##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780328_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 158081 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.773584428236155 34.0 34.0 34.0 34.0 34.0 2 33.750317875013444 34.0 34.0 34.0 34.0 34.0 3 33.75818093255989 34.0 34.0 34.0 34.0 34.0 4 33.774836950677184 34.0 34.0 34.0 34.0 34.0 5 33.76704347771079 34.0 34.0 34.0 34.0 34.0 6 37.520840581727086 38.0 38.0 38.0 37.0 38.0 7 37.51125056142104 38.0 38.0 38.0 37.0 38.0 8 37.447669232861635 38.0 38.0 38.0 37.0 38.0 9 37.456689924785394 38.0 38.0 38.0 37.0 38.0 10-11 37.40147139757466 38.0 38.0 38.0 37.0 38.0 12-13 37.38933837716108 38.0 38.0 38.0 37.0 38.0 14-15 37.307158988113684 38.0 38.0 38.0 36.0 38.0 16-17 37.35576065434809 38.0 38.0 38.0 36.5 38.0 18-19 37.332051290161374 38.0 38.0 38.0 36.5 38.0 20-21 37.336745086379764 38.0 38.0 38.0 36.5 38.0 22-23 37.31269412516368 38.0 38.0 38.0 36.0 38.0 24-25 37.328572061158525 38.0 38.0 38.0 36.5 38.0 26-27 37.24506423921913 38.0 38.0 38.0 36.0 38.0 28-29 37.21203054130477 38.0 38.0 38.0 36.0 38.0 30-31 37.18336169432126 38.0 38.0 38.0 36.0 38.0 32-33 37.175422726323845 38.0 38.0 38.0 36.0 38.0 34-35 37.182608915682465 38.0 38.0 38.0 36.0 38.0 36-37 37.156875272803184 38.0 38.0 38.0 36.0 38.0 38-39 36.79499750128099 38.0 38.0 38.0 35.0 38.0 40-41 36.97854580879422 38.0 38.0 38.0 35.5 38.0 42-43 37.10397834021799 38.0 38.0 38.0 36.0 38.0 44-45 37.16660129933389 38.0 38.0 38.0 36.0 38.0 46-47 37.11498219267338 38.0 38.0 38.0 36.0 38.0 48-49 37.13837842624984 38.0 38.0 38.0 36.0 38.0 50-51 37.02387383683049 38.0 38.0 38.0 36.0 38.0 52-53 37.03315072652627 38.0 38.0 38.0 36.0 38.0 54-55 37.00454197531646 38.0 38.0 38.0 36.0 38.0 56-57 37.04720997463326 38.0 38.0 38.0 36.0 38.0 58-59 36.96410068256147 38.0 38.0 38.0 36.0 38.0 60-61 36.97228319658909 38.0 38.0 38.0 36.0 38.0 62-63 36.953470056490026 38.0 38.0 38.0 35.5 38.0 64-65 36.93868965909881 38.0 38.0 38.0 35.5 38.0 66-67 36.728544227326495 38.0 37.5 38.0 34.5 38.0 68-69 36.67348700982408 38.0 38.0 38.0 34.5 38.0 70-71 36.22133273448422 38.0 37.0 38.0 32.5 38.0 72-73 35.51174397935236 38.0 37.0 38.0 29.0 38.0 74-75 35.274808484258074 38.0 37.0 38.0 26.5 38.0 76 34.518335536845036 38.0 36.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.003907529890177841 1101 2 -0.0028866853083613364 1101 3 0.03990053596663756 1101 4 0.08365894230490056 1101 5 0.044556679295546076 1101 6 -0.07517521096598045 1101 7 0.01812857912199206 1101 8 0.07939193031803171 1101 9 0.04765784753887914 1101 10-11 0.07486773757575804 1101 12-13 0.1053100890735692 1101 14-15 0.15776670528545367 1101 16-17 0.17602568255393436 1101 18-19 0.20972057465071714 1101 20-21 0.15001301469179396 1101 22-23 -0.034637508561012 1101 24-25 0.06863673450382635 1101 26-27 0.10287976991937597 1101 28-29 -0.02345274349492854 1101 30-31 0.006986889559506437 1101 32-33 -0.06621593908122492 1101 34-35 0.0841404444093996 1101 36-37 0.19547684411229937 1101 38-39 -0.044152208782179514 1101 40-41 0.05186089182494413 1101 42-43 0.09752101471192987 1101 44-45 0.14876439968264776 1101 46-47 0.17751708725586468 1101 48-49 0.11367261471255574 1101 50-51 0.068611127850005 1101 52-53 0.04022606226119052 1101 54-55 0.16418073513377607 1101 56-57 0.07292593435170858 1101 58-59 0.1269707763736534 1101 60-61 0.1582849845049381 1101 62-63 0.1307016966852288 1101 64-65 -0.008217683294617473 1101 66-67 0.017004582100241805 1101 68-69 -0.22210344626869016 1101 70-71 -0.35315394329339256 1101 72-73 -0.3872076063337744 1101 74-75 -0.15514231524910826 1101 76 -0.9080351545663135 1102 1 0.10784749236673008 1102 2 0.03169742976355394 1102 3 -0.02301378548426669 