##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780328_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 158081 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07868118243179 34.0 32.0 34.0 21.0 34.0 2 31.03395094919693 34.0 33.0 34.0 21.0 34.0 3 30.93625419879682 34.0 33.0 34.0 21.0 34.0 4 30.799254812406296 34.0 32.0 34.0 21.0 34.0 5 30.79933072285727 34.0 32.0 34.0 21.0 34.0 6 33.89877973950063 38.0 34.0 38.0 21.0 38.0 7 34.18293786097001 38.0 34.0 38.0 23.0 38.0 8 34.214839227990716 38.0 35.0 38.0 23.0 38.0 9 34.4888569783845 38.0 36.0 38.0 24.0 38.0 10-11 34.28105211885047 38.0 35.5 38.0 23.0 38.0 12-13 34.36146026404185 38.0 35.5 38.0 23.0 38.0 14-15 34.35458087942257 38.0 35.5 38.0 23.0 38.0 16-17 34.417697256469786 38.0 36.0 38.0 23.0 38.0 18-19 34.42080958495961 38.0 36.0 38.0 23.0 38.0 20-21 34.430497656264826 38.0 36.0 38.0 23.5 38.0 22-23 34.43327471359619 38.0 36.0 38.0 23.0 38.0 24-25 34.4695377686123 38.0 36.0 38.0 23.5 38.0 26-27 34.46792150859369 38.0 36.0 38.0 23.0 38.0 28-29 34.585658618050246 38.0 36.0 38.0 24.0 38.0 30-31 34.60132147443399 38.0 36.0 38.0 24.0 38.0 32-33 34.650492469050675 38.0 36.0 38.0 24.5 38.0 34-35 34.606701627646586 38.0 36.0 38.0 24.5 38.0 36-37 34.54347454785838 38.0 36.0 38.0 24.0 38.0 38-39 34.57300055035077 38.0 36.0 38.0 24.0 38.0 40-41 34.6363288440736 38.0 36.0 38.0 24.5 38.0 42-43 34.67413541159279 38.0 36.0 38.0 25.0 38.0 44-45 34.727525762109295 38.0 36.0 38.0 25.0 38.0 46-47 34.66435561515932 38.0 36.0 38.0 24.5 38.0 48-49 34.638780119052896 38.0 36.0 38.0 24.5 38.0 50-51 34.576840353995735 38.0 36.0 38.0 24.0 38.0 52-53 34.62666923918751 38.0 36.0 38.0 24.0 38.0 54-55 34.61523522750994 38.0 36.0 38.0 24.0 38.0 56-57 34.5500186613192 38.0 36.0 38.0 24.0 38.0 58-59 34.62632764215813 38.0 36.0 38.0 24.0 38.0 60-61 34.63245741107407 38.0 36.0 38.0 24.5 38.0 62-63 34.530430602033135 38.0 36.0 38.0 24.0 38.0 64-65 34.49016643366376 38.0 36.0 38.0 24.0 38.0 66-67 34.345898621592724 38.0 36.0 38.0 23.5 38.0 68-69 34.28810862785534 38.0 36.0 38.0 23.0 38.0 70-71 34.23697345031977 38.0 35.5 38.0 23.0 38.0 72-73 34.22430273087848 38.0 36.0 38.0 23.0 38.0 74-75 34.22038385384708 38.0 36.0 38.0 23.0 38.0 76 32.60322239864373 37.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18507954714323915 1101 2 0.19762331288065837 1101 3 0.5402920483410938 1101 4 0.060623072281153156 1101 5 -0.31699328853127184 1101 6 -0.12342405225464859 1101 7 -0.041157080138454205 1101 8 -0.48565024883679087 1101 9 -0.8590666679318915 1101 10-11 -0.20028746889973803 1101 12-13 0.12308910708038212 1101 14-15 -0.07183203049110176 1101 16-17 -0.08305191481873919 1101 18-19 0.1246580394536636 1101 20-21 -0.26772842787841 1101 22-23 -0.5158853704036943 1101 24-25 -0.3147209615004911 1101 26-27 -0.9104529079344772 1101 28-29 -0.46028009469807785 1101 30-31 -0.6126973406064877 1101 32-33 -0.40069125381793924 1101 34-35 -0.5446578870239804 1101 36-37 -0.0032253891798532663 1101 38-39 -0.06792107692216831 1101 40-41 -0.09212557134167554 1101 42-43 -0.1161219013713648 1101 44-45 -0.2087342368091214 1101 46-47 -0.1833720773705778 1101 48-49 -0.23491649334152243 1101 50-51 -0.5321009933979113 1101 52-53 -0.16047224554212391 1101 54-55 -0.06120437844524673 1101 56-57 -0.26889687327716416 1101 58-59 0.05521845730618935 1101 60-61 -0.2154035754603072 1101 62-63 -0.10949289982260524 1101 64-65 -0.19196461720500935 1101 66-67 -0.3609432972502873 1101 68-69 -0.5644633865149586 1101 70-71 -0.401168773584601 1101 72-73 -0.34463246229627487 1101 74-75 -0.1716867748563473 