Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780324_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 156827 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT | 9835 | 6.271241559170296 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC | 3109 | 1.982439248343716 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG | 2383 | 1.5195087580582425 | No Hit |
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG | 2084 | 1.3288528123346106 | Illumina PCR Primer Index 9 (96% over 25bp) |
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC | 653 | 0.416382383135557 | No Hit |
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT | 368 | 0.23465347165985448 | Illumina PCR Primer Index 9 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT | 333 | 0.21233588604003137 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT | 173 | 0.11031263749226854 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATGTCGC | 25 | 2.3846529E-4 | 55.999996 | 32 |
TCTTTAT | 105 | 0.0 | 53.333332 | 1 |
ACAACGC | 20 | 0.006940901 | 52.500004 | 8 |
CCATAGG | 20 | 0.006940901 | 52.500004 | 1 |
GGTATCT | 20 | 0.006940901 | 52.500004 | 35 |
GTATCTT | 20 | 0.006940901 | 52.500004 | 36 |
CTTTATA | 125 | 0.0 | 44.8 | 2 |
ACTCGTA | 60 | 2.5806912E-7 | 40.833332 | 38 |
TTGTGAT | 35 | 0.0012524554 | 40.000004 | 45 |
TAAGGAA | 35 | 0.0012524554 | 40.000004 | 16 |
CTTGAAA | 2315 | 0.0 | 36.738663 | 57 |
CCGTCTT | 2385 | 0.0 | 36.39413 | 47 |
TTGAAAA | 2330 | 0.0 | 36.351933 | 58 |
CGTATGC | 2410 | 0.0 | 36.307056 | 41 |
TATGCCG | 2410 | 0.0 | 36.307056 | 43 |
TCGTATG | 2405 | 0.0 | 36.237007 | 40 |
CTCGTAT | 2390 | 0.0 | 36.171547 | 39 |
TGAAAAA | 2335 | 0.0 | 36.1242 | 59 |
ATCTCGT | 2225 | 0.0 | 36.02247 | 37 |
TCTTCTG | 2400 | 0.0 | 36.020832 | 50 |