105 62-63 0.03400370765877625 1105 64-65 -0.0948517864150773 1105 66-67 -0.2458103777376479 1105 68-69 0.12960462070588363 1105 70-71 0.20184712026546237 1105 72-73 0.5138587612343528 1105 74-75 0.1674426871757504 1105 76 0.36204365065700017 1106 1 0.13537524743485818 1106 2 0.012204939574623097 1106 3 0.030963810966262884 1106 4 0.007199842745535534 1106 5 0.037164782412880015 1106 6 0.06789328804113381 1106 7 -0.0660762576410221 1106 8 0.10980311854808633 1106 9 0.10837739315055472 1106 10-11 0.06524127019493164 1106 12-13 0.12514492659627763 1106 14-15 0.10644196289023 1106 16-17 0.21077306283910957 1106 18-19 0.2785619455417674 1106 20-21 0.25376349777022966 1106 22-23 0.11929285167039438 1106 24-25 0.11927366706079567 1106 26-27 0.04730161675140465 1106 28-29 0.2548546438385131 1106 30-31 -0.030006362514008345 1106 32-33 0.12493494938872374 1106 34-35 -0.06103405459798239 1106 36-37 0.2119734328047116 1106 38-39 -0.0032712666042442606 1106 40-41 0.17990763422191947 1106 42-43 0.11755469897794057 1106 44-45 0.22396032105852726 1106 46-47 0.15702534182042882 1106 48-49 0.21531644703526354 1106 50-51 0.15798117118485777 1106 52-53 0.26704472572224347 1106 54-55 0.28059308374955805 1106 56-57 0.19419540682682168 1106 58-59 0.2655047915043198 1106 60-61 0.3572382218278918 1106 62-63 0.4349319654924244 1106 64-65 0.26062903874236554 1106 66-67 0.38732553349917254 1106 68-69 0.3112939672938637 1106 70-71 0.34437337514074073 1106 72-73 0.2875590083564319 1106 74-75 0.4551465186012962 1106 76 0.30539748400165223 1107 1 -0.07430686206463832 1107 2 -0.06882049864435658 1107 3 -0.019397930510706374 1107 4 0.09849594733650946 1107 5 -0.036631642045961144 1107 6 -0.07505275315784843 1107 7 -0.08932774357265316 1107 8 -0.04859544844387642 1107 9 0.08511136777049444 1107 10-11 -0.13884896743781638 1107 12-13 -0.06340991038742061 1107 14-15 -0.09403667575212893 1107 16-17 -0.04038718514572537 1107 18-19 -0.059522394890692 1107 20-21 -0.08994470131553811 1107 22-23 -3.726247000912508E-4 1107 24-25 0.06332150766191091 1107 26-27 -0.06442241288580419 1107 28-29 0.08715955351945581 1107 30-31 0.10319534034618982 1107 32-33 -0.030571721310217015 1107 34-35 -0.08695498326163431 1107 36-37 0.047631139293955016 1107 38-39 -0.07533022697552383 1107 40-41 0.008186570632190637 1107 42-43 0.028423518258406943 1107 44-45 -0.006011588727105277 1107 46-47 0.023534303936443735 1107 48-49 -0.17621926766586427 1107 50-51 -0.08857443348172467 1107 52-53 0.10728817424677572 1107 54-55 0.02237829538577074 1107 56-57 -0.058415878111475195 1107 58-59 0.06712710316029558 1107 60-61 -0.11727848690623688 1107 62-63 -0.06063059484628042 1107 64-65 0.08736097783952346 1107 66-67 0.21721398685080828 1107 68-69 0.2483105306335105 1107 70-71 -0.07258892697736741 1107 72-73 0.1720256432301639 1107 74-75 0.254851077009171 1107 76 0.2432791445229796 1108 1 -0.017679345286111925 1108 2 0.03434411731388565 1108 3 -0.004122483083413897 1108 4 -0.0018838997924959244 1108 5 -0.029174520494883893 1108 6 -0.08559758910117665 1108 7 -0.06873909704021486 