FastQCFastQC Report
Wed 25 May 2016
SRR1780325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504718
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT230024.557396407498841No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC62581.2399003007620095No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG61091.210378865029581No Hit
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG39900.7905404602173887Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC13030.2581639648278841No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT5220.10342409028407942Illumina PCR Primer Index 2 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATGG207.8546314E-443.99732635
TCTTCTG5000.037.39772444
TCTTTAT1850.035.747881
GTCTTCT5400.035.03490443
CGTCTTC5450.034.71348642
CTTTATA1850.034.4912222
CCGTCTT5600.034.17649541
ATGCCGT31700.033.37967744
TTTCGTC401.8187393E-532.99799340
CCTTATA300.00573802929.3373662
ACGTGTA300.00573802929.3373662
GAATAAG300.005743589329.3315536
GATAGTC300.005743589329.3315526
TATGCCG37200.028.44450643
CTTAGGC554.948246E-628.0038453
TACAAGG953.092282E-1127.7932932
GCCGTCT7150.026.45992940
GTACAAG756.859773E-826.453431
TTTATAC2850.026.2492183
TGTATAA858.216375E-925.88078111
Produced by FastQC (version 0.11.5)