##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780330_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116110 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76194126259581 34.0 34.0 34.0 34.0 34.0 2 33.76691930066316 34.0 34.0 34.0 34.0 34.0 3 33.75880630436655 34.0 34.0 34.0 34.0 34.0 4 33.78329170614073 34.0 34.0 34.0 34.0 34.0 5 33.7751614847989 34.0 34.0 34.0 34.0 34.0 6 37.53605201963655 38.0 38.0 38.0 37.0 38.0 7 37.5223236585996 38.0 38.0 38.0 37.0 38.0 8 37.445276031349586 38.0 38.0 38.0 37.0 38.0 9 37.475927999311 38.0 38.0 38.0 37.0 38.0 10-11 37.411282404616315 38.0 38.0 38.0 37.0 38.0 12-13 37.38828266299199 38.0 38.0 38.0 37.0 38.0 14-15 37.30866850400483 38.0 38.0 38.0 36.0 38.0 16-17 37.34079751959349 38.0 38.0 38.0 36.5 38.0 18-19 37.30676513650849 38.0 38.0 38.0 36.0 38.0 20-21 37.34465592972182 38.0 38.0 38.0 36.5 38.0 22-23 37.32372749978468 38.0 38.0 38.0 36.5 38.0 24-25 37.33496253552666 38.0 38.0 38.0 36.5 38.0 26-27 37.23706399104297 38.0 38.0 38.0 36.0 38.0 28-29 37.20890534837654 38.0 38.0 38.0 36.0 38.0 30-31 37.22725432779261 38.0 38.0 38.0 36.0 38.0 32-33 37.175949530617515 38.0 38.0 38.0 36.0 38.0 34-35 37.15785892687968 38.0 38.0 38.0 36.0 38.0 36-37 37.153178020842304 38.0 38.0 38.0 36.0 38.0 38-39 36.645719576263886 38.0 37.5 38.0 34.0 38.0 40-41 36.91272069589183 38.0 37.5 38.0 35.5 38.0 42-43 37.11080871587288 38.0 38.0 38.0 36.0 38.0 44-45 37.16198863147016 38.0 38.0 38.0 36.0 38.0 46-47 37.10714408750323 38.0 38.0 38.0 36.0 38.0 48-49 37.12179398845922 38.0 38.0 38.0 36.0 38.0 50-51 37.0072216002067 38.0 38.0 38.0 36.0 38.0 52-53 37.01027473947119 38.0 38.0 38.0 36.0 38.0 54-55 36.970226509344585 38.0 38.0 38.0 35.5 38.0 56-57 37.01769873395918 38.0 38.0 38.0 36.0 38.0 58-59 36.921483937645334 38.0 38.0 38.0 35.5 38.0 60-61 36.9234131427095 38.0 38.0 38.0 35.5 38.0 62-63 36.89532770648523 38.0 38.0 38.0 35.0 38.0 64-65 36.82589785548187 38.0 38.0 38.0 35.5 38.0 66-67 36.549905262251315 38.0 37.5 38.0 34.0 38.0 68-69 36.45902592369305 38.0 37.0 38.0 34.0 38.0 70-71 35.812358969942295 38.0 37.0 38.0 31.0 38.0 72-73 34.73535871156662 38.0 37.0 38.0 23.5 38.0 74-75 34.41937817586771 38.0 37.0 38.0 22.5 38.0 76 33.76989923348549 38.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06167458456666708 1101 2 -0.0597151970807559 1101 3 0.018745133754791254 1101 4 0.031174517212043895 1101 5 -0.05304812473117693 1101 6 0.17259939064943097 1101 7 0.1073320084199878 1101 8 -0.05016081824838636 1101 9 -0.11713341274463573 1101 10-11 0.007420240528631439 1101 12-13 -0.06339352635271212 1101 14-15 0.16788964286166674 1101 16-17 0.10267031000647364 1101 18-19 0.0634932722892998 1101 20-21 -0.002227293581483991 1101 22-23 0.20305427870373194 1101 24-25 -0.05654918549950594 1101 26-27 0.009083653129202673 1101 28-29 -0.17863343444282975 1101 30-31 0.08871773684064976 1101 32-33 0.02225154630379933 1101 34-35 0.07293605095424027 1101 36-37 0.11245614760951383 1101 38-39 0.38407505971792233 1101 40-41 0.19952139736278696 1101 42-43 0.19676449418022912 1101 44-45 0.11070648178399978 1101 46-47 0.110583173811996 1101 48-49 0.11090137548507073 1101 50-51 0.020554921350573352 1101 52-53 0.03437969235449856 1101 54-55 0.1720281266722239 1101 56-57 0.18592750536488722 1101 58-59 0.03463746654175992 1101 60-61 -0.13596519025181664 1101 62-63 -0.017049468927794464 1101 64-65 -0.07782406554872523 1101 66-67 -0.2891487167649771 1101 68-69 0.06528088511860375 1101 70-71 -0.22882982932085838 1101 72-73 0.07702268619635078 1101 74-75 0.0028162347419069533 1101 76 -0.6909244798179159 1102 1 0.031870254592902825 1102 2 0.051075908789322 1102 3 0.09745886386942715 1102 