1101 76 -1.2814395325877754 1102 1 0.5540601600262889 1102 2 0.6519697794222488 1102 3 0.6589286962460328 1102 4 0.8536812336319954 1102 5 0.018153678323013622 1102 6 0.05768914349269494 1102 7 0.13464029321989557 1102 8 0.4747625091369372 1102 9 0.8539414621494075 1102 10-11 0.8416762584298425 1102 12-13 0.7599871683686956 1102 14-15 0.6227270689460482 1102 16-17 0.7169480851812651 1102 18-19 0.37359487497586485 1102 20-21 0.5517524977000718 1102 22-23 0.4583485257814246 1102 24-25 0.5229901079178987 1102 26-27 0.406495184623374 1102 28-29 0.6212396051143045 1102 30-31 0.6992163554535509 1102 32-33 0.9090961132864948 1102 34-35 0.622008779642691 1102 36-37 0.7394262681059445 1102 38-39 0.5043741075681467 1102 40-41 0.532108324843442 1102 42-43 0.4116141836817917 1102 44-45 0.37597495643666434 1102 46-47 0.3526504367007206 1102 48-49 0.5691485473088846 1102 50-51 0.5739652980342385 1102 52-53 1.0488773479538054 1102 54-55 0.7393272037936498 1102 56-57 0.5982701060849394 1102 58-59 0.4963691765056879 1102 60-61 0.6924138104058599 1102 62-63 0.5323144797896475 1102 64-65 1.013132318379732 1102 66-67 0.5497508866146319 1102 68-69 0.8789387398140036 1102 70-71 0.9011764421752488 1102 72-73 1.0096827347018547 1102 74-75 0.5932287973762982 1102 76 1.085727446774328 1103 1 -0.5658664324435172 1103 2 0.18017247892496968 1103 3 -0.08930715504451925 1103 4 -0.5161506394440138 1103 5 -0.167839691319422 1103 6 -0.4688559666185981 1103 7 -0.010126460272879001 1103 8 -0.21320812785098298 1103 9 -0.12589514689629056 1103 10-11 -0.41139028194429983 1103 12-13 -0.28069481126663476 1103 14-15 -0.7705064143596658 1103 16-17 -0.22795482744009377 1103 18-19 -0.43742809447713427 1103 20-21 -0.6217675117718642 1103 22-23 -0.48292545006513166 1103 24-25 -0.43460520347210974 1103 26-27 -0.6935995347704278 1103 28-29 -0.47310683106850604 1103 30-31 -0.24540831894853454 1103 32-33 -0.32529294662352015 1103 34-35 -0.18138927860973553 1103 36-37 -0.28639068666554124 1103 38-39 -0.7836853288514618 1103 40-41 -0.35698118498370235 1103 42-43 -0.4528719636068743 1103 44-45 -0.5418559694454146 1103 46-47 -0.02961927677311138 1103 48-49 -0.4705151988431453 1103 50-51 -1.085654640398154 1103 52-53 -0.6095822779045932 1103 54-55 -0.8058894668196572 1103 56-57 -0.34779903907253384 1103 58-59 -0.43007529424969704 1103 60-61 -0.2920403690873883 1103 62-63 -0.7298688022373412 1103 64-65 -0.3702444280090944 1103 66-67 -0.7167125280195137 1103 68-69 -0.34389455405291613 1103 70-71 -0.27906272429409285 1103 72-73 -0.48384082674735396 1103 74-75 -0.5244126897181829 1103 76 -0.4365552906161554 1104 1 -0.570021635826798 1104 2 -0.6050188536959347 1104 3 -0.3523639295607879 1104 4 0.14723809660109666 1104 5 -1.2250518965898785 1104 6 -0.75135295395404 1104 7 -0.6674046031072862 1104 8 -0.9380552209138955 1104 9 -1.1176927413120623 1104 10-11 -0.6260966324138266 1104 12-13 -0.7920463039566599 1104 14-15 -0.48173114776727033 1104 16-17 -0.5150191279334848 1104 18-19 -0.5302750056433112 1104 20-21 -1.2630415846669791 1104 22-23 -0.6911630981404429 1104 24-25 -0.6637751859645462 1104 26-27 -0.7639537786484638 1104 28-29 -0.8252503703240919 1104 30-31 -0.5614993582609671 1104 32-33 -0.5840666216195416 1104 34-35 -0.7228389955329533 1104 36-37 -0.6339015436265001 1104 38-39 -0.6362210829270865 1104 40-41 -0.7807522452142521 1104 42-43 -0.40906611562706274 1104 44-45 -0.4278749321793214 1104 46-47 -0.5043953164266028 1104 48-49 -0.48800002183489255 1104 50-51 -0.6970862813340162 1104 52-53 -0.8760069840276827 1104 54-55 -0.7989603383323498 