##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780324_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156827 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.32186421980909 34.0 31.0 34.0 12.0 34.0 2 30.492064504198893 34.0 32.0 34.0 12.0 34.0 3 30.299941974277388 34.0 32.0 34.0 11.0 34.0 4 30.1904455227735 34.0 31.0 34.0 11.0 34.0 5 30.129078538772024 34.0 31.0 34.0 11.0 34.0 6 33.21723937842336 38.0 34.0 38.0 11.0 38.0 7 33.52056087280889 38.0 34.0 38.0 21.0 38.0 8 33.48806009169339 38.0 34.0 38.0 21.0 38.0 9 33.737819380591354 38.0 34.0 38.0 21.0 38.0 10-11 33.464518227091 38.0 34.0 38.0 21.0 38.0 12-13 33.543232351572115 38.0 34.0 38.0 21.0 38.0 14-15 33.45000223175856 38.0 34.0 38.0 16.0 38.0 16-17 33.53563799600834 38.0 34.0 38.0 16.0 38.0 18-19 33.53473891613051 38.0 34.0 38.0 21.0 38.0 20-21 33.5382937886971 38.0 34.0 38.0 21.0 38.0 22-23 33.553944792669625 38.0 34.0 38.0 21.0 38.0 24-25 33.587095971994614 38.0 34.0 38.0 21.0 38.0 26-27 33.563142826171514 38.0 34.0 38.0 21.0 38.0 28-29 33.64165609238205 38.0 34.0 38.0 21.0 38.0 30-31 33.642478654823464 38.0 34.0 38.0 21.0 38.0 32-33 33.72108756782952 38.0 34.0 38.0 21.0 38.0 34-35 33.67891051923457 38.0 34.0 38.0 21.0 38.0 36-37 33.618426673978334 38.0 34.0 38.0 21.0 38.0 38-39 33.61112563525413 38.0 34.0 38.0 21.0 38.0 40-41 33.67434179063554 38.0 34.0 38.0 21.0 38.0 42-43 33.71667825055634 38.0 34.0 38.0 21.0 38.0 44-45 33.795207457899465 38.0 34.0 38.0 21.0 38.0 46-47 33.72723446855452 38.0 34.5 38.0 21.0 38.0 48-49 33.67100690569863 38.0 34.0 38.0 21.0 38.0 50-51 33.59625893500481 38.0 34.0 38.0 21.0 38.0 52-53 33.667247986634955 38.0 34.0 38.0 21.0 38.0 54-55 33.64847252067565 38.0 34.0 38.0 21.0 38.0 56-57 33.596836004004416 38.0 34.0 38.0 21.0 38.0 58-59 33.65900960931472 38.0 34.0 38.0 21.0 38.0 60-61 33.67462235456905 38.0 34.0 38.0 20.0 38.0 62-63 33.557831240793995 38.0 34.0 38.0 19.5 38.0 64-65 33.50498638627277 38.0 34.0 38.0 19.0 38.0 66-67 33.388067743437034 38.0 34.0 38.0 19.0 38.0 68-69 33.33229928519961 38.0 34.0 38.0 19.0 38.0 70-71 33.27689747301166 38.0 34.0 38.0 11.0 38.0 72-73 33.21950301925051 38.0 34.0 38.0 11.0 38.0 74-75 33.24758491841328 38.0 34.0 38.0 11.0 38.0 76 31.719098114482836 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3569160454884788 1101 2 0.6853366998254806 1101 3 0.3918247172275713 1101 4 0.6893367633626575 1101 5 0.7566692756832261 1101 6 0.25717174508968554 1101 7 0.08594338983532879 1101 8 -0.30258466395677175 1101 9 0.09271833519863293 1101 10-11 0.36877268369431704 1101 12-13 0.429052065794032 1101 14-15 0.39575201404571914 1101 16-17 0.25234787570752815 1101 18-19 0.26190796083788825 1101 20-21 0.3872560803291023 1101 22-23 0.30229716218356373 1101 24-25 0.31070707793672625 1101 26-27 0.3305298420639602 1101 28-29 0.323341907050704 1101 30-31 0.33669198546768797 1101 32-33 0.19569923272040768 1101 34-35 0.304139041731176 1101 36-37 0.1912710507968356 1101 38-39 0.40064750290532203 1101 40-41 0.3432147280841562 1101 42-43 0.35893196761649193 1101 44-45 0.5737700372749046 1101 46-47 0.3233466484477816 1101 48-49 0.5113836919619885 1101 50-51 0.682765708042318 1101 52-53 0.34992857153892487 1101 54-55 0.5105426561198598 1101 56-57 0.6559199414206276 1101 58-59 0.3723120275735283 1101 60-61 0.5593120868196308 1101 62-63 0.5302268290819683 1101 64-65 0.6649821309843702 1101 66-67 0.1322640614959596 1101 68-69 -0.08555072546286624 1101 70-71 0.20553812814082306 1101 72-73 0.20801163810686063 1101 74-75 -0.007495549172112703 1101 76 -0.7898802868035126 1102 1 