4 0.032297055281958365 1102 5 -0.1192438545510015 1102 6 -0.24466728584416586 1102 7 -0.05876960962226008 1102 8 -0.12075599020080574 1102 9 -0.13662475559497267 1102 10-11 -0.1918529444180166 1102 12-13 -0.06393211785091069 1102 14-15 0.07500245226831481 1102 16-17 -0.008653717977608721 1102 18-19 0.17571306297563893 1102 20-21 0.12252750555223457 1102 22-23 0.10350556168536684 1102 24-25 0.05946342321093567 1102 26-27 0.12668368941326236 1102 28-29 0.15348476087879703 1102 30-31 0.1277741245358186 1102 32-33 0.1648308893355619 1102 34-35 0.192786832762323 1102 36-37 0.15498786781406437 1102 38-39 0.08962992234874179 1102 40-41 0.2901238261269725 1102 42-43 0.18819238528269722 1102 44-45 0.1322614804800324 1102 46-47 -0.11175873540982906 1102 48-49 0.12353276346371445 1102 50-51 -0.16200491899956404 1102 52-53 0.02937929549760554 1102 54-55 -0.15301467717841888 1102 56-57 -0.17572251221019997 1102 58-59 0.16650167345161293 1102 60-61 0.08497071169052361 1102 62-63 0.1280754276059497 1102 64-65 0.12274173899358942 1102 66-67 0.20238689263712928 1102 68-69 0.23399041988893288 1102 70-71 0.05650460765259879 1102 72-73 0.43457940838518994 1102 74-75 0.3693702696426371 1102 76 0.7276461550397215 1103 1 -0.09804370203956125 1103 2 -0.08165725196819551 1103 3 -0.02201670128143718 1103 4 -0.15174771661403952 1103 5 -0.01763722260911038 1103 6 0.0533823279563137 1103 7 -0.08506471426818507 1103 8 0.04211854997546993 1103 9 -0.008618215879828028 1103 10-11 -0.01921599293139309 1103 12-13 -0.10909178541538722 1103 14-15 -4.924805004833388E-4 1103 16-17 -0.00782053311537112 1103 18-19 0.021961635033029836 1103 20-21 0.021916503319921787 1103 22-23 0.07721045703944895 1103 24-25 0.015246983792387425 1103 26-27 0.08697902462606777 1103 28-29 -0.05275731338469569 1103 30-31 0.029625995795655058 1103 32-33 0.034078507684490944 1103 34-35 -0.1361325421918309 1103 36-37 -0.14336043489928585 1103 38-39 0.06582913213316033 1103 40-41 -0.11021363994301225 1103 42-43 -0.20935731760043552 1103 44-45 0.12321173043549294 1103 46-47 -0.014889204823028024 1103 48-49 0.11691444597311573 1103 50-51 -0.22503351977301378 1103 52-53 -0.07450156430725485 1103 54-55 -0.2665007436026414 1103 56-57 -0.2874793270335516 1103 58-59 0.055373677392204 1103 60-61 0.03755253673217851 1103 62-63 -0.14465899078909672 1103 64-65 0.04239966158600339 1103 66-67 -0.22966399976149887 1103 68-69 -0.6039787051097605 1103 70-71 -0.6715522375636667 1103 72-73 -0.40444822819948456 1103 74-75 -0.4151702210455994 1103 76 -0.6563210625420481 1104 1 0.039665199168162246 1104 2 0.059042879727620345 1104 3 0.09766314517553809 1104 4 0.11426761727057766 1104 5 0.17397508125090866 1104 6 0.08782741896934709 1104 7 -0.041513215079795884 1104 8 0.06473143574721973 1104 9 -0.012712015967622392 1104 10-11 0.14346406791412392 1104 12-13 -0.1663845686889971 1104 14-15 -0.006602553698847657 1104 16-17 0.11717819076240943 1104 18-19 0.16561726579472236 1104 20-21 0.09357331832304538 1104 22-23 0.19357749020841908 1104 24-25 0.09988961852536704 1104 26-27 0.21554068174733487 1104 28-29 0.09785573573069684 1104 30-31 0.001377754292079203 1104 32-33 -0.2954417724498555 1104 34-35 0.23037996891043377 1104 36-37 0.03835936949669616 1104 38-39 0.22952498626729323 1104 40-41 0.14787952718815944 1104 42-43 0.06355928526011922 1104 44-45 -0.13047115829685652 1104 46-47 -0.049125011791680606 1104 48-49 -0.15413391395460962 1104 50-51 -0.07783026923448944 1104 52-53 0.13467018132585906 1104 54-55 -0.1295115753280598 1104 56-57 -0.29696346738658264 1104 58-59 -0.03208663520668864 1104 60-61 0.15692611385589572 1104 62-63 0.026203565743116997 1104 64-65 -0.023647320129725813 