FastQCFastQC Report
Wed 25 May 2016
SRR1780324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156827
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT98356.271241559170296No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC31091.982439248343716No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG23831.5195087580582425No Hit
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG20841.3288528123346106Illumina PCR Primer Index 9 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC6530.416382383135557No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT3680.23465347165985448Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT3330.21233588604003137No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1730.11031263749226854No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGC252.3846529E-455.99999632
TCTTTAT1050.053.3333321
ACAACGC200.00694090152.5000048
CCATAGG200.00694090152.5000041
GGTATCT200.00694090152.50000435
GTATCTT200.00694090152.50000436
CTTTATA1250.044.82
ACTCGTA602.5806912E-740.83333238
TTGTGAT350.001252455440.00000445
TAAGGAA350.001252455440.00000416
CTTGAAA23150.036.73866357
CCGTCTT23850.036.3941347
TTGAAAA23300.036.35193358
CGTATGC24100.036.30705641
TATGCCG24100.036.30705643
TCGTATG24050.036.23700740
CTCGTAT23900.036.17154739
TGAAAAA23350.036.124259
ATCTCGT22250.036.0224737
TCTTCTG24000.036.02083250