##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780324_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156827 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.75609429498747 34.0 34.0 34.0 34.0 34.0 2 33.74785591766724 34.0 34.0 34.0 34.0 34.0 3 33.75754174982624 34.0 34.0 34.0 34.0 34.0 4 33.77313855394798 34.0 34.0 34.0 34.0 34.0 5 33.76113169288452 34.0 34.0 34.0 34.0 34.0 6 37.51904965343978 38.0 38.0 38.0 37.0 38.0 7 37.51088141710292 38.0 38.0 38.0 37.0 38.0 8 37.42857416133702 38.0 38.0 38.0 37.0 38.0 9 37.451491133542056 38.0 38.0 38.0 37.0 38.0 10-11 37.3992169715674 38.0 38.0 38.0 37.0 38.0 12-13 37.37456560413704 38.0 38.0 38.0 37.0 38.0 14-15 37.29532861050712 38.0 38.0 38.0 36.0 38.0 16-17 37.33085183036084 38.0 38.0 38.0 36.5 38.0 18-19 37.296352031219115 38.0 38.0 38.0 36.0 38.0 20-21 37.321723937842336 38.0 38.0 38.0 36.5 38.0 22-23 37.30049034923833 38.0 38.0 38.0 36.0 38.0 24-25 37.30363712881073 38.0 38.0 38.0 36.0 38.0 26-27 37.197274703973164 38.0 38.0 38.0 36.0 38.0 28-29 37.19098433305489 38.0 38.0 38.0 36.0 38.0 30-31 37.18798421190229 38.0 38.0 38.0 36.0 38.0 32-33 37.129725748755 38.0 38.0 38.0 36.0 38.0 34-35 37.15560777162096 38.0 38.0 38.0 36.0 38.0 36-37 37.149317400702685 38.0 38.0 38.0 36.0 38.0 38-39 36.49275953757963 38.0 37.0 38.0 34.0 38.0 40-41 36.78270960995237 38.0 37.5 38.0 34.5 38.0 42-43 37.071371638812195 38.0 38.0 38.0 36.0 38.0 44-45 37.14880728445995 38.0 38.0 38.0 36.0 38.0 46-47 37.10006886569277 38.0 38.0 38.0 36.0 38.0 48-49 37.115939857294975 38.0 38.0 38.0 36.0 38.0 50-51 36.9922143508452 38.0 38.0 38.0 35.5 38.0 52-53 36.98556689855701 38.0 38.0 38.0 36.0 38.0 54-55 36.94331014429913 38.0 38.0 38.0 35.5 38.0 56-57 36.99993304724315 38.0 38.0 38.0 36.0 38.0 58-59 36.88809643747569 38.0 38.0 38.0 35.0 38.0 60-61 36.898773808081515 38.0 38.0 38.0 35.0 38.0 62-63 36.88180606655742 38.0 38.0 38.0 35.0 38.0 64-65 36.82898990607485 38.0 38.0 38.0 35.5 38.0 66-67 36.539792255160144 38.0 37.5 38.0 34.0 38.0 68-69 36.451854591365006 38.0 37.0 38.0 34.0 38.0 70-71 35.781437507572036 38.0 37.0 38.0 31.0 38.0 72-73 34.68733700191932 38.0 37.0 38.0 23.5 38.0 74-75 34.34795347739866 38.0 36.5 38.0 22.0 38.0 76 33.71974851269233 38.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10756936540127526 1101 2 -0.039082983058726484 1101 3 -0.042486521928012166 1101 4 0.02520715833747289 1101 5 0.02219672985152954 1101 6 -0.10129131916034595 1101 7 -0.08732055911517733 1101 8 0.12573824013072255 1101 9 0.05463296610545143 1101 10-11 0.154069191490656 1101 12-13 0.12075890936902312 1101 14-15 0.3044909779585794 1101 16-17 0.13916798859667523 1101 18-19 0.032079502624632994 1101 20-21 0.015795834081579585 1101 22-23 0.1832929416988378 1101 24-25 0.023332003132118473 1101 26-27 0.054292177961720256 1101 28-29 0.04359845891326586 1101 30-31 -0.03669515360948594 1101 32-33 -0.013452815023242692 1101 34-35 0.00806524697663491 1101 36-37 0.006949503059900053 1101 38-39 0.17045551536240566 1101 40-41 0.1178108492699721 1101 42-43 0.13697575971850284 1101 44-45 -0.02153318103021462 1101 46-47 -0.052310018092441624 1101 48-49 0.06927277007940091 1101 50-51 0.09971485667410462 1101 52-53 0.0747388268780611 1101 54-55 0.040504018255454355 1101 56-57 -0.24562068069050014 1101 58-59 0.12312122168554396 1101 60-61 -0.1806628290559047 1101 62-63 -0.041282564435270785 1101 64-65 -0.24242798252969067 1101 66-67 -0.2876439919963687 1101 68-69 -0.4748693930233898 1101 70-71 -0.021631711758125505 1101 72-73 0.49749940794860237 1101 74-75 0.5973672923816551 1101 76 -0.12516063533090005 1102 1 -0.01366393716300962 1102 2 0.031607861994942255 1102 3 0.057189830372031736 1102 4 0.015188358213720221 1102 5 -0.13777090537847414 1102 6 0.006845088826132439 1102 7 