1104 66-67 -0.4091990829136236 1104 68-69 -0.09147270733531343 1104 70-71 0.013148588342637879 1104 72-73 0.2703856206872288 1104 74-75 0.2849053253899356 1104 76 0.45140407094033463 1105 1 0.007949988812171682 1105 2 0.12560343996954515 1105 3 -0.09751962314108908 1105 4 -0.0638850698746225 1105 5 0.045681803092911366 1105 6 0.09261281872529992 1105 7 0.10570944925252945 1105 8 -0.11204186787631443 1105 9 0.05893104201953747 1105 10-11 0.09849616510760484 1105 12-13 0.07105508328193366 1105 14-15 -0.02863024747570364 1105 16-17 -0.10310935764864837 1105 18-19 -0.024483004776662654 1105 20-21 0.10130317136564315 1105 22-23 0.04175511236343965 1105 24-25 -0.10366494670355308 1105 26-27 0.038088297005437255 1105 28-29 0.1892398940414708 1105 30-31 0.012927438625467857 1105 32-33 0.06036490164425601 1105 34-35 -0.05128484089379981 1105 36-37 0.036072776043461374 1105 38-39 -0.06983306986300875 1105 40-41 0.09839679600878526 1105 42-43 4.684051073269302E-4 1105 44-45 0.12458216018158197 1105 46-47 0.0191491672098536 1105 48-49 0.06402620064033471 1105 50-51 -0.14395579313495688 1105 52-53 0.0859938589745397 1105 54-55 0.06708771375889455 1105 56-57 0.1981078891840582 1105 58-59 0.11754464496731742 1105 60-61 -0.07776007639292004 1105 62-63 -0.06615146635407143 1105 64-65 -0.09157665325083997 1105 66-67 -0.318891904813988 1105 68-69 0.14453450790157518 1105 70-71 0.06978819787099155 1105 72-73 0.3637009159283551 1105 74-75 0.2380593388654475 1105 76 0.2385333616188845 1106 1 -0.13415527434572283 1106 2 0.00682647707049 1106 3 -0.07886129699785727 1106 4 0.09972580156627231 1106 5 0.015548067113620334 1106 6 -0.17837510189592365 1106 7 -0.04684663357750196 1106 8 0.19830110019961467 1106 9 0.13963639145870843 1106 10-11 0.11502981480562369 1106 12-13 0.12193407379098886 1106 14-15 -0.24146458742393406 1106 16-17 -0.0069488744734940155 1106 18-19 0.06372342317847313 1106 20-21 -0.010630607754286814 1106 22-23 -0.002992514901443144 1106 24-25 -0.01558794066387037 1106 26-27 -0.18463603431466424 1106 28-29 -0.04617317757197981 1106 30-31 0.06808274492399846 1106 32-33 -0.07372698791570542 1106 34-35 -0.20057302035885982 1106 36-37 -0.08498524811529506 1106 38-39 0.08518202936045327 1106 40-41 -0.039588536174129274 1106 42-43 -0.07641899782623085 1106 44-45 -0.2921024817511224 1106 46-47 -0.14154431855373417 1106 48-49 -0.0322637044502585 1106 50-51 -0.0604301244550598 1106 52-53 -0.040273397280856216 1106 54-55 0.11499000021273531 1106 56-57 -0.06753598484527856 1106 58-59 -0.0023270116331985946 1106 60-61 -0.05680451125055441 1106 62-63 -0.0304094473721932 1106 64-65 -0.07429882753902461 1106 66-67 -0.06814772880019149 1106 68-69 -0.004786027042349872 1106 70-71 -0.14114795398405988 1106 72-73 0.11562067434077505 1106 74-75 -0.011509254749732634 1106 76 -0.3895727643517759 1107 1 0.1040538849160697 1107 2 0.02276120024205852 1107 3 -0.006663757695058337 1107 4 0.049059362741935786 1107 5 0.09270117716146586 1107 6 0.06709653173360408 1107 7 0.20678460360637274 1107 8 0.11187765522353743 1107 9 0.134894422900949 1107 10-11 0.11285107249530313 1107 12-13 0.15254326722913447 1107 14-15 0.16002208379957494 1107 16-17 0.1478699908716834 1107 18-19 -0.12332801222822098 1107 20-21 0.23144475109739204 1107 22-23 0.12717665803089062 1107 24-25 0.21021093151658476 1107 26-27 0.14076553779744216 1107 28-29 0.1590344437540594 1107 30-31 -0.2591558059987591 1107 32-33 0.18468893826337762 1107 34-35 -0.002093867932330795 1107 36-37 -0.029170996577356334 1107 38-39 0.1302520908778888 1107 40-41 0.37710661078417473 1107 42-43 0.0755375982093014 1107 44-45 -0.026338640329385044 1107 46-47 -0.061849342477181324 