##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780326_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 131361 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.994823425522036 34.0 31.0 34.0 12.0 34.0 2 30.17002763377258 34.0 31.0 34.0 12.0 34.0 3 29.985741582357015 34.0 31.0 34.0 11.0 34.0 4 29.85140186204429 34.0 31.0 34.0 11.0 34.0 5 29.67736999566081 34.0 31.0 34.0 11.0 34.0 6 32.76575239226254 37.0 33.0 38.0 11.0 38.0 7 32.92503102138382 37.0 34.0 38.0 12.0 38.0 8 32.86691636025913 37.0 34.0 38.0 11.0 38.0 9 33.31403536818386 38.0 34.0 38.0 12.0 38.0 10-11 33.02694483141876 38.0 34.0 38.0 11.0 38.0 12-13 33.138633232085624 38.0 34.0 38.0 11.0 38.0 14-15 33.0789503733985 38.0 34.0 38.0 11.0 38.0 16-17 33.11902314994557 38.0 34.0 38.0 11.0 38.0 18-19 33.13635706183723 38.0 34.0 38.0 11.0 38.0 20-21 33.15982673700718 38.0 34.0 38.0 11.0 38.0 22-23 33.1615319615411 38.0 34.0 38.0 11.0 38.0 24-25 33.167983648114735 38.0 34.0 38.0 11.0 38.0 26-27 33.14617732812631 38.0 34.0 38.0 11.0 38.0 28-29 33.24119411393031 38.0 34.0 38.0 11.0 38.0 30-31 33.26273018628056 38.0 34.0 38.0 11.0 38.0 32-33 33.30222059819886 38.0 34.0 38.0 11.0 38.0 34-35 33.27296914609359 38.0 34.0 38.0 11.0 38.0 36-37 33.19564406482898 38.0 34.0 38.0 11.0 38.0 38-39 33.20215284597407 38.0 34.0 38.0 11.0 38.0 40-41 33.27481520390374 38.0 34.0 38.0 11.0 38.0 42-43 33.31737730376596 38.0 34.0 38.0 11.0 38.0 44-45 33.396875785050355 38.0 34.0 38.0 11.0 38.0 46-47 33.297759609016374 38.0 34.0 38.0 11.0 38.0 48-49 33.25574561704006 38.0 34.0 38.0 11.0 38.0 50-51 33.204878160184535 38.0 34.0 38.0 11.0 38.0 52-53 33.247314651989555 38.0 34.0 38.0 11.0 38.0 54-55 33.211870342034544 38.0 34.0 38.0 11.0 38.0 56-57 33.161330227388646 38.0 34.0 38.0 11.0 38.0 58-59 33.20775192028076 38.0 34.0 38.0 11.0 38.0 60-61 33.24406787402654 38.0 34.0 38.0 11.0 38.0 62-63 33.119350492155206 38.0 34.0 38.0 11.0 38.0 64-65 33.05132421342712 38.0 33.5 38.0 11.0 38.0 66-67 32.928258767823024 38.0 32.5 38.0 11.0 38.0 68-69 32.86008784951393 38.0 32.5 38.0 11.0 38.0 70-71 32.80745046094351 38.0 32.5 38.0 11.0 38.0 72-73 32.76969953030199 38.0 33.0 38.0 11.0 38.0 74-75 32.79268961107178 38.0 32.5 38.0 11.0 38.0 76 31.18960726547453 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.008574173395484053 1101 2 -0.02466855889148789 1101 3 0.38149302563222065 1101 4 0.1877780332908614 1101 5 -0.32408232677046556 1101 6 0.08081366435864368 1101 7 0.06824155439134927 1101 8 -0.2824734698670639 1101 9 0.1566725732724521 1101 10-11 -0.03032240263861752 1101 12-13 -0.2103885632137903 1101 14-15 -0.3348386777994463 1101 16-17 0.03895455371804246 1101 18-19 -0.5122742245376273 1101 20-21 -0.40034333816282697 1101 22-23 -0.022244141569601084 1101 24-25 -0.40856029789991055 1101 26-27 -0.2842752408914393 1101 28-29 -0.6080329226921748 1101 30-31 -0.9554420761126892 1101 32-33 -0.27245147830247163 1101 34-35 -0.5057054327143717 1101 36-37 -0.3284770297102213 1101 38-39 -0.467854852014149 1101 40-41 -0.17944418048002575 1101 42-43 -0.7942267750158081 1101 44-45 -0.5364840074032955 1101 46-47 -0.1574602892654724 1101 48-49 -0.41328189659837733 1101 50-51 -0.6191145609895656 1101 52-53 -0.23578224954452764 1101 54-55 -0.7108906692733328 1101 56-57 -9.211585732273875E-4 1101 58-59 -0.3366964395398071 1101 60-61 -0.658464956295127 1101 62-63 -0.029395831825510754 1101 64-65 -0.711508741510599 1101 66-67 -0.4436088646295602 1101 68-69 -0.5972052606358886 1101 70-71 -0.38829088878832607 1101 72-73 -0.40288153674847393 1101 74-75 -1.10375483536113 