1104 56-57 -0.42487777761871826 1104 58-59 -0.3880770450057085 1104 60-61 -0.32294576005167386 1104 62-63 -0.5734603531343225 1104 64-65 -0.5374188897084551 1104 66-67 -0.2913612399633081 1104 68-69 -0.5007151129306138 1104 70-71 -0.5372253399690408 1104 72-73 -0.36811471774574045 1104 74-75 -0.5788566541573772 1104 76 -0.19772488657805098 1105 1 0.12147126787230889 1105 2 -0.0695994242215967 1105 3 0.1910180291875534 1105 4 0.3586768257565929 1105 5 0.2155677247526313 1105 6 0.14240850875863487 1105 7 0.33076136286432956 1105 8 -0.38212846941139844 1105 9 0.15820240837332733 1105 10-11 0.3015352064169079 1105 12-13 -0.6921873834973056 1105 14-15 0.23229215857284657 1105 16-17 0.028393868313798976 1105 18-19 0.302260758020239 1105 20-21 -0.1602369233989549 1105 22-23 0.16795769416226136 1105 24-25 -0.054294640833205676 1105 26-27 0.2490311822724962 1105 28-29 0.13828339000998113 1105 30-31 0.36014788645560714 1105 32-33 0.2761545911001946 1105 34-35 0.5346593820523324 1105 36-37 -0.03308328229076096 1105 38-39 0.4888290003098419 1105 40-41 0.30015122816404016 1105 42-43 0.19252808318223202 1105 44-45 0.19437048978958416 1105 46-47 0.35147500325963676 1105 48-49 0.19359756288337593 1105 50-51 0.5940652098767245 1105 52-53 0.17125466208839413 1105 54-55 0.415054503235325 1105 56-57 0.05441174581459052 1105 58-59 0.14700097198026896 1105 60-61 0.22858777455514456 1105 62-63 0.25267886415638685 1105 64-65 0.11463099601710525 1105 66-67 0.2281669900527703 1105 68-69 0.40479827551779124 1105 70-71 0.1815467037091878 1105 72-73 0.15226281110501816 1105 74-75 0.2723354679610779 1105 76 0.33644739975698457 1106 1 -0.26818878077297725 1106 2 0.10961320092688354 1106 3 0.7406531895473023 1106 4 0.5931980090814406 1106 5 0.06736929723976459 1106 6 0.558880028640985 1106 7 -0.3164189075129542 1106 8 -0.1321652361968546 1106 9 0.11558121939205535 1106 10-11 -0.19730805394849682 1106 12-13 0.2374805841479244 1106 14-15 -0.0077836375694673166 1106 16-17 0.48596216968830674 1106 18-19 0.08807443526442427 1106 20-21 0.50616106291249 1106 22-23 0.3563789849592567 1106 24-25 0.4696235672102418 1106 26-27 0.34801585335117835 1106 28-29 0.3290662397186779 1106 30-31 -0.14229286245552686 1106 32-33 0.40898733050853053 1106 34-35 0.2288162913797791 1106 36-37 0.11831668377067217 1106 38-39 0.21694710653756744 1106 40-41 0.10150028636869024 1106 42-43 0.3157307530165028 1106 44-45 0.31102018717065505 1106 46-47 0.228328897710675 1106 48-49 0.19301777895894645 1106 50-51 0.05845516405965867 1106 52-53 0.06816625211045846 1106 54-55 0.34253343303904416 1106 56-57 0.1540355933172748 1106 58-59 0.2462260412715196 1106 60-61 0.25518147329375296 1106 62-63 0.34063349959956923 1106 64-65 0.546912233643674 1106 66-67 0.15124521845935845 1106 68-69 0.4259844285807475 1106 70-71 0.3305935955017105 1106 72-73 0.22293705738591996 1106 74-75 0.37668808971569945 1106 76 0.11754927203482879 1107 1 0.5801344921981837 1107 2 -0.047797932540586174 1107 3 0.17710523515427923 1107 4 0.1492677609258415 1107 5 0.17256715102916687 1107 6 -0.7486987775293699 1107 7 -0.14683473581610684 1107 8 0.18852557533902825 1107 9 0.17426666540143998 1107 10-11 0.06711179849953197 1107 12-13 0.421166030157309 1107 14-15 0.08686760320853182 1107 16-17 0.6288528470860228 1107 18-19 0.5481012628968855 1107 20-21 0.4852019750519929 1107 22-23 0.33567140615308233 1107 24-25 0.5452607234811992 1107 26-27 0.3572210847395141 1107 28-29 0.6108737514557632 1107 30-31 0.10058104767190201 1107 32-33 0.12018786706117623 1107 34-35 0.1267706844045975 