1107 48-49 0.02933916636312972 1107 50-51 0.0032019671375493886 1107 52-53 0.18065534502946434 1107 54-55 0.23531211231115634 1107 56-57 -0.05102453576803612 1107 58-59 0.17216461516105852 1107 60-61 -0.1251999316196546 1107 62-63 0.006586451465807386 1107 64-65 0.40109420048558775 1107 66-67 0.14481365192435902 1107 68-69 -0.12273715486189474 1107 70-71 -0.05075150832445274 1107 72-73 0.12262682608376707 1107 74-75 0.01600270697274908 1107 76 -0.14837317446797726 1108 1 -0.15598443741733092 1108 2 -0.17275721576816494 1108 3 0.02707054079333915 1108 4 -0.027819496104136476 1108 5 0.05879655719127186 1108 6 -0.2221412796353519 1108 7 0.05325043285908038 1108 8 0.15353190261549088 1108 9 0.14745563218347257 1108 10-11 -0.12117596603993519 1108 12-13 -0.13714163802954715 1108 14-15 0.13530417875379896 1108 16-17 -0.06048640682897144 1108 18-19 0.06404158538727955 1108 20-21 0.09966382710334898 1108 22-23 -0.03529406796442913 1108 24-25 0.11764652870628112 1108 26-27 -0.15043629315849216 1108 28-29 0.09579727109065317 1108 30-31 -0.16576108596469652 1108 32-33 -0.13635640983115138 1108 34-35 0.061829913204562104 1108 36-37 -0.027861650186046916 1108 38-39 -0.1993412665843266 1108 40-41 -0.06717697448301863 1108 42-43 -0.08149880800743148 1108 44-45 0.0655657667389562 1108 46-47 0.14209998688198766 1108 48-49 0.13943603670590932 1108 50-51 0.006863878996185235 1108 52-53 -0.005809419713983743 1108 54-55 0.05889099244741658 1108 56-57 -0.05271178294708534 1108 58-59 0.02738071054108815 1108 60-61 0.06158418590215575 1108 62-63 -0.02024982700981326 1108 64-65 0.24121023199505487 1108 66-67 0.3663539968253531 1108 68-69 0.4396164751146827 1108 70-71 0.36650950934640036 1108 72-73 0.08173370281360093 1108 74-75 0.16592819132446124 1108 76 0.251648115717245 1109 1 -0.12431838179485766 1109 2 -0.002621563958598472 1109 3 -0.05080093042647604 1109 4 -0.06357081857834146 1109 5 0.04659313185214842 1109 6 -0.297027363540451 1109 7 -0.03161836722471634 1109 8 -0.07930735784646004 1109 9 0.09958468471581483 1109 10-11 -0.152972959702538 1109 12-13 -0.03992799952328596 1109 14-15 -0.20878004059360222 1109 16-17 0.010749124517587916 1109 18-19 0.052136698779619906 1109 20-21 0.054092151620181994 1109 22-23 -0.043464601667878355 1109 24-25 -0.005272865836225549 1109 26-27 -0.039657473695861256 1109 28-29 -0.21962723003540674 1109 30-31 0.12750346125156398 1109 32-33 -0.1389594580687259 1109 34-35 -0.22039393846883115 1109 36-37 0.0837185100821145 1109 38-39 -0.0011324989731562596 1109 40-41 0.023573562362948053 1109 42-43 -0.1065917740159179 1109 44-45 0.012368259799238501 1109 46-47 -0.13003116065899434 1109 48-49 0.05021485616853738 1109 50-51 0.028138691754790557 1109 52-53 0.09743784368097863 1109 54-55 -0.04472813498148298 1109 56-57 0.041705019022217016 1109 58-59 -0.018149064769232837 1109 60-61 0.07251489349133067 1109 62-63 0.11300319765891231 1109 64-65 -0.027741739931826714 1109 66-67 -0.06109585390527883 1109 68-69 -0.20643181483450945 1109 70-71 0.3221738889619701 1109 72-73 -0.7566468555062045 1109 74-75 -0.3084236653769423 1109 76 -0.23892958291575894 1110 1 -0.011722142335365504 1110 2 0.033800161281014596 1110 3 0.036906606367111294 1110 4 0.024639520289305494 1110 5 0.05879655719127186 1110 6 -0.064764230455026 1110 7 -0.009044649108126634 1110 8 0.09779419769745346 1110 9 0.009750714150683848 1110 10-11 -0.027733343089110463 1110 12-13 0.144825575085207 1110 14-15 -0.14830237862324935 1110 16-17 0.05590703579397882 1110 18-19 0.031254700141367664 1110 20-21 0.21441792546400507 1110 22-23 0.16306658777327243 1110 24-25 0.09797439755873683 1110 26-27 -0.05043629315849074 1110 28-29 