FastQCFastQC Report
Wed 25 May 2016
SRR1780325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504718
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAT353.1785652E-431.49006716
TTGACAC300.005684513629.3936518
AGGTAAC300.005706648329.37028940
GTTTACA300.005784655529.288821
CTTATAA300.005784655529.288821
ATTGCGA406.946563E-427.55380815
GATTTCG407.0726575E-427.46915441
CGATTAG450.00138046124.49470919
CATTGCG450.001386068924.47767314
CCAGTTG450.001386068924.47767314
GGTACAA450.001403815624.42428630
TTCTAAG450.001409501424.4073492
ATTAGAG450.001409501424.4073493
CCATATT450.001409501424.4073492
GTTGTAA551.6083058E-423.9635811
AACATTG651.8264702E-523.6657342
ACATTGT651.8292745E-523.66104143
CCATTGC500.002562539722.0233413
ATGCCAT500.002578842221.99930610
GTTACTG703.238499E-521.97532331