1102 4 0.0033587546876390206 1102 5 0.03867800512418995 1102 6 0.15340515176072955 1102 7 0.030261161986281593 1102 8 0.07370087340746068 1102 9 0.13765159363018142 1102 10-11 0.09756942613110908 1102 12-13 0.13489107719114202 1102 14-15 0.19256970716162414 1102 16-17 0.13950285578720667 1102 18-19 0.13013958653314006 1102 20-21 0.09813684208391749 1102 22-23 0.13109107180171264 1102 24-25 0.1487493048602957 1102 26-27 0.2436239850538371 1102 28-29 0.17898628089531599 1102 30-31 0.29310321226119385 1102 32-33 0.2833150177668031 1102 34-35 0.3127208071361025 1102 36-37 0.2099859122799046 1102 38-39 0.2615388481283887 1102 40-41 0.16077271171238294 1102 42-43 0.35224271577509825 1102 44-45 0.2290958568433723 1102 46-47 0.1749529846917568 1102 48-49 0.16670576042862706 1102 50-51 0.17067491771867793 1102 52-53 0.31283394218614546 1102 54-55 0.27334271136892596 1102 56-57 0.28061824204401375 1102 58-59 0.3003916644286875 1102 60-61 0.17163707956434848 1102 62-63 0.24743090243882904 1102 64-65 0.21833015910688403 1102 66-67 0.26203272468935523 1102 68-69 0.08152382077321363 1102 70-71 0.3491287333795299 1102 72-73 0.6576954580814913 1102 74-75 0.64376325072962 1102 76 0.8340536509371219 1103 1 -0.07114848205903712 1103 2 0.09589349890871546 1103 3 -0.0022702382429713452 1103 4 0.07731092139377438 1103 5 0.0856943442196183 1103 6 -0.046559328466599936 1103 7 -0.0210175498299634 1103 8 0.07340487530184703 1103 9 -0.04727618283205004 1103 10-11 -0.004407841214380426 1103 12-13 -0.039978683642388546 1103 14-15 0.10520882627125872 1103 16-17 -0.09599323703780271 1103 18-19 -0.06999065263332227 1103 20-21 -0.06660386357556547 1103 22-23 0.11910906848653013 1103 24-25 0.018954135549385853 1103 26-27 0.18522355881656694 1103 28-29 -0.008646918252210867 1103 30-31 0.07843946610916674 1103 32-33 0.06615896756552075 1103 34-35 -0.24792328474170944 1103 36-37 0.11597099397538102 1103 38-39 -0.22311661007667283 1103 40-41 -0.04141767426914811 1103 42-43 -0.07607246300775472 1103 44-45 0.08269519380762347 1103 46-47 0.13344813032283298 1103 48-49 0.01468705334835363 1103 50-51 -0.1871394322694826 1103 52-53 0.02723129004311886 1103 54-55 0.12166144212904584 1103 56-57 -0.28879923368413785 1103 58-59 0.06486597179312525 1103 60-61 0.01307089438793696 1103 62-63 0.1578737156048149 1103 64-65 0.20543056166430773 1103 66-67 -0.15652043657041048 1103 68-69 0.025799217410671815 1103 70-71 -0.29422314916841685 1103 72-73 -0.2798015819374555 1103 74-75 -0.5135005427820971 1103 76 -0.35443794271667883 1104 1 -0.12597897704550576 1104 2 -0.060071576919483505 1104 3 0.00893914762820458 1104 4 0.024390342052690528 1104 5 -0.20686949016340606 1104 6 -0.11773210762123654 1104 7 -0.2708583901928492 1104 8 -0.2928953452479348 1104 9 -0.08510501801087855 1104 10-11 -0.18258137069301483 1104 12-13 -0.17165201985575607 1104 14-15 -0.07543451971905313 1104 16-17 -0.0963456619485541 1104 18-19 -0.19914869815298175 1104 20-21 -0.09371784461283283 1104 22-23 -0.15921566264736242 1104 24-25 -0.23718203688871853 1104 26-27 -0.08780058797409396 1104 28-29 -0.1836400971249077 1104 30-31 -0.27950203135564067 1104 32-33 0.02750591283734849 1104 34-35 0.014417271407538124 1104 36-37 -0.0329654785923168 1104 38-39 -0.23146680675250764 1104 40-41 -0.18716811195815808 1104 42-43 -0.053400741127852314 1104 44-45 -0.11855079878009178 1104 46-47 -0.5290282799452442 1104 48-49 -0.11050621192528354 1104 50-51 -0.18545120326150766 1104 52-53 -0.26253019787211684 1104 54-55 -0.44255378717451777 1104 56-57 -0.8050960436702752 1104 58-59 -0.20794785690563344 1104 60-61 -0.15341861244456112 1104 62-63 -0.36938237225130166 1104 64-65 -0.5057316447430082 1104 66-67 -0.6619091271413353 1104 68-69 -0.42643299649988364 1104 70-71 -0.7504457225523851 1104 72-73 -0.38824045326825285 1104 74-75 -0.4071449574156887 1104 76 -0.08069473542261818 1105 1 -0.10267263693094719 1105 2 -0.15114124199665468 1105 3 -0.022132210403455588 1105 4 -0.0420129761381034 1105 5 0.07464626842128297 1105 6 0.039035477943507146 1105 7 0.0788947206110251 1105 8 -0.06558798936068655 1105 9 -0.08156674313149637 1105 10-11 0.060131871032666595 1105 12-13 0.06834685150791842 1105 14-15 -0.07365127432530016 1105 16-17 -0.005222386645947097 1105 18-19 -0.09970172995231508 1105 20-21 0.027816536471220843 1105 22-23 -0.15286426172752243 1105 24-25 -0.1619471394535381 1105 26-27 -0.06999634994469517 1105 28-29 -0.008994912169626446 1105 30-31 -0.10654415770648029 1105 32-33 0.005192772688928926 1105 34-35 -0.1823717582473776 1105 36-37 -0.09925593315059444 1105 38-39 -0.11905180717575092 1105 40-41 -0.07960342698876843 1105 42-43 -0.19574435445705518 1105 44-45 -0.2589588008116408 1105 46-47 -0.13348075024799755 1105 48-49 -0.1154129583489123 1105 50-51 -0.3270329870712132 1105 52-53 0.08230205855405615 1105 54-55 -0.24046863156378606 1105 56-57 -0.019381757955983403 1105 58-59 -0.030551620907772303 1105 60-61 0.026280041656619346 1105 62-63 -0.1248435612696639 1105 64-65 -0.07272185382288399 1105 66-67 -0.14967753096741632 1105 68-69 0.08840010640354024 1105 70-71 0.043942133320754806 1105 72-73 0.08872690092603364 1105 74-75 0.3498314258692119 1105 76 0.4011709004228692 1106 1 0.06801009399274704 1106 2 -0.0748982418269648 1106 3 0.03184708706812245 1106 4 0.023890072676877594 1106 5 0.13014714516770454 1106 6 0.03583959582347518 1106 7 -0.08479666464832292 1106 8 0.003335592289857914 1106 9 0.0566141494529262 1106 10-11 0.0010504750261048912 1106 12-13 0.11758889008476814 1106 14-15 -0.07959335249714172 1106 16-17 -0.004147665225048058 1106 18-19 0.172089660963465 1106 20-21 0.16155147623025812 1106 22-23 -0.21630959234577318 1106 24-25 -0.025675291498338026 1106 26-27 -0.1777088902467483 1106 28-29 0.09344866495577264 1106 30-31 -0.22222943402301354 1106 32-33 -0.02829611242901109 1106 34-35 -0.15414052344903695 1106 36-37 -0.025631859390770728 1106 38-39 0.15373511516148142 1106 40-41 0.09171282393040059 1106 42-43 0.1650905343941318 1106 44-45 -0.044464202700879696 1106 46-47 -0.26941321249134376 1106 48-49 -0.10337325022062771 1106 50-51 0.2132662379980772 1106 52-53 0.13948572278845717 1106 54-55 0.14437213997307197 1106 56-57 0.041651105893777185 1106 58-59 -0.037149844789219344 1106 60-61 0.1478480036454215 1106 62-63 0.049824123554138566 1106 64-65 0.20537928311925668 1106 66-67 0.1879688291208268 1106 68-69 0.19967336846586647 1106 70-71 0.17896956699912892 1106 72-73 0.37485450995369973 1106 74-75 0.11596638825395189 1106 76 0.4907008512119404 1107 1 -0.008180382197728875 1107 2 -0.12010280252447814 1107 3 -0.16174781568171426 1107 4 -0.10535204670608778 1107 5 -0.10214661740774744 1107 6 -0.022611108401875413 1107 7 -0.07601061501720352 1107 8 0.027010977937074188 1107 9 -0.08842273854170912 1107 10-11 0.05334759105561204 1107 12-13 0.1295774883409635 1107 14-15 0.247418719937464 1107 16-17 0.13683154335979708 1107 18-19 -0.035975396045259345 1107 20-21 0.14726576194453855 1107 22-23 0.0020841031605982607 1107 24-25 0.1978491887162832 1107 26-27 0.09170760874721395 1107 28-29 0.13369011364792982 1107 30-31 0.17356198113459698 1107 32-33 0.04559724773196194 1107 34-35 0.1271807374496916 1107 36-37 0.2821984558339139 1107 38-39 0.16656207693210945 1107 40-41 0.217886532850585 1107 42-43 0.002649219840137107 1107 44-45 0.0657973667156142 1107 46-47 0.07257203231080922 1107 48-49 0.12347114951109006 