0.09357152727139706 1102 2 -0.12015341226133458 1102 3 0.28548502992836333 1102 4 0.28942957410014714 1102 5 -0.15990814988970214 1102 6 0.3585377287645173 1102 7 -0.03355638408723394 1102 8 0.2766019332208316 1102 9 0.26423971736468843 1102 10-11 0.36616684375714215 1102 12-13 0.3049712490595411 1102 14-15 0.6307833079225844 1102 16-17 0.5139551672998266 1102 18-19 0.13718460822848755 1102 20-21 0.45976149428879154 1102 22-23 0.19885483572774376 1102 24-25 0.23724975897715694 1102 26-27 -0.12441530441042659 1102 28-29 0.12917113529373125 1102 30-31 0.5085584331962067 1102 32-33 0.30462691173804757 1102 34-35 0.1725798213413725 1102 36-37 0.3250196526863647 1102 38-39 0.5859834063821481 1102 40-41 0.18148606860437155 1102 42-43 0.24107422981872162 1102 44-45 0.3516370561464157 1102 46-47 -0.09785941518706665 1102 48-49 0.20572699650019644 1102 50-51 0.21215577491364712 1102 52-53 0.17720540931051687 1102 54-55 0.2078190179389523 1102 56-57 0.19995149707137472 1102 58-59 -0.09554927986798134 1102 60-61 0.3521775588455185 1102 62-63 0.20145669034181424 1102 64-65 0.35250146655467773 1102 66-67 0.5357408945080238 1102 68-69 0.13428578485341092 1102 70-71 0.09232687662372285 1102 72-73 0.47742455069818135 1102 74-75 -0.0454230140111207 1102 76 -0.5080569127762402 1103 1 0.6092200028741068 1103 2 0.6716779447874153 1103 3 0.5005880047567395 1103 4 0.33564689606117426 1103 5 0.5803664496526331 1103 6 0.21039728542956482 1103 7 0.31869611805085185 1103 8 0.14879063950564841 1103 9 0.2913182833448573 1103 10-11 0.18069080187378006 1103 12-13 0.15380281413825259 1103 14-15 -0.0810316378740481 1103 16-17 0.036936698862596984 1103 18-19 0.36010604224831866 1103 20-21 0.061361013508445694 1103 22-23 -0.17494610271850064 1103 24-25 0.28470948206617663 1103 26-27 -0.08279775592789207 1103 28-29 0.45891595188064827 1103 30-31 -0.18429111491650474 1103 32-33 0.5052544687729252 1103 34-35 0.2552197214869949 1103 36-37 0.3056439736997021 1103 38-39 -0.20319556872359357 1103 40-41 0.2775843512015044 1103 42-43 0.14436341457243884 1103 44-45 0.33646242655531466 1103 46-47 0.6042821376409862 1103 48-49 0.8814487448764012 1103 50-51 -0.0797911543330585 1103 52-53 0.5490647348311626 1103 54-55 0.29824270561664434 1103 56-57 0.5209763913228116 1103 58-59 0.37580037225969676 1103 60-61 0.6319662928676593 1103 62-63 0.2696616229632127 1103 64-65 0.31139243574313014 1103 66-67 0.5486734234588582 1103 68-69 0.3995413149830753 1103 70-71 0.3094754793558465 1103 72-73 0.3606561804973154 1103 74-75 0.5731193894283066 1103 76 1.2280917592992076 1104 1 -0.34021039129313024 1104 2 -0.24282421971475188 1104 3 -0.5719683862207035 1104 4 0.22390162378958678 1104 5 -0.255974402477694 1104 6 -0.10309098069028266 1104 7 0.1577824702950963 1104 8 -0.7630115933492121 1104 9 -0.0822406138982501 1104 10-11 -0.07560334257826185 1104 12-13 -0.36713020021581855 1104 14-15 -0.22054519449287113 1104 16-17 -0.14280811772432855 1104 18-19 -0.2199064890800031 1104 20-21 -0.030231604399965306 1104 22-23 -0.35977180333367187 1104 24-25 0.16107653606480454 1104 26-27 0.14366613106231085 1104 28-29 -0.47201934089675035 1104 30-31 -0.20612410648633528 1104 32-33 -0.6095552828789366 1104 34-35 -0.30821728010790395 1104 36-37 0.02645167477056276 1104 38-39 0.07749479354571776 1104 40-41 -0.29648149523276146 1104 42-43 -0.30092024913227533 1104 44-45 -0.37220697421935256 1104 46-47 -0.08121000179852444 1104 48-49 -0.4527788696143702 1104 50-51 -0.5010602361284597 1104 52-53 -0.12778078806698545 1104 54-55 -0.28219133402792096 1104 56-57 -0.11723276793897952 