FastQCFastQC Report
Wed 25 May 2016
SRR1780323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684212
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACG250.002342329835.22981334
TAATGTA300.005719572329.3581836
CGGTAAG300.00575239529.32382828
GCCATTT300.005762682329.31310733
CTATAAC300.005772984629.3023974
TTATGCA300.005772984629.3023972
TATTGCG554.975085E-627.98683211
TATAGAA801.3214958E-724.7238982
TAAACTG450.001400234824.43652327
TATCGAT551.6000993E-423.98345242
TCTATCG651.8215947E-523.67597231
TTAGAGG752.081093E-623.4419174
TCGATTA602.884434E-421.9848344
GACGAAA1451.2732926E-1121.24381623
ATCGATT654.952129E-420.2936943
TAACCGG550.00447867720.00229522
GTATAGA1001.1333868E-619.7791181
ATTGCGC808.9733796E-519.25220912
TACCGTG809.05437E-519.2296987
CTATATC1152.0407424E-719.110264
Produced by FastQC (version 0.11.5)