1108 8 0.1315872510341265 1108 9 -0.08507412812809179 1108 10-11 -0.08322932724392729 1108 12-13 -0.08836213820248418 1108 14-15 0.08894244430751996 1108 16-17 0.06239874885874741 1108 18-19 0.14154607080654813 1108 20-21 0.1228104720625467 1108 22-23 -0.047527266758251585 1108 24-25 0.008925301472501701 1108 26-27 0.08682898532448746 1108 28-29 0.06552224926889494 1108 30-31 0.14909741901882256 1108 32-33 0.06715735214242358 1108 34-35 -0.0044780537314323965 1108 36-37 0.05462219074809127 1108 38-39 0.051685526193750775 1108 40-41 0.04799478278729197 1108 42-43 0.16823289558875842 1108 44-45 0.10307794333851916 1108 46-47 0.1001504019975954 1108 48-49 0.2645082677554669 1108 50-51 -0.03706203601714719 1108 52-53 -0.13968819599929816 1108 54-55 -0.10442190036366839 1108 56-57 0.10684675238815089 1108 58-59 0.07478230450258394 1108 60-61 0.23190122972314242 1108 62-63 -0.04886232489848652 1108 64-65 0.14008515755615747 1108 66-67 0.22137948834222954 1108 68-69 0.37251821146870867 1108 70-71 0.45516554729556447 1108 72-73 0.03923644673501059 1108 74-75 0.1485288361441448 1108 76 0.2767080222261882 1109 1 0.03371941137463352 1109 2 0.11439024914731277 1109 3 0.034630107206695016 1109 4 0.031620269341111396 1109 5 0.11716453614612732 1109 6 -0.2211838580966372 1109 7 0.171094014268256 1109 8 0.11809307301952288 1109 9 0.19009485746496324 1109 10-11 -0.011609347472862908 1109 12-13 0.06258547626366351 1109 14-15 0.16751457771979972 1109 16-17 0.2152304910138767 1109 18-19 0.10379105057761251 1109 20-21 0.1818182059287352 1109 22-23 0.05759225662836087 1109 24-25 0.021850540765967708 1109 26-27 0.07793873961696818 1109 28-29 0.058981766734859775 1109 30-31 0.15346236622624332 1109 32-33 0.24408495970114075 1109 34-35 0.12025829173926184 1109 36-37 0.08962811141120852 1109 38-39 -0.004742710435415631 1109 40-41 0.13062657921516063 1109 42-43 0.07789036055817178 1109 44-45 0.1435065130049864 1109 46-47 0.23848268688016105 1109 48-49 0.2485008915960023 1109 50-51 0.24798397246914305 1109 52-53 0.22016323236067592 1109 54-55 0.053664430305367716 1109 56-57 0.13313048783319203 1109 58-59 0.11100011089690298 1109 60-61 0.2920206297626109 1109 62-63 -0.11891733772659308 1109 64-65 0.16992591638781107 1109 66-67 0.2395654835070289 1109 68-69 0.10385578794394235 1109 70-71 0.1115635638734247 1109 72-73 -0.23193146573785128 1109 74-75 0.05538632084909523 1109 76 0.37404434056050917 1110 1 -0.06897244873438524 1110 2 0.04796959624108865 1110 3 0.04066918358324756 1110 4 -0.019185145003227433 1110 5 0.060672222800135955 1110 6 -0.3058442366107528 1110 7 0.010822684568985608 1110 8 -0.06716753823790356 1110 9 0.0623157186152028 1110 10-11 -0.12862563950446315 1110 12-13 0.0017659748243303852 1110 14-15 -0.06900774726335612 1110 16-17 0.024377915525420235 1110 18-19 0.06780924130463006 1110 20-21 -0.028500591155854238 1110 22-23 -0.153525829976644 1110 24-25 0.13338052177901716 1110 26-27 0.044192491071612494 1110 28-29 0.009451846970051747 1110 30-31 -0.06792245646010286 1110 32-33 0.13241717015009158 1110 34-35 