1101 76 -2.1508534682543505 1102 1 0.6387285018256748 1102 2 0.42213033187292126 1102 3 0.30943038999199857 1102 4 0.7252825546315904 1102 5 0.32989193172965514 1102 6 0.17169261299621752 1102 7 -0.4797298707352482 1102 8 -0.234328726679216 1102 9 0.3707505211866078 1102 10-11 0.16813808084341986 1102 12-13 0.5384471915486841 1102 14-15 0.35982764725481076 1102 16-17 0.49760312497437553 1102 18-19 0.21731568985831728 1102 20-21 0.15375450365053211 1102 22-23 0.3286005955897906 1102 24-25 0.4960243415985204 1102 26-27 -0.12167652495219983 1102 28-29 0.3258801469967594 1102 30-31 0.437793998157872 1102 32-33 0.31024761140093204 1102 34-35 0.17646692079419068 1102 36-37 0.23626103395025666 1102 38-39 0.0792123049668021 1102 40-41 0.13345897240157711 1102 42-43 0.15794874343125542 1102 44-45 0.05024284333217821 1102 46-47 0.19879875341597852 1102 48-49 -0.05061385343464053 1102 50-51 0.34312847614571496 1102 52-53 0.25057083702407823 1102 54-55 0.13460381469096916 1102 56-57 0.01667816021143409 1102 58-59 0.2744682578317921 1102 60-61 0.35984270587029243 1102 62-63 -0.07227698084890477 1102 64-65 0.2555721302038947 1102 66-67 0.41277828270585815 1102 68-69 0.38230929808126035 1102 70-71 0.03294358794183694 1102 72-73 0.2769585585011072 1102 74-75 -0.03614195255428143 1102 76 0.4961880677958064 1103 1 -0.30668738069288537 1103 2 0.3954050825508695 1103 3 0.051995184088287516 1103 4 0.7943518017012252 1103 5 0.2400841282828594 1103 6 -0.16772620359463275 1103 7 0.4218157037057182 1103 8 -0.5291881727619341 1103 9 0.15748199429835097 1103 10-11 -0.21306903798650723 1103 12-13 -0.2818001299989632 1103 14-15 -0.2282649093890825 1103 16-17 -0.34300488000466345 1103 18-19 -0.122125354552864 1103 20-21 0.20556384822620544 1103 22-23 0.046818517140408744 1103 24-25 -0.13396024711271082 1103 26-27 -0.09737040341047987 1103 28-29 -0.06775232340319803 1103 30-31 -0.23337503700653883 1103 32-33 0.04051671621251529 1103 34-35 0.022179915177964915 1103 36-37 0.06659978593223315 1103 38-39 -0.6641156442944975 1103 40-41 -0.09566910429922615 1103 42-43 -0.3493864372809199 1103 44-45 0.07878706928537582 1103 46-47 0.025484733588768904 1103 48-49 -0.17162210736526617 1103 50-51 -0.7745789464644233 1103 52-53 -0.09448273202293933 1103 54-55 -0.18312435388481418 1103 56-57 0.24162740668832328 1103 58-59 -0.0927909037348229 1103 60-61 0.09325704725997497 1103 62-63 -0.011294955746279811 1103 64-65 -0.04044316253649782 1103 66-67 -0.05403928616333076 1103 68-69 -0.06685197216458505 1103 70-71 -0.43529285679239393 1103 72-73 -0.17687055401611929 1103 74-75 -0.3400184844215701 1103 76 -0.10128833366826484 1104 1 -0.5371196065088206 1104 2 -0.1900991809010577 1104 3 -0.6241588882433788 1104 4 0.25018832037220307 1104 5 -1.0700452454307552 1104 6 -0.46978442176575896 1104 7 -0.3614833259914292 1104 8 -0.5996086373311798 1104 9 -0.663453024813677 1104 10-11 -0.07016989067689394 1104 12-13 -0.8668927498166639 1104 14-15 -0.6487441801917981 1104 16-17 -0.8214670969996618 1104 18-19 -0.6592688417625041 1104 20-21 -0.9211268309857985 1104 22-23 -0.6541370841533336 1104 24-25 -0.5376870921582935 1104 26-27 -0.408602394001484 1104 28-29 -0.673044884414665 1104 30-31 -0.5817578655863684 1104 32-33 -0.3087702582067777 1104 34-35 -0.46117792075264674 1104 36-37 -0.8261743981312719 1104 38-39 -0.5412400194782663 1104 40-41 -0.7598120034465339 1104 42-43 -0.9169989042024156 1104 44-45 -0.12706415094396561 1104 46-47 -0.41981076773437564 1104 48-49 -0.46398464779454685 1104 50-51 -0.6561211399369355 1104 52-53 -0.8123671777741919 