0.13424722841962478 1102 8 -0.12874166277497068 1102 9 -0.19681273961311518 1102 10-11 -0.023116025424698705 1102 12-13 -7.934097095372294E-4 1102 14-15 0.1178997497631471 1102 16-17 -0.011060468603297124 1102 18-19 0.1253066985504816 1102 20-21 0.16246654634449698 1102 22-23 -0.0328192403054004 1102 24-25 0.08939421012386362 1102 26-27 -0.014024853998485298 1102 28-29 0.14545229390053294 1102 30-31 0.24213566907440764 1102 32-33 0.11858235786650084 1102 34-35 0.32011755415638987 1102 36-37 0.27689786221365154 1102 38-39 0.2946017755656314 1102 40-41 0.07045952493214003 1102 42-43 0.2409892291742537 1102 44-45 0.1128698555467409 1102 46-47 0.14722116869511837 1102 48-49 0.09210659126404153 1102 50-51 0.12703453017068256 1102 52-53 0.227900341358918 1102 54-55 0.1518055103665361 1102 56-57 0.22262138875096582 1102 58-59 0.11653522896761359 1102 60-61 0.15591888008952282 1102 62-63 -0.012683768595088907 1102 64-65 0.13752489817281344 1102 66-67 0.23127511987626548 1102 68-69 0.3643835995279545 1102 70-71 0.4796236129604665 1102 72-73 0.38827425861850173 1102 74-75 0.1968651624942197 1102 76 0.5805817315808781 1103 1 -0.09963809521955369 1103 2 -0.014204957273257435 1103 3 0.0756929392641581 1103 4 0.027069180163195483 1103 5 0.17688596076580154 1103 6 0.0904615740444541 1103 7 0.10907560370566927 1103 8 0.021594652321070384 1103 9 0.06863348464317909 1103 10-11 0.1360500055948819 1103 12-13 0.220677593225993 1103 14-15 0.16424420114192628 1103 16-17 0.043374036632123136 1103 18-19 0.21723282480154893 1103 20-21 0.20080184440519133 1103 22-23 0.1977530732188555 1103 24-25 0.036548822610278364 1103 26-27 0.12325180272896574 1103 28-29 0.24754877357138128 1103 30-31 0.2381946571242466 1103 32-33 0.3017238413512402 1103 34-35 0.07154487080109107 1103 36-37 0.2130211084055489 1103 38-39 0.1641034282480689 1103 40-41 0.14136306837572477 1103 42-43 -0.10263580838671515 1103 44-45 0.20864453598960608 1103 46-47 0.13407483117806862 1103 48-49 0.05176033695897786 1103 50-51 0.2479577208879249 1103 52-53 -0.014703037972147115 1103 54-55 0.20346628285111024 1103 56-57 -0.15284956795569116 1103 58-59 0.15221236647541758 1103 60-61 -0.04306177312454196 1103 62-63 0.008980239911018373 1103 64-65 0.3854445986096735 1103 66-67 -0.34087660330520464 1103 68-69 -0.03844329643395383 1103 70-71 -0.06462320302548363 1103 72-73 0.22936826504432162 1103 74-75 0.1638461855393203 1103 76 0.5549015891959286 1104 1 -0.12891583502360504 1104 2 -0.15583175153162898 1104 3 0.014574233408609416 1104 4 0.04482949002876779 1104 5 -0.02517601234880118 1104 6 0.05429160366395536 1104 7 0.07047911247759941 1104 8 -0.044787211429209606 1104 9 0.09231769516949129 1104 10-11 -0.1259800695930835 1104 12-13 -0.09993074580339112 1104 14-15 -0.00339079544043841 1104 16-17 -0.09289276673994351 1104 18-19 6.345108348142503E-4 1104 20-21 -0.0029233777410837547 1104 22-23 -0.06777438178228579 1104 24-25 -0.04128211837855389 1104 26-27 0.02543339306846093 1104 28-29 -0.315227485257509 1104 30-31 -0.042319127336917006 1104 32-33 -0.008608808455100814 1104 34-35 -0.17477547551925454 1104 36-37 -0.175603863114155 1104 38-39 -0.3174755191203502 1104 40-41 -0.16773923283183478 1104 42-43 0.02383947072014081 1104 44-45 -0.17510460960163954 1104 46-47 -0.18847586374104708 1104 48-49 -0.02764841218660763 1104 50-51 -0.3364920398776263 1104 52-53 -0.4634385130234122 1104 54-55 -0.6115485269005418 1104 56-57 -0.38552215852301686 1104 58-59 -0.24210866337192982 1104 60-61 -0.3185117617324167 1104 62-63 -0.04046154637287458 1104 64-65 -0.20962831093692103 1104 66-67 -0.26929423830178933 1104 68-69 -0.26078893325327357 1104 70-71 -0.7596448480471238 1104 72-73 -0.6197830222648619 1104 74-75 -0.28337983405512546 1104 76 -0.10537410002712733 1105 1 0.02380125181112902 