FastQCFastQC Report
Wed 25 May 2016
SRR1780328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158081
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT64814.099796939543652No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC19611.2405032862899399No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG16821.0640114877815803No Hit
CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG12980.8210980446733005TruSeq Adapter, Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4500.28466419114251557No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2470.15624901158266966TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT1940.12272189573699559No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG150.002221346170.017
AAATGGA150.002221346170.014
ACGTAGC252.3847105E-456.00000433
TAGCTCG252.3847105E-456.00000436
CTTTATA1000.052.5000042
TAAACCG200.00694100452.512
ATAGACC200.00694100452.514
AGTCTTG200.00694100452.55
CAGTCTT200.00694100452.54
CGACTAA200.00694100452.537
GCGCTTT200.00694100452.529
GATAGAC200.00694100452.513
CGAAGAT200.00694100452.517
CTTTTAG305.863941E-446.6666641
TCTTTAT1150.045.6521721
TTTATAC1600.037.18753
CGTCTTC15850.035.7728748
CCGTCTT15850.035.7728747
TCGTATG15650.035.5591140
CGTATGC15750.035.55555741
Produced by FastQC (version 0.11.5)