FastQCFastQC Report
Wed 25 May 2016
SRR1780330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116110
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAAC207.898682E-570.039
AGGTAAC252.3820634E-456.00000451
GGTAACG252.3820634E-456.00000452
TCTAGGT252.3820634E-456.00000448
TTGGCAA200.006936385852.528
CGTATCC200.006936385852.556
ATCGTGA200.006936385852.58
TGGCAAC200.006936385852.529
CTTGACT200.006936385852.536
TTTGGCA200.006936385852.527
GTATCGA200.006936385852.56
GGGTTCC200.006936385852.528
AATAAGT200.006936385852.54
TTGGGAC200.006936385852.554
CTAGGTA305.8574695E-446.66666849
ACCTGGT350.001251112240.0000042
ACGCTTC350.001251112240.00000415
GACGAAT459.150885E-538.88888523
AGATCTT400.002410516635.063
CCAGGCA400.002410516635.037
Produced by FastQC (version 0.11.5)