1105 2 0.0982858955271908 1105 3 0.06900000371673087 1105 4 -0.060912806890002 1105 5 0.0038716066988158104 1105 6 0.03594426472837853 1105 7 0.17440068110504825 1105 8 0.03316797064361765 1105 9 0.08584710693420305 1105 10-11 0.019145980827076414 1105 12-13 0.06527933823021925 1105 14-15 0.030894918845277175 1105 16-17 -0.003831142090085393 1105 18-19 0.028057480022489756 1105 20-21 -0.04090657101838957 1105 22-23 0.10934046415609089 1105 24-25 -0.037360549751106475 1105 26-27 0.010209303432596073 1105 28-29 -0.01840675696618632 1105 30-31 0.16677050851743047 1105 32-33 0.06657326437403555 1105 34-35 0.11368390823424335 1105 36-37 0.08589473632562061 1105 38-39 -0.11924022500271292 1105 40-41 0.09340922655191264 1105 42-43 -0.07106249006417187 1105 44-45 -0.17338192052601187 1105 46-47 -0.05804169007157611 1105 48-49 -0.060547571850477766 1105 50-51 -0.2500214516423327 1105 52-53 -0.2485645634435798 1105 54-55 -0.26140847087813057 1105 56-57 -0.2917406459179759 1105 58-59 -0.14049801911422577 1105 60-61 -0.11461820430945835 1105 62-63 -0.13261840911796696 1105 64-65 -0.18045464146913304 1105 66-67 -0.26563877611690856 1105 68-69 0.07073767739098002 1105 70-71 -0.1703451281591768 1105 72-73 0.24289204776314932 1105 74-75 0.13344089423619465 1105 76 -0.372404912352053 1106 1 -0.051066079369554984 1106 2 -0.04700120260561391 1106 3 0.02119430387116239 1106 4 0.013297497756916243 1106 5 -0.025818130771348535 1106 6 -0.011198792972024307 1106 7 -0.22649781671890423 1106 8 -0.0057089070764462235 1106 9 -0.08775390387584991 1106 10-11 -0.06449012755422245 1106 12-13 -0.10409030939016617 1106 14-15 -0.07788789738383883 1106 16-17 -0.004038919711867095 1106 18-19 -0.14669473112449793 1106 20-21 -0.11550435285189309 1106 22-23 -0.13043491138792263 1106 24-25 -0.2721492623721886 1106 26-27 -0.14474787753047735 1106 28-29 0.0615511259483128 1106 30-31 0.006910898802424015 1106 32-33 -0.2350777253774723 1106 34-35 -0.29892594716375953 1106 36-37 -0.22817517803630238 1106 38-39 0.12321660498465548 1106 40-41 0.13560773841489038 1106 42-43 0.020418627442502668 1106 44-45 0.020488639631224714 1106 46-47 -0.13856394193174992 1106 48-49 -0.12885025330491828 1106 50-51 -0.16668297066521376 1106 52-53 0.013973692909075908 1106 54-55 -0.07971536404112811 1106 56-57 -0.03199334843095869 1106 58-59 -0.030502230822776255 1106 60-61 -0.06894362899006268 1106 62-63 0.1321717074059663 1106 64-65 -0.12819576178360848 1106 66-67 -0.25309069244430304 1106 68-69 -0.10871199269185183 1106 70-71 -0.11045744038717231 1106 72-73 0.060342560187685024 1106 74-75 0.3036613069836278 1106 76 0.2776751101103869 1107 1 0.07570804351331617 1107 2 -0.01670131674902109 1107 3 0.05235891249763114 1107 4 0.07315937270647765 1107 5 0.06512764534615911 1107 6 -0.08977326866178714 1107 7 0.12700085160803098 1107 8 0.2190844241815526 1107 9 0.1461824294690075 1107 10-11 0.045724554285449415 1107 12-13 0.07408581734360808 1107 14-15 0.13239250338633468 1107 16-17 0.26550957970588485 1107 18-19 -0.08121504057995566 1107 20-21 -0.04647065172314058 1107 22-23 -0.01711875721361622 1107 24-25 0.11717198790163508 1107 26-27 0.04515388996286873 1107 28-29 0.10197403303097019 1107 30-31 0.039237118349767286 1107 32-33 -0.14832585469388704 1107 34-35 -0.16176650381463276 1107 36-37 0.11626984288998443 1107 38-39 0.11102689633858631 1107 40-41 0.3361600080239242 1107 42-43 0.057414349947201515 1107 44-45 0.178087246851085 1107 46-47 0.09166561313956123 1107 48-49 0.05829016614327287 1107 50-51 0.19829056881803098 1107 52-53 0.19287618676955276 1107 54-55 0.07209536543900441 1107 56-57 -0.0962191909591823 1107 58-59 0.10083474587582231 1107 60-61 -0.11098933247085796 1107 62-63 0.021132656525686855 1107 64-65 0.2389741735351265 1107 66-67 0.17330410538351515 