1104 54-55 -0.4971496405652047 1104 56-57 -0.4534181681426048 1104 58-59 -0.4292801715972203 1104 60-61 -0.6171222529458404 1104 62-63 -0.5252540854140264 1104 64-65 -0.31424498552974 1104 66-67 -0.47034331439032684 1104 68-69 -0.42428360991818437 1104 70-71 0.08708291503942434 1104 72-73 -0.15352447933219793 1104 74-75 -0.2407315100022842 1104 76 -0.10925657830219748 1105 1 -0.7337116087075302 1105 2 -0.4507010772869293 1105 3 0.10700780132649967 1105 4 0.34389451798110926 1105 5 -0.10862570441110364 1105 6 0.522808679787083 1105 7 -0.29208659656996616 1105 8 0.048666750878247456 1105 9 -0.3681390217904479 1105 10-11 0.052957771821390054 1105 12-13 -0.7763265824399923 1105 14-15 -0.38041932104654563 1105 16-17 -0.3626928768539983 1105 18-19 -0.2910504825485489 1105 20-21 -0.3655922700403522 1105 22-23 -0.41129662792136656 1105 24-25 -0.3881027854384911 1105 26-27 -0.48998895439725487 1105 28-29 -0.11846195188751807 1105 30-31 -0.20413659308397314 1105 32-33 -0.5695267512671052 1105 34-35 -0.09274999715776033 1105 36-37 -0.37588238219063896 1105 38-39 -0.31515784275708825 1105 40-41 -0.11036855147456492 1105 42-43 -0.15526329887055113 1105 44-45 -0.5117762575813174 1105 46-47 -0.3733767976917761 1105 48-49 -0.3460754819294891 1105 50-51 -0.26112663670757996 1105 52-53 -0.3416821427322816 1105 54-55 -0.13989390331084195 1105 56-57 -0.045392882997632 1105 58-59 0.10253925948701692 1105 60-61 -0.11272483642805042 1105 62-63 -0.14084773664392713 1105 64-65 -0.21456104984195434 1105 66-67 -0.026314181505895817 1105 68-69 -0.27568185095157816 1105 70-71 -0.24082693792195897 1105 72-73 -0.32922188210807235 1105 74-75 -0.6575207088479544 1105 76 -1.2091329009626364 1106 1 0.27539593431173515 1106 2 0.563347427746546 1106 3 0.9314289001251801 1106 4 0.8826375536917226 1106 5 0.6186893864244745 1106 6 0.8601920943276227 1106 7 0.0755936183378978 1106 8 0.4680111996628824 1106 9 0.9123171091827373 1106 10-11 -0.02278765556154383 1106 12-13 0.5260966133550511 1106 14-15 0.5929604751473434 1106 16-17 0.9324353757175601 1106 18-19 0.3538786924272941 1106 20-21 0.8144547410545471 1106 22-23 1.0532189042428541 1106 24-25 0.7639671418519356 1106 26-27 0.3637763875981861 1106 28-29 0.4108617325213828 1106 30-31 0.4625369265119801 1106 32-33 0.8317636553171894 1106 34-35 0.6443407973023341 1106 36-37 0.6269855687149786 1106 38-39 0.838978242712642 1106 40-41 0.6577180421398694 1106 42-43 0.6947637702001259 1106 44-45 0.5716718623175367 1106 46-47 0.715608489069055 1106 48-49 0.8856734541532347 1106 50-51 0.7793844547584641 1106 52-53 0.49495772898088575 1106 54-55 0.08362809116569991 1106 56-57 0.4055264374659018 1106 58-59 0.6133154907870875 1106 60-61 0.47577731881820995 1106 62-63 0.6989170708230219 1106 64-65 0.5406797278284614 1106 66-67 0.49296653503638765 1106 68-69 0.5025881955798184 1106 70-71 0.5493843378878793 1106 72-73 0.6953013253369491 1106 74-75 0.5964248651876645 1106 76 0.8432783022061585 1107 1 0.4488546376121185 1107 2 0.9049736074528063 1107 3 0.4297884801776739 1107 4 -0.32122825297606283 1107 5 0.5772111935390107 1107 6 -1.1290509391229797 1107 7 0.26057714433331824 1107 8 0.22471947017162108 1107 9 0.20496802781790535 1107 10-11 0.2045361563555801 1107 12-13 0.9232248313703479 1107 14-15 0.26918695082724753 1107 16-17 0.6667047954975374 1107 18-19 0.5932927483830639 1107 20-21 0.5216223291485846 1107 22-23 1.0572710905387197 1107 24-25 0.8340654274385741 1107 26-27 0.5405857358977286 1107 28-29 0.7927165937488354 1107 30-31 0.5817919664405053 1107 32-33 0.4607146242120379 1107 