1107 50-51 -0.015271656032773251 1107 52-53 0.07035426738939776 1107 54-55 0.2877155336685746 1107 56-57 -0.012238900813123621 1107 58-59 0.3725751719780561 1107 60-61 0.07128864750860231 1107 62-63 0.13912660511013542 1107 64-65 0.30744165736472695 1107 66-67 0.17698626708192222 1107 68-69 0.19987457570933032 1107 70-71 0.046021880882435084 1107 72-73 -0.10002811915428111 1107 74-75 0.3962313111245095 1107 76 -0.023786070317235897 1108 1 -0.1527324403091086 1108 2 -0.034469227060398566 1108 3 -0.028293416973077967 1108 4 -0.04336657455112203 1108 5 0.018232720768203592 1108 6 -0.13491942155764747 1108 7 0.12544216948643339 1108 8 0.1350012927070523 1108 9 0.09458775379081885 1108 10-11 0.003085282742461004 1108 12-13 -0.06503510245806154 1108 14-15 0.08848005972761541 1108 16-17 0.11156923504663752 1108 18-19 0.09277763058912569 1108 20-21 0.08649094354260711 1108 22-23 -0.013957059067003286 1108 24-25 -0.1332444351739568 1108 26-27 -0.2190672096547175 1108 28-29 -0.01643095172737219 1108 30-31 0.034235911723783374 1108 32-33 0.07398707824043527 1108 34-35 0.10938412728020097 1108 36-37 0.0741476083762862 1108 38-39 -0.1543620554326992 1108 40-41 0.009553199517256417 1108 42-43 0.006119760598814139 1108 44-45 0.03242367825718162 1108 46-47 0.2018294980089479 1108 48-49 0.13535573385330224 1108 50-51 0.072298965436147 1108 52-53 0.17118154745396197 1108 54-55 0.14564531575089745 1108 56-57 0.13921388369050192 1108 58-59 0.10958082169556604 1108 60-61 0.18375838116477183 1108 62-63 -0.0506231931142338 1108 64-65 0.09733269852695514 1108 66-67 0.1375391322958066 1108 68-69 -0.06299870919785633 1108 70-71 0.3016917759591067 1108 72-73 -0.2124574976853495 1108 74-75 -0.020132692103899785 1108 76 0.6549064236294981 1109 1 -0.03292887314340476 1109 2 -0.11480434511334892 1109 3 -0.040922865983525014 1109 4 -0.18972495079730578 1109 5 0.05972460995644013 1109 6 -0.2223763666178371 1109 7 0.03022680953008461 1109 8 -0.25488037015145437 1109 9 0.0260977175280388 1109 10-11 -0.25481806957483855 1109 12-13 -0.12654256531430264 1109 14-15 -0.07724593465677287 1109 16-17 -0.14499273564758397 1109 18-19 0.004249285376602074 1109 20-21 -0.06027950968523044 1109 22-23 -0.07194339516267689 1109 24-25 -0.17473632436219333 1109 26-27 -0.00869480573970094 1109 28-29 -0.06030720358882746 1109 30-31 0.0864560119863782 1109 32-33 -0.1210191171238435 1109 34-35 -0.10719216664151787 1109 36-37 0.02601133309749315 1109 38-39 -0.0023681712234946417 1109 40-41 -0.1923247347550472 1109 42-43 -0.07904092100493187 1109 44-45 -0.038595658099943364 1109 46-47 -0.15556344723312066 1109 48-49 -0.10806808590137962 1109 50-51 -0.06490277608642714 1109 52-53 -0.07999784528666254 1109 54-55 -0.42135555955745474 1109 56-57 -0.17196391758039908 1109 58-59 -0.025412755587339575 1109 60-61 -0.029382043302938143 1109 62-63 -0.26238244921581355 1109 64-65 -0.21832963706854258 1109 66-67 -0.26977764975241314 1109 68-69 -0.1935191197970454 1109 70-71 0.009955482492088663 1109 72-73 -0.31293891727634815 1109 74-75 -0.11290685118265742 1109 76 -0.171270979773972 1110 1 0.01987962462122539 1110 2 -0.020761968338781855 1110 3 0.0019469223567014637 1110 4 0.13965306751143913 1110 5 0.0641393241765229 1110 6 -0.0786572500088738 1110 7 -0.08446612132473774 1110 8 0.14995676723131623 1110 9 -0.1328880186871828 1110 10-11 -0.05891232484391651 1110 12-13 0.10217380610435356 1110 14-15 -0.017375574868765398 1110 16-17 0.12134114672381457 1110 18-19 0.21297618936218043 1110 20-21 0.08159920892715888 1110 22-23 -0.09495817437093734 1110 24-25 0.1824241404153426 1110 26-27 0.06635813721636197 1110 28-29 0.1596021491065045 1110 30-31 -0.06426333992461508 1110 