1107 68-69 0.15906958986612096 1107 70-71 0.079865158854183 1107 72-73 0.2675013117586005 1107 74-75 0.8019376262623368 1107 76 0.20618559631517996 1108 1 -0.09892499253276554 1108 2 -0.1332355976854771 1108 3 -0.06692759809322268 1108 4 0.028368867318143032 1108 5 0.0010831451603579012 1108 6 -0.06292451355216144 1108 7 -0.051476015727530466 1108 8 0.01649941860741677 1108 9 -0.17402845867665917 1108 10-11 -0.28810232234034316 1108 12-13 -0.1815928337154773 1108 14-15 0.05103914961450329 1108 16-17 -0.11231813304030425 1108 18-19 0.08855804563334857 1108 20-21 -0.12236019092789974 1108 22-23 -0.03296440009729906 1108 24-25 -0.05794878504521961 1108 26-27 -0.12534499520993592 1108 28-29 0.11020749642747774 1108 30-31 0.08150184335905664 1108 32-33 -0.13015184874814167 1108 34-35 -0.004285548779328963 1108 36-37 0.02124824311295015 1108 38-39 -0.271562057581896 1108 40-41 -0.24968749290509606 1108 42-43 0.05813434251501093 1108 44-45 0.08646819259615768 1108 46-47 5.923597021748606E-4 1108 48-49 0.08986532407712389 1108 50-51 -0.1175016552622381 1108 52-53 -0.1722297218146167 1108 54-55 -0.09868222653423686 1108 56-57 -0.05616089478674979 1108 58-59 -0.11313659377486118 1108 60-61 0.15691177306611337 1108 62-63 -0.024035264321589977 1108 64-65 0.042729747671138796 1108 66-67 -0.27372418335672677 1108 68-69 0.03579989458555133 1108 70-71 0.08539865012136971 1108 72-73 -0.4241328391146837 1108 74-75 -0.48558908313937366 1108 76 -0.44674314764617407 1109 1 0.05825664162255606 1109 2 -0.09793701468952776 1109 3 -0.02717787413297401 1109 4 -0.0734092887363289 1109 5 0.07640749186628426 1109 6 -0.22293555514215058 1109 7 -0.05838851112687138 1109 8 0.04073340830193217 1109 9 -0.09165359669653128 1109 10-11 -0.0027059752663660674 1109 12-13 0.07833309561834056 1109 14-15 0.12716286142900657 1109 16-17 0.05055107012494631 1109 18-19 0.06173043245705401 1109 20-21 0.04290710756308869 1109 22-23 -0.11923257271416077 1109 24-25 -5.325171026342446E-4 1109 26-27 0.032342711819879355 1109 28-29 -0.09934117858172442 1109 30-31 0.0097257576027161 1109 32-33 -0.10856323998847728 1109 34-35 -0.051660870825706695 1109 36-37 0.0048871371136911534 1109 38-39 -0.1708248014170053 1109 40-41 -0.004569945978346368 1109 42-43 -0.12177456436757694 1109 44-45 -0.05331718649842543 1109 46-47 0.17354851538859606 1109 48-49 -0.11450760562475182 1109 50-51 -0.07620495853791454 1109 52-53 0.1407993543723478 1109 54-55 -0.1613662530340534 1109 56-57 -0.0069165536016200235 1109 58-59 0.22278652915483832 1109 60-61 0.1267946862056064 1109 62-63 -0.19676138688323874 1109 64-65 -0.018861621486017555 1109 66-67 -0.2332211009128642 1109 68-69 -0.31595525815643555 1109 70-71 0.029874404630021445 1109 72-73 -0.6845073330418074 1109 74-75 -0.6770629053697732 1109 76 -0.3948591763543092 1110 1 0.07978774210102557 1110 2 -0.13142983765602878 1110 3 0.023061360316788182 1110 4 0.05099262513927272 1110 5 0.018991223923698897 1110 6 -0.04350971782157842 1110 7 -0.015326310169932356 1110 8 0.15078125519188745 1110 9 0.09734161861447177 1110 10-11 -0.10438061641468721 1110 12-13 -0.014968339788360652 1110 14-15 -0.06661704287721193 1110 16-17 0.16897212275652862 1110 18-19 0.2435486142752339 1110 20-21 0.01898366258701145 1110 22-23 -0.07497419950841788 1110 24-25 0.017410066629416576 1110 26-27 0.0215771615806446 1110 28-29 -0.0407287383903423 1110 30-31 -0.1744847687130786 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-0.03001699626855725 2115 54-55 -0.015075863761204289 2115 56-57 0.015536042535181593 2115 58-59 -0.05335204961532014 2115 60-61 0.09886037230638323 2115 62-63 0.03731623140490825 2115 64-65 -0.20887875185403004 2115 66-67 0.09574103506682263 2115 68-69 0.05107173694072742 2115 70-71 -0.01855137362031911 2115 72-73 -0.0825255266740399 2115 74-75 -0.23880311447840796 2115 76 0.1599698153167921 2116 1 0.05252915676621228 2116 2 0.06929140117772903 2116 3 0.09051840418036505 2116 4 0.09417267557884657 2116 5 0.160127764334284 2116 6 -0.0027691516726662257 2116 7 0.10479766748580488 2116 8 0.019826910081462756 2116 9 -0.14733747724429946 2116 10-11 0.05554702400296918 2116 12-13 0.1072121113394644 2116 14-15 0.2133579730324655 2116 16-17 -0.03694038578755965 2116 18-19 0.03454255681182161 2116 20-21 0.1254017454116294 2116 22-23 0.22762791706828978 2116 24-25 0.2055980129843391 2116 26-27 0.15281434544940709 2116 28-29 0.12611077418419825 2116 30-31 0.1455708562427276 2116 32-33 0.023492997784636316 2116 34-35 0.16939529623772387 2116 36-37 0.03527462621261179 2116 38-39 0.3083865498451601 2116 40-41 0.18554483941775146 2116 42-43 0.11727132622096548 2116 44-45 0.031422483994418826 2116 46-47 0.08808407057750856 2116 48-49 0.08281956810895963 2116 50-51 0.06276904386621851 2116 52-53 -0.037330138639177335 2116 54-55 0.1279424419007782 2116 56-57 -5.467890648915841E-4 2116 58-59 0.2622837832618998 2116 60-61 0.1395342152790775 2116 62-63 0.06832334689477904 2116 64-65 0.10486265786439475 2116 66-67 0.33674024445683415 2116 68-69 0.3108448926908949 2116 70-71 0.5325554803600951 2116 72-73 0.6588294572096842 2116 74-75 0.6490914303126871 2116 76 0.6433122710730359 2117 1 -0.13546345407122828 2117 2 0.0566682484683696 2117 3 -0.034235290400914664 2117 4 0.019234251933532676 2117 5 -0.008628393301179926 2117 6 -0.02523220585985797 2117 7 0.09631244581093057 2117 8 -0.6485967352387334 2117 9 -0.10768230483050445 2117 10-11 -0.00651578387879681 2117 12-13 -0.16832360294625204 2117 14-15 -0.05785508115472027 2117 16-17 -0.09640467150184406 2117 18-19 0.02783689178719584 2117 20-21 -0.023994806312508388 2117 22-23 0.008237522979619882 2117 24-25 -0.21169878504522188 2117 26-27 -0.008614225979165724 2117 28-29 -0.7067155804955974 2117 30-31 -0.12970007971786401 2117 32-33 -0.38688261797890533 2117 34-35 0.03552214352836103 2117 36-37 -0.160722910733206 2117 38-39 -0.29872551912035306 2117 40-41 -0.4738701852127889 2117 42-43 -0.15157719594652264 2117 44-45 -0.08867603817306247 2117 46-47 -0.029071101836287028 2117 48-49 -0.2215769836151793 2117 50-51 0.049757960122377654 2117 52-53 -0.06290279873769578 2117 54-55 0.04410623500422162 2117 56-57 -0.20909358709444348 2117 58-59 -0.09270390146716778 2117 60-61 -0.1525593807800405 2117 62-63 0.1274551202937957 2117 64-65 -0.2186164061750162 2117 66-67 -0.2823299525874958 2117 68-69 -0.4147770284913719 2117 70-71 -0.11166865757093802 2117 72-73 -0.30615206988391463 2117 74-75 0.05763207070678078 2117 76 -0.6004931476461763 2118 1 0.07409168377338915 2118 2 -0.21698463373714105 2118 3 -0.12013754604001292 2118 4 -0.08161161272812478 2118 5 0.11103952649831683 2118 6 0.12634674150856284 2118 7 0.04516582926957824 2118 8 0.01248095899685353 2118 9 0.1513402515604696 2118 10-11 0.09720288780039255 2118 12-13 0.1303950687329447 2118 14-15 0.1444788787450264 2118 16-17 0.14100447636783286 2118 18-19 -0.07292438640829602 2118 20-21 0.10208915358723658 2118 22-23 0.004030129495909307 2118 24-25 -0.15005404820310986 2118 26-27 -0.10595758437515457 2118 28-29 0.03508579795051503 2118 30-31 0.11977360449266428 2118 32-33 -0.05046344504658151 2118 34-35 -0.04921156574481955 2118 36-37 0.029721951422182258 2118 38-39 0.042549543536289036 2118 40-41 0.2085765566418445 2118 42-43 0.12383947072014934 2118 44-45 0.09563473876929152 2118 46-47 0.1290053392664703 2118 48-49 0.177928028916142 2118 50-51 -0.031604821832509344 2118 52-53 0.19884218873097836 2118 54-55 0.31100786407690606 2118 56-57 0.25658310463487766 2118 58-59 0.10482116118947005 2118 60-61 -0.054226047446711334 2118 62-63 