FastQCFastQC Report
Wed 25 May 2016
SRR1780324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156827
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAATC200.00694090152.5000047
TCGATTC200.00694090152.5000044
CCGAAAT200.00694090152.50000447
GCATGTA200.00694090152.5000042
GACGAAT404.5765857E-543.75000423
ACGAATT404.5765857E-543.75000424
CTATCGG350.001252455440.00000442
ATAAGAG459.16559E-538.888893
GAGTGAC400.002413088235.00000425
AGGAGTG400.002413088235.0000045
AAGAGCA400.002413088235.00000416
TTCCCAG400.002413088235.00000438
AGTGACT400.002413088235.00000426
TACAGTA450.004297231331.11111354
TAGATCT802.4507062E-630.62500239
GTAGATC703.4718512E-530.00000238
ACAGTAG500.00719169227.99999855
TTGTCTG500.00719169227.9999989
CGGTGTA500.00719169227.99999834
GGAGTGA500.00719169227.9999986