0.21874809076279433 1110 30-31 0.1375176025598961 1110 32-33 0.0800370327918003 1110 34-35 0.14707581484390175 1110 36-37 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0.2312857266077586 2115 60-61 -0.06453094943905313 2115 62-63 0.21611529471558555 2115 64-65 0.4450008752062118 2115 66-67 0.23893174313547405 2115 68-69 0.3303359348391055 2115 70-71 0.2558401558253536 2115 72-73 -0.062098332478598195 2115 74-75 0.1318287414034316 2115 76 0.33894183610284756 2116 1 -0.09041066692552846 2116 2 -0.10390475675176702 2116 3 -0.008995032977153983 2116 4 0.021360831764717148 2116 5 -0.08546573789069356 2116 6 0.02048167118432076 2116 7 0.04669305580990368 2116 8 0.009269607533518354 2116 9 -0.08533125306243505 2116 10-11 -0.13101203161370734 2116 12-13 -0.08468262163610518 2116 14-15 -0.028630247475710746 2116 16-17 -0.38179788223881417 2116 18-19 -0.17202398838320931 2116 20-21 -0.364270599126165 2116 22-23 -0.14021210075131307 2116 24-25 -0.018419045064213435 2116 26-27 -0.047157604633902395 2116 28-29 0.13514153338574175 2116 30-31 -0.006744692522076434 2116 32-33 -0.11340559015901874 2116 34-35 -0.06276025072986613 2116 36-37 -0.15245181412047515 2116 38-39 -0.05016093871547156 2116 40-41 -0.22619336792564582 2116 42-43 0.04145201166470258 2116 44-45 -0.26558177424464446 2116 46-47 0.08308359343936189 2116 48-49 -0.20974429116294857 2116 50-51 -0.23411972756118615 2116 52-53 -0.33531761643529734 2116 54-55 -0.1443876960771675 2116 56-57 -0.1822199796684032 2116 58-59 -0.02507830585235382 2116 60-61 -0.12038302721258987 2116 62-63 -0.14647933520653567 2116 64-65 -0.3161668171852625 2116 66-67 -0.24512141301071466 2116 68-69 -0.20136713144269436 2116 70-71 -0.04660524475195871 2116 72-73 -0.17892203489132186 2116 74-75 0.4396986831277445 2116 76 0.25492680424184044 2117 1 0.0024705367573787385 2117 2 0.06599194210293291 2117 3 -0.005952140545083751 2117 4 -0.0036447824279619567 2117 5 0.12072110244167078 2117 6 0.02431055059145848 2117 7 0.03755315012833904 2117 8 -0.2850712862459517 2117 9 -0.013166420365372744 2117 10-11 -0.056158000623362625 2117 12-13 -0.11019912736257709 2117 14-15 0.02232416527973413 2117 16-17 0.07506266121504268 2117 18-19 -0.015606966151011648 2117 20-21 -0.1037046885041022 2117 22-23 0.12277352691542376 2117 24-25 -0.07941724852255305 2117 26-27 0.08361378544020681 2117 28-29 -0.5414192121790435 2117 30-31 -0.07106649804643439 2117 32-33 0.019504807325823492 2117 34-35 -0.18137691365373598 2117 36-37 -0.01502760572163453 2117 38-39 -0.0026424118796271046 2117 40-41 0.12470490290301228 2117 42-43 0.06259506129416792 2117 44-45 -0.06801271967469802 2117 46-47 0.10969486673826623 2117 48-49 0.05362310160597872 2117 50-51 0.15944640762857176 2117 52-53 -0.1657465407559755 2117 54-55 -0.05513887505762938 2117 56-57 0.1507634023212674 2117 58-59 0.13729537481237486 2117 60-61 0.031200175122911844 2117 62-63 0.05115147548288235 2117 64-65 -0.1830767655346932 2117 66-67 -0.42868305684633157 2117 68-69 -0.08188588284624387 2117 70-71 -0.18918328203019996 2117 72-73 -0.6633987921336271 2117 74-75 -0.1636361473236363 2117 76 -1.224738589874491 2118 1 0.00929679015897733 2118 2 -0.26114933366847737 2118 3 -0.1265531275844296 2118 4 -0.04738123369865832 2118 5 -0.08322474876445796 2118 6 -0.04221082757375427 2118 7 -0.0761639293421652 2118 8 0.03191132525720519 2118 9 0.03292232092884717 2118 10-11 0.13623972088394964 2118 12-13 0.15266351734938155 2118 14-15 0.24203266581015725 2118 16-17 0.11191520491689744 2118 18-19 0.07490431100409722 2118 20-21 0.12412151877415312 2118 22-23 0.02382167967590476 2118 24-25 -0.005337409031760387 2118 26-27 -0.004556732524832796 2118 28-29 