FastQCFastQC Report
Wed 25 May 2016
SRR1780329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557407
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT201083.6074179190429976No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC51850.9302000154285827No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG49840.8941401884081112No Hit
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG38660.693568613239518RNA PCR Primer, Index 13 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC12790.2294553172098664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG5150.035.45363644
GTCTTCT5500.033.19749543
TCTTTAT1900.032.485631
CTTTATA1900.032.4215162
ATGCCGT27950.032.2694844
AGACTAT353.2170775E-431.4262036
CCGTCTT6100.029.93216741
CGTCTTC6350.029.10016642
AATATCG703.5861376E-828.28358336
TTTAGCC554.9576647E-627.9978879
TATGCCG32650.027.62425843
TAAATGT407.026761E-427.50039125
AAATGTC407.026761E-427.50039126
TCGTTTA407.030447E-427.49792732
CGTTTAT407.030447E-427.49792733
GCCGTCT6850.027.2972140
ATCGTAT655.925467E-727.0748839
ATATCGT655.925467E-727.0748837
TTTATAC2750.024.8003543
CTGTTTT450.001397153624.44479425