FastQCFastQC Report
Wed 25 May 2016
SRR1780326_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780326_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131361
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCA207.902541E-570.0000135
CCCATTG150.00222050870.024
GAGTTAG200.00693840452.5000041
GAACCGT200.00693840452.50000432
TAGGATC350.00125171340.024
TCCCGAA400.002411666335.00000432
CCGAATC400.002411666335.00000434
GGACAGC400.002411666335.0000046
CCCGAAT400.002411666335.00000433
TCATAAA450.004294716331.11110959
GGATCCT604.9605814E-429.16666626
CGTATCA500.00718750527.99999857
CTCCCGA500.00718750527.99999831
GAATCAA500.00718750527.99999836
TTCATAA657.921658E-426.92307958
ATATCTC750.001824441523.33333440
GGATTAG750.001824441523.3333341
ACGACGG901.9162487E-423.3333325
TATATCT1154.0839066E-521.30434839
ATGATAT900.00525251219.44444318