0.02219509523114027 2118 64-65 -0.13061828587426305 2118 66-67 -0.16634937614639256 2118 68-69 -0.24543805606029423 2118 70-71 -0.20024635180652695 2118 72-73 -0.3304973079791509 2118 74-75 -0.18344249069672713 2118 76 -0.613018210302819 2119 1 0.0014161985342369121 2119 2 -0.006452098926168048 2119 3 0.1291021785817108 2119 4 0.01603946285164426 2119 5 -0.03900680521185507 2119 6 0.07293156585230065 2119 7 -0.16119996113695834 2119 8 -0.07120219429580743 2119 9 0.013781715020613206 2119 10-11 0.02908905483087665 2119 12-13 -0.08163935977007242 2119 14-15 -0.6910901928172493 2119 16-17 -0.32513606108000914 2119 18-19 -0.2783386665254639 2119 20-21 -0.06970820581623371 2119 22-23 0.10634236168930045 2119 24-25 -0.20869320191867047 2119 26-27 0.06835847873548317 2119 28-29 0.08500588352424643 2119 30-31 -0.24786385143001866 2119 32-33 -0.1838894418002539 2119 34-35 0.02169149836707618 2119 36-37 -0.04028246408307723 2119 38-39 -0.003455668003731205 2119 40-41 0.2509716262016042 2119 42-43 -6.435731177347748E-4 2119 44-45 -8.844748976315486E-4 2119 46-47 0.06095991553344504 2119 48-49 0.08808182531782194 2119 50-51 0.2033342628519037 2119 52-53 0.13732362806130993 2119 54-55 0.08385499430944066 2119 56-57 -0.038907482876076926 2119 58-59 0.03896483302414566 2119 60-61 0.07397908075841997 2119 62-63 0.22794209299851076 2119 64-65 -0.023414793271783196 2119 66-67 0.13248704493111063 2119 68-69 0.07480423701731809 2119 70-71 0.304032334984889 2119 72-73 0.3008981782550464 2119 74-75 0.47963269105417083 2119 76 -0.4640353312690024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 8.0 22 8.0 23 15.0 24 26.0 25 66.0 26 127.0 27 222.0 28 426.0 29 759.0 30 1178.0 31 1863.0 32 2969.0 33 5007.0 34 8102.0 35 14250.0 36 21698.0 37 100101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.80233633239175 16.753492702149504 12.379245920664172 34.06492504479458 2 19.676458773042906 18.238568613822874 36.042263130710914 26.04270948242331 3 18.811174096297194 18.120604232689523 32.27888054990531 30.78934112110797 4 15.179146447996839 22.50122746720909 32.0780222793269 30.24160380546717 5 16.039967607618586 29.786325058822776 37.88505805760487 16.28864927595376 6 29.678563002544205 37.24996333539505 16.092892167802738 16.978581494258005 7 27.89188086235151 24.40459869792829 21.47908204582119 26.224438393899007 8 24.138700606400683 36.412735051999974 16.20256715999158 23.24599718160776 9 22.789443144356518 15.450145701951831 17.074228289771533 44.686182863920116 10-11 26.788116842125397 25.691366920236945 23.099976407123773 24.420539830513878 12-13 25.415266503854568 18.64028515497969 25.485407487231154 30.45904085393459 14-15 22.846512399012926 18.262161490049543 20.281584165991827 38.6097419449457 16-17 28.02259814955333 24.291416656570615 19.29036454181997 28.395620652056085 18-19 29.92214350845199 22.828339507865355 19.78549611992833 27.464020863754328 20-21 25.04798280908262 20.971835206947784 19.805263124334456 34.17491885963514 22-23 24.67240972536617 24.40842456974883 19.522467432266126 31.396698272618874 24-25 28.20018236655678 24.779215313689605 20.872043716962 26.14855860279161 26-27 28.62421649333342 27.368374068240804 20.262454806889117 23.74495463153666 28-29 28.107086152256944 23.005286079565384 20.81911915677785 28.06850861139982 30-31 26.264610048014692 20.549076370777993 28.07902975890631 25.107283822301007 32-33 22.776371415636337 28.26235278364057 24.746695403215007 24.214580397508083 34-35 23.205825527492077 27.17771812251717 21.283643760321883 28.332812589668873 36-37 28.26936688197823 25.330778501150952 21.334017739292342 25.06583687757848 38-39 23.676407761418634 23.285212367768302 26.10328578624854 26.935094084564522 40-41 22.958419149763753 19.152633156280487 27.82843515466087 30.06051253929489 