1104 58-59 -0.21927253728689777 1104 60-61 -0.14323306917380307 1104 62-63 -0.10922308881622911 1104 64-65 -0.18982082468065187 1104 66-67 -0.3080479253677524 1104 68-69 -0.7072256023101531 1104 70-71 -0.22873937596426686 1104 72-73 -0.0320129924350141 1104 74-75 -0.31250375935273667 1104 76 -0.2852825856540875 1105 1 -0.2405745369513923 1105 2 -0.12907071831418904 1105 3 -0.16331292403582864 1105 4 0.30913971902768367 1105 5 0.21702279640186006 1105 6 0.32492022379150853 1105 7 -0.10375814595140298 1105 8 0.016204092925292457 1105 9 -0.06075602006071534 1105 10-11 0.2242145845926089 1105 12-13 -0.6674243178628743 1105 14-15 0.21288617805615218 1105 16-17 -0.14888654909687205 1105 18-19 -0.027231419051993555 1105 20-21 0.24397007627230494 1105 22-23 0.23518617985959622 1105 24-25 -0.0576209429267891 1105 26-27 0.0052067473088115435 1105 28-29 0.11662211568588532 1105 30-31 0.0760747730654856 1105 32-33 0.11520662188296882 1105 34-35 0.1922729159705341 1105 36-37 0.10996708093303198 1105 38-39 0.2611922725373077 1105 40-41 0.2290507176523917 1105 42-43 0.3930293306996546 1105 44-45 0.6117706168170614 1105 46-47 0.4679916788737444 1105 48-49 -0.0034091217152152353 1105 50-51 0.23370166863345077 1105 52-53 0.4032696321010789 1105 54-55 0.3047414390813259 1105 56-57 -0.10765293600620396 1105 58-59 0.2610776027691273 1105 60-61 0.42715908768894906 1105 62-63 0.3350486198672371 1105 64-65 0.3401371585126185 1105 66-67 0.31248428751739965 1105 68-69 0.24848868340413333 1105 70-71 0.2561205680133227 1105 72-73 0.39660045294314017 1105 74-75 0.1657875571738785 1105 76 0.29006755440193643 1106 1 -0.354694991815915 1106 2 0.179922683342415 1106 3 0.15754064889692287 1106 4 0.5946767118677769 1106 5 -0.4951265788613739 1106 6 0.2812026890449175 1106 7 -0.18916036411790316 1106 8 0.15003534380273464 1106 9 -0.10712412338796184 1106 10-11 -0.19537547577521508 1106 12-13 0.4482039286624584 1106 14-15 0.17243412133846903 1106 16-17 0.2231027812414652 1106 18-19 0.1660663487424756 1106 20-21 0.5375317778025632 1106 22-23 0.07251595663905164 1106 24-25 0.19470251628982282 1106 26-27 0.11542996786194948 1106 28-29 0.2978266643311187 1106 30-31 0.26598351938024223 1106 32-33 0.4584524452719947 1106 34-35 0.14705320517451526 1106 36-37 0.3966215804416606 1106 38-39 0.36170890878607764 1106 40-41 0.3329451441580815 1106 42-43 0.30126181701160704 1106 44-45 0.5570577149498703 1106 46-47 0.26250803434194836 1106 48-49 0.2109528044395006 1106 50-51 0.04797374500576268 1106 52-53 -0.053888868530677314 1106 54-55 0.16723757834848385 1106 56-57 0.2655493663944668 1106 58-59 -0.03581134851544476 1106 60-61 0.07757043122397533 1106 62-63 0.0791704786346159 1106 64-65 0.46233567037560164 1106 66-67 0.1085505517319163 1106 68-69 0.18314262135162807 1106 70-71 0.26048951368228046 1106 72-73 0.22351888618755567 1106 74-75 0.055861964024920496 1106 76 0.6390497248357399 1107 1 0.17811259007332936 1107 2 -0.1974480982516731 1107 3 0.49079903959020044 1107 4 -0.21057042589985286 1107 5 0.12270054576247347 1107 6 -0.170447778481865 1107 7 0.05823105552629215 1107 8 0.16549082210972443 1107 9 0.16762135987676885 1107 10-11 0.19466925921607725 1107 12-13 0.3653577224894988 1107 14-15 0.3119427282124434 1107 16-17 0.5103319788940297 1107 18-19 0.4439478932526413 1107 20-21 0.5165247793129382 1107 22-23 0.8655215023944152 1107 24-25 0.6370082130834334 1107 26-27 0.3067441158794324 1107 28-29 0.37675567635652385 1107 30-31 0.5484135056599726 1107 32-33 0.30341918226944387 1107 34-35 0.6266861015346095 1107 36-37 0.5979665125897498 