32-33 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52-53 -0.11297906594393936 2115 54-55 0.0067631527161893246 2115 56-57 0.015142051567828219 2115 58-59 -0.1917105423076606 2115 60-61 0.03795531417527087 2115 62-63 -0.0501591091755742 2115 64-65 0.12410832403139693 2115 66-67 0.2865100766057296 2115 68-69 0.22130314713790256 2115 70-71 -0.10159716673661023 2115 72-73 0.19878140465523586 2115 74-75 0.0021836920768976142 2115 76 0.3214520249208661 2116 1 0.04303056901321867 2116 2 0.06689848447680902 2116 3 0.051139747205851904 2116 4 0.028659887305842346 2116 5 0.08645172119059197 2116 6 -0.013479249270012872 2116 7 0.0049300659234816635 2116 8 0.055722806648901724 2116 9 0.16660826648929117 2116 10-11 0.0963948591028867 2116 12-13 0.04538127914475609 2116 14-15 0.20237074988949644 2116 16-17 -0.18227457574632666 2116 18-19 0.043973708903848774 2116 20-21 0.03992985460863707 2116 22-23 0.11857234464883959 2116 24-25 0.07740634327343798 2116 26-27 0.11362758066719181 2116 28-29 0.046817526775342344 2116 30-31 0.032445215017830265 2116 32-33 -0.038669161534450325 2116 34-35 0.044853605122561646 2116 36-37 -0.02686612823067236 2116 38-39 0.2314825168525516 2116 40-41 -0.03958684962279335 2116 42-43 -0.14542612823520784 2116 44-45 0.03308998400822816 2116 46-47 0.20787681761559185 2116 48-49 -0.10983585879591828 2116 50-51 -0.10419174495286398 2116 52-53 0.01062863266376013 2116 54-55 0.020858741811778714 2116 56-57 -0.06606480463902642 2116 58-59 0.08738153678442018 2116 60-61 8.604270803758141E-4 2116 62-63 0.19238505836859332 2116 64-65 0.1175241424472091 2116 66-67 0.14511836021402047 2116 68-69 -0.14774447190971784 2116 70-71 0.026257636118188543 2116 72-73 -0.1792254983516557 2116 74-75 0.09754463743784214 2116 76 -0.07752422405538795 2117 1 -0.13361592226741692 2117 2 -0.06098549706571532 2117 3 -0.1400869562159741 2117 4 0.03262704737056765 2117 5 0.06306824902662811 2117 6 0.01681978590706734 2117 7 0.059529454669210224 2117 8 -0.4970308339096121 2117 9 -0.21600694295518252 2117 10-11 0.053666987106716135 2117 12-13 -0.06388941061373288 2117 14-15 -0.04279614649691155 2117 16-17 -0.1324483637249898 2117 18-19 -0.10644577932051646 2117 20-21 -0.1543021823063242 2117 22-23 0.040847169362685065 2117 24-25 -0.2573482561153142 2117 26-27 0.053380082614815194 2117 28-29 -0.6917454264217611 2117 30-31 -0.0520187389583171 2117 32-33 -0.1459532749161241 2117 34-35 -0.01898650993707207 2117 36-37 -0.10342872186643604 2117 38-39 -0.06650993235940916 2117 40-41 -0.12581265414127785 2117 42-43 0.010779301140949826 2117 44-45 0.10348610074581188 2117 46-47 0.14324566761858648 2117 48-49 0.07280332140423695 2117 50-51 0.20726028357233872 2117 52-53 0.1067363812105313 2117 54-55 0.19651344307624186 2117 56-57 -0.24011346527305477 2117 58-59 0.16502581077015321 2117 60-61 -0.04299706677711157 2117 62-63 0.12547968292663114 2117 64-65 -0.0780113043077506 2117 66-67 -0.0038209338472299237 2117 68-69 0.08762138174881784 2117 70-71 0.16864092850148182 2117 72-73 -0.00937771265022036 2117 74-75 0.38888520194913667 2117 76 -0.5952146417458053 2118 1 -0.02734247010982216 2118 2 -0.22227771094939186 2118 3 -0.20176613070002958 2118 4 -0.20720644231048624 2118 5 -0.01874460275573142 2118 6 0.09293376339299897 2118 7 0.12550037399378766 2118 8 -0.07381319788709817 2118 9 -0.034393434512409726 2118 10-11 -0.010709185501163176 2118 12-13 0.1525857300992115 2118 14-15 0.006324397593147069 2118 16-17 0.02514427229752414 2118 18-19 -0.12313198945184922 2118 20-21 -0.21344852376974188 2118 22-23 -0.01989391881742364 2118 24-25 -0.07703634241925528 2118 26-27 0.010011180175787615 2118 28-29 0.02534533342814882 2118 30-31 -0.020176571978957725 2118 32-33 -0.2633313236966117 2118 34-35 -0.11145250430855214 2118 36-37 -0.05372187383641602 2118 38-39 -0.006572080577051054 2118 40-41 0.0011397379787894124 2118 42-43 0.06402742496833724 2118 44-45 -0.020786882368632575 2118 46-47 -0.14067201530824036 2118 48-49 -0.013330590415648658 2118 50-51 0.02822651246539465 2118 52-53 -0.121072014661884 2118 54-55 -0.029489136661098314 2118 56-57 -0.1600067579559834 2118 58-59 -0.03400678772891297 2118 60-61 -0.12160283600788091 2118 62-63 -0.028272662289133166 2118 64-65 -0.06398691406384671 2118 66-67 -0.2507838794382238 2118 68-69 -0.38211947190971784 2118 70-71 -0.6068398407292932 2118 72-73 -0.5760697858209483 2118 74-75 -0.2585878280696221 2118 76 -0.5745415648227237 2119 1 0.06352761640513194 2119 2 0.02381383503518464 2119 3 0.01788310759224032 2119 4 0.10547575629774997 2119 5 -0.1818241118774182 2119 6 0.10619899754759388 2119 7 0.0047438383533915385 2119 8 0.04817758638974823 2119 9 -0.18363172678248674 2119 10-11 -0.15424642454573245 2119 12-13 -0.11199603593426133 2119 14-15 -0.635067599773798 2119 16-17 -0.11230106812000429 2119 18-19 0.0347284111499917 2119 20-21 0.07475441021558993 2119 22-23 0.1187886089237864 2119 24-25 0.0032427153200771386 2119 26-27 0.21435867406331965 2119 28-29 0.07850324672511988 2119 30-31 0.02530549027419937 2119 32-33 -0.19838633592154764 2119 34-35 -0.07727263314059485 2119 36-37 -6.080408478936761E-4 2119 38-39 0.18229440898473115 2119 40-41 0.3322915858253239 2119 42-43 -0.021882157502979283 2119 44-45 0.15796758443595138 2119 46-47 0.08277694557195048 2119 48-49 -0.07457577096859325 2119 50-51 0.15529534827506097 2119 52-53 0.011694935686058727 2119 54-55 0.022111272404167437 2119 56-57 0.08330772859657998 2119 58-59 0.10300362697863363 2119 60-61 0.13047481586347232 2119 62-63 0.32982400876597495 2119 64-65 -0.05267884560419134 2119 66-67 0.17883456141188248 2119 68-69 0.11753670168441488 2119 70-71 0.37120816566529413 2119 72-73 0.17755949290766182 2119 74-75 0.457690735970246 2119 76 -1.0942366466357782 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 4.0 22 5.0 23 8.0 24 26.0 25 76.0 26 125.0 27 219.0 28 345.0 29 682.0 30 1057.0 31 1659.0 32 2649.0 33 4358.0 34 7171.0 35 12180.0 36 20362.0 37 107153.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.21304900652197 16.797085038682702 11.38720023279205 30.602665722003273 2 21.977340730385055 20.05301079826165 32.53585187340667 25.433796597946625 3 19.750634168559156 19.527963512376566 31.302307045122436 29.419095273941842 4 15.416147418095786 20.92092028770061 33.36264320190282 30.30028909230078 5 15.601495435884136 30.562812735243323 36.595163239098945 17.240528589773596 6 28.320291496131727 36.80328439217869 18.204591317109582 16.671832794579995 7 28.070419595017743 26.610408588002354 21.850190725008066 23.468981091971838 8 25.00300478868428 34.92829625318666 17.72002960507588 22.348669353053182 9 23.493651988537522 17.235467893042173 18.907395575685882 40.363484542734426 10-11 26.32606069040555 25.493576078086548 24.13383012506247 24.04653310644543 12-13 25.474914758889426 20.31205521220134 25.663742005680636 28.5492880232286 14-15 22.95500408018674 20.119116149315857 21.910286498693708 35.015593271803695 16-17 26.313092655031284 25.331633782681028 21.639855517108316 26.71541804517937 18-19 27.287909362921543 24.349858616785067 22.401174081641688 25.961057938651706 20-21 24.242002517696623 23.28679600964063 22.251567234519012 30.21963423814374 22-23 24.340053516867936 25.336061892321027 21.92736635016226 28.39651824064878 24-25 26.784686331690715 26.092952347214403 21.854935128193777 25.26742619290111 26-27 26.612306349276636 