0.0825370324754644 1110 36-37 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0.0942761977833797 2115 52-53 0.0936537068038632 2115 54-55 0.06019285435905175 2115 56-57 -2.922507603315694E-4 2115 58-59 -0.07486920372411277 2115 60-61 0.22959064674528662 2115 62-63 0.12146699665341743 2115 64-65 -0.1634994199372386 2115 66-67 -0.1959425840729594 2115 68-69 -0.11109188400153158 2115 70-71 -0.6382797604436234 2115 72-73 -0.6128319693730546 2115 74-75 -0.5153352964985487 2115 76 -0.8166388953037753 2116 1 -0.04095916179987569 2116 2 -0.01702565842515469 2116 3 -0.13180788063693427 2116 4 0.03393095749598274 2116 5 -0.07616738491893926 2116 6 0.13918898072553532 2116 7 -0.025858745358256385 2116 8 0.09425289833280459 2116 9 -0.012170203561325366 2116 10-11 0.02467236745535928 2116 12-13 0.006131681879061546 2116 14-15 0.14671512524534336 2116 16-17 -0.31461291016265847 2116 18-19 -0.2259822803657272 2116 20-21 0.13133015860688602 2116 22-23 0.15080033263012638 2116 24-25 0.17901481472428316 2116 26-27 0.1309134654468096 2116 28-29 0.015818503091530545 2116 30-31 0.10529804338171544 2116 32-33 0.03418876141866889 2116 34-35 0.19940669397499278 2116 36-37 -0.14637169304395314 2116 38-39 0.14803810009793494 2116 40-41 -6.752605358428809E-4 2116 42-43 -0.006114071791465392 2116 44-45 -0.04228552506107519 2116 46-47 0.13809159720655373 2116 48-49 0.0468827580714688 2116 50-51 0.07534558375859035 2116 52-53 -0.16671896817055654 2116 54-55 0.1015175109513109 2116 56-57 -0.0030713796199961507 2116 58-59 0.10606097422735417 2116 60-61 0.03666677508501692 2116 62-63 0.13805790955615294 2116 64-65 0.07280368456940778 2116 66-67 0.03738515857669 2116 68-69 0.03787244184587735 2116 70-71 0.19505469102644923 2116 72-73 0.08924441054745103 2116 74-75 0.34307840291275227 2116 76 0.11126141162475989 2117 1 0.029366109259342466 2117 2 0.04912947084923758 2117 3 -0.1375126345985649 2117 4 -0.22965536443896184 2117 5 -0.03321492453528663 2117 6 -0.05045238708097344 2117 7 -0.04318985461596725 2117 8 -0.2887662843194079 2117 9 -0.10172816853213362 2117 10-11 0.015214485887383944 2117 12-13 0.05703243250458456 2117 14-15 0.00980103016611622 2117 16-17 -0.17834935720185285 2117 18-19 -0.06393057060759588 2117 20-21 -0.06911187642230487 2117 22-23 0.10677197566599261 2117 24-25 -0.020976844992148358 2117 26-27 -0.09773540861490915 2117 28-29 -0.7724133567917022 2117 30-31 -0.21105725269834608 2117 32-33 -0.16516903674646244 2117 34-35 -0.08001782645870037 2117 36-37 -0.20916568803978208 2117 38-39 -0.018383918250691522 2117 40-41 -0.2728353939803867 2117 42-43 -0.21763200340113542 2117 44-45 -0.07233556676249009 2117 46-47 -0.20937295658826827 2117 48-49 -0.30644501507281774 2117 50-51 -0.19985875318886315 2117 52-53 -0.11479236266596615 2117 54-55 -0.078048794303065 2117 56-57 -0.3447978183189164 2117 58-59 0.005293668138946828 2117 60-61 -0.25047250765059204 2117 62-63 -0.05410222388838548 2117 64-65 -0.20609539799939824 2117 66-67 -0.34593427428402634 2117 68-69 -0.2711434046929 2117 70-71 -0.3091905133104973 2117 