1107 38-39 0.11496891362853034 1107 40-41 0.3928387256092023 1107 42-43 0.07440756315205732 1107 44-45 0.32748246677443404 1107 46-47 0.6944111162138995 1107 48-49 0.7262583974663741 1107 50-51 0.21215577491364712 1107 52-53 0.002084636363655079 1107 54-55 0.3624083899196293 1107 56-57 0.25429932315832815 1107 58-59 0.2655618312431258 1107 60-61 0.19034181005324768 1107 62-63 0.5130508932403615 1107 64-65 0.4454966356368004 1107 66-67 0.3425041795321775 1107 68-69 0.3867012437906041 1107 70-71 0.35802735971551414 1107 72-73 0.40737624151943663 1107 74-75 0.41230645458791315 1107 76 0.3590928456778677 1108 1 -0.5548166183993573 1108 2 -0.5660842929748249 1108 3 -0.7314464081987246 1108 4 -0.3568218194338577 1108 5 -0.15457788233117142 1108 6 -0.13656167666098185 1108 7 -0.5765352952726701 1108 8 -0.478716721554342 1108 9 -0.6993193684770063 1108 10-11 -0.7216083792082983 1108 12-13 -0.7926109694465922 1108 14-15 -0.8356092970569691 1108 16-17 -0.8585132459294513 1108 18-19 -0.7766601520836645 1108 20-21 -1.0661175934109508 1108 22-23 -0.8453143857512586 1108 24-25 -1.0315745628362976 1108 26-27 -0.8246603341391534 1108 28-29 -0.8427839929114072 1108 30-31 -1.0865270368892652 1108 32-33 -0.8295529935016432 1108 34-35 -0.8658294595950835 1108 36-37 -0.7333926475737584 1108 38-39 -1.3940367998608778 1108 40-41 -0.6308107076869689 1108 42-43 -0.6320328864949118 1108 44-45 -1.3735348130471934 1108 46-47 -0.8154132985018165 1108 48-49 -1.0587541443396447 1108 50-51 -0.46336563905885697 1108 52-53 -1.2423412276274277 1108 54-55 -0.8729536966652844 1108 56-57 -1.320254745960959 1108 58-59 -0.7445587094480715 1108 60-61 -0.525284351225082 1108 62-63 -1.0220893891825256 1108 64-65 -0.945796099405932 1108 66-67 -0.6777136762835028 1108 68-69 -0.6188670858266434 1108 70-71 -0.859874907099794 1108 72-73 -0.859485519907544 1108 74-75 -0.615674546898525 1108 76 -0.8064822193537218 1109 1 0.08768890239563731 1109 2 -0.21109717482980983 1109 3 -0.4372110383054597 1109 4 -0.3868411422163369 1109 5 -0.09905938766794264 1109 6 0.05489489308506279 1109 7 -0.2793900063510648 1109 8 -0.33924763800631297 1109 9 -0.12734200373648008 1109 10-11 0.2548534812996195 1109 12-13 -0.21102972174691814 1109 14-15 0.27662386451829235 1109 16-17 -0.35899631549146704 1109 18-19 -0.41501357497656244 1109 20-21 -0.10610310132408785 1109 22-23 0.11333140924321583 1109 24-25 -0.6148120490343132 1109 26-27 0.09782425364148395 1109 28-29 -0.3855816557748568 1109 30-31 -0.0790279570217649 1109 32-33 -0.07608524414573736 1109 34-35 -0.001957312005828271 1109 36-37 -0.4036861698409666 1109 38-39 -0.37185585580493097 1109 40-41 -0.3109323952099743 1109 42-43 -0.16424902561895038 1109 44-45 -0.2460734586121518 1109 46-47 -0.29284704896188885 1109 48-49 -0.3338622599082868 1109 50-51 0.16781650116933378 1109 52-53 -0.29662904507313925 1109 54-55 -0.042666385292442044 1109 56-57 -0.2385929866676193 1109 58-59 -0.16367331195083068 1109 60-61 -0.06420595593615985 1109 62-63 -0.2710082334961115 1109 64-65 -0.03669368865878653 1109 66-67 -0.0398433229526276 1109 68-69 -0.16026729745711066 1109 70-71 -0.06014174552453255 1109 72-73 -0.5328844893591409 1109 74-75 -0.058459557940118145 1109 76 -0.4226259440500755 1110 1 -0.5151819110014912 1110 2 -0.718274208322633 1110 3 -0.34630194739636977 1110 4 -0.5471282235560508 1110 5 -0.5081502967588492 1110 6 -0.033621853326415874 1110 7 -0.36312206376732803 1110 8 -0.7028840016426798 1110 9 -0.11538028124844146 1110 10-11 -0.5776824038678541 1110 12-13 -0.0842354633737088 1110 14-15 0.033800898011122626 