34-35 0.7627302539394023 1107 36-37 0.404900629864251 1107 38-39 0.24612000291815406 1107 40-41 0.45477366284686127 1107 42-43 0.36404812827239397 1107 44-45 -0.07752607703579173 1107 46-47 0.22811756373258874 1107 48-49 0.7025607049490148 1107 50-51 0.37922320496787876 1107 52-53 0.31432655122917197 1107 54-55 0.5544321063552857 1107 56-57 0.41218933465694363 1107 58-59 0.31961400533447204 1107 60-61 0.53825288703176 1107 62-63 0.5594883557953736 1107 64-65 0.40542186777373956 1107 66-67 0.4536348491035085 1107 68-69 0.6074913060952554 1107 70-71 0.5217973607281081 1107 72-73 0.5190539081258052 1107 74-75 0.21318786289806013 1107 76 -0.5578288067456469 1108 1 -1.0049102530415865 1108 2 -0.34978251179095565 1108 3 -0.5078856145298474 1108 4 -0.21005211358176368 1108 5 -0.2888914556638582 1108 6 -0.21948387932329894 1108 7 0.21497173924826996 1108 8 -0.3629131381375146 1108 9 -0.5178731978088322 1108 10-11 -0.7584853869384389 1108 12-13 -0.9067476098078657 1108 14-15 -0.3656771812180324 1108 16-17 -0.5943742953576745 1108 18-19 -0.619417646923047 1108 20-21 -0.7314002513054021 1108 22-23 -0.7796392099413367 1108 24-25 -0.8316579175504586 1108 26-27 -0.8843757410180544 1108 28-29 -0.8870750852649749 1108 30-31 -0.7806509897617104 1108 32-33 -1.0244021303662834 1108 34-35 -0.691693974117257 1108 36-37 -0.6861546063118951 1108 38-39 -0.5610904812873798 1108 40-41 -0.5165192311257556 1108 42-43 -0.0889018509844064 1108 44-45 -0.6704815384238074 1108 46-47 -0.6955474632335665 1108 48-49 -0.6843592263020994 1108 50-51 -0.8190188089004309 1108 52-53 -0.4555609909980589 1108 54-55 -0.5369898399494559 1108 56-57 -0.38633123074339437 1108 58-59 -0.678048680613486 1108 60-61 -0.421549756244481 1108 62-63 -0.5867956482347267 1108 64-65 -0.8511603938276409 1108 66-67 -0.5412126883824158 1108 68-69 -0.8905825396938738 1108 70-71 -0.6618688515637174 1108 72-73 -0.866491786178706 1108 74-75 -0.8203642617982041 1108 76 -0.14611334417230637 1109 1 0.21829331604145708 1109 2 -0.4982149989013429 1109 3 -0.48179688567598333 1109 4 -0.29613887974190334 1109 5 -0.4236681237648021 1109 6 -0.0929052319212289 1109 7 0.040171216869786974 1109 8 -0.6657190352772631 1109 9 -0.17037072556962585 1109 10-11 -0.10561419248833204 1109 12-13 0.3146291443496523 1109 14-15 0.030160398327680582 1109 16-17 0.06307148522826367 1109 18-19 -0.07146752953885738 1109 20-21 -0.14964273456588728 1109 22-23 -0.037684366994582774 1109 24-25 -0.54102827030691 1109 26-27 -0.21494243108606526 1109 28-29 -0.2574693602383604 1109 30-31 0.14821646047267478 1109 32-33 -0.16914324351557752 1109 34-35 0.03549159857412576 1109 36-37 -0.052985837298294314 1109 38-39 0.40863874246724663 1109 40-41 0.24867466036747743 1109 42-43 0.12257462311180234 1109 44-45 0.04935148163340841 1109 46-47 -0.07304140530391123 1109 48-49 0.13244596124838637 1109 50-51 0.04657818968794203 1109 52-53 -0.22835277184066172 1109 54-55 0.3469168264152529 1109 56-57 -0.10067248279101193 1109 58-59 -0.15019730182115154 1109 60-61 0.23425415555508522 1109 62-63 0.2891054923922738 1109 64-65 0.06383232155911855 1109 66-67 0.2688664987604312 1109 68-69 -0.17907964472063753 1109 70-71 0.17920957314810693 1109 72-73 -0.03138070526288317 1109 74-75 -0.09716069271515693 1109 76 -0.08777604770741831 1110 1 -0.12461253237186654 1110 2 -0.3473713936910947 1110 3 -0.06349108971479822 1110 4 -0.5608473332780513 1110 5 -0.1944085731047771 1110 6 -0.43421566115455335 1110 7 -0.2464578590983919 1110 8 -0.8633324630244452 1110 9 0.3286239417964012 1110 10-11 0.029504699023163994 1110 