72-73 -0.42046367952761443 2117 74-75 -0.45579565880535 2117 76 -1.0421998060566438 2118 1 0.03816666231845289 2118 2 0.03760510168228137 2118 3 -0.0273916529186522 2118 4 0.022705506629129957 2118 5 0.026169797206847534 2118 6 0.1231252140251442 2118 7 0.07038083325129918 2118 8 0.08875877617143146 2118 9 -0.1080330423655198 2118 10-11 0.013963189655100905 2118 12-13 0.013959497834690637 2118 14-15 -0.002998831569115623 2118 16-17 0.054606052407542904 2118 18-19 -0.1539063742716138 2118 20-21 0.008751932772305793 2118 22-23 0.03847954566252554 2118 24-25 -0.10428828640245058 2118 26-27 -0.09023454446005275 2118 28-29 0.021340531905146065 2118 30-31 0.0419569194412972 2118 32-33 -0.049541660320606695 2118 34-35 -0.1810167894728778 2118 36-37 -0.07196209315557667 2118 38-39 0.06690125285197013 2118 40-41 -0.10540020559462704 2118 42-43 -0.07974054125112673 2118 44-45 -0.16965323008776068 2118 46-47 0.029157980155595453 2118 48-49 -0.24084529160236912 2118 50-51 -0.022796879701132866 2118 52-53 -0.17532468205760665 2118 54-55 0.0558779253651025 2118 56-57 -0.22206708897221716 2118 58-59 -0.38974953960387637 2118 60-61 -0.12641097982481142 2118 62-63 0.03960672875593474 2118 64-65 -0.130239884691413 2118 66-67 0.08441484427801527 2118 68-69 -0.06793911848481571 2118 70-71 -0.23712691151305165 2118 72-73 -0.046516220142898135 2118 74-75 0.23613313483447484 2118 76 -0.5592063736335504 2119 1 -0.009607389566554048 2119 2 -0.011179150418790584 2119 3 -0.045308676195347175 2119 4 0.06046875498405058 2119 5 -0.10748530360271502 2119 6 -0.07486701304541299 2119 7 -0.0876313172124128 2119 8 0.0012571977335937845 2119 9 -0.037347088357691405 2119 10-11 0.0658552104764496 2119 12-13 -0.008254383999940273 2119 14-15 -0.38671356225253817 2119 16-17 -0.027651604769786786 2119 18-19 0.03826667863762623 2119 20-21 0.07754025373535711 2119 22-23 0.10712756104001642 2119 24-25 -0.07560280943361164 2119 26-27 0.042452447809111504 2119 28-29 0.10828439564036074 2119 30-31 0.0460678730983588 2119 32-33 -0.23446792973539488 2119 34-35 -0.13544553528124936 2119 36-37 -0.007954685020663987 2119 38-39 -0.08181565256803935 2119 40-41 0.07868423030677008 2119 42-43 0.033002012029925254 2119 44-45 0.007191437598464745 2119 46-47 0.14485045837315624 2119 48-49 0.11422254614824823 2119 50-51 0.2085880767339816 2119 52-53 -0.02213553609770713 2119 54-55 0.08796932042353234 2119 56-57 -0.08430804978486606 2119 58-59 -0.15509546302509136 2119 60-61 0.17311689187976498 2119 62-63 0.0146498727234885 2119 64-65 -0.21661200316545148 2119 66-67 -0.05081518672951546 2119 68-69 -0.137450013213531 2119 70-71 0.1258681918220077 2119 72-73 -0.016283874093197426 2119 74-75 0.15180246263041397 2119 76 -1.1971478100821358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 5.0 21 4.0 22 9.0 23 19.0 24 37.0 25 70.0 26 161.0 27 333.0 28 582.0 29 928.0 30 1583.0 31 2524.0 32 4020.0 33 6454.0 34 10843.0 35 17736.0 36 28606.0 37 142083.