FastQCFastQC Report
Wed 25 May 2016
SRR1780331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781155
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGG300.0057473429.32961510
TAAGACA300.005763560529.31270831
CTCGTAA454.1051782E-529.2939451
CGGTAAG655.94986E-727.0648228
CGTAAGG609.854255E-625.6305583
CAGACTA450.001408755124.4116211
TTTAACG500.002562367622.02544434
TTCGGTA703.2245793E-521.99016626
TATCCAC602.8989842E-421.969053
CAATGTG856.1495684E-620.68999732
ATTAGAG755.5290526E-520.5044483
GGCTATC654.974882E-420.2804241
GGCTTAT550.00445232520.0231335
TTAACGG550.00445232520.0231335
GATACTC550.00451553119.9744227
TCGGTAA901.0050862E-519.54681427
CAAGGAA901.01345795E-519.5280464
ATAGTGA708.19349E-418.8318216
TAACGGC600.00736056418.35453636
CATTGAA600.007428942718.32513626
Produced by FastQC (version 0.11.5)