FastQCFastQC Report
Wed 25 May 2016
SRR1780329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557407
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT408.3291525E-943.98177725
CTCGCTA250.002344062635.22336613
AAACTCG502.3660668E-630.78724327
CTATGCT554.9950922E-627.9708279
TCGTTTA407.055472E-427.4812132
GAAACTC609.772015E-625.65603826
TAACCGG450.001396254424.44748922
GGACAAA450.001402843524.42774233
GCTATAT551.6043875E-423.9728451
GACAAAT500.002566108222.0185634
GTATAGC500.002568788122.01460313
CGGCTTT500.002584916821.99088926
GATAGTC602.878136E-421.99088926
TATAGTC500.002595715521.9751075
TTAGAGT550.004456490720.01867314
TAGATCT1653.6379788E-1220.01687439
GACGAAA1101.2609053E-720.00249123
GGCTTTT550.004491382319.99171827
AATCGTC550.004491382319.99171828
TTTTGCC550.004496051519.98812931
Produced by FastQC (version 0.11.5)