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.996472205889845 17.48017351932185 11.91579590646253 32.607558368325776 2 20.291760610002825 18.79036477020727 34.693679137403414 26.22419548238649 3 19.24407057440081 18.887587442534457 30.547363645202065 31.320978337862677 4 15.736183963814648 21.12880152222927 31.475145718267218 31.659868795688865 5 16.552854411363015 30.215880629076985 36.24276038314992 16.988504576410076 6 30.668660503057882 35.407571331348755 16.076463316959803 17.84730484863356 7 28.695040254815996 24.8380779540646 21.376950819216756 25.089930971902646 8 25.024189926805217 34.41404821318617 16.711188477724434 23.850573382284175 9 23.24408909300506 15.480627225126042 17.89267542905291 43.382608252815984 10-11 27.618183417515823 25.015856554891457 23.039689998564807 24.326270029027913 12-13 26.308918096843044 19.043375200811116 24.315390349029396 30.33231635331645 14-15 23.569322080194816 18.880642965939657 20.38643697424525 37.16359797962028 16-17 27.75082292047648 24.320482965198913 19.918610734308956 28.01008338001565 18-19 29.272357742396952 23.02117139431201 20.277408691706906 27.42906217158413 20-21 25.564933170986905 21.813758397029616 20.435974240621483 32.185334191361996 22-23 24.79965185024005 24.79317033875157 20.248704855115072 30.158472955893313 24-25 27.892027277904063 24.979050828939023 20.171389682359642 26.957532210797275 26-27 27.926055213218582 26.733920064444742 20.54569697081931 24.79432775151737 28-29 25.88322168158186 25.024652891911536 21.877184616595446 27.21494080991116 30-31 25.022106583826776 25.396182389733283 20.743151588664762 28.838559437775174 32-33 23.628813096356925 28.5186505493081 21.72926726512623 26.123269089208744 34-35 27.695035625164934 23.133671915147755 24.62233621451951 24.5489562451678 36-37 29.216801929638564 22.689688378186936 23.40566391511072 24.68784577706378 38-39 24.290158750734957 23.29918194065713 25.74224880670744 26.668410501900468 40-41 24.108907911610704 20.32046444659466 26.308686614289883 29.261941027504758 42-43 27.04896781929546 19.863980851763202 26.0142408066704 27.072810522270935 44-45 23.007513923675575 23.698952309964398 27.979759165551695 25.313774600808337 46-47 25.433219598238882 21.3593581451766 26.420955652572463 26.786466604012055 48-49 25.21956120167223 20.523474645715954 24.003120384816594 30.253843767795217 50-51 23.727193181449916 21.06074565160024 26.17928786707346 29.03277329987639 52-53 21.00171760054445 20.975791554590533 31.58023879740184 26.44225204746318 54-55 21.390376807300036 21.72162834087195 28.325825582525844 28.562169269302174 56-57 23.90474029972361 22.58089157820175 24.772799874073492 28.741568248001148 58-59 21.981120282964273 22.697327302441213 28.35568683188348 26.96586558271103 60-61 24.067472534595066 25.334145065486414 24.85752248853004 25.74085991138848 62-63 20.98898606012065 30.514030157547023 22.29547359015551 26.201510192176812 64-65 20.508659762313712 32.171676720725564 21.35565442432604 25.964009092634686 66-67 20.624169556340537 31.68070222547327 21.090143935851554 26.60498428233464 68-69 20.3318070916995 32.3351034032565 21.17903323626498 26.154056268779023 70-71 20.585974935069142 