FastQCFastQC Report
Wed 25 May 2016
SRR1780327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747148
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT209842.808546633331013No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC63650.8519061819077346No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG55500.742824714782078No Hit
CACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTTCTG37000.49521647652138534RNA PCR Primer, Index 31 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC12520.16757054827156065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT5650.032.7068241
ATGCCGT30850.032.66012244
GTCTTCT5850.031.96469743
TCTTCTG6000.031.16557744
CGTCTTC6100.030.65466542
CTTTATA2600.030.4604742
GCCGTCT6100.030.29402240
TCTTTAT2700.029.3932951
GATCATA300.00568658729.3932931
CACAAGT300.005744607729.332312
CCGAATT300.00574648729.33034514
GAACCGA300.00574648729.33034511
TATGCCG35250.028.45857843
TTAATAC801.308872E-724.7491343
GAAAACT450.001397999424.44358842
GTCATTC651.8136181E-523.68989432
CGTATGC45850.021.87927841
AATTCGT45650.021.87728929
TCGTTAT45250.021.87622332
GTTATCT45250.021.8276134
Produced by FastQC (version 0.11.5)