1110 16-17 -0.3948814829555829 1110 18-19 -0.38271692425885817 1110 20-21 -0.25921314917098215 1110 22-23 -0.1570035189864427 1110 24-25 -0.29064936960847376 1110 26-27 -0.13064464588004654 1110 28-29 -0.21811754094231617 1110 30-31 -0.3948174307059702 1110 32-33 -0.5102957704615321 1110 34-35 -0.4636798000441047 1110 36-37 -0.2230641602715906 1110 38-39 0.13890969443429668 1110 40-41 -0.3946644526262375 1110 42-43 -0.19295715959023596 1110 44-45 0.03741936435435633 1110 46-47 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0.59772245739547 2116 16-17 0.6439734914710726 2116 18-19 0.3604301283255751 2116 20-21 0.5621329276197429 2116 22-23 0.6976173592279054 2116 24-25 0.43139673310913906 2116 26-27 0.5694460982215901 2116 28-29 0.30856358192755806 2116 30-31 0.847775729310051 2116 32-33 0.6328913509470127 2116 34-35 0.9051242634060515 2116 36-37 0.2787094744421523 2116 38-39 0.5065588329545818 2116 40-41 -0.013928129058704997 2116 42-43 0.40942457845392966 2116 44-45 0.2658882638758868 2116 46-47 0.15709049081232251 2116 48-49 0.2626152190555828 2116 50-51 0.4857213730669443 2116 52-53 0.6368743843468039 2116 54-55 0.7001978285336534 2116 56-57 0.7002549167900796 2116 58-59 0.7836420768346102 2116 60-61 0.35734164346987285 2116 62-63 0.38736968622481527 2116 64-65 0.06645996349668337 2116 66-67 0.3453182486880806 2116 68-69 0.7050896686258028 2116 70-71 0.7043066010472288 2116 72-73 0.24988355928912398 2116 74-75 0.6562975542761649 2116 76 1.153469466891071 2117 1 -0.06616277224551226 2117 2 -0.5612766006671315 2117 3 -0.12875410050641634 2117 4 0.2616397190276807 2117 5 -0.13371249771578775 2117 6 -0.18213859973790392 2117 7 0.27433008934271896 2117 8 0.007821739984116505 2117 9 -0.6885501377077716 2117 10-11 -0.15482953305445335 2117 12-13 -0.31088020021581997 2117 14-15 -0.3676285278261986 2117 16-17 -0.31697478439099314 2117 18-19 -0.17252553669905524 2117 20-21 -0.43558874725710695 2117 22-23 -0.5383432319051025 2117 24-25 -0.5157091782209093 2117 26-27 -0.5353814879853047 2117 28-29 -0.6549955313729399 2117 30-31 -0.4579693445815707 2117 32-33 -0.4810433781170289 2117 34-35 -0.4453006134412334 2117 36-37 -0.3922388014199072 2117 38-39 -0.44696949216857007 2117 40-41 -0.9562433999946691 2117 42-43 -0.8627059634179943 2117 44-45 -0.7091117361241146 2117 46-47 -0.644067144655665 2117 48-49 -0.39010029818579994 2117 50-51 -0.5587983313665532 2117 52-53 -0.48724507378127413 2117 54-55 -0.2816556197422031 2117 56-57 -0.5091970536532671 2117 58-59 -0.5907606325249901 2117 60-61 -0.9661497358404745 2117 62-63 -0.45612785072098916 2117 64-65 -0.5983922532520864 2117 66-67 -0.3278098301296595 2117 68-69 -0.8165113165958715 2117 70-71 -0.5856441378690249 2117 72-73 -1.6436201352921564 2117 74-75 -0.5252418545908313 2117 76 -0.9505206808921827 2118 1 0.6987620397845653 2118 2 -0.12064376858693038 2118 3 0.34770579924295575 2118 4 -0.20103571956880728 2118 5 -0.22414482854285467 2118 6 0.33650801680344955 2118 7 0.3589102898439691 2118 8 0.2668129680542961 2118 9 0.025654122943855384 2118 10-11 -0.7381973275406679 2118 12-13 -0.1487718042258379 2118 14-15 -0.23852765063321613 2118 16-17 0.21345754643607506 2118 18-19 0.2266473956317725 2118 20-21 0.32377842066269835 2118 22-23 -0.01841841987503301 2118 24-25 -0.17257008047654665 2118 26-27 0.14504457717760033 2118 28-29 0.045211235544357464 2118 30-31 0.08855007897732747 2118 32-33 0.14332692263484859 2118 34-35 -0.1680543728397339 2118 36-37 0.2034566873018946 2118 38-39 -0.4192470610908785 2118 40-41 0.09656361754920084 2118 42-43 -0.21044405865608695 2118 44-45 -0.17772075868050052 2118 46-47 -0.02513230756292728 2118 48-49 0.038887797052296946 2118 50-51 -0.17161161457456586 2118 52-53 0.3212793623089567 2118 54-55 0.07376104692446006 2118 56-57 0.1135942997301953 2118 58-59 -0.26745549468037666 2118 60-61 0.009273196490354962 2118 62-63 -0.20151632189893576 2118 64-65 -0.1413872407207606 2118 66-67 -0.17859930381386135 2118 68-69 0.04929068841666151 2118 70-71 0.055032553860293376 2118 72-73 0.23847572936949746 2118 74-75 -0.0424536340394539 2118 76 0.5573239306366382 2119 1 0.34670323271726744 2119 2 0.12001992538746009 2119 3 -0.09481489455108161 2119 4 0.4076012574892225 2119 5 0.9289363856589006 2119 6 0.49076462606854676 2119 7 0.43253728537249003 2119 8 0.6976170253439165 2119 9 1.0685156191160488 2119 10-11 0.7081760500720975 2119 12-13 0.5426092538784744 2119 14-15 0.3221140280050605 2119 16-17 0.7390363818621069 2119 18-19 0.3515191283133561 2119 20-21 0.4205837093185778 2119 22-23 0.36897686735048296 2119 24-25 0.551939705153778 2119 26-27 0.45848947975662924 2119 28-29 0.38930347607123394 2119 30-31 0.08375832291222451 2119 32-33 0.3058765970690729 2119 34-35 -0.0020344844089805747 2119 36-37 0.1845664094982027 2119 38-39 0.39748770386120214 2119 40-41 0.5221436967795299 2119 42-43 0.4828089249194818 2119 44-45 0.14802225891310883 2119 46-47 0.2259948900837898 2119 48-49 0.4200765008216436 2119 50-51 0.5517165569709164 2119 52-53 0.22540070785643707 2119 54-55 0.2408412785208256 2119 56-57 0.2513953781085192 2119 58-59 0.25912460320702024 2119 60-61 0.16516229889899137 2119 62-63 0.07794162818719741 2119 64-65 0.2923397566734707 2119 66-67 -0.09730114526612965 2119 68-69 -0.33354605604996124 2119 70-71 -0.1300361974223776 2119 72-73 0.3703531897698795 2119 74-75 -0.17511158163797802 2119 76 0.3400748526065769 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 27.0 13 357.0 14 1189.0 15 2125.0 16 2732.0 17 3137.0 18 3296.0 19 3091.0 20 2320.0 21 1816.0 22 1388.0 23 1225.0 24 1095.0 25 1095.0 26 1307.0 27 1456.0 28 1686.0 29 2075.0 30 2606.0 31 3064.0 32 4144.0 33 5246.0 34 7320.0 35 10974.0 36 20672.0 37 71383.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.06487403317031 19.384417224074937 11.54711879969648 31.003589943058273 2 19.48516518201585 19.172718983338328 34.077677950863055 27.264437883782765 3 20.041829531904582 21.154520586378624 27.78794467789347 31.015705203823323 4 15.42081401799435 17.884675470422824 32.07610934341663 34.618401168166194 5 16.324357412945474 31.15662481587992 33.274882513852845 19.244135257321762 6 30.186128664069322 33.033214943855334 17.19729383333227 19.583362558743072 7 28.000280563933504 27.87020092203511 20.34981221345814 23.779706300573242 8 24.880919739585657 29.918317636631446 18.972498358063344 26.228264265719552 9 24.027112678301567 16.82554662143636 19.91047459939934 39.236866100862734 10-11 27.09354894246526 22.66637760079578 23.934016464001733 26.30605699273722 12-13 25.62983414845658 20.101768190426395 23.97833281258967 30.290064848527358 14-15 24.800257608702584 21.326684818302972 21.88813150796738 31.984926065027068 16-17 25.72548094397011 22.945666243695282 22.844918285754368 28.483934526580246 18-19 25.038418129531266 22.196114189520937 23.61073029516601 29.154737385781786 20-21 24.87709386776512 22.690926944977587 23.838050845836495 28.5939283414208 22-23 26.439324861152734 22.294949211551582 22.864366467508784 28.401359459786903 24-25 25.451931108801418 23.409872024587603 22.434274710349623 28.703922156261356 26-27 24.45114680507821 23.531662277541496 