26.850791682744923 22.334436143496056 24.202465824482385 28-29 27.077890448567505 23.721383341451535 23.04894326326377 26.15178294671719 30-31 27.024120545796144 25.40185094982952 23.04989214390091 24.524136360473427 32-33 24.38813013581645 25.72035855036342 23.09828505639514 26.79322625742499 34-35 25.28798527337251 23.87573459175992 23.45538046950614 27.38089966536143 36-37 24.973905782478603 24.245481746699475 26.092319760122972 24.688292710698946 38-39 22.415407291198814 24.471947925430634 27.508998551375562 25.603646231994993 40-41 22.69121526306134 21.54053934375415 27.98660180540356 27.781643587780948 42-43 25.232317609326866 21.028143799697624 27.551381886501225 26.188156704474284 44-45 21.947609137087948 23.886804865859908 29.632593417298725 24.53299257975342 46-47 23.753645283114352 22.129794219419157 28.034362130806358 26.08219836666013 48-49 23.340882205957705 21.4643125992371 26.61199005573092 28.582815139074274 50-51 21.617714968908345 22.28572693745611 28.397467121285924 27.699090972349616 52-53 19.175928795996988 22.36638179161316 32.43432164523251 26.023367767157346 54-55 19.083254787102817 22.868655942206843 30.03207216553539 28.01601710515495 56-57 21.5142869794599 23.11283455949798 26.668290306868 28.704588154174125 58-59 20.07546764000734 23.123904833597965 29.546561572864544 27.254065953530155 60-61 22.012133020413586 25.18424099037835 26.629069907199476 26.174556082008593 62-63 19.835400838810482 29.79168907079282 23.578734952334564 26.794175138062133 64-65 19.478938012790913 31.4901854112765 22.12504981623345 26.905826759699142 66-67 19.806618126150518 31.060975069742724 21.560149543588413 27.57225726051834 68-69 19.744940884736305 31.54300643341072 21.518398795554177 27.193653886298797 70-71 20.162764658624376 30.930978422454313 21.29509555228016 27.611161366641152 72-73 20.896881978226354 29.34444999715336 21.444386105857124 28.314281918763168 74-75 21.24259082369165 28.784294127694032 21.49973747635706 28.47337757225726 76 21.47063847015138 28.987670877588073 21.24986557524307 28.291825077017478 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.5 14 3.5 15 4.0 16 4.0 17 6.0 18 13.0 19 22.0 20 35.0 21 46.0 22 53.5 23 69.5 24 108.0 25 138.0 26 207.0 27 345.0 28 463.5 29 513.0 30 665.0 31 966.0 32 1376.0 33 1637.0 34 1886.5 35 2477.5 36 3019.5 37 3220.0 38 3742.0 39 4839.5 40 6026.5 41 8323.0 42 10008.0 43 10850.0 44 11652.0 45 10778.0 46 9944.0 47 9619.0 48 8808.5 49 7827.0 50 7331.0 51 6836.5 52 5743.0 53 4721.5 54 4299.0 55 4009.0 56 3339.5 57 2783.5 58 2607.0 59 2396.5 60 2226.0 61 2390.0 62 2514.0 63 2590.0 64 2821.5 65 2956.0 66 2988.5 67 3042.0 68 3055.5 69 2935.0 70 2683.0 71 2565.0 72 2424.0 73 2133.5 74 1745.5 75 1507.0 76 1287.5 77 878.0 78 541.0 79 394.0 80 268.5 81 96.5 82 31.0 83 12.0 84 8.5 85 3.0 86 0.5 87 0.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 158081.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.59106407474649 #Duplication Level Percentage of deduplicated Percentage of total 1 62.93091218612569 28.06156337573775 2 19.263725351113635 17.179800228996527 3 6.745637679103419 9.023854859217744 4 3.23166406582494 5.7641335770902264 5 1.8768619662363455 4.184563609794979 6 1.2682650021279613 3.3931971584187854 7 0.9277911760533408 2.895983704556525 8 0.7235068804085686 2.580955333025474 9 0.5362462760675273 2.1520612850374174 >10 2.4712725209249538 16.498503931528774 >50 0.011349127535820683 0.3283127004510346 >100 0.007093204709887927 0.7116604778562889 >500 0.0 0.0 >1k 0.004255922825932757 3.12561281874482 >5k 0.0014186409419775854 4.099796939543652 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 6481 4.099796939543652 