31.605933360802595 21.123940388612912 26.684151315515347 72-73 20.53319691294867 29.97560173889694 21.372089685600397 28.11911166255399 74-75 20.34129787637906 29.628840874263307 21.959592405520397 28.070268843837244 76 20.90750420140834 29.712174593400896 21.740378427677907 27.63994277751286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 1.0 16 8.5 17 15.0 18 13.0 19 10.5 20 14.5 21 19.0 22 26.5 23 59.0 24 96.5 25 109.0 26 228.5 27 374.5 28 496.0 29 591.0 30 760.0 31 1087.0 32 1630.5 33 2016.0 34 2288.0 35 2928.5 36 3554.0 37 3811.0 38 4539.0 39 8404.5 40 13355.5 41 14481.5 42 13794.0 43 12736.0 44 11356.5 45 10734.0 46 10433.0 47 10146.5 48 9477.0 49 8396.0 50 7698.0 51 7243.0 52 6014.5 53 4933.5 54 4626.0 55 4583.5 56 4214.5 57 3738.5 58 3589.0 59 3647.0 60 3877.5 61 4219.5 62 4389.0 63 4564.5 64 4938.5 65 5249.0 66 5656.5 67 5952.0 68 5899.0 69 5812.5 70 5544.0 71 5309.0 72 5076.0 73 4421.5 74 3495.0 75 2990.0 76 2493.0 77 1610.5 78 912.0 79 599.0 80 419.0 81 163.5 82 53.5 83 19.0 84 10.5 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 215999.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.56221556581281 #Duplication Level Percentage of deduplicated Percentage of total 1 57.92854227195237 22.338529345043266 2 21.21641414747761 16.36303871777184 3 8.24669243883113 9.540321945934934 4 3.6053017024035343 5.56113685711508 5 1.9605253679736836 3.7801100931022824 6 1.2497899008331932 2.8916800540743246 7 0.9304391672869594 2.5115857017856564 8 0.7527553005018369 2.3222329733008023 9 0.6338992004225994 2.200010185232339 >10 3.4156121689437415 21.77324895022662 >50 0.044420966696280643 1.1254681734637662 >100 0.009604533339736356 0.7004662058620641 >500 0.0012005666674670444 0.277779063791962 >1k 0.0036017000024011333 3.954647938184899 >5k 0.0 0.0 >10k+ 0.0012005666674670444 4.659743795110162 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 10065 4.659743795110162 TruSeq Adapter, Index 5 (95% over 23bp) CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG 3182 1.4731549683100384 TruSeq Adapter, Index 5 (96% over 29bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 2933 1.3578766568363743 RNA PCR Primer, Index 5 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 2427 1.1236163130384864 TruSeq Adapter, Index 5 (95% over 22bp) TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 600 0.277779063791962 RNA PCR Primer, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 380 0.17592674040157594 TruSeq Adapter, Index 5 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT 297 0.1375006365770212 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006018546382159176 0.0 2 0.0 0.0 0.0 0.0074074417011189865 0.0 3 0.0 0.0 0.0 0.00833337191375886 0.0 4 0.0 0.0 0.0 0.03981499914351455 0.0 5 0.0 0.0 0.0 0.043981685100393984 0.0 6 0.0 0.0 0.0 0.044907615313033854 0.0 7 0.0 0.0 0.0 0.045370580419353795 0.0 8 0.0 0.0 0.0 0.04583354552567373 0.0 9 0.0 0.0 0.0 0.047685405950953476 0.0 10 0.0 0.0 0.0 0.05277802212047278 0.0 11 0.0 0.0 0.0 0.05324098722679272 0.0 12 0.0 0.0 0.0 