23.77364867019072 28.24354224718958 28-29 25.26924572937058 22.824194813393103 24.80695288438853 27.09960657284779 30-31 25.319300885689326 22.42343474019142 24.595573466303634 27.66169090781562 32-33 24.19895808757421 23.542502247699694 24.4320174459755 27.8265222187506 34-35 24.742231886091044 23.007199015475653 24.55317005362597 27.697399044807337 36-37 25.13725315156191 22.05838280398146 24.600036983427596 28.204327061029034 38-39 23.921582380584976 22.032239346541093 26.516479942866983 27.52969833000695 40-41 23.854948446377218 21.824366977625026 26.635081969303755 27.685602606693998 42-43 22.749271490240837 21.84636574059314 26.753365173088817 28.650997596077204 44-45 21.987285352649735 21.64136277554248 28.00665701696774 28.364694854840046 46-47 22.02108055373118 22.48847456114062 27.974455929144852 27.515988955983346 48-49 21.853061016279085 22.853845320002293 27.782205870162663 27.510887793555955 50-51 21.63434867720482 23.73220172546819 26.71733821344539 27.916111383881603 52-53 20.79010629547208 23.642612560337188 26.99726450164831 28.570016642542416 54-55 20.850682599297315 23.330166361659664 26.877706007256403 28.941445031786618 56-57 21.02348447652509 23.386916793664355 26.825419092375675 28.76417963743488 58-59 21.379928201138835 23.518590548821315 26.12687866247521 28.97460258756464 60-61 21.34485770945054 22.770632607905526 26.433267230770213 29.45124245187372 62-63 21.24124034764422 22.875525260318696 26.221568990033607 29.661665402003486 64-65 22.108756782951914 22.915059269130953 25.478712211545208 29.497471736371928 66-67 21.95891013664739 23.84060142705019 24.193219279843394 30.007269156459028 68-69 21.85274219362737 24.485579651463077 24.336689473113683 29.324988681795865 70-71 22.98488142985583 24.009577432457423 24.88889030587845 28.1166508318083 72-73 24.08386311030626 23.5562116217233 24.57166176742525 27.788263500545185 74-75 23.417842590880397 24.67942382370383 24.77602708717249 27.126706498243287 76 23.14078570654288 24.29747428695314 24.762955358452306 27.798784648051672 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 2.0 14 1.5 15 2.0 16 7.0 17 10.0 18 8.5 19 15.0 20 35.0 21 47.0 22 51.0 23 96.0 24 145.0 25 153.0 26 223.0 27 354.5 28 505.0 29 594.0 30 740.5 31 1046.0 32 1449.0 33 1693.0 34 1973.0 35 2583.0 36 3136.0 37 3359.0 38 3884.0 39 4896.5 40 5915.5 41 6670.0 42 6893.0 43 7060.0 44 7549.0 45 7937.5 46 8004.0 47 7984.5 48 7566.5 49 6801.0 50 6434.0 51 6161.0 52 5318.5 53 4519.5 54 4290.0 55 4241.5 56 3928.0 57 3617.0 58 3571.0 59 3555.5 60 3768.5 61 4031.0 62 4065.0 63 4252.0 64 4530.0 65 4539.0 66 4504.0 67 4551.0 68 4461.5 69 4117.5 70 3602.0 71 3341.0 72 3016.0 73 2330.5 74 1667.0 75 1364.0 76 1037.0 77 537.0 78 226.0 79 88.0 80 65.0 81 29.0 82 17.0 83 18.0 84 17.5 85 12.5 86 9.0 87 10.0 88 9.5 89 8.5 90 7.0 91 5.0 92 4.0 93 2.5 94 2.5 95 4.0 96 4.0 97 6.0 98 6.5 99 15.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 156827.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.19418212425156 #Duplication Level Percentage of deduplicated Percentage of total 1 75.662974502183 46.30133841749189 2 13.062551448905376 15.987043047434435 3 4.799466494388813 8.810982802706167 4 1.9923100167762509 4.876711280583063 5 1.1597495024434974 3.548496113551876 6 0.7325282122352009 2.6895878898403973 7 0.5501776615365378 2.356737041453321 8 0.4501453594389855 2.2037021686316773 9 0.3365670164323896 1.8536348970521657 >10 1.252487782513103 11.334782913656449 >50 0.0010420031468495034 0.036983427598564024 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005738807730811659 