1107 36-37 0.32113358517911905 1107 38-39 -0.1251555091565919 1107 40-41 0.22847882426271582 1107 42-43 0.22032498507551423 1107 44-45 0.006375653300764839 1107 46-47 0.23961327061477533 1107 48-49 0.1388930304679974 1107 50-51 0.4077341714372622 1107 52-53 0.23952775445787466 1107 54-55 0.11755020030933139 1107 56-57 0.23659763221733954 1107 58-59 0.023808200895935272 1107 60-61 0.4924718824151526 1107 62-63 0.27493933460962694 1107 64-65 0.30895113371074245 1107 66-67 0.14043032912334752 1107 68-69 0.153851631800066 1107 70-71 0.3719081494923131 1107 72-73 0.5259719333081208 1107 74-75 0.11860626543669639 1107 76 -0.07118312233136947 1108 1 -0.04670166599390768 1108 2 -0.03343150800467143 1108 3 -0.12051381246476822 1108 4 0.17557930249162013 1108 5 0.6393548830711353 1108 6 0.9392753951905632 1108 7 -0.051717705953315374 1108 8 -0.1527577176391759 1108 9 -0.30111670828701875 1108 10-11 0.436361193172651 1108 12-13 0.04838556203785771 1108 14-15 0.6384212755249123 1108 16-17 0.5221538963192813 1108 18-19 0.4317977923561287 1108 20-21 9.404738413394398E-4 1108 22-23 0.3888392834735015 1108 24-25 0.11368283808975121 1108 26-27 0.25490066504621467 1108 28-29 0.38018367881654314 1108 30-31 0.3420055835879623 1108 32-33 0.04323064349378569 1108 34-35 0.19283202419475032 1108 36-37 0.05914811302415757 1108 38-39 0.14657572571023536 1108 40-41 0.2646571939156601 1108 42-43 0.25967123204888054 1108 44-45 -0.060391901178647345 1108 46-47 -0.05895412244414189 1108 48-49 -0.17543303894281337 1108 50-51 0.2655025330191805 1108 52-53 0.0781072540543235 1108 54-55 0.3193661809388715 1108 56-57 0.17473322543768433 1108 58-59 -0.17504167803868853 1108 60-61 0.09218939653944602 1108 62-63 -0.09608568556793529 1108 64-65 0.27117873661631364 1108 66-67 0.32898965115724366 1108 68-69 -0.08585781129920633 1108 70-71 0.30580645457706623 1108 72-73 -0.12106196499696864 1108 74-75 0.42808971983539834 1108 76 0.006379428112538221 1109 1 0.25528539763077873 1109 2 0.18238315397852745 1109 3 0.02938558391350199 1109 4 0.4406158494570285 1109 5 -0.18145699384004743 1109 6 0.8053589018804175 1109 7 0.7540036276983102 1109 8 0.13778781544634455 1109 9 0.6202760550728073 1109 10-11 0.24400650004211144 1109 12-13 0.03325523203524483 1109 14-15 0.3912773952943809 1109 16-17 0.40262883635497815 1109 18-19 0.466008707564896 1109 20-21 0.04371974835849812 1109 22-23 0.2167076234569052 1109 24-25 -0.01394450790713364 1109 26-27 0.35271068903193026 1109 28-29 0.0579394791553014 1109 30-31 0.2849371844928257 1109 32-33 0.06156949013293911 1109 34-35 0.1630885918492666 1109 36-37 0.10305846780822492 1109 38-39 0.3683555572417916 1109 40-41 0.021688079795914916 1109 42-43 0.15845375771133519 1109 44-45 0.44364929515187157 1109 46-47 0.27058241883744216 1109 48-49 0.14841683999181043 1109 50-51 0.3413190138249149 1109 52-53 0.06464512534989098 1109 54-55 0.28971653163058875 1109 56-57 0.2584956872139941 1109 58-59 0.6241603135719913 1109 60-61 0.1953223183641839 1109 62-63 0.2596475841066095 1109 64-65 0.46475260923052275 1109 66-67 0.3425597724499738 1109 68-69 0.38607832529436337 1109 70-71 0.487870590806871 1109 72-73 0.05744409963944008 1109 74-75 0.489364146053731 1109 76 -0.0444225589510836 1110 1 0.1355782926300506 1110 2 0.3674538006109067 1110 3 0.42091134948545417 1110 4 0.21604807687083394 1110 5 -0.037530587350193656 1110 6 0.09416973025560083 1110 7 -0.13480495970518547 1110 8 -0.0037519561986414374 1110 9 -0.22271026379505798 1110 10-11 -0.2625847579273355 1110 12-13 -0.16644335727307435 1110 14-15 -0.25091225813934415 1110 16-17 -0.35667959978293595 1110 18-19 -0.25620604475170694 1110 20-21 -0.08179427452182608 1110 22-23 -0.04790911436061407 1110 24-25 -0.08114284510614311 1110 26-27 -0.015522977085694833 1110 28-29 -0.21209760892609353 1110 30-31 -0.10050714416808404 1110 32-33 0.2472648076226065 1110 34-35 0.12260543835390791 1110 36-37 0.1060977856564449 1110 38-39 -0.20758817195918766 1110 40-41 -0.21698941076399336 1110 42-43 -0.056780367805494336 1110 44-45 0.2109187832018975 1110 46-47 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-0.31058181944704444 2116 10-11 0.275079506467236 2116 12-13 0.03490476889604821 2116 14-15 0.3279317192726481 2116 16-17 -0.09398557575239863 2116 18-19 -0.0048763900807671234 2116 20-21 0.25441924426925766 2116 22-23 0.06328050876218327 2116 24-25 0.21351566673613576 2116 26-27 0.20897316149159195 2116 28-29 -0.11203779645578749 2116 30-31 0.3098503819412386 2116 32-33 0.3049252017985111 2116 34-35 0.2270456346795484 2116 36-37 0.22825889230442442 2116 38-39 0.17580096679987633 2116 40-41 -0.37708745630356333 2116 42-43 -0.13567712092658724 2116 44-45 0.1013300232551444 2116 46-47 0.039364645366141815 2116 48-49 0.023723965298941607 2116 50-51 0.08722112592420928 2116 52-53 0.42093643586655105 2116 54-55 0.1875923203514489 2116 56-57 -0.2127354671157562 2116 58-59 0.13345193553966794 2116 60-61 0.06192900187227224 2116 62-63 0.14786550753580485 2116 64-65 -0.22660520184559374 2116 66-67 0.06246437615739353 2116 68-69 -0.1887247107762846 2116 70-71 0.10483198241615099 2116 72-73 -0.06157632924013967 2116 74-75 0.1281242249546537 2116 76 0.17115922001097772 2117 1 -0.21423252290053796 2117 2 -0.46510339130945866 2117 3 -0.321559108632016 2117 4 -0.3926602297826385 2117 5 -0.40135851679893975 2117 6 -0.01304256382437785 2117 7 -0.15316458482888606 2117 8 -0.32767651525330166 2117 9 -0.14605853785059253 2117 10-11 -0.09734813181405855 2117 12-13 0.22584082690527651 2117 14-15 -0.002882687151519292 2117 16-17 -0.5476860611601921 2117 18-19 -0.05323439331682067 2117 20-21 -0.49336945351943484 2117 22-23 0.012007062366784282 2117 24-25 -0.22457086769185963 2117 26-27 0.15405616023312518 2117 28-29 -0.3507116143978948 2117 30-31 -0.2373690104001085 2117 32-33 -0.5665136428110671 2117 34-35 0.15005756013049876 2117 36-37 0.140645780301071 2117 38-39 -0.13099174609039466 2117 40-41 -0.07764777271753331 2117 42-43 -0.14326386509869593 2117 44-45 -0.21670797039260492 2117 46-47 0.028260192798278183 2117 48-49 -0.0028026722033160922 2117 50-51 -0.20652250684381102 2117 52-53 -0.04258606668033593 2117 54-55 -0.005794747425866831 2117 56-57 -0.31392501586628185 2117 58-59 -0.07558204300649862 2117 60-61 -0.7575861895941429 2117 62-63 -0.31646600801522595 2117 64-65 -0.7295506084495358 2117 66-67 -0.17951740606829958 2117 68-69 -0.6113051626250225 2117 70-71 -0.13296989928817027 2117 72-73 -1.529341655542055 2117 74-75 -0.5457955928676057 2117 76 -0.03866279712811149 2118 1 -0.37677942721573743 2118 2 -0.29788950763215993 2118 3 -0.14352205422300912 2118 4 -1.1369730815649994 2118 5 -0.25080739109537475 2118 6 -0.28079584712644134 2118 7 -0.38442631090768487 2118 8 -0.3962271719137149 2118 9 -0.3505730781883045 2118 10-11 -1.3717618278740957 2118 12-13 -0.5904525595862751 2118 14-15 -0.6093320304911032 2118 16-17 -0.7551672994341203 2118 18-19 -0.8461752938796678 2118 20-21 -0.6777444535194377 2118 22-23 -0.7061097293780492 2118 24-25 -0.20077865380817883 2118 26-27 -0.45941124126780863 2118 28-29 -0.4631647100826939 2118 30-31 -0.2143800329141854 2118 