FastQCFastQC Report
Wed 25 May 2016
SRR1780327_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780327_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747148
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAA454.0960484E-529.303261
CGAAACT407.0332247E-427.49757225
TCTAAAG500.002558833722.03052721
GTCAGTA703.2154192E-521.99805829
ACAGTCG500.002595940821.9759754
TACCACG654.886056E-420.33450720
CACGCTA654.928213E-420.3086223
CATCGTT654.950524E-420.29502132
CTATAAC654.966192E-420.2855154
CACAGTC654.966192E-420.2855153
TTGTACA550.004508718419.9794962
TTCCCTA708.124169E-418.85547826
CGTTAAT600.007384683518.34400624
CGTCAAA600.007396113618.33908712
CTAAAGA600.007410421518.33294322
ACAAGTG600.00741328618.33171527
CGTTGGA600.00745348218.3145391
TAACCGG1002.4814044E-517.59962522
GAATAGA750.001299146717.5819551
TTGTAAG902.2154355E-417.109626
Produced by FastQC (version 0.11.5)