0.0 2 0.0 0.0 0.0 0.008289388944505729 0.0 3 0.0 0.0 0.0 0.008927034247929247 0.0 4 0.0 0.0 0.0 0.029969329260905328 0.0 5 0.0 0.0 0.0 0.031244619867752364 0.0 6 0.0 0.0 0.0 0.0325199104745994 0.0 7 0.0 0.0 0.0 0.0325199104745994 0.0 8 0.0 0.0 0.0 0.0325199104745994 0.0 9 0.0 0.0 0.0 0.04399752593622272 0.0 10 0.0 0.0 0.0 0.04909868836361086 0.0 11 0.0 0.0 0.0 0.05164926957730493 0.0 12 0.0 0.0 0.0 0.052286914880728445 0.0 13 0.0 0.0 0.0 0.05930101321838714 0.0 14 0.0 0.0 0.0 0.061851594432081214 0.0 15 0.0 0.0 0.0 0.0650398209491988 0.0 16 0.0 0.0 0.0 0.06631511155604583 0.0 17 0.0 0.0 0.0 0.07014098337658695 0.0 18 0.0 0.0 0.0 0.07460450050055156 0.0 19 0.0 0.0 0.0 0.08098095353478674 0.0 20 0.0 0.0 0.0 0.08990798778271598 0.0 21 0.0 0.0 0.0 0.09373385960325709 0.0 22 0.0 0.0 0.0 0.09947266733406875 0.0 23 0.0 0.0 0.0 0.1052114750648804 0.0 24 0.0 0.0 0.0 0.11222557340253911 0.0 25 0.0 0.0 0.0 0.11796438113335077 0.0 26 0.0 0.0 0.0 0.13645609493263278 0.0 27 0.0 0.0 0.0 0.1957571081510199 0.0 28 0.0 0.0 0.0 0.3073450362501355 0.0 29 0.0 0.0 0.0 0.4342364516314155 0.0 30 0.0 0.0 0.0 0.5643160935298132 0.0 31 0.0 0.0 0.0 0.7033227696761399 0.0 32 0.0 0.0 0.0 0.8716611297799486 0.0 33 0.0 0.0 0.0 1.0712441097515095 0.0 34 0.0 0.0 0.0 1.357546850988669 0.0 35 0.0 0.0 0.0 1.775204524731073 0.0 36 0.0 0.0 0.0 2.277669023828805 0.0 37 0.0 0.0 0.0 2.9484718830303454 0.0 38 0.0 0.0 0.0 3.7034439222837903 0.0 39 0.0 0.0 0.0 4.449488927289306 0.0 40 0.0 0.0 0.0 5.237618522320774 0.0 41 0.0 0.0 0.0 6.089512647694593 0.0 42 0.0 0.0 0.0 6.906973926683543 0.0 43 0.0 0.0 0.0 7.70403055596294 0.0 44 0.0 6.376453034235176E-4 0.0 8.49981189463549 0.0 45 0.0 6.376453034235176E-4 0.0 9.347242502885345 0.0 46 0.0 6.376453034235176E-4 0.0 10.227830666913222 0.0 47 0.0 6.376453034235176E-4 0.0 11.042103719385056 0.0 48 0.0 6.376453034235176E-4 0.0 11.779859335446064 0.0 49 0.0 6.376453034235176E-4 0.0 12.503586754831757 0.0 50 0.0 6.376453034235176E-4 0.0 13.220300075879791 0.0 51 0.0 6.376453034235176E-4 0.0 13.898754678722414 0.0 52 0.0 6.376453034235176E-4 0.0 14.58868689702666 0.0 53 0.0 6.376453034235176E-4 0.0 15.320066060053435 0.0 54 0.0 6.376453034235176E-4 0.0 16.006810051840564 0.0 55 0.0 6.376453034235176E-4 0.0 16.67506232982841 0.0 56 0.0 6.376453034235176E-4 0.0 17.37328393707716 0.0 57 0.0 6.376453034235176E-4 0.0 18.044724441582126 0.0 58 0.0 6.376453034235176E-4 0.0 18.670254484240598 0.0 59 0.0 6.376453034235176E-4 0.0 19.253699936873115 0.0 60 0.0 6.376453034235176E-4 0.0 19.86902765467681 0.0 61 0.0 6.376453034235176E-4 0.0 20.495195342638702 0.0 62 0.0 6.376453034235176E-4 0.0 21.18321462503268 0.0 63 0.0 6.376453034235176E-4 0.0 21.78897766328502 0.0 64 0.0 6.376453034235176E-4 0.0 22.396653637447635 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGAATC 20 0.006940901 52.500004 7 TCGATTC 20 0.006940901 52.500004 4 CCGAAAT 20 0.006940901 52.500004 47 GCATGTA 20 0.006940901 52.500004 2 GACGAAT 40 4.5765857E-5 43.750004 23 ACGAATT 40 4.5765857E-5 43.750004 24 CTATCGG 35 0.0012524554 40.000004 42 ATAAGAG 45 9.16559E-5 38.88889 3 GAGTGAC 40 0.0024130882 35.000004 25 AGGAGTG 40 0.0024130882 35.000004 5 AAGAGCA 40 0.0024130882 35.000004 16 TTCCCAG 40 0.0024130882 35.000004 38 AGTGACT 40 0.0024130882 35.000004 26 TACAGTA 45 0.0042972313 31.111113 54 TAGATCT 80 2.4507062E-6 30.625002 39 GTAGATC 70 3.4718512E-5 30.000002 38 ACAGTAG 50 0.007191692 27.999998 55 TTGTCTG 50 0.007191692 27.999998 9 CGGTGTA 50 0.007191692 27.999998 34 GGAGTGA 50 0.007191692 27.999998 6 >>END_MODULE