FastQCFastQC Report
Wed 25 May 2016
SRR1780330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116110
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT73356.3172853328739995No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG19821.7070019808801997No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC18941.6312117819309275No Hit
CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG15491.334079751959349RNA PCR Primer, Index 15 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC4750.4090948238739127No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT2230.19205925415554218RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT1680.144690379812247No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGG150.002219855870.0000136
TTTTGGC150.002219855870.0000137
GCTTATA207.898682E-570.03
CAGCTTA207.898682E-570.01
TCTTTAT800.056.8751
GAATGCG200.006936385852.541
CGTTGCG200.006936385852.520
GCTGCCA200.006936385852.58
TGTATAA200.006936385852.514
GTGTATA200.006936385852.513
ATGCGCC200.006936385852.510
CTCTGCG200.006936385852.544
GAATTCG200.006936385852.564
CTTTATA900.050.5555532
CAATCGG350.001251112240.00000422
TTTATAC1450.036.2068983
CTCAGCG501.7010474E-435.00000461
TCTTCTG17000.033.9705950
GTCTTCT16950.033.86430449
TTCTGCT16950.033.86430452
Produced by FastQC (version 0.11.5)