12-13 0.10399010247535756 1110 14-15 0.09395531047034922 1110 16-17 -0.14510886270424805 1110 18-19 -0.02505831544672077 1110 20-21 0.023624159393378363 1110 22-23 0.17510739801984698 1110 24-25 0.32940402272569713 1110 26-27 0.16206823418188065 1110 28-29 -0.3121507135035131 1110 30-31 0.04724609593031914 1110 32-33 -0.06508038837089458 1110 34-35 -0.349944191267646 1110 36-37 0.1369127552360183 1110 38-39 -0.011415458074750973 1110 40-41 0.18875902088850438 1110 42-43 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0.33802686670202675 2116 3 0.5133872062923253 2116 4 0.14646723421732588 2116 5 0.10855012835402533 2116 6 0.4168733053545921 2116 7 1.129800616348497 2116 8 0.7176893847941983 2116 9 0.22726102144847715 2116 10-11 1.0063470385889488 2116 12-13 0.943264809687193 2116 14-15 0.7942326887321087 2116 16-17 0.5650565369259155 2116 18-19 1.1158652514322611 2116 20-21 0.8752610335800739 2116 22-23 0.8309785712271633 2116 24-25 0.7827034542714841 2116 26-27 1.2244012935613782 2116 28-29 1.1027010089812492 2116 30-31 0.9649400894924014 2116 32-33 0.948026590603142 2116 34-35 0.829033276029115 2116 36-37 0.44242265181843976 2116 38-39 0.78210895806113 2116 40-41 0.06117358153503005 2116 42-43 0.6805036100449868 2116 44-45 0.8811658188850586 2116 46-47 0.7099579215046461 2116 48-49 0.7191279904890564 2116 50-51 0.9052684007938794 2116 52-53 1.184473251324036 2116 54-55 0.9208858941314091 2116 56-57 0.715304594177212 2116 58-59 0.8761003178429405 2116 60-61 0.6550031241912677 2116 62-63 0.7501984791183247 2116 64-65 0.8786313134980901 2116 66-67 0.41376664202938684 2116 68-69 0.7266131383218379 2116 70-71 0.7398485871815623 2116 72-73 0.8892344881502012 2116 74-75 1.0454652717610529 2116 76 1.3670424495221383 2117 1 0.20815032072082928 2117 2 -0.08514064307739844 2117 3 0.18983671168892968 2117 4 -0.6355283829616951 2117 5 -0.292375588974501 2117 6 -0.6715613721803777 2117 7 0.1230318203954397 2117 8 -0.2576726075461053 2117 9 0.17120596994008963 2117 10-11 -0.011107443562323738 2117 12-13 -0.49003541354994695 2117 14-15 0.02915394131776594 2117 16-17 0.06328497610915917 2117 18-19 -0.2770679617489762 2117 20-21 -0.1999176585369895 2117 22-23 -0.1482900711223607 2117 24-25 -0.29713454519603033 2117 26-27 0.15388702891271322 2117 28-29 -0.4827588800145861 2117 30-31 0.1852331650448633 2117 32-33 -0.31679615091281477 2117 34-35 -0.4289643482361427 2117 36-37 -0.14650355704141305 2117 38-39 -0.11538116011119826 2117 40-41 -0.10369421701904713 2117 42-43 0.0970559274103735 2117 44-45 -0.07745338770146049 2117 46-47 0.24566562421011895 2117 48-49 0.3859772746965717 2117 50-51 0.5801921110361619 2117 52-53 0.10639657243734746 2117 54-55 0.06212434826539237 2117 56-57 -0.2807324345159401 2117 58-59 0.15336494601997686 2117 60-61 -0.03589023399170088 2117 62-63 -0.1256898613490378 2117 64-65 -0.2985401285319398 2117 66-67 0.30312089275132337 2117 68-69 -0.2796948470141416 2117 70-71 0.26251022930579637 2117 72-73 -1.2943012605134498 2117 74-75 -0.010840665527744875 2117 76 0.4849894870218847 2118 1 -0.374014168495183 2118 2 -0.29305424297484706 2118 3 -0.27411517430737575 2118 4 -0.36472687459195186 2118 5 -0.21364552519994362 2118 6 -0.04773511244594886 2118 7 -0.090981490006115 2118 8 0.210060885312636 2118 9 -0.7734372877967104 2118 10-11 -1.4693655542544377 2118 12-13 -0.3289752817278355 2118 14-15 -0.263589432858339 2118 16-17 -0.08960838044082209 2118 18-19 -0.4374337307291185 2118 20-21 -0.3838169866074068 2118 22-23 -0.24785102015612637 2118 24-25 -0.6356631369213588 2118 26-27 -0.050891255690586945 2118 28-29 -0.38402582513856487 2118 30-31 -0.3599271259465766 2118 32-33 -0.2337877437811784 2118 34-35 -0.4429317238650796 2118 36-37 -0.019403109444439792 2118 38-39 -0.3358894337115217 2118 40-41 -0.6061138044606977 2118 42-43 -0.3470478638107366 2118 44-45 -0.1711015700731835 2118 46-47 -0.8317184439015364 2118 48-49 -0.6861082708055619 2118 50-51 -0.7135393948766122 2118 52-53 -0.802973052927193 2118 54-55 -0.5082092663739104 2118 56-57 -0.25200183661611675 2118 58-59 0.00564726326901166 2118 60-61 -0.14597345070769308 2118 62-63 -0.42335252919790634 2118 64-65 -0.42846075751784696 2118 66-67 -0.5340082907847972 2118 68-69 -0.28944797431755376 2118 70-71 -0.010294248800413186 2118 72-73 -0.3422550266306814 2118 74-75 -0.3672090410391675 2118 76 -0.5555933504651627 2119 1 0.9038504657305992 2119 2 0.9253462955351317 2119 3 0.2672067808312697 2119 4 1.0074638621678673 2119 5 1.5241240012152737 2119 6 1.1891767065452186 2119 7 0.5765971693646108 2119 8 1.1014577541376909 2119 9 0.9696739101708474 2119 10-11 0.8827884856560928 2119 12-13 1.058133049976938 2119 14-15 1.7283112033651378 2119 16-17 1.126159321988986 2119 18-19 1.3025560636382423 2119 20-21 1.039700482395702 2119 22-23 0.9677755405456736 2119 24-25 1.5380009023377568 2119 26-27 1.3186937876290656 2119 28-29 1.3541996976286867 2119 30-31 0.8337736101618205 2119 32-33 1.3555658271045345 2119 34-35 0.8100811091751083 2119 36-37 1.4192762382636346 2119 38-39 1.1307414046703457 2119 40-41 1.2472399401379164 2119 42-43 0.9505277555843037 2119 44-45 1.1518144180274774 2119 46-47 0.8759064665277521 2119 48-49 0.7345103642453523 2119 50-51 1.3760856024091268 2119 52-53 1.3944353678054426 2119 54-55 1.2611922926993273 2119 56-57 1.0528425817714009 2119 58-59 1.0398632752552075 2119 60-61 0.811928314649613 2119 62-63 0.5604270291370526 2119 64-65 1.1359270130942747 2119 66-67 0.9608400361428693 2119 68-69 0.9409984678251959 2119 70-71 0.8709838438319863 2119 72-73 1.3210025244517638 2119 74-75 1.47891933279098 2119 76 1.1365982548591376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 18.0 13 224.0 14 1000.0 15 2033.0 16 2663.0 17 3047.0 18 3196.0 19 2785.0 20 2255.0 21 1732.0 22 1283.0 23 1020.0 24 1006.0 25 1034.0 26 1204.0 27 1316.0 28 1624.0 29 1819.0 30 2250.0 31 2794.0 32 3583.0 33 4547.0 34 6418.0 35 9489.0 36 17878.0 37 55143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.07493091556855 19.994518921140976 11.53538721538356 31.39516294790691 2 19.54385243717694 18.876987842662587 34.26131043460388 27.317849285556594 3 18.746812219760812 23.264895973690823 27.33155198270415 30.656739823844216 4 15.015111029909942 18.414902444408916 30.183996772253565 36.38598975342758 5 16.003989007391844 31.127199092576944 30.701654220050088 22.16715767998112 6 29.472217781533335 33.44599995432434 16.470641971361363 20.611140292780963 7 27.858344561932384 25.51366082779516 21.785004681754856 24.842989928517596 8 24.437999101712077 29.616096101582663 21.016892380539126 24.929012416166138 9 23.72317506718128 16.27499790653238 20.343176437450992 39.65865058883535 10-11 26.485791064318935 22.074283843758803 24.939670069503126 26.500255022419132 12-13 24.973641239185294 19.776873565493432 24.304109682895543 30.945375512425727 14-15 24.230174861640823 20.90993521669293 22.699279085877848 32.1606108357884 16-17 25.61224412116229 22.73505835065202 22.952017722154977 28.70067980603071 18-19 24.983442574280037 21.938779394188536 23.635249427151134 29.442528604380296 20-21 24.46768827886511 22.40124542291852 