0.05324098722679272 0.0 13 0.0 0.0 0.0 0.05648174297103227 0.0 14 0.0 0.0 0.0 0.05833360339631202 0.0 15 0.0 0.0 0.0 0.06157435914055158 0.0 16 0.0 0.0 0.0 0.062037324246871514 0.0 17 0.0 0.0 0.0 0.06296325445951138 0.0 18 0.0 0.0 0.0 0.06388918467215127 0.0 19 0.0 0.0 0.0 0.06990773105431043 0.0 20 0.0 0.0 0.0 0.0745373821175098 0.0 21 0.0 0.0 0.0 0.07685220764910948 0.0 22 0.0 0.0 0.0 0.07870406807438923 0.0 23 0.0 0.0 0.0 0.08425964935022848 0.0 24 0.0 0.0 0.0 0.08888930041342784 0.0 25 0.0 0.0 0.0 0.09259302126398733 0.0 26 0.0 0.0 0.0 0.10740790466622531 0.0 27 0.0 0.0 0.0 0.1703711591257367 0.0 28 0.0 0.0 0.0 0.24537150634956642 0.0 29 0.0 0.0 0.0 0.3560201667600313 0.0 30 0.0 0.0 0.0 0.4694466178084158 0.0 31 0.0 0.0 0.0 0.5916694058768791 0.0 32 0.0 0.0 0.0 0.716207019476942 0.0 33 0.0 0.0 0.0 0.869911434775161 0.0 34 0.0 0.0 0.0 1.0708382909180134 0.0 35 0.0 0.0 0.0 1.4074139232126075 0.0 36 0.0 0.0 0.0 1.8398233325154283 0.0 37 0.0 0.0 0.0 2.4439927962629455 0.0 38 0.0 0.0 0.0 3.0384399927777443 0.0 39 0.0 0.0 0.0 3.6551095143959 0.0 40 0.0 0.0 0.0 4.271779036014055 0.0 41 0.0 0.0 0.0 4.922707975499886 0.0 42 0.0 0.0 0.0 5.662063250292825 0.0 43 0.0 0.00138889531895981 0.0 6.369473932749688 0.0 44 0.0 0.00138889531895981 0.0 7.055588220315835 0.0 45 0.0 0.00138889531895981 0.0 7.7523507053273395 0.0 46 0.0 0.00138889531895981 0.0 8.502817142671956 0.0 47 0.0 0.00138889531895981 0.0 9.21022782512882 0.0 48 0.0 0.00138889531895981 0.0 9.853286357807212 0.0 49 0.0 0.00138889531895981 0.0 10.517178320270002 0.0 50 0.0 0.00138889531895981 0.0 11.156996097204154 0.0 51 0.0 0.00138889531895981 0.0 11.794036083500387 0.0 52 0.0 0.00138889531895981 0.0 12.42181676767022 0.0 53 0.0 0.00138889531895981 0.0 13.062560474817014 0.0 54 0.0 0.00138889531895981 0.0 13.695896740262686 0.0 55 0.0 0.00138889531895981 0.0 14.295899518053325 0.0 56 0.0 0.00138889531895981 0.0 14.91951351626628 0.0 57 0.0 0.00138889531895981 0.0 15.528775596183316 0.0 58 0.0 0.00138889531895981 0.0 16.126000583336033 0.0 59 0.0 0.0023148255315996835 0.0 16.704244001129634 0.0 60 0.0 0.0023148255315996835 0.0 17.288505965305394 0.0 61 0.0 0.0023148255315996835 0.0 17.91536071926259 0.0 62 0.0 0.0023148255315996835 0.0 18.502400474076268 0.0 63 0.0 0.0023148255315996835 0.0 19.093143949740508 0.0 64 0.0 0.0023148255315996835 0.0 19.7362024824189 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATA 15 0.0022224518 70.0 3 AATGCGT 25 2.386677E-4 55.999996 6 AAATGCG 25 2.386677E-4 55.999996 5 TCTTTAT 90 0.0 54.444443 1 TTGCTCC 20 0.0069444324 52.5 67 ATCGTAT 20 0.0069444324 52.5 39 CTTTATA 100 0.0 48.999996 2 ATAAATG 30 5.8687443E-4 46.666664 3 AAAGCAG 30 5.8687443E-4 46.666664 7 ATAAGAC 35 0.001253506 40.0 3 TTAAATG 35 0.001253506 40.0 24 TAAGACA 35 0.001253506 40.0 4 GCCTAAA 45 9.1770984E-5 38.888885 2 TTAATAC 40 0.0024150996 35.0 3 CAGTTAT 40 0.0024150996 35.0 34 TAAATGT 40 0.0024150996 35.0 25 CTCTATA 50 1.7058967E-4 35.0 1 TGTGTAC 40 0.0024150996 35.0 66 AATGTCC 50 1.7058967E-4 35.0 27 TCAGTTA 40 0.0024150996 35.0 33 >>END_MODULE