42-43 26.96091871935317 19.1041721132203 27.126387675591573 26.808521491834952 44-45 22.20376593316202 24.02233033852589 29.284179382376756 24.48972434593533 46-47 25.446829946374027 21.02731034834563 26.647834875372222 26.878024829908114 48-49 25.230668188513455 20.029714271139536 23.83135557015055 30.90826197019646 50-51 22.828658330517065 20.586059798376553 27.295363680998808 29.28991819010757 52-53 19.728426865271924 20.514643524393122 33.64662972574875 26.1102998845862 54-55 19.902822855758256 20.976617546723457 30.28018134632429 28.840378251193993 56-57 23.34036868651444 22.097597990142003 25.17391775650876 29.388115566834795 58-59 21.012006861063465 22.074323936567044 29.917679991328022 26.995989211041465 60-61 23.845064944174155 25.59380718881315 25.578184878879274 24.982942988133424 62-63 20.283497101902096 32.52883750884733 21.862306873178724 25.32535851607185 64-65 19.324159742901415 34.450062808062384 20.92050476002219 25.305272689014007 66-67 19.552436761527034 34.02315927742034 20.528671721068438 25.895732239984188 68-69 19.181646017586257 34.699382121700985 20.805409782754243 25.31356207795851 70-71 19.70419634374183 33.379775166266015 21.17046171896421 25.74556677102795 72-73 19.708341038214083 31.008691105485664 22.127567319402907 27.155400536897346 74-75 19.57156612062974 30.266790794952396 22.666696423447494 27.49494666097037 76 19.631823601803262 30.518341867152976 22.607076587577392 27.242757943466366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 2.0 15 3.0 16 3.5 17 5.0 18 9.0 19 12.0 20 20.0 21 29.0 22 41.0 23 70.5 24 88.5 25 89.0 26 166.5 27 283.0 28 425.5 29 529.0 30 611.0 31 839.0 32 1147.0 33 1309.0 34 1567.5 35 2059.0 36 2525.0 37 2758.0 38 3186.0 39 6350.0 40 10658.0 41 11564.5 42 10899.0 43 9832.5 44 8228.5 45 7371.5 46 7052.0 47 6924.0 48 6476.0 49 5791.0 50 5426.0 51 5102.5 52 4254.5 53 3592.5 54 3455.0 55 3431.5 56 3309.0 57 3146.0 58 3082.0 59 3149.0 60 3321.5 61 3555.0 62 3683.0 63 3875.0 64 4196.0 65 4288.5 66 4407.0 67 4562.0 68 4368.0 69 4050.0 70 3678.5 71 3431.0 72 3077.5 73 2359.5 74 1665.5 75 1336.0 76 1011.5 77 479.0 78 174.5 79 78.0 80 52.5 81 17.5 82 5.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 156827.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.768145791222175 #Duplication Level Percentage of deduplicated Percentage of total 1 61.06422948473282 26.116038692317012 2 20.956583969465647 17.92548476984193 3 7.529222328244274 9.660326346866292 4 3.1190362595419847 5.335815899047995 5 1.9083969465648856 4.0809299419105125 6 1.1390744274809161 2.9229660708934047 7 0.8528148854961831 2.553131794907765 8 0.6694298664122137 2.2904219298972754 9 0.5263000954198473 2.0257991289765154 >10 2.2021111641221376 14.253923112729314 >50 0.016400286259541985 0.4986386272771908 >100 0.0089456106870229 0.8180989242923731 >500 0.0014909351145038167 0.416382383135557 >1k 0.00447280534351145 4.83080081873657 >5k 0.0014909351145038167 6.271241559170296 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 9835 6.271241559170296 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 3109 1.982439248343716 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 2383 1.5195087580582425 No Hit CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG 2084 1.3288528123346106 Illumina PCR Primer Index 9 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 653 0.416382383135557 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 368 0.23465347165985448 Illumina PCR Primer Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT 333 0.21233588604003137 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT 173 0.11031263749226854 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007014098337658694 0.0 2 0.0 0.0 0.0 0.0108399701581998 0.0 3 0.0 0.0 0.0 