FastQCFastQC Report
Wed 25 May 2016
SRR1780331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781155
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT218522.797396163373466No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC71250.9121109126869827No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG54220.6941004026089572No Hit
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG41700.5338249131094341TruSeq Adapter, Index 9 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC11520.14747393283023216No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT8340.10676498262188683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT6100.036.4268141
CGTATGG250.002350623335.205072
TCTTCTG6400.035.0630644
CGTCTTC6600.033.66720642
CTTTATA1850.033.3020942
GTCTTCT6800.032.67699443
TCTTTAT2050.031.1843341
ATGCCGT31650.031.141144
CCTTATA300.005687842629.3921321
TATCTAG300.005743334529.3338035
GATAATT300.005746929429.33004811
GCCGTCT7850.028.86670340
TATGCCG36450.027.04021343
TTATCGG508.3368126E-526.40042125
TATCGGA508.340445E-526.39873326
ATTAGAG450.001396598624.4479663
ATCGATA450.001397640924.44483623
TTTAGCG450.001398162324.44327226
CATCGTT450.001398683824.44170632
TTAATAC551.591359E-424.0034562