0.18410659382620764 2118 30-31 0.23898031713736145 2118 32-33 0.19130269487713036 2118 34-35 0.3104361945977274 2118 36-37 0.24848653108016805 2118 38-39 -0.12371616309036426 2118 40-41 0.02405225772982078 2118 42-43 0.21598973866144178 2118 44-45 0.039137550146804756 2118 46-47 0.2319217012938708 2118 48-49 0.1644401764641401 2118 50-51 0.21339917733476454 2118 52-53 0.1362229255970533 2118 54-55 0.42566805266709906 2118 56-57 0.23667387693037512 2118 58-59 -0.02733033433388954 2118 60-61 0.2823878509681208 2118 62-63 0.2063219012012638 2118 64-65 0.3377825121738951 2118 66-67 0.38248801459871373 2118 68-69 0.4016134944887497 2118 70-71 0.2600349274619873 2118 72-73 0.5124175908745414 2118 74-75 0.2702114611815105 2118 76 0.18616266006785764 2119 1 0.039075816626741755 2119 2 0.025190889757837454 2119 3 0.01180618296031355 2119 4 0.0725821975230474 2119 5 -0.04200683006309447 2119 6 -0.08335070315071391 2119 7 -0.09671156909509904 2119 8 0.053629611876154115 2119 9 -0.03178657821713671 2119 10-11 0.04577659068571904 2119 12-13 0.13861018044836015 2119 14-15 -0.3120902409617585 2119 16-17 0.04655408823127516 2119 18-19 -0.021356308435322546 2119 20-21 -0.11917234703626889 2119 22-23 -6.615633503770368E-4 2119 24-25 -0.16715425296780495 2119 26-27 -0.045105710159901946 2119 28-29 0.124018962546522 2119 30-31 -0.07547772910876205 2119 32-33 -0.13596557278848564 2119 34-35 -0.0021153696094629026 2119 36-37 0.0728114580541046 2119 38-39 0.15325888540786536 2119 40-41 0.19328877299282965 2119 42-43 0.013311744592726882 2119 44-45 0.2056537050735514 2119 46-47 0.03387612410922003 2119 48-49 0.0696010567905887 2119 50-51 0.07682370963346585 2119 52-53 0.12190201226951558 2119 54-55 0.11240852583141958 2119 56-57 0.3445740709232865 2119 58-59 -0.02094194715079567 2119 60-61 0.257046133573418 2119 62-63 0.31661913401504904 2119 64-65 -0.1519826895118399 2119 66-67 0.07242825586346413 2119 68-69 0.1323479917795396 2119 70-71 0.13808697107694456 2119 72-73 0.036716875107593694 2119 74-75 -0.2788497915221271 2119 76 -1.423164608199805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 4.0 22 8.0 23 9.0 24 23.0 25 46.0 26 96.0 27 169.0 28 342.0 29 500.0 30 820.0 31 1399.0 32 2115.0 33 3571.0 34 5803.0 35 10154.0 36 16133.0 37 74917.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.1424511239342 17.10188614245112 12.653518215485315 34.102144518129364 2 19.06812505382827 17.85548187063991 36.46197571268625 26.61441736284558 3 19.17233657738352 18.352424425114116 31.71130824218413 30.76393075531823 4 15.69029368702093 21.958487641030057 31.34699853587116 31.00422013607786 5 16.111446042545865 30.07751270347085 37.089828610800105 16.72121264318319 6 30.62871415037465 36.04771337524761 15.735940056842649 17.587632417535097 7 28.47299974162432 23.98845921970545 20.84833347687538 26.69020756179485 8 24.54827318921712 35.11669968133667 16.053742141073123 24.281284988373095 9 23.091034363965203 14.641288433382138 17.074326070105933 45.193351132546724 10-11 27.198777021789684 25.170958573766256 23.104814400137798 24.52545000430626 12-13 25.769959521143743 18.408836448195675 24.662389113771425 31.158814916889156 14-15 23.318404960813023 17.96443028162949 19.762725002153132 38.95443975540436 16-17 28.68831280682112 23.888123331323744 19.046593747308588 28.376970114546552 18-19 30.282921367668592 22.44078890707088 19.356213935061582 27.92007579019895 20-21 25.315218327448108 20.810438377400743 19.357075187322366 34.517268107828784 22-23 24.64258031177332 24.275686848677978 19.697269830333305 31.384463009215395 24-25 28.69520282490742 24.27008870898286 20.239858754629232 26.794849711480495 26-27 29.628369649470333 