0.011477615461623318 0.0 4 0.0 0.0 0.0 0.033157555778022915 0.0 5 0.0 0.0 0.0 0.036983427598564024 0.0 6 0.0 0.0 0.0 0.03825871820541106 0.0 7 0.0 0.0 0.0 0.03825871820541106 0.0 8 0.0 0.0 0.0 0.03825871820541106 0.0 9 0.0 0.0 0.0 0.04909868836361086 0.0 10 0.0 0.0 0.0 0.054199850790998996 0.0 11 0.0 0.0 0.0 0.05675043200469307 0.0 12 0.0 0.0 0.0 0.05738807730811659 0.0 13 0.0 0.0 0.0 0.06440217564577529 0.0 14 0.0 0.0 0.0 0.06759040216289287 0.0 15 0.0 0.0 0.0 0.07141627398343398 0.0 16 0.0 0.0 0.0 0.072691564590281 0.0 17 0.0 0.0 0.0 0.07651743641082212 0.0 18 0.0 0.0 0.0 0.08098095353478674 0.0 19 0.0 0.0 0.0 0.08799505187244544 0.0 20 0.0 0.0 0.0 0.09819737672722172 0.0 21 0.0 0.0 0.0 0.1013856032443393 0.0 22 0.0 0.0 0.0 0.1052114750648804 0.0 23 0.0 0.0 0.0 0.11158792809911558 0.0 24 0.0 0.0 0.0 0.11796438113335077 0.0 25 0.0 0.0019129359102705529 0.0 0.12370318886416243 0.0 26 0.0 0.0019129359102705529 0.0 0.14283254796686795 0.0 27 0.0 0.0019129359102705529 0.0 0.20468414239894916 0.0 28 0.0 0.0019129359102705529 0.0 0.32073558762202936 0.0 29 0.0 0.0019129359102705529 0.0 0.452090520127274 0.0 30 0.0 0.0019129359102705529 0.0 0.5796195808119775 0.0 31 0.0 0.0019129359102705529 0.0 0.7237274193856925 0.0 32 0.0 0.0019129359102705529 0.0 0.9016304590408539 0.0 33 0.0 0.0019129359102705529 0.0 1.1107781185637677 0.0 34 0.0 0.0019129359102705529 0.0 1.4047326034420093 0.0 35 0.0 0.0019129359102705529 0.0 1.8262161490049544 0.0 36 0.0 0.0019129359102705529 0.0 2.3497229431156623 0.0 37 0.0 0.0019129359102705529 0.0 3.0339163536890967 0.0 38 0.0 0.0019129359102705529 0.0 3.805467170831553 0.0 39 0.0 0.0019129359102705529 0.0 4.5559756929610336 0.0 40 0.0 0.0019129359102705529 0.0 5.364509937702054 0.0 41 0.0 0.0019129359102705529 0.0 6.229156969144344 0.0 42 0.0 0.0019129359102705529 0.0 7.0587335088983405 0.0 43 0.0 0.0019129359102705529 0.0 7.867267753639361 0.0 44 0.0 0.0019129359102705529 0.0 8.683453742021463 0.0 45 0.0 0.0019129359102705529 0.0 9.535985512698707 0.0 46 0.0 0.0019129359102705529 0.0 10.429326582795055 0.0 47 0.0 0.0019129359102705529 0.0 11.244874925873733 0.0 48 0.0 0.0019129359102705529 0.0 11.990282285575825 0.0 49 0.0 0.0019129359102705529 0.0 12.729313192243682 0.0 50 0.0 0.0019129359102705529 0.0 13.449852385112257 0.0 51 0.0 0.0019129359102705529 0.0 14.146798701754163 0.0 52 0.0 0.0019129359102705529 0.0 14.846933244913185 0.0 53 0.0 0.0019129359102705529 0.0 15.588514732794735 0.0 54 0.0 0.0019129359102705529 0.0 16.28354811352637 0.0 55 0.0 0.0019129359102705529 0.0 16.960727425762144 0.0 56 0.0 0.0019129359102705529 0.0 17.664050195438286 0.0 57 0.0 0.0019129359102705529 0.0 18.346330670101448 0.0 58 0.0 0.0019129359102705529 0.0 18.97058542215307 0.0 59 0.0 0.0019129359102705529 0.0 19.55913203721298 0.0 60 0.0 0.0019129359102705529 0.0 20.17892327214064 0.0 61 0.0 0.0019129359102705529 0.0 20.805090960102532 0.0 62 0.0 0.0019129359102705529 0.0 21.491197306586237 0.0 63 0.0 0.0019129359102705529 0.0 22.10588737908651 0.0 64 0.0 0.0019129359102705529 0.0 22.721852742193626 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTCGC 25 2.3846529E-4 55.999996 32 TCTTTAT 105 0.0 53.333332 1 ACAACGC 20 0.006940901 52.500004 8 CCATAGG 20 0.006940901 52.500004 1 GGTATCT 20 0.006940901 52.500004 35 GTATCTT 20 0.006940901 52.500004 36 CTTTATA 125 0.0 44.8 2 ACTCGTA 60 2.5806912E-7 40.833332 38 TTGTGAT 35 0.0012524554 40.000004 45 TAAGGAA 35 0.0012524554 40.000004 16 CTTGAAA 2315 0.0 36.738663 57 CCGTCTT 2385 0.0 36.39413 47 TTGAAAA 2330 0.0 36.351933 58 CGTATGC 2410 0.0 36.307056 41 TATGCCG 2410 0.0 36.307056 43 TCGTATG 2405 0.0 36.237007 40 CTCGTAT 2390 0.0 36.171547 39 TGAAAAA 2335 0.0 36.1242 59 ATCTCGT 2225 0.0 36.02247 37 TCTTCTG 2400 0.0 36.020832 50 >>END_MODULE