32-33 -0.5216848435615304 2118 34-35 -0.37558737420346233 2118 36-37 -0.6113183378977993 2118 38-39 -0.6615909487170342 2118 40-41 -0.5830710841621922 2118 42-43 -0.36844561932009157 2118 44-45 -0.9604169291168105 2118 46-47 -0.8540451542936509 2118 48-49 -0.88042931385435 2118 50-51 -0.4760112498081597 2118 52-53 -0.6575075019523808 2118 54-55 -0.6113646348555051 2118 56-57 -0.4362045655848519 2118 58-59 -0.37162449141175813 2118 60-61 -0.8106760113577351 2118 62-63 -0.5291243100790197 2118 64-65 -0.6680062838716765 2118 66-67 -0.7539721434041269 2118 68-69 -0.7302486429252113 2118 70-71 -0.581457235123068 2118 72-73 -0.7054497699885829 2118 74-75 -0.44139831331788315 2118 76 -0.4708626095108528 2119 1 0.8931918911969063 2119 2 0.9030461703817565 2119 3 -0.14880581386190528 2119 4 0.4861637907314069 2119 5 1.3208789451844822 2119 6 0.7250932820817582 2119 7 1.252786873218703 2119 8 0.7136914852237695 2119 9 0.6983090451895464 2119 10-11 1.0302623398896813 2119 12-13 0.8507707571336738 2119 14-15 0.8415683832760621 2119 16-17 0.8444541976507054 2119 18-19 0.7545746591013298 2119 20-21 0.37081147557591976 2119 22-23 0.4972297947897104 2119 24-25 0.6721701425055286 2119 26-27 0.8083766325332036 2119 28-29 0.5361182502332724 2119 30-31 0.5570224261792944 2119 32-33 0.5509888916274832 2119 34-35 0.447803400669585 2119 36-37 0.7267570570617607 2119 38-39 0.5351570999946418 2119 40-41 0.40411917820380694 2119 42-43 0.34091527498120655 2119 44-45 0.48617830785892124 2119 46-47 0.38687430216675267 2119 48-49 0.35685204781577085 2119 50-51 0.5852897658021448 2119 52-53 0.6164633697797939 2119 54-55 0.35378841197541533 2119 56-57 0.3773416070688995 2119 58-59 0.48805025468566754 2119 60-61 0.4070777736714106 2119 62-63 0.3844165729377167 2119 64-65 0.41649566741664756 2119 66-67 0.25975446464552476 2119 68-69 0.09814083892545966 2119 70-71 0.009071963673250139 2119 72-73 0.57365792702101 2119 74-75 0.3871998153260847 2119 76 0.27054466902967533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 10.0 13 213.0 14 864.0 15 1448.0 16 1839.0 17 2201.0 18 2214.0 19 2061.0 20 1577.0 21 1172.0 22 980.0 23 890.0 24 902.0 25 981.0 26 1125.0 27 1336.0 28 1668.0 29 2030.0 30 2614.0 31 3297.0 32 4201.0 33 5495.0 34 7675.0 35 11802.0 36 22065.0 37 77420.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.27124701893333 21.07716929928328 10.691354432221456 26.960229249561934 2 21.731896938911063 20.42054389838121 30.01435972697541 27.833199435732315 3 20.491393652621127 21.694574300516823 27.775634010412382 30.03839803644967 4 15.472447669232862 18.536699540109183 33.237390957800116 32.75346183285784 5 16.459916118951675 31.752709054219043 32.48271455772673 19.30466026910255 6 28.992731574319496 32.719934717012165 18.297581619549472 19.98975208911887 7 28.479703443171537 26.756536206122178 21.95077207254509 22.812988278161196 8 25.237062012512574 30.509675419563386 19.643094363016427 24.610168204907612 9 24.938480905358645 17.070362662179516 20.54832649084963 37.44282994161221 10-11 26.7116225226308 22.87055370348113 24.905270083058685 25.512553690829385 12-13 25.71118603753772 20.93546979080345 24.701260746073217 28.652083425585616 14-15 24.0231273840626 22.018458891327864 23.062860179275184 30.89555354533435 16-17 24.974222076024315 24.333411352407943 23.808680360068575 26.883686211499167 18-19 24.38686496163359 23.86751095957136 24.59561870180477 27.150005376990276 20-21 24.162929131268147 24.12529019932819 24.94290901499864 26.768871654405018 22-23 25.54449933894649 23.79697749887716 24.25813348852803 