24.006364141564088 29.124702156652276 22-23 26.169106508019883 22.256605841916553 22.70689169540427 28.867395954659298 24-25 25.425354557288692 23.530576046162864 22.131759045683268 28.912310350865173 26-27 24.473017105533607 23.537427394736643 23.78141153005839 28.20814396967136 28-29 25.23275553627028 22.796720487816017 24.959082223795495 27.01144175211821 30-31 25.32258432868203 22.087225279953714 24.627172448443602 27.963017942920654 32-33 24.114082566362924 23.565974680460716 24.528589155076467 27.791353598099892 34-35 24.49433241220758 23.28392749750687 24.573503551282343 27.648236539003207 36-37 24.85478947328355 22.013382967547447 24.527447263647506 28.6043802955215 38-39 23.935947503444705 22.1123468913909 26.6901896301033 27.26151597506109 40-41 23.973629920600484 21.61410159788674 26.77697337870449 27.635295102808293 42-43 22.81194570686886 21.914799674180312 26.32288122045356 28.95037339849727 44-45 22.176673441889143 21.67614436552706 27.498268131332736 28.64891406125106 46-47 22.43588279626373 22.510486369622644 27.46325012751121 27.590380706602417 48-49 22.401626053394843 22.791391661147525 26.998500315923295 27.808481969534338 50-51 22.006531618973668 23.732310198612982 25.92854804698503 28.33261013542832 52-53 21.542923698814718 23.63030123095896 26.180906052785836 28.64586901744049 54-55 21.590121877878516 22.909387108807028 26.249800168999933 29.250690844314526 56-57 21.593166921689086 23.31209415275462 25.876782302205374 29.217956623350915 58-59 22.18733109522613 23.28392749750687 25.458088778252296 29.0706526290147 60-61 21.962759114196757 22.382594529578796 25.914464719361153 29.7401816368633 62-63 21.923934805612017 22.54816878677842 25.679235084994783 29.848661322614777 64-65 22.52951789343869 22.89911008594636 24.729181416097624 29.84219060451732 66-67 22.584414645193952 23.638003813931892 23.676828270294344 30.100753270579816 68-69 22.41494812006608 24.229032970211858 23.842312406269745 29.51370650345232 70-71 23.20247257557418 23.869717800564853 24.38813650931403 28.539673114546936 72-73 24.322668067386818 23.355486027055214 24.032247013953913 28.28959889160405 74-75 23.511925152823135 24.501945021734002 24.29906897785492 27.687060847587947 76 23.254999581306475 24.37709822550072 24.180692899719094 28.187209293473707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.0 15 3.5 16 6.0 17 7.0 18 10.0 19 14.5 20 19.0 21 22.0 22 39.0 23 77.0 24 86.0 25 74.0 26 177.5 27 315.5 28 422.0 29 494.0 30 606.0 31 859.5 32 1246.5 33 1492.0 34 1697.5 35 2161.0 36 2645.0 37 2871.0 38 3232.5 39 4121.0 40 5145.5 41 5740.0 42 5837.0 43 6053.5 44 6604.0 45 6898.5 46 6859.0 47 6860.5 48 6617.0 49 5987.0 50 5602.0 51 5289.5 52 4417.0 53 3596.5 54 3336.0 55 3281.0 56 2970.5 57 2597.5 58 2480.0 59 2450.5 60 2457.0 61 2498.0 62 2503.0 63 2617.5 64 2851.5 65 2962.0 66 3017.0 67 3081.0 68 3158.5 69 3292.0 70 3235.5 71 3123.0 72 3034.0 73 2697.0 74 2253.5 75 2058.0 76 1821.5 77 1339.0 78 882.0 79 671.0 80 499.0 81 236.5 82 107.5 83 69.0 84 45.0 85 17.0 86 13.5 87 14.0 88 12.0 89 7.5 90 4.5 91 3.0 92 2.0 93 1.0 94 2.5 95 5.0 96 5.0 97 5.5 98 4.5 99 16.0 100 29.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.8063047632097807E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.8063047632097807E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 131361.