25.34019464301094 19.813969511669967 25.217466195848765 28-29 24.82473516492981 21.663508741710448 24.48970803548359 29.022048057876155 30-31 23.92731030918956 20.608474722246147 29.757988114718803 25.706226853845493 32-33 26.643269313581946 19.96038239600379 23.975971061924035 29.420377228490224 34-35 26.10541727672035 19.497028679700286 25.316079579708894 29.081474463870467 36-37 24.70028421324606 26.892601843079838 21.854276117474807 26.552837826199294 38-39 22.911463267591078 23.72190164499182 25.749720093015245 27.61691499440186 40-41 23.667642752562227 19.159417793471707 26.84738609938851 30.325553354577554 42-43 27.88777883041943 18.904056498148307 25.83929032813711 27.36887434329515 44-45 23.013091034363963 24.018172422702612 28.149599517698732 24.819137025234692 46-47 26.455516320730343 20.72646628197399 25.52062699164585 27.297390405649814 48-49 26.123934200327277 19.42339161140298 22.851175609335975 31.601498578933768 50-51 23.896735853931617 19.901386616139867 26.35474980621824 29.847127723710276 52-53 20.784600809577125 19.891482215140815 32.63198690896564 26.69193006631642 54-55 21.13297734906554 20.62096288002756 28.844199466023596 29.4018603048833 56-57 25.06803892860219 21.43527689260184 23.864008268021703 29.632675910774264 58-59 22.168633192662128 21.537765911635518 28.976832314184826 27.316768581517525 60-61 25.414262337438636 24.964258031177334 24.065541297045907 25.55593833433813 62-63 21.44216691068814 31.753078976832317 21.07182843854965 25.732925673929895 64-65 20.742830074928946 33.39290328137111 20.247610024976314 25.616656618723628 66-67 20.93618120747567 32.86194126259581 20.130910343639652 26.07096718628886 68-69 20.33330462492464 33.43725777280165 20.405649814830763 25.823787787442942 70-71 20.76393075531823 31.83877357678064 21.218241322883472 26.179054345017654 72-73 20.5176126087331 28.91051589010421 22.24872965291534 28.323141848247353 74-75 20.46636809921626 28.077684953923004 23.27189733873051 28.184049608130223 76 20.506416329342862 28.16294892774093 23.004909137886486 28.32572560502971 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.0 17 4.0 18 4.0 19 7.0 20 8.0 21 6.0 22 17.0 23 45.0 24 55.5 25 49.0 26 111.0 27 205.0 28 297.5 29 358.0 30 428.5 31 594.5 32 885.0 33 1080.0 34 1197.5 35 1529.0 36 1762.0 37 1781.0 38 2236.0 39 4686.0 40 7715.0 41 8244.5 42 7740.0 43 7122.0 44 6084.0 45 5420.0 46 5176.0 47 5132.0 48 4802.5 49 4194.5 50 3872.0 51 3561.0 52 2885.5 53 2377.0 54 2233.0 55 2253.0 56 2065.5 57 1806.5 58 1755.0 59 1740.0 60 1797.0 61 1976.0 62 2083.0 63 2194.0 64 2378.5 65 2524.0 66 2685.0 67 2774.0 68 2837.5 69 2958.0 70 3035.5 71 3056.0 72 2933.5 73 2758.5 74 2479.0 75 2252.0 76 2024.5 77 1585.5 78 1109.0 79 844.0 80 678.5 81 390.0 82 188.5 83 110.0 84 75.5 85 23.5 86 4.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 116110.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.53776591163552 #Duplication Level Percentage of deduplicated Percentage of total 1 65.23734616452299 30.360003445009042 2 18.959933376515224 17.647058823529413 3 6.553159988896086 9.149082766342262 4 2.8685111501804386 5.339764016880545 5 1.6544832053298788 3.849797605718715 6 1.1603590265568613 3.2400310050813883 7 0.7939298602757472 2.586340539143915 8 0.5718515776811326 2.1290155886659203 9 0.5126307023225687 2.147101886142451 >10 1.656333857684834 11.221255705796228 >50 0.016655871194596095 0.5072775816036517 >100 0.0074026094198204875 0.8336921884419946 >500 0.0 0.0 >1k 0.005551957064865365 4.672293514770477 >5k 0.0018506523549551219 6.3172853328739995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 7335 6.3172853328739995 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 