26.400389673648316 24-25 24.591268372759448 24.557741150869337 23.8520880184463 26.998902457924917 26-27 23.792865682782878 24.712331020173202 25.05867245272993 26.436130844313993 28-29 24.545644321581975 24.03609541943687 25.838336042914705 25.579924216066445 30-31 24.527693168986687 23.569636988749405 25.53667277115141 26.365997071112503 32-33 23.561338807320297 24.672161739867537 25.50812558118939 26.258373871622776 34-35 24.1322486573339 24.319178142850816 25.76147671130623 25.787096488509054 36-37 24.51812678310486 23.507568904548933 25.568221354875032 26.40608295747117 38-39 23.397256461106842 23.76289295643675 26.85530473398047 25.98454584847594 40-41 23.48036765961754 22.90502969996394 27.240465331064456 26.37413730935407 42-43 22.44419000385878 22.6425060570214 27.637730024481122 27.275573914638695 44-45 21.773083966713163 22.363605884343738 28.730615098003863 27.132695050939237 46-47 21.581024917605532 22.91515109342679 28.974386548668086 26.529437440299592 48-49 21.185341691917433 23.319690538394873 28.894047988056755 26.60091978163094 50-51 20.747275131103674 24.367571055345046 27.947065112189325 26.938088701361963 52-53 20.096343014024455 24.48206931889348 28.152655916903356 27.268931750178705 54-55 19.91131128978182 24.297353888196557 27.854707396840862 27.93662742518076 56-57 20.159601723167235 24.392874539002158 27.642158134121114 27.805365603709493 58-59 20.616013309632404 24.178743808553843 27.050689203636107 28.154553678177642 60-61 20.77922077922078 23.221006952132132 27.526078402844114 28.473693865802975 62-63 21.221082862583106 23.135923988335094 26.92417178535055 28.71882136373125 64-65 21.91503090187942 23.485744649894674 25.630214889834956 28.96900955839095 66-67 21.930212992073685 23.747003118654362 24.77527343577027 29.547510453501687 68-69 22.037436504070698 24.013954871236898 24.85940751893017 29.089201105762235 70-71 23.22891429077498 23.834616430817114 25.11244235550129 27.824026922906608 72-73 24.372315458530753 23.4180578311119 24.812279780618795 27.397346929738553 74-75 23.93583036544556 24.65950999803898 24.648756017484708 26.755903619030754 76 23.76060374112006 24.580436611610505 24.63610427565615 27.022855371613286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.5 15 2.5 16 5.5 17 7.0 18 11.5 19 17.5 20 27.0 21 35.0 22 48.5 23 87.0 24 135.5 25 159.0 26 232.0 27 401.0 28 536.0 29 575.0 30 741.0 31 1041.0 32 1473.0 33 1771.0 34 2059.0 35 2680.5 36 3281.0 37 3548.0 38 4155.5 39 5332.5 40 6643.5 41 7631.5 42 7878.0 43 8072.0 44 8703.0 45 9229.5 46 9319.0 47 9428.5 48 9193.0 49 8482.0 50 8116.0 51 7581.0 52 6391.0 53 5278.0 54 4820.0 55 4539.0 56 3833.5 57 3201.5 58 2994.0 59 2819.0 60 2666.0 61 2674.0 62 2660.0 63 2816.5 64 2917.5 65 2851.5 66 2888.5 67 2936.0 68 2951.0 69 2776.5 70 2548.5 71 2510.0 72 2393.5 73 2105.0 74 1725.0 75 1517.0 76 1324.5 77 955.0 78 598.0 79 418.0 80 299.5 81 129.5 82 54.5 83 31.0 84 21.5 85 10.5 86 9.0 87 9.0 88 6.5 89 4.0 90 4.5 91 7.0 92 9.0 93 6.0 94 3.5 95 4.5 96 5.0 97 3.5 98 3.5 99 15.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.162935457139062E-4 26-27 0.0 28-29 0.0 30-31 3.162935457139062E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.162935457139062E-4 40-41 0.0 42-43 0.0 44-45 3.162935457139062E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 158081.