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.94655186851501 #Duplication Level Percentage of deduplicated Percentage of total 1 76.34024481638771 46.52674690357107 2 12.157132150886834 14.818705704128318 3 4.737696727454409 8.66238838011282 4 2.0859355483387456 5.085223163648267 5 1.2078441169123157 3.6806967060238582 6 0.7594304271796153 2.777079955237856 7 0.6007994004496628 2.563165627545466 8 0.4471646265301024 2.1802513683665623 9 0.32850362228328756 1.8019046749035101 >10 1.335248563577317 11.903837516462268 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009135131431703473 0.0 2 0.0 0.0 0.0 0.009135131431703473 0.0 3 0.0 0.0 0.0 0.009135131431703473 0.0 4 0.0 0.0 0.0 0.022837828579258684 0.0 5 0.0 0.0 0.0 0.03501800382152998 0.0 6 0.0 0.0 0.0 0.03501800382152998 0.0 7 0.0 0.0 0.0 0.03501800382152998 0.0 8 0.0 0.0 0.0 0.03730178667945585 0.0 9 0.0 0.0 0.0 0.039585569537381717 0.0 10 0.0 0.0 0.0 0.04491439620587541 0.0 11 0.0 0.0 0.0 0.04491439620587541 0.0 12 0.0 0.0 0.0 0.04491439620587541 0.0 13 0.0 0.0 0.0 0.046436918111159325 0.0 14 0.0 0.0 0.0 0.047198179063801284 0.0 15 0.0 0.0 0.0 0.04872070096908519 0.0 16 0.0 0.0 0.0 0.04948196192172715 0.0 17 0.0 0.0 0.0 0.0502432228743691 0.0 18 0.0 0.0 0.0 0.05633331049550475 0.0 19 0.0 0.0 0.0 0.062423398116640404 0.0 20 0.0 0.0 0.0 0.0677522247851341 0.0 21 0.0 0.0 0.0 0.07079726859570191 0.0 22 0.0 0.0 0.0 0.07840987812212148 0.0 23 0.0 0.0 0.0 0.08221618288533127 0.0 24 0.0 0.0 0.0 0.08754500955382495 0.0 25 0.0 0.0 0.0 0.09439635812760257 0.0 26 0.0 0.0 0.0 0.11342788194365147 0.0 27 0.0 0.0 0.0 0.16976119243915622 0.0 28 0.0 0.0 0.0 0.24664854865599378 0.0 29 0.0 0.0 0.0 0.34104490678359634 0.0 30 0.0 0.0 0.0 0.42097730681100176 0.0 31 0.0 0.0 0.0 0.5130898820806784 0.0 32 0.0 0.0 0.0 0.6341303735507494 0.0 33 0.0 0.0 0.0 0.8267293945691644 0.0 34 0.0 0.0 0.0 1.043688766072122 0.0 35 0.0 0.0 0.0 1.3588508004658917 0.0 36 0.0 0.0 0.0 1.7950533263297326 0.0 37 0.0 0.0 0.0 2.345444995089867 0.0 38 0.0 0.0 0.0 2.8714763133654584 0.0 39 0.0 0.0 0.0 3.4112103287886053 0.0 40 0.0 0.0 0.0 3.9836785651753566 0.0 41 0.0 0.0 0.0 4.58050715204665 0.0 42 0.0 0.0 0.0 5.143840257001697 0.0 43 0.0 0.0 0.0 5.7848219791262245 0.0 44 0.0 0.0 0.0 6.446357746972085 0.0 45 0.0 0.0 0.0 7.1162673852970055 0.0 46 0.0 0.0 0.0 7.667420315009783 0.0 47 0.0 0.0 0.0 8.279474120933916 0.0 48 0.0 0.0 0.0 8.810073004925359 0.0 49 0.0 0.0 0.0 9.307937667953198 0.0 50 0.0 0.0 0.0 9.834730247181431 0.0 51 0.0 0.0 0.0 10.396540830231196 0.0 52 0.0 0.0 0.0 10.962918978996811 0.0 53 0.0 0.0 0.0 11.507981821088451 0.0 54 0.0 0.0 0.0 11.99823387458987 0.0 55 0.0 0.0 0.0 12.479350796659586 0.0 56 0.0 0.0 0.0 12.995485722550834 0.0 57 0.0 0.0 0.0 13.481931471289043 0.0 58 0.0 0.0 0.0 13.931075433347797 0.0 59 0.0 0.0 0.0 14.38326443921712 0.0 60 0.0 0.0 0.0 14.834692184133798 0.0 61 0.0 0.0 0.0 15.329511803351071 0.0 62 0.0 0.0 0.0 15.854020599721379 0.0 63 0.0 0.0 0.0 16.405173529434155 0.0 64 0.0 0.0 0.0 16.922069716278042 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATCA 20 7.902541E-5 70.00001 35 CCCATTG 15 0.002220508 70.0 24 GAGTTAG 20 0.006938404 52.500004 1 GAACCGT 20 0.006938404 52.500004 32 TAGGATC 35 0.001251713 40.0 24 TCCCGAA 40 0.0024116663 35.000004 32 CCGAATC 40 0.0024116663 35.000004 34 GGACAGC 40 0.0024116663 35.000004 6 CCCGAAT 40 0.0024116663 35.000004 33 TCATAAA 45 0.0042947163 31.111109 59 GGATCCT 60 4.9605814E-4 29.166666 26 CGTATCA 50 0.007187505 27.999998 57 CTCCCGA 50 0.007187505 27.999998 31 GAATCAA 50 0.007187505 27.999998 36 TTCATAA 65 7.921658E-4 26.923079 58 ATATCTC 75 0.0018244415 23.333334 40 GGATTAG 75 0.0018244415 23.333334 1 ACGACGG 90 1.9162487E-4 23.333332 5 TATATCT 115 4.0839066E-5 21.304348 39 ATGATAT 90 0.005252512 19.444443 18 >>END_MODULE