FastQCFastQC Report
Wed 25 May 2016
SRR1780328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158081
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTT200.00694100452.54
TAAGGTG200.00694100452.520
GTATACC305.863941E-446.6666641
GGCCGTA501.7038296E-435.00000446
TTGTACT400.00241314735.04
TGTCTGG601.20993245E-535.010
CGTTTGT601.20993245E-535.022
TTAGACG652.0926296E-532.30769339
GAGTAAC450.00429733631.11110911
AAGTGGT450.00429733631.11110927
TTTAGAC703.472026E-530.038
CGAAAGT500.00719186128.00000253
TCCGAGT500.00719186128.0000028
TGAAAGT500.00719186128.00000224
ATCTGTT500.00719186128.00000258
TTGTCTG755.5580746E-528.09
ATTACCA657.928835E-426.92307962
GACGTGG700.001221878325.08
TCTCGGT750.001826077423.33333443
GTTTAGA1052.0262383E-523.33333237
Produced by FastQC (version 0.11.5)