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.04366116105035 #Duplication Level Percentage of deduplicated Percentage of total 1 74.41257247482453 43.19178142850817 2 13.218754086926197 15.345297663855872 3 4.699420201403723 8.18314661471018 4 2.2276472383277386 5.172032059513794 5 1.3208945464056847 3.8334777740525428 6 0.9459871833994506 3.2945135721560463 7 0.672435590043158 2.7321436478767214 8 0.5176773181045381 2.403830947425687 9 0.36836828109333447 1.924329932123405 >10 1.6151532324861588 13.886551831023336 >50 0.0010898469854832382 0.03289452875424624 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0075910450971337475 0.0 2 0.0 0.0 0.0 0.011386567645700622 0.0 3 0.0 0.0 0.0 0.011386567645700622 0.0 4 0.0 0.0 0.0 0.04491368349137467 0.0 5 0.0 0.0 0.0 0.05060696731422499 0.0 6 0.0 0.0 0.0 0.051239554405652796 0.0 7 0.0 0.0 0.0 0.05313731567993624 0.0 8 0.0 0.0 0.0 0.05376990277136405 0.0 9 0.0 0.0 0.0 0.05946318659421436 0.0 10 0.0 0.0 0.0 0.061360947868497794 0.0 11 0.0 0.0 0.0 0.06389129623420904 0.0 12 0.0 0.0 0.0 0.06389129623420904 0.0 13 0.0 0.0 0.0 0.06389129623420904 0.0 14 0.0 0.0 0.0 0.06515647041706467 0.0 15 0.0 0.0 0.0 0.06515647041706467 0.0 16 0.0 0.0 0.0 0.06515647041706467 0.0 17 0.0 0.0 0.0 0.0664216445999203 0.0 18 0.0 0.0 0.0 0.06705423169134811 0.0 19 0.0 0.0 0.0 0.06895199296563154 0.0 20 0.0 0.0 0.0 0.07464527678848186 0.0 21 0.0 0.0 0.0 0.07527786387990967 0.0 22 0.0 0.0 0.0 0.0771756251541931 0.0 23 0.0 0.0 0.0 0.08097114770275998 0.0 24 0.0 0.0 0.0 0.08476667025132685 0.0 25 0.0 0.0 0.0 0.09362288953131623 0.0 26 0.0 0.0 0.0 0.12145672155413996 0.0 27 0.0 0.0 0.0 0.2365875721940018 0.0 28 0.0 0.0 0.0 0.3295778746338902 0.0 29 0.0 0.0 0.0 0.44470872527375205 0.0 30 0.0 0.0 0.0 0.535801266439357 0.0 31 0.0 0.0 0.0 0.6642164459992029 0.0 32 0.0 0.0 0.0 0.8236283930390117 0.0 33 0.0 0.0 0.0 0.9887336239016706 0.0 34 0.0 0.0 0.0 1.20001771243856 0.0 35 0.0 0.0 0.0 1.5175764323353218 0.0 36 0.0 0.0 0.0 2.0173202345632935 0.0 37 0.0 0.0 0.0 2.7618752411738288 0.0 38 0.0 0.0 0.0 3.652557865904188 0.0 39 0.0 0.0 0.0 4.53122133589742 0.0 40 0.0 0.0 0.0 5.549686553096198 0.0 41 0.0 0.0 0.0 6.6307778923463285 0.0 42 0.0 0.0 0.0 7.864322720630563 0.0 43 0.0 0.0 0.0 9.092806852183374 0.0 44 0.0 0.0 0.0 10.264990732599111 0.0 45 0.0 0.0 0.0 11.38403729733491 0.0 46 0.0 0.0 0.0 12.480943313870737 0.0 47 0.0 0.0 0.0 13.493715247246664 0.0 48 0.0 0.0 0.0 14.438167774748388 0.0 49 0.0 0.0 0.0 15.315566070558765 0.0 50 0.0 0.0 0.0 16.136031528140638 0.0 51 0.0 0.0 0.0 17.035570372150985 0.0 52 0.0 0.0 0.0 17.926252996881345 0.0 53 0.0 0.0 0.0 18.87892915657163 0.0 54 0.0 0.0 0.0 19.76138814911343 0.0 55 0.0 0.0 0.0 20.636256096558093 0.0 56 0.0 0.0 0.0 21.49341160544278 0.0 57 0.0 0.0 0.0 22.20507208329907 0.0 58 0.0 0.0 0.0 22.9154673869725 0.0 59 0.0 0.0 0.0 23.662552741948748 0.0 60 0.0 0.0 0.0 24.34701197487364 0.0 61 0.0 0.0 0.0 25.001739614501428 0.0 62 0.0 0.0 0.0 25.675444866872045 0.0 63 0.0 0.0 0.0 26.426958331488287 0.0 64 0.0 0.0 0.0 27.132925525521728 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTT 20 0.006941004 52.5 4 TAAGGTG 20 0.006941004 52.5 20 GTATACC 30 5.863941E-4 46.666664 1 GGCCGTA 50 1.7038296E-4 35.000004 46 TTGTACT 40 0.002413147 35.0 4 TGTCTGG 60 1.20993245E-5 35.0 10 CGTTTGT 60 1.20993245E-5 35.0 22 TTAGACG 65 2.0926296E-5 32.307693 39 GAGTAAC 45 0.004297336 31.111109 11 AAGTGGT 45 0.004297336 31.111109 27 TTTAGAC 70 3.472026E-5 30.0 38 CGAAAGT 50 0.007191861 28.000002 53 TCCGAGT 50 0.007191861 28.000002 8 TGAAAGT 50 0.007191861 28.000002 24 ATCTGTT 50 0.007191861 28.000002 58 TTGTCTG 75 5.5580746E-5 28.0 9 ATTACCA 65 7.928835E-4 26.923079 62 GACGTGG 70 0.0012218783 25.0 8 TCTCGGT 75 0.0018260774 23.333334 43 GTTTAGA 105 2.0262383E-5 23.333332 37 >>END_MODULE