FastQCFastQC Report
Wed 25 May 2016
SRR1780326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131361
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT91216.943461149047282No Hit
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG23751.8079947625246457RNA PCR Primer, Index 23 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG23381.7798281072768936No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC23091.7577515396502768No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC5760.43848630872176675No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT3040.23142332960315468No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2930.22304945912409316RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1980.15072966862310733No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1670.12713057909120667No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1670.12713057909120667No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTA207.902541E-570.0000111
TGACATA207.902541E-570.0000146
TTTCTTA207.902541E-570.0000169
CAGACTT207.902541E-570.0000110
GGAATCG150.00222050870.058
CAGGTTA252.3832214E-455.99999664
AGTGGCA252.3832214E-455.99999659
GGATAAA252.3832214E-455.9999961
CCAGACT252.3832214E-455.9999969
GACTTAG252.3832214E-455.99999612
GGCCAGA252.3832214E-455.9999967
GATGACA252.3832214E-455.99999644
GACATAA252.3832214E-455.99999647
TAGTGGC252.3832214E-455.99999658
CTTAATA200.00693840452.5000042
TTAATAC200.00693840452.5000043
GAAAGCA200.00693840452.50000433
GCTTATA200.00693840452.5000043
TAACACG200.00693840452.50000425
CTGCACT200.00693840452.50000425
Produced by FastQC (version 0.11.5)