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 1961 1.2405032862899399 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 1682 1.0640114877815803 No Hit CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG 1298 0.8210980446733005 TruSeq Adapter, Index 2 (95% over 24bp) TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 450 0.28466419114251557 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 247 0.15624901158266966 TruSeq Adapter, Index 2 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT 194 0.12272189573699559 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00822363218856156 0.0 2 0.0 0.0 0.0 0.012019154737128434 0.0 3 0.0 0.0 0.0 0.012019154737128434 0.0 4 0.0 0.0 0.0 0.049974380222797175 0.0 5 0.0 0.0 0.0 0.05693283822850311 0.0 6 0.0 0.0 0.0 0.05756542531993092 0.0 7 0.0 0.0 0.0 0.05946318659421436 0.0 8 0.0 0.0 0.0 0.06009577368564217 0.0 9 0.0 0.0 0.0 0.06515647041706467 0.0 10 0.0 0.0 0.0 0.06705423169134811 0.0 11 0.0 0.0 0.0 0.06958458005705935 0.0 12 0.0 0.0 0.0 0.06958458005705935 0.0 13 0.0 0.0 0.0 0.06958458005705935 0.0 14 0.0 0.0 0.0 0.0721149284227706 0.0 15 0.0 0.0 0.0 0.0721149284227706 0.0 16 0.0 0.0 0.0 0.07274751551419842 0.0 17 0.0 0.0 0.0 0.07401268969705405 0.0 18 0.0 0.0 0.0 0.07464527678848186 0.0 19 0.0 0.0 0.0 0.07654303806276529 0.0 20 0.0 0.0 0.0 0.0822363218856156 0.0 21 0.0 0.0 0.0 0.08286890897704341 0.0 22 0.0 0.0 0.0 0.08413408315989904 0.0 23 0.0 0.0 0.0 0.08856219279989372 0.0 24 0.0 0.0 0.0 0.0923577153484606 0.0 25 0.0 0.0 0.0 0.10058134753702216 0.0 26 0.0 0.0 0.0 0.13031294083412934 0.0 27 0.0 0.0 0.0 0.24734155274827463 0.0 28 0.0 0.0 0.0 0.33969926809673523 0.0 29 0.0 0.0 0.0 0.4586256412851639 0.0 30 0.0 0.0 0.0 0.552881117907908 0.0 31 0.0 0.0 0.0 0.6838266458334651 0.0 32 0.0 0.0 0.0 0.8438711799647016 0.0 33 0.0 0.0 0.0 1.010874172101644 0.0 34 0.0 0.0 0.0 1.2253211960956725 0.0 35 0.0 0.0 0.0 1.5511035481809958 0.0 36 0.0 0.0 0.0 2.067294614786091 0.0 37 0.0 0.0 0.0 2.824501363225182 0.0 38 0.0 0.0 0.0 3.734161600698376 0.0 39 0.0 0.0 0.0 4.621681289971597 0.0 40 0.0 0.0 0.0 5.656593771547498 0.0 41 0.0 0.0 0.0 6.749704265534757 0.0 42 0.0 0.0 0.0 7.9977985969218315 0.0 43 0.0 0.0 0.0 9.229445663931783 0.0 44 0.0 0.0 0.0 10.419341982907497 0.0 45 0.0 0.0 0.0 11.559263921660415 0.0 46 0.0 0.0 0.0 12.670086854207653 0.0 47 0.0 0.0 0.0 13.690449832680715 0.0 48 0.0 0.0 0.0 14.643758579462428 0.0 49 0.0 0.0 0.0 15.535706378375643 0.0 50 0.0 0.0 0.0 16.37008875196893 0.0 51 0.0 0.0 0.0 17.286707447447828 0.0 52 0.0 0.0 0.0 18.183715943092466 0.0 53 0.0 0.0 0.0 19.149676431702733 0.0 54 0.0 0.0 0.0 20.048582688621657 0.0 55 0.0 0.0 0.0 20.939265313352017 0.0 56 0.0 0.0 0.0 21.805277041516693 0.0 57 0.0 0.0 0.0 22.523895977378686 0.0 58 0.0 0.0 0.0 23.238719390692115 0.0 59 0.0 0.0 0.0 23.989600268216925 0.0 60 0.0 0.0 0.0 24.680385372056097 0.0 61 0.0 0.0 0.0 25.347132166421012 0.0 62 0.0 0.0 0.0 26.031591399345906 0.0 63 0.0 0.0 0.0 26.790063321967853 0.0 64 0.0 0.0 0.0 27.50804967073842 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGGG 15 0.0022213461 70.0 17 AAATGGA 15 0.0022213461 70.0 14 ACGTAGC 25 2.3847105E-4 56.000004 33 TAGCTCG 25 2.3847105E-4 56.000004 36 CTTTATA 100 0.0 52.500004 2 TAAACCG 20 0.006941004 52.5 12 ATAGACC 20 0.006941004 52.5 14 AGTCTTG 20 0.006941004 52.5 5 CAGTCTT 20 0.006941004 52.5 4 CGACTAA 20 0.006941004 52.5 37 GCGCTTT 20 0.006941004 52.5 29 GATAGAC 20 0.006941004 52.5 13 CGAAGAT 20 0.006941004 52.5 17 CTTTTAG 30 5.863941E-4 46.666664 1 TCTTTAT 115 0.0 45.652172 1 TTTATAC 160 0.0 37.1875 3 CGTCTTC 1585 0.0 35.77287 48 CCGTCTT 1585 0.0 35.77287 47 TCGTATG 1565 0.0 35.55911 40 CGTATGC 1575 0.0 35.555557 41 >>END_MODULE