1982 1.7070019808801997 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 1894 1.6312117819309275 No Hit CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG 1549 1.334079751959349 RNA PCR Primer, Index 15 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 475 0.4090948238739127 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 223 0.19205925415554218 RNA PCR Primer, Index 48 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT 168 0.144690379812247 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010335027129446214 0.0 2 0.0 0.0 0.0 0.014641288433382138 0.0 3 0.0 0.0 0.0 0.014641288433382138 0.0 4 0.0 0.0 0.0 0.040478856256997674 0.0 5 0.0 0.0 0.0 0.047368874343295155 0.0 6 0.0 0.0 0.0 0.047368874343295155 0.0 7 0.0 0.0 0.0 0.05167513564723107 0.0 8 0.0 0.0 0.0 0.05167513564723107 0.0 9 0.0 0.0 0.0 0.05684264921195418 0.0 10 0.0 0.0 0.0 0.05684264921195418 0.0 11 0.0 0.0 0.0 0.062010162776677286 0.0 12 0.0 0.0 0.0 0.062010162776677286 0.0 13 0.0 0.0 0.0 0.06287141503746448 0.0 14 0.0 0.0 0.0 0.06631642408061321 0.0 15 0.0 0.0 0.0 0.06803892860218758 0.0 16 0.0 0.0 0.0 0.06890018086297477 0.0 17 0.0 0.0 0.0 0.07148393764533632 0.0 18 0.0 0.0 0.0 0.07492894668848506 0.0 19 0.0 0.0 0.0 0.08095771251399535 0.0 20 0.0 0.0 0.0 0.0835414692963569 0.0 21 0.0 0.0 0.0 0.08612522607871846 0.0 22 0.0 0.0 0.0 0.09215399190422875 0.0 23 0.0 0.0 0.0 0.09559900094737749 0.0 24 0.0 0.0 0.0 0.09990526225131341 0.0 25 0.0 0.0 0.0 0.10679528033761089 0.0 26 0.0 0.0 0.0 0.12057531651020584 0.0 27 0.0 0.0 0.0 0.18689174059081906 0.0 28 0.0 0.0 0.0 0.2790457324950478 0.0 29 0.0 0.0 0.0 0.368615967616915 0.0 30 0.0 0.0 0.0 0.47799500473688744 0.0 31 0.0 0.0 0.0 0.5684264921195418 0.0 32 0.0 0.0 0.0 0.7019205925415555 0.0 33 0.0 0.0 0.0 0.8784773060029283 0.0 34 0.0 0.0 0.0 1.15321677719404 0.0 35 0.0 0.0 0.0 1.469296356902937 0.0 36 0.0 0.0 0.0 1.992937731461545 0.0 37 0.0 0.0 0.0 2.5940918094909997 0.0 38 0.0 0.0 0.0 3.192662130738093 0.0 39 0.0 0.0 0.0 3.80845749720093 0.0 40 0.0 0.0 0.0 4.481956765136508 0.0 41 0.0 0.0 0.0 5.155456033072086 0.0 42 0.0 0.0 0.0 5.845319093962622 0.0 43 0.0 0.0 0.0 6.503315821204031 0.0 44 0.0 0.0 0.0 7.1638963052278015 0.0 45 0.0 0.0 0.0 7.8959607268969085 0.0 46 0.0 0.0 0.0 8.603910085263974 0.0 47 0.0 0.0 0.0 9.303246921023169 0.0 48 0.0 0.0 0.0 9.886314701576092 0.0 49 0.0 0.0 0.0 10.52019636551546 0.0 50 0.0 0.0 0.0 11.053311514942727 0.0 51 0.0 0.0 0.0 11.589871673413143 0.0 52 0.0 0.0 0.0 12.120403066058048 0.0 53 0.0 0.0 0.0 12.680217035569719 0.0 54 0.0 0.0 0.0 13.24261476186375 0.0 55 0.0 0.0 0.0 13.760227370596848 0.0 56 0.0 0.0 0.0 14.255447420549478 0.0 57 0.0 0.0 0.0 14.7782275428473 0.0 58 0.0 0.0 0.0 15.293256394798036 0.0 59 0.0 0.0 0.0 15.771251399534924 0.0 60 0.0 0.0 0.0 16.269916458530705 0.0 61 0.0 0.0 0.0 16.809921626044268 0.0 62 0.0 0.0 0.0 17.297390405649814 0.0 63 0.0 0.0 0.0 17.792610455602446 0.0 64 0.0 0.0 0.0 18.33261562311601 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTGG 15 0.0022198558 70.00001 36 TTTTGGC 15 0.0022198558 70.00001 37 GCTTATA 20 7.898682E-5 70.0 3 CAGCTTA 20 7.898682E-5 70.0 1 TCTTTAT 80 0.0 56.875 1 GAATGCG 20 0.0069363858 52.5 41 CGTTGCG 20 0.0069363858 52.5 20 GCTGCCA 20 0.0069363858 52.5 8 TGTATAA 20 0.0069363858 52.5 14 GTGTATA 20 0.0069363858 52.5 13 ATGCGCC 20 0.0069363858 52.5 10 CTCTGCG 20 0.0069363858 52.5 44 GAATTCG 20 0.0069363858 52.5 64 CTTTATA 90 0.0 50.555553 2 CAATCGG 35 0.0012511122 40.000004 22 TTTATAC 145 0.0 36.206898 3 CTCAGCG 50 1.7010474E-4 35.000004 61 TCTTCTG 1700 0.0 33.97059 50 GTCTTCT 1695